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Information on EC 2.7.7.41 - phosphatidate cytidylyltransferase and Organism(s) Sus scrofa

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Sus scrofa
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Word Map
The taxonomic range for the selected organisms is: Sus scrofa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
cdp-diacylglycerol synthase, cdp-dg, phosphatidate cytidylyltransferase, tam41, cdp-dag synthase, tamm41, ctp:phosphatidate cytidylyltransferase, cdp-diglyceride synthetase, cdp-dg synthase, tbcds, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CDP-DAG synthase
-
-
-
-
CDP-DG
-
-
-
-
CDP-DG synthetase
-
-
-
-
CDP-diacylglyceride synthetase
-
-
-
-
CDP-diacylglycerol synthase
CDP-diglyceride pyrophosphorylase
-
-
-
-
CDP-diglyceride synthetase
-
-
-
-
CDPdiglyceride pyrophosphorylase
-
-
-
-
CTP-diacylglycerol synthetase
-
-
-
-
CTP:1,2-diacylglycerophosphate-cytidyl transferase
-
-
-
-
CTP:phosphatidate cytidylyltransferase
-
-
-
-
cytidine diphosphoglyceride pyrophosphorylase
-
-
-
-
cytidylyltransferase, phosphatidate
-
-
-
-
DAG synthetase
-
-
-
-
phosphatidate cytidyltransferase
-
-
-
-
phosphatidic acid cytidylyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
CTP:phosphatidate cytidylyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9067-83-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
up to 10 mM, never more than 15% as effective as Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
2 mM, 45% inhibition, even in presence of optimal Mg2+ concentrations
cardiolipin
-
2 mM, 27% inhibition
Co2+
-
2 mM, 58% inhibition, even in presence of optimal Mg2+ concentrations
F-
-
20 mM NaF, 70% inhibition
FeSO4
-
2 mM, 97% inhibition, even in presence of optimal Mg2+ concentrations
lysolecithin
-
1 mM, 89% inhibition
oleic acid
-
2 mM, 84% inhibition
palmitic acid
-
2 mM, 76% inhibition
palmitoyl-CoA
-
0.5 mM, 70% inhibition
phosphatidylethanolamine
-
2 mM, 16% inhibition
phosphatidylinositol
-
2 mM, 23% inhibition
phosphatidylserine
-
2 mM, 46% inhibition
SDS
-
-
sodium deoxycholate
-
-
Triton X-100
-
-
Tween 20
-
-
ZWT-12
-
2 mM, 86% inhibition
-
ZWT-14
-
2 mM, 92% inhibition
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
stimulates only in presence of either lecithin or sphingomyelin
cationic detergent
-
the phosphatidate must be emulsified in a cationic detergent for optimal activity
-
GTP
-
stimulates only in presence of either lecithin or sphingomyelin
ITP
-
stimulates only in presence of either lecithin or sphingomyelin
lecithin
-
stimulates
sphingomyelin
-
stimulates
UTP
-
stimulates only in presence of either lecithin or sphingomyelin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
CTP
-
pH 7.0, 37°C
0.65
phosphatidate
-
pH 7.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00011
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
Tris-HCl buffer
7.5
-
phosphate buffer
