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Information on EC 2.7.7.1 - nicotinamide-nucleotide adenylyltransferase and Organism(s) Bos taurus

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IUBMB Comments
Nicotinate nucleotide can also act as acceptor. See also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase.
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This record set is specific for:
Bos taurus
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The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
nmnat2, nmnat, nmnat1, nmnat3, nicotinamide mononucleotide adenylyltransferase, nmn adenylyltransferase, nicotinamide mononucleotide adenylyltransferase 1, nicotinamide mononucleotide adenylyltransferase 2, namnat, hnmnat-2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenosine triphosphate-nicotinamide mononucleotide transadenylase
-
-
-
-
adenylyltransferase, nicotinamide mononucleotide
-
-
-
-
ATP:NMN adenylyltransferase
-
-
-
-
diphosphopyridine nucleotide pyrophosphorylase
-
-
-
-
NAD+ pyrophosphorylase
-
-
-
-
nicotinamide adenine dinucleotide pyrophosphorylase
-
-
-
-
nicotinamide mononucleotide adenylyltransferase
NMN adenylyltransferase
-
-
-
-
NMNAT
PNAT
-
-
-
-
pyridine nucleotide adenylyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:nicotinamide-nucleotide adenylyltransferase
Nicotinate nucleotide can also act as acceptor. See also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-70-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + beta-NMNH
diphosphate + ?
show the reaction diagram
-
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag2+
-
IC50: 0.037 mM
Cd2+
-
IC50: 0.2 mM
Cr3+
-
IC50: 0.1 mM
Cu2+
-
IC50: 0.025 mM
Hg2+
-
IC50: 0.017 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
ATP
-
37°C, pH 7.4
0.625
beta-NMNH
-
37°C, pH 7.4
0.167
diphosphate
-
37°C, pH 7.4
0.37
NAD+
-
37°C, pH 7.4
0.15
nicotinic acid mononucleotide
-
37°C, pH 7.4
0.91
nicotinic acid-adenine dinucleotide
-
37°C, pH 7.4
0.11
NMN
-
37°C, pH 7.4
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037
Ag2+
Bos taurus
-
IC50: 0.037 mM
0.2
Cd2+
Bos taurus
-
IC50: 0.2 mM
0.1
Cr3+
Bos taurus
-
IC50: 0.1 mM
0.025
Cu2+
Bos taurus
-
IC50: 0.025 mM
0.017
Hg2+
Bos taurus
-
IC50: 0.017 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16.7
-
purification step: TSK phenyl-5PW
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 11.3
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
activity is high during mid luteal phase, when corpora lutea are mature with respect to structure and function, while it dramatically decreases following PGF2alpha analog injection
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NMNA1_BOVIN
281
0
32200
Swiss-Prot
other Location (Reliability: 2)
NMNA2_BOVIN
307
0
34468
Swiss-Prot
other Location (Reliability: 4)
A0A3Q1MVV1_BOVIN
247
0
28065
TrEMBL
Mitochondrion (Reliability: 5)
A0A3Q1NCV5_BOVIN
250
0
28139
TrEMBL
other Location (Reliability: 4)
G5E6K1_BOVIN
228
0
26086
TrEMBL
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
133000
-
gel filtration
33000
-
alpha4, 4 * 33000, SDS-PAGE, no disulfide interchain bonds
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
alpha4, 4 * 33000, SDS-PAGE, no disulfide interchain bonds
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
inactivation below pH 3 is irreversible
642792
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.5% v/v Triton X-100, 50 mM sodium phosphte buffer, pH 6.8, 0.5 M NaCl, 100% activity after 3 months
-
4°C, 0.5% (v/v) Triton X-100, 50 mM sodium phosphate buffer, pH 6.8, 0.5 M NaCl, 80% activity after 3 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ferro, A.M.; Kuehl, L.
Adenosine triphosphate: nicotinamide mononucleotide adenylyltransferase of pig liver. Purification and properties
Biochim. Biophys. Acta
410
285-298
1975
Bos taurus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Balducci, E.; Orsomando, G.; Polzonetti, V.; Vita, A.; Emanuelli, M.; Raffaelli, N.; Ruggieri, S.; Magni, G.; Natalini, P.
NMN adenylyltransferase from bull testis: purification and properties
Biochem. J.
310
395-400
1995
Bos taurus
Manually annotated by BRENDA team
Balducci, E.; Emanuelli, M.; Raffaelli, N.; Ruggieri, S.; Amici, A.; Magni, G.; Orsomando, G.; Polzonetti, V.; Natalini, P.
Assay methods for nicotinamide mononucleotide adenylyltransferase of wide applicability
Anal. Biochem.
228
64-68
1995
Bos taurus
Manually annotated by BRENDA team
Magni, G.; Amici, A.; Emanuelli, M.; Orsomando, G.; Raffaelli, N.; Ruggieri, S.
Structure and function of nicotinamide mononucleotide adenylyltransferase
Curr. Med. Chem.
11
873-885
2004
Bacillus subtilis, Bos taurus, Haemophilus influenzae, Homo sapiens, Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, Saccharolobus solfataricus, Saccharomyces cerevisiae
Manually annotated by BRENDA team