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Information on EC 2.7.7.41 - phosphatidate cytidylyltransferase and Organism(s) Arabidopsis thaliana

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Arabidopsis thaliana
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
cdp-diacylglycerol synthase, cdp-dg, phosphatidate cytidylyltransferase, tam41, cdp-dag synthase, tamm41, ctp:phosphatidate cytidylyltransferase, cdp-diglyceride synthetase, cdp-dg synthase, tbcds, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CDP-DAG synthase
CDP-DG
-
-
-
-
CDP-DG synthetase
-
-
-
-
CDP-diacylglyceride synthetase
-
-
-
-
CDP-diacylglycerol synthase
CDP-diglyceride pyrophosphorylase
-
-
-
-
CDP-diglyceride synthetase
-
-
-
-
CDPdiglyceride pyrophosphorylase
-
-
-
-
CTP-diacylglycerol synthetase
-
-
-
-
CTP:1,2-diacylglycerophosphate-cytidyl transferase
-
-
-
-
CTP:phosphatidate cytidylyltransferase
-
-
-
-
cytidine diphosphoglyceride pyrophosphorylase
-
-
-
-
cytidinediphosphate diacylglycerol synthase
-
cytidylyltransferase, phosphatidate
-
-
-
-
DAG synthetase
-
-
-
-
phosphatidate cytidyltransferase
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-
-
-
phosphatidic acid cytidylyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
CTP:phosphatidate cytidylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9067-83-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + 1,2-dioleoyl phosphatidic acid
diphosphate + CDP-dioleoylglycerol
show the reaction diagram
-
-
-
?
CTP + 1,2-dipalmitoyl phosphatidic acid
diphosphate + CDP-dipalmitoylglycerol
show the reaction diagram
best substrate
-
-
?
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
show the reaction diagram
best substrate
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
isoforms of CDS are located in plastids, mitochondria and the endomembrane system of plants and are encoded by five genes in Arabidopsis. Two genes have previously been shown to code for the plastidial isoforms which are indispensable for the biosynthesis of plastidial phosphatidylglycerol, and thus biogenesis and function of thylakoid membranes. the extraplastidial CDS isoforms, encoded by CDS1 and CDS2 which are constitutively expressed contrary to CDS3. These closely related CDS genes code for membrane proteins located in the endoplasmic reticulum and possess very similar enzymatic properties
malfunction
the seedling lethal phenotype of the cds1cds2 double mutant shows that plant cells require at least one catalytically active microsomal CDS isoform for cell division and expansion. According to the altered glycerolipid composition of the double mutant in comparison with wild-type seedlings, it is likely that the drastic decrease in the level of phosphatidylinositol and the increase in phosphatidic acid cause defects in cell division and expansion. The cds1cds2 double mutant is seedling lethal, phenotypes, overview. The seedling-lethal phenotype of cds1cds2 double mutants can be rescued by inducing the expression of a functional CDS1 or CDS2 gene
metabolism
the enzyme is critical for maintaining phosphoinositide levels during phospholipase C signaling
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDS1_ARATH
421
7
48660
Swiss-Prot
other Location (Reliability: 3)
CDS2_ARATH
423
7
48247
Swiss-Prot
other Location (Reliability: 2)
CDS3_ARATH
471
8
54924