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high expression of the gene CDS1 in testis
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8D1PP19_PIG
467
7
53492
TrEMBL
other Location (Reliability: 5)
A0A8D0WDG6_PIG
461
7
53036
TrEMBL
other Location (Reliability: 5)
A0A8D1FBL4_PIG
211
2
24059
TrEMBL
Mitochondrion (Reliability: 4)
A0A8D0JQM3_PIG
424
7
48928
TrEMBL
other Location (Reliability: 3)
A0A8D2AFR1_PIG
461
7
53040
TrEMBL
other Location (Reliability: 5)
A0A480TLQ8_PIG
445
8
51469
TrEMBL
other Location (Reliability: 2)
A0A8D2C578_PIG
320
0
35874
TrEMBL
other Location (Reliability: 5)
A0A8D1SE29_PIG
455
7
52280
TrEMBL
other Location (Reliability: 5)
A0A8D1PLD3_PIG
454
7
51875
TrEMBL
other Location (Reliability: 5)
A0A480LPD0_PIG
404
8
46937
TrEMBL
other Location (Reliability: 2)
A0A8D1J339_PIG
449
6
51489
TrEMBL
other Location (Reliability: 5)
A0A8D2BQJ4_PIG
441
7
50294
TrEMBL
other Location (Reliability: 5)
A0A4X1TR74_PIG
460
6
52856
TrEMBL
other Location (Reliability: 5)
Q30GF0_PIG
375
7
43536
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8D0U7Q6_PIG
279
4
31941
TrEMBL
Secretory Pathway (Reliability: 2)
A0A480MCC2_PIG
337
0
37989
TrEMBL
other Location (Reliability: 5)
A0A8D0U825_PIG
472
7
54351
TrEMBL
Mitochondrion (Reliability: 5)
A0A8D1N4N2_PIG
447
8
51696
TrEMBL
other Location (Reliability: 2)
A0A8D1G8I7_PIG
328
0
37272
TrEMBL
other Location (Reliability: 5)
A0A8D1BWM0_PIG
261
3
30336
TrEMBL
other Location (Reliability: 5)
F1RW13_PIG
467
7
53496
TrEMBL
other Location (Reliability: 5)
F1SQA3_PIG
332
0
37849
TrEMBL
other Location (Reliability: 5)
A0A8D1AXB9_PIG
417
7
48227
TrEMBL
other Location (Reliability: 2)
Q30GF1_PIG
461
7
53002
TrEMBL
other Location (Reliability: 5)
A0A8D2ADP3_PIG
467
7
53496
TrEMBL
other Location (Reliability: 5)
A0A8D0J179_PIG
461
7
53327
TrEMBL
other Location (Reliability: 4)
A0A8D1CMI2_PIG
472
7
54374
TrEMBL
other Location (Reliability: 5)
A0A4X1UIR3_PIG
432
0
47720
TrEMBL
other Location (Reliability: 5)
A0A286ZZ71_PIG
472
7
54378
TrEMBL
other Location (Reliability: 5)
A0A8D1IQJ0_PIG
462
6
53106
TrEMBL
other Location (Reliability: 5)
A0A8D0JRE0_PIG
415
7
48138
TrEMBL
Mitochondrion (Reliability: 3)
A0A8D1BAJ5_PIG
414
7
47730
TrEMBL
other Location (Reliability: 5)
A0A8D1GNS8_PIG
440
7
50913
TrEMBL
other Location (Reliability: 2)
A0A8D0RGD2_PIG
305
5
35201
TrEMBL
Secretory Pathway (Reliability: 1)
A0A4X1TQV4_PIG
461
7
53064
TrEMBL
other Location (Reliability: 5)
A0A8D0QDG4_PIG
332
0
37849
TrEMBL
other Location (Reliability: 5)
A0A8D1IR15_PIG
404
5
46493
TrEMBL
other Location (Reliability: 5)
A0A4X1UJI1_PIG
316
0
35785
TrEMBL
other Location (Reliability: 5)
A0A4X1UHZ2_PIG
445
8
51501
TrEMBL
other Location (Reliability: 2)
I3LAQ3_PIG
431
8
49965
TrEMBL
other Location (Reliability: 2)
A0A8D2BQ85_PIG
407
7
47329
TrEMBL
Mitochondrion (Reliability: 3)
A0A8D1N4U4_PIG
427
7
49382
TrEMBL
other Location (Reliability: 3)
A0A5G2QW23_PIG
454
7
51879
TrEMBL
other Location (Reliability: 5)
A0A8D0TUW1_PIG
259
6
30323
TrEMBL
