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Information on EC 2.7.4.6 - nucleoside-diphosphate kinase and Organism(s) Bos taurus

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IUBMB Comments
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
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This record set is specific for:
Bos taurus
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The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
kinase, nucleoside diphosphate (phosphorylating)
-
-
-
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NDK
-
-
-
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nucleoside 5'-diphosphate kinase
-
-
-
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nucleoside diphosphate (UDP) kinase
-
-
-
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nucleoside diphosphokinase
-
-
-
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nucleotide phosphate kinase
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-
-
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UDP kinase
-
-
-
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uridine diphosphate kinase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-51-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + adenosine 5'-methylene diphosphonate
ADP + ?
show the reaction diagram
-
-
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
best substrate
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
reverse reaction at 21% the rate of ATP plus ADP
-
r
ATP + dTDP
ADP + dTTP
show the reaction diagram
ATP + UDP
ADP + UTP
show the reaction diagram
-
reverse reaction at 32% the rate of the reaction ATP plus ADP
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r
CTP + CDP
CDP + CTP
show the reaction diagram
-
reaction at 15% the rate of ATP
-
?
GTP + ADP
GDP + ATP
show the reaction diagram
NDP + NTP
NTP + NDP
show the reaction diagram
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
UTP + CDP
UDP + CTP
show the reaction diagram
UTP + dCDP
UDP + dCTP
show the reaction diagram
UTP + dTDP
UDP + dTDP
show the reaction diagram
UTP + UDP
UDP + UTP
show the reaction diagram
-
reaction at 31% the rate of ATP
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NDP + NTP
NTP + NDP
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Fe2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Ni2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Zn2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
additional information
-
no activation by Fe3+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag2+
-
-
AMP
-
kinetics, substrate inhibition
MgADP-
-
in excess
p-chloromercuribenzoate
-
bovine liver mitochondrial and human erythrocytic enzymes: substrates protect
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 0.54
CDP
0.56
dCDP
-
cosubstrate ATP, pH 7.5, 37°C
0.21 - 0.39
dTDP
-
-
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
360
-
25°C, pH 8.0
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 10.3
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about half-maximal activity at pH 6.5 and 10.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KAD4_BOVIN
223
0
25348
Swiss-Prot
Mitochondrion (Reliability: 4)
KAD5_BOVIN
562
0
63273
Swiss-Prot
other Location (Reliability: 2)
NDK7_BOVIN
377
0
42599
Swiss-Prot
other Location (Reliability: 2)
KCY_BOVIN
196
0
22279
Swiss-Prot
other Location (Reliability: 3)
NDKA1_BOVIN
152
0
17261
Swiss-Prot
other Location (Reliability: 2)
NDKA2_BOVIN
152
0
17298
Swiss-Prot
other Location (Reliability: 2)
NDKB_BOVIN
152
0
17316
Swiss-Prot
other Location (Reliability: 2)
KAD1_BOVIN
194
0
21664
Swiss-Prot
other Location (Reliability: 1)
A0A3Q1LW70_BOVIN
454
0
52060
TrEMBL
other Location (Reliability: 4)
Q56JV4_BOVIN
152
0
17260
TrEMBL
other Location (Reliability: 2)
A0A452DIX8_BOVIN
228
0
26040
TrEMBL
Mitochondrion (Reliability: 3)
E1B7Q2_BOVIN
724
0
83016
TrEMBL
other Location (Reliability: 2)
A0A3Q1NHP5_BOVIN
468
0
53329
TrEMBL
other Location (Reliability: 4)