Swiss-Prot
Chloroplast (Reliability: 4)
CDS4_ARATH
391
6
42159
Swiss-Prot
Chloroplast (Reliability: 2)
CDS5_ARATH
399
6
43251
Swiss-Prot
Chloroplast (Reliability: 4)
A0A1I9LR93_ARATH
275
0
30972
TrEMBL
other Location (Reliability: 4)
A0A178V7Z6_ARATH
423
7
48263
TrEMBL
other Location (Reliability: 2)
A0A654ELT7_ARATH
421
7
48710
TrEMBL
other Location (Reliability: 3)
A0A7G2F2P1_ARATH
376
7
43867
TrEMBL
Secretory Pathway (Reliability: 2)
F4JL62_ARATH
365
7
41821
TrEMBL
Secretory Pathway (Reliability: 1)
A0A178V702_ARATH
399
6
43251
TrEMBL
Chloroplast (Reliability: 4)
A0A5S9X745_ARATH
391
6
42189
TrEMBL
Chloroplast (Reliability: 2)
A0A178WPS5_ARATH
421
7
48678
TrEMBL
other Location (Reliability: 3)
A0A5S9XVF5_ARATH
436
7
49612
TrEMBL
other Location (Reliability: 5)
F4JBM0_ARATH
320
0
36039
TrEMBL
Secretory Pathway (Reliability: 3)
A0A654FCM3_ARATH
391
6
42174
TrEMBL
Chloroplast (Reliability: 2)
A0A5S9WRU3_ARATH
421
7
48660
TrEMBL
other Location (Reliability: 3)
A0A654FIA1_ARATH
332
0
37362
TrEMBL
other Location (Reliability: 5)
A0A5S9XNA4_ARATH
399
6
43171
TrEMBL
Chloroplast (Reliability: 2)
A0A1I9LR92_ARATH
225
0
25299
TrEMBL
other Location (Reliability: 3)
F4JL60_ARATH
447
7
50966
TrEMBL
Secretory Pathway (Reliability: 2)
A0A178V9I7_ARATH
332
0
37419
TrEMBL
other Location (Reliability: 5)
A0A178W0G1_ARATH
382
6
41396
TrEMBL
Chloroplast (Reliability: 2)
A0A5S9XIP9_ARATH
332
0
37298
TrEMBL
other Location (Reliability: 5)
A0A654FRU8_ARATH
423
7
48247
TrEMBL
other Location (Reliability: 2)
Q9SN75_ARATH
332
0
37362
TrEMBL
other Location (Reliability: 5)
A0A178V2S1_ARATH
471
8
54924
TrEMBL
Chloroplast (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48600
-
x * 48600, calculation from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 48600, calculation from nucleotide sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transient expression of GFP-tagged CDS1 in tobacco leaf epidermal cells
transient expression of GFP-tagged CDS2 in tobacco leaf epidermal cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kopka, J.; Ludewig, M.; Mueller-Roeber, B.
Complementary DNAs encoding eukaryotic-type cytidine-5'-diphosphate-diacylglycerol synthases of two plant species
Plant Physiol.
113
997-1002
1997
Arabidopsis thaliana, Solanum tuberosum
Manually annotated by BRENDA team
Zhou, Y.; Peisker, H.; Weth, A.; Baumgartner, W.; Doermann, P.; Frentzen, M.
Extraplastidial cytidinediphosphate diacylglycerol synthase activity is required for vegetative development in Arabidopsis thaliana
Plant J.
75
867-879
2013
Arabidopsis thaliana (O04928), Arabidopsis thaliana (O49639), Arabidopsis thaliana (Q1PE48), Arabidopsis thaliana
Manually annotated by BRENDA team
Blunsom, N.J.; Cockcroft, S.
CDP-diacylglycerol synthases (CDS) gateway to phosphatidylinositol and cardiolipin synthesis
Front. Cell Dev. Biol.
8
63
2020
Aeropyrum pernix, Saccharomyces cerevisiae, Drosophila melanogaster, Schizosaccharomyces pombe, Streptococcus mitis, Streptococcus oralis, Thermotoga maritima, Toxoplasma gondii, Trypanosoma brucei brucei, Eimeria falciformis, Arabidopsis thaliana (O04928), Arabidopsis thaliana (O49639), Arabidopsis thaliana (Q1PE48), Arabidopsis thaliana (Q94A03), Arabidopsis thaliana (Q9M001), Escherichia coli (P0ABG1), Danio rerio (Q3B7H2), Homo sapiens (Q92903), Homo sapiens (Q95674)
Manually annotated by BRENDA team