Information on EC 2.7.4.6 - nucleoside-diphosphate kinase and Organism(s) Bos taurus

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Bos taurus


The taxonomic range for the selected organisms is: Bos taurus

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.4.6
-
RECOMMENDED NAME
GeneOntology No.
nucleoside-diphosphate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenosine deoxyribonucleotides de novo biosynthesis
-
-
adenosine deoxyribonucleotides de novo biosynthesis II
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CMP phosphorylation
-
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guanosine deoxyribonucleotides de novo biosynthesis I
-
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guanosine deoxyribonucleotides de novo biosynthesis II
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guanosine ribonucleotides de novo biosynthesis
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ppGpp biosynthesis
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purine deoxyribonucleosides salvage
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pyrimidine deoxyribonucleotide phosphorylation
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pyrimidine deoxyribonucleotides biosynthesis from CTP
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pyrimidine deoxyribonucleotides de novo biosynthesis I
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pyrimidine deoxyribonucleotides de novo biosynthesis II
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pyrimidine deoxyribonucleotides de novo biosynthesis III
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pyrimidine deoxyribonucleotides de novo biosynthesis IV
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superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
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UTP and CTP de novo biosynthesis
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non-pathway related
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purine metabolism
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pyrimidine metabolism
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Purine metabolism
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Pyrimidine metabolism
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Drug metabolism - other enzymes
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-51-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + adenosine 5'-methylene diphosphonate
ADP + ?
show the reaction diagram
-
-
-
?
ATP + ADP
ADP + ATP
show the reaction diagram
-
best substrate
-
?
ATP + CDP
ADP + CTP
show the reaction diagram
-
reverse reaction at 21% the rate of ATP plus ADP
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r
ATP + dTDP
ADP + dTTP
show the reaction diagram
ATP + UDP
ADP + UTP
show the reaction diagram
-
reverse reaction at 32% the rate of the reaction ATP plus ADP
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r
CTP + CDP
CDP + CTP
show the reaction diagram
-
reaction at 15% the rate of ATP
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?
GTP + ADP
GDP + ATP
show the reaction diagram
NDP + NTP
NTP + NDP
show the reaction diagram
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
UTP + CDP
UDP + CTP
show the reaction diagram
UTP + dCDP
UDP + dCTP
show the reaction diagram
UTP + dTDP
UDP + dTDP
show the reaction diagram
UTP + UDP
UDP + UTP
show the reaction diagram
-
reaction at 31% the rate of ATP
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NDP + NTP
NTP + NDP
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Co2+
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in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+; requirement
Fe2+
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requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Ni2+
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requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
Zn2+
-
requirement, in decreasing order of activity: Mn2+, Mg2+, Co2+, Zn2+, Ni2+, Ca2+, Fe2+
additional information
-
no activation by Fe3+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag2+
-
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AMP
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kinetics, substrate inhibition
MgADP-
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in excess
p-chloromercuribenzoate
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bovine liver mitochondrial and human erythrocytic enzymes: substrates protect
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 0.54
CDP
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cosubstrate ATP; pH 7.5, 37°C
0.56
dCDP
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cosubstrate ATP, pH 7.5, 37°C
0.21 - 0.39
dTDP
-
-
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
360
-
25°C, pH 8.0
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 10.3
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about half-maximal activity at pH 6.5 and 10.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
103000
108000
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gel filtration
109000
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liver mitochondrial enzyme, gel filtration, sedimentation equilibrium
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
overview on enzyme structure
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
inactivation below
642637
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
-
1 h, 0.0018 mg enzyme/ml, in 0.1 M phosphate buffer, pH 7, more than 50% loss of activity, 2 mg/ml bovine serum albumin plus 2 mM Mg-ADP or 5 mM free ADP protect, not MgCl2 or free ADP alone
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in dilute solutions, bovine serum albumin stabilizes, not DTT
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 months
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0-2°C, 2 mg bovine serum albumin/ml, 0.04% NaN3, at least 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
liver, partial: thymus and heart
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