A0A3Q1M447_BOVIN
169
0
18649
TrEMBL
other Location (Reliability: 1)
A0A3Q1N2Q9_BOVIN
230
0
25195
TrEMBL
Mitochondrion (Reliability: 4)
A5PK70_BOVIN
169
0
19079
TrEMBL
Secretory Pathway (Reliability: 2)
F1MCI2_BOVIN
186
0
21160
TrEMBL
Secretory Pathway (Reliability: 5)
G1K159_BOVIN
368
0
41508
TrEMBL
other Location (Reliability: 2)
F1MUF5_BOVIN
679
0
78183
TrEMBL
other Location (Reliability: 1)
A0A3Q1MKM8_BOVIN
562
0
63190
TrEMBL
other Location (Reliability: 2)
A1A4N9_BOVIN
152
0
17325
TrEMBL
other Location (Reliability: 2)
A6H755_BOVIN
426
0
49669
TrEMBL
other Location (Reliability: 2)
A0A8J8YPU5_BOVIN
169
0
19079
TrEMBL
Secretory Pathway (Reliability: 2)
A0A8J8XHA9_BOVIN
255
0
27397
TrEMBL
Mitochondrion (Reliability: 2)
E1BLG0_BOVIN
498
0
56685
TrEMBL
other Location (Reliability: 4)
A0A452DIS4_BOVIN
547
0
61392
TrEMBL
other Location (Reliability: 2)
A0A3Q1LVZ0_BOVIN
223
0
25324
TrEMBL
Mitochondrion (Reliability: 3)
A0A3Q1M363_BOVIN
506
0
56529
TrEMBL
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
103000
108000
-
gel filtration
109000
-
liver mitochondrial enzyme, gel filtration, sedimentation equilibrium
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
overview on enzyme structure
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
inactivation below
642637
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
-
1 h, 0.0018 mg enzyme/ml, in 0.1 M phosphate buffer, pH 7, more than 50% loss of activity, 2 mg/ml bovine serum albumin plus 2 mM Mg-ADP or 5 mM free ADP protect, not MgCl2 or free ADP alone
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in dilute solutions, bovine serum albumin stabilizes, not DTT
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 months
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0-2°C, 2 mg bovine serum albumin/ml, 0.04% NaN3, at least 1 month
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
liver, partial: thymus and heart
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Colomb, M.G.; Chruy, A.; Vignais, P.V.
Nucleoside diphosphokinase from beef heart cytosol. I. Physical and kinetic properties
Biochemistry
11
3370-3378
1972
Bos taurus
Manually annotated by BRENDA team
Colomb, M.G.; Chruy, A.; Vignais, P.V.
Nucleoside diphosphokinase from beef heart cytosol. II. Characterization of the phosphorylated intermediate
Biochemistry
11
3378-3386
1972
Bos taurus
Manually annotated by BRENDA team
Nakamura, H.; Sugino, Y.
Metabolism of deoxyribonucleotides. 3. Purification and some properties of nucleoside diphosphokinase of calf thymus
J. Biol. Chem.
241
4917-4922
1966
Bos taurus
Manually annotated by BRENDA team
Parks, R.E.; Agarwal, R.P.
Nucleoside diphosphokinases
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
8
307-333
1973
Anguilla rostrata, Avian myeloblastosis virus, Papio sp., Bacillus subtilis, Beta vulgaris subsp. vulgaris, Bos taurus, Saccharomyces cerevisiae, Vicia faba, Canis lupus familiaris, Gallus gallus, Columba livia, Oryctolagus cuniculus, Streptococcus pneumoniae, Escherichia coli, Felis catus, Helianthus tuberosus, Homo sapiens, Hordeum vulgare, Impatiens holstii, Micrococcus luteus, Platyrrhini, Mus musculus, Myxine glutinosa, Phoca vitulina, Pisum sativum, Rattus norvegicus, Saccharomyces pastorianus, Saccharum officinarum, Schistosoma mansoni, Solanum tuberosum, Squalus acanthias, Sus scrofa, Triticum aestivum
-
Manually annotated by BRENDA team
Cuello, F.; Schulze, R.A.; Heemeyer, F.; Meyer, H.E.; Lutz, S.; Jakobs, K.H.; Niroomand, F.; Wieland, T.
Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gb subunits
J. Biol. Chem.
278
7220-7226
2003
Bos taurus
Manually annotated by BRENDA team
Janin, J.; Deville-Bonne, D.
Nucleoside-diphosphate kinase: structural and kinetic analysis of reaction pathway and phosphohistidine intermediate
Methods Enzymol.
354
118-134
2002
Bos taurus, Dictyostelium discoideum, Drosophila melanogaster, Homo sapiens, Myxococcus xanthus, Rattus norvegicus
Manually annotated by BRENDA team