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Information on EC 2.7.7.12 - UDP-glucose-hexose-1-phosphate uridylyltransferase and Organism(s) Bos taurus

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Bos taurus
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
uridyltransferase, galt enzyme, uridyl transferase, hexose-1-phosphate uridylyltransferase, udp-glucose-hexose-1-phosphate uridylyltransferase, udpglucose:alpha-d-galactose-1-phosphate uridylyltransferase, hexose 1-phosphate uridylyltransferase, galactose-1-phosphate:uridyltransferase, udp-glucose:alpha-d-galactose-1-phosphate uridylyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexose 1-phosphate uridyltransferase
-
-
-
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hexose 1-phosphate uridylyltransferase
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-
-
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hexose-1-phosphate uridylyltransferase
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-
-
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UDPglucose:alpha-D-galactose-1-phosphate uridylyltransferase
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-
-
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uridyl transferase
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-
-
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uridyltransferase
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-
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uridylyltransferase, hexose 1-phosphate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-21-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-glucose + alpha-D-galactose 1-phosphate
alpha-D-glucose 1-phosphate + CDP-galactose
show the reaction diagram
-
low activity
-
-
?
TDP-glucose + alpha-D-galactose 1-phosphate
alpha-D-glucose 1-phosphate + TDP-galactose
show the reaction diagram
-
8.7% activity compared to UDP-glucose
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-
r
UDP-glucose + alpha-D-galactose 1-phosphate
alpha-D-glucose 1-phosphate + UDP-galactose
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + alpha-D-galactose 1-phosphate
alpha-D-glucose 1-phosphate + UDP-galactose
show the reaction diagram
-
important step in galactose metabolism
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-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cl-
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Cu2+
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p-hydroxymercuribenzoate
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complete inhibition at 0.001 mM
UDP
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UDP-glucose
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product inhibition
UMP
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-
UTP
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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cysteine
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activation
DTT
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most effective in activation
glutathione
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12.4
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purified enzyme
25.7
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purified enzyme
additional information
-
UDP-glucose consumed/h/g Hgb
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A3Q1M1I6_BOVIN
341
0
39204
TrEMBL
other Location (Reliability: 3)
Q58CX1_BOVIN
360
0
41288
TrEMBL
other Location (Reliability: 3)
Q0V7N7_BOVIN
331
0
37812
TrEMBL
other Location (Reliability: 4)
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
t1/2: 18 h, sulfhydryl compounds stabilize
642828
7 - 8
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fairly stable
642828
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
SH-reagents stabilize
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
about 2000fold
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about 500fold
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mayes, J.S.; Hansen, R.G.
Galactose 1-phosphate uridyl transferase
Methods Enzymol.
9
708-713
1966
Bos taurus, Homo sapiens
-
Manually annotated by BRENDA team
Mayes, J.S.
Purification, properties, and isozyme pattern of galactose-1-phosphate uridyl transferase from calf liver
Arch. Biochem. Biophys.
172
715-720
1976
Bos taurus
Manually annotated by BRENDA team
Kelly, S.; Sweeney, P.; Schedlbauer, L.
Galactose-l-phosphate uridyl transferase activity in red cells of various animal species
Experientia
37
550-553
1981
Bos taurus, Capra hircus, Cavia porcellus, Cavia porcellus Hartley, Chlorocebus aethiops, Equus caballus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Rattus norvegicus
Manually annotated by BRENDA team
Ellis, G.; Goldberg, D.M.
Galactose-1-phosphate uridylytransferase. UDPglucose:alpha-D-galactose-1-phosphate uridylyltransferase, EC 2.7.7.12
Methods Enzym. Anal. , 3rd. Ed. (Bergmeyer, H. U. , ed. )
3
560-571
1983
Bos taurus, Homo sapiens
-
Manually annotated by BRENDA team