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Information on EC 2.7.4.4 - nucleoside-phosphate kinase and Organism(s) Homo sapiens

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EC Tree
IUBMB Comments
Many nucleotides can act as acceptors; other nucleoside triphosphates can act instead of ATP.
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This record set is specific for:
Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
nucleoside monophosphate kinase, ampk1, nmp kinase, ampk2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AMPK1
-
isoform
AMPK2
-
isoform
kinase, nucleoside monophosphate (phosphorylating)
-
-
-
-
NMP kinase
-
-
NMP-kinase
-
-
-
-
nucleoside monophosphate kinase
-
-
thymidylate kinase
-
-
TMPK
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-phosphate phosphotransferase
Many nucleotides can act as acceptors; other nucleoside triphosphates can act instead of ATP.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-50-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dAMP
ADP + dADP
show the reaction diagram
-
100% activity
-
-
?
ATP + TMP
ADP + TDP
show the reaction diagram
-
-
-
-
?
ATP + [2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
ADP + (2-((2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)ethyl)boronic phosphoric anhydride
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + TMP
ADP + TDP
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid
-
about 30% inhibition at 1 mM
3-(thymin-1-yl)propylboronic acid
-
about 10% inhibition at 1 mM
[(E)-2-[(2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethenyl]boronic acid
-
about 15% inhibition at 1 mM
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
about 45% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
-
about 25% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
-
about 55% inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 0.14
dAMP
0.23
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.119
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
80 - 500
dAMP
1.25
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.014
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1000 - 3000
dAMP
5400
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.139
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KAD9_HUMAN
1911
0
221413
Swiss-Prot
other Location (Reliability: 1)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
El Amri, C.; Martin, A.; Vasseur, J.; Smietana, M.
Borononucleotides as substrates/binders for human NMP kinases: Enzymatic and spectroscopic evaluation
ChemBioChem
13
1605-1612
2012
Homo sapiens
Manually annotated by BRENDA team
Gallier, F.; Alexandre, J.A.; El Amri, C.; Deville-Bonne, D.; Peyrottes, S.; Perigaud, C.
5,6-nucleoside phosphonate analogues architecture: synthesis and comparative evaluation towards metabolic enzymes
ChemMedChem
6
1094-1106
2011
Homo sapiens
Manually annotated by BRENDA team