other Location (Reliability: 3)
A0A8D2A5A9_PIG
472
7
54378
TrEMBL
other Location (Reliability: 5)
A0A8D1KGP7_PIG
268
4
30602
TrEMBL
Secretory Pathway (Reliability: 2)
A0A5G2R6W5_PIG
320
0
35874
TrEMBL
other Location (Reliability: 5)
D0G6R8_PIG
445
8
51501
TrEMBL
other Location (Reliability: 2)
A0A8D1DPN5_PIG
445
8
51469
TrEMBL
other Location (Reliability: 2)
A0A286ZWR5_PIG
461
7
53040
TrEMBL
other Location (Reliability: 5)
A0A5G2QR66_PIG
316
0
35785
TrEMBL
other Location (Reliability: 5)
A0A8D0U7L4_PIG
459
7
52734
TrEMBL
Mitochondrion (Reliability: 5)
A0A8D1N5D2_PIG
417
7
48195
TrEMBL
other Location (Reliability: 2)
A0A4X1UHI6_PIG
431
8
49997
TrEMBL
other Location (Reliability: 2)
A0A8D0RIV7_PIG
294
5
33862
TrEMBL
Secretory Pathway (Reliability: 1)
A0A5G2QL26_PIG
404
5
46497
TrEMBL
other Location (Reliability: 5)
A0A8D2BQJ0_PIG
397
5
45824
TrEMBL
other Location (Reliability: 5)
A0A8D0IZN0_PIG
446
7
51586
TrEMBL
Secretory Pathway (Reliability: 5)
A0A8D1YMG0_PIG
422
7
49048
TrEMBL
other Location (Reliability: 2)
A0A8D1W8Z6_PIG
449
6
51827
TrEMBL
other Location (Reliability: 5)
A0A4X1UGZ8_PIG
337
0
37989
TrEMBL
other Location (Reliability: 5)
A0A8D1SE74_PIG
478
8
54837
TrEMBL
other Location (Reliability: 2)
A0A8D2ABD7_PIG
404
5
46497
TrEMBL
other Location (Reliability: 5)
A0A8D0WUR0_PIG
478
8
54805
TrEMBL
other Location (Reliability: 2)
A0A8D1KCG0_PIG
318
0
35689
TrEMBL
other Location (Reliability: 5)
A0A8D2A5E7_PIG
454
7
51879
TrEMBL
other Location (Reliability: 5)
A0A5G2QAE0_PIG
432
0
47720
TrEMBL
other Location (Reliability: 5)
A0A4X1TQS8_PIG
472
7
54402
TrEMBL
other Location (Reliability: 5)
A0A8D1B7X4_PIG
437
7
50424
TrEMBL
Secretory Pathway (Reliability: 5)
A0A8D1GHZ5_PIG
478
8
54809
TrEMBL
other Location (Reliability: 2)
A0A8D0WD72_PIG
474
7
54653
TrEMBL
other Location (Reliability: 5)
A0A8D2BQJ6_PIG
454
7
51911
TrEMBL
other Location (Reliability: 5)
A0A8D0ISQ4_PIG
433
7
50362
TrEMBL
other Location (Reliability: 3)
A0A8D0IWE5_PIG
383
5
44586
TrEMBL
Secretory Pathway (Reliability: 5)
A0A287BFG8_PIG
455
7
52280
TrEMBL
other Location (Reliability: 5)
A0A8D2C799_PIG
447
8
51728
TrEMBL
other Location (Reliability: 2)
A0A4X1TLD1_PIG
404
5
46521
TrEMBL
other Location (Reliability: 5)
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 24 h, enzyme in isolated microsomes, 75% loss of activity
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sribney, M.; Hegadorn, C.A.
Biosynthesis of CDP-diacylglycerol in hog mesenteric lymph node lymphocytes
J. Biochem.
60
668-674
1982
Sus scrofa
Manually annotated by BRENDA team
Heacock, A.M.; Agranoff, B.W.
CDP-diacylglycerol synthase from mammalian tissues
Biochim. Biophys. Acta
1348
166-172
1997
Cavia porcellus, Drosophila sp. (in: flies), Escherichia coli, Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Mercade, A.; Sanchez, A.; Folch, J.M.
Characterization and physical mapping of the porcine CDS1 and CDS2 genes
Anim. Biotechnol.
18
23-35
2007
Sus scrofa
Manually annotated by BRENDA team