Information on EC 2.7.3.1 - guanidinoacetate kinase

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The expected taxonomic range for this enzyme is: Polychaeta

EC NUMBER
COMMENTARY
2.7.3.1
-
RECOMMENDED NAME
GeneOntology No.
guanidinoacetate kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + guanidinoacetate = ADP + phosphoguanidinoacetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:guanidinoacetate N-phosphotransferase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GK
Namalycastis sp.
Q6AW43
-
glycocyamine kinase
-
-
-
-
glycocyamine kinase
Namalycastis sp.
Q6AW42
-
glycocyamine kinase
Namalycastis sp.
Q6AW43
-
kinase, guanidinoacetate (phosphorylating)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9026-60-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Namalycastis sp.
-
SwissProt
Manually annotated by BRENDA team
Namalycastis sp.
alpha chain
SwissProt
Manually annotated by BRENDA team
Namalycastis sp.
beta chain
SwissProt
Manually annotated by BRENDA team
three isoforms
-
-
Manually annotated by BRENDA team
Perinereis sp.
polychaete
-
-
Manually annotated by BRENDA team
Polycelis cornuta
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphocreatine
ATP + ?
show the reaction diagram
-
poor substrate
-
-
?
ADP + phosphoguanidinoacetate
ATP + guanidinoacetate
show the reaction diagram
-
-
-
?
ATP + creatine
ADP + phosphocreatine
show the reaction diagram
Namalycastis sp.
Q6AW42, Q6AW43
0.5% of the activity with glycocyamine
-
-
r
ATP + glycocyamine
ADP + phosphoglycocyamine
show the reaction diagram
Namalycastis sp.
Q6AW42, Q6AW43
-
-
-
r
ATP + guanidine
ADP + phosphoguanidine
show the reaction diagram
Polycelis cornuta, Myxicola infundibulum
-
-
-
-
?
ATP + guanidine
ADP + phosphoguanidine
show the reaction diagram
-
-
-
r
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
Perinereis sp.
-
-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
-
-
-
-
-
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
Polycelis cornuta, Myxicola infundibulum
-
-
-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
Namalycastis sp.
Q6AW42, Q6AW43
-
-
-
r
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
Perinereis sp.
-
specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
-
not: arginine, creatine, lombricine
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
Perinereis sp.
-
-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
show the reaction diagram
Namalycastis sp.
Q6AW42, Q6AW43
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Perinereis sp.
-
29% of Mg2+-activation
Ca2+
-
slightly
Mg2+
Perinereis sp.
-
5 mM, activation
Mg2+
Namalycastis sp.
Q6AW42, Q6AW43
essential for activation
Mn2+
Perinereis sp.
-
5 mM, activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3-guanidinopropionate
Perinereis sp.
-
slight
4-Guanidinobutyrate
Perinereis sp.
-
slight
5,5'-dithiobis(2-nitrobenzoate)
Perinereis sp.
-
-
agmatine
Perinereis sp.
-
-
AMP
Perinereis sp.
-
-
Ca2+
Perinereis sp.
-
-
Chloroacetophenone
-
-
Creatine
Perinereis sp.
-
slight
guanidine
Perinereis sp.
-
slight
Hg2+
Perinereis sp.
-
-
iodoacetamide
Perinereis sp.
-
-
iodoacetic acid
-
-
L-arginine
Perinereis sp.
-
slight
Methylguanine
Perinereis sp.
-
slight
-
N-bromosuccinimide
Perinereis sp.
-
-
N-ethylmaleimide
-
-
NH2OH
-
-
p-chloromercuribenzoate
Perinereis sp.
-
-
p-chloromercuribenzoate
-
-
phenylhydrazine
-
inhibits phosphorylation of ADP
Tauroguanine
Perinereis sp.
-
slight
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10
ADP
-
-
0.8
ATP
Perinereis sp.
-
pH 8.1, 25C
0.89
ATP
Namalycastis sp.
Q6AW42, Q6AW43
native GK
1.02
ATP
Namalycastis sp.
Q6AW42, Q6AW43
recombinant GK beta
3.3
ATP
-
-
3.38
glycocyamine
Namalycastis sp.
Q6AW42, Q6AW43
recombinant GK beta
4.7
glycocyamine
Namalycastis sp.
Q6AW42, Q6AW43
native GK
0.33
Guanidinoacetate
-
-
4.1
Guanidinoacetate
Perinereis sp.
-
pH 8.1, 25C
6.8
phosphoguanidinoacetate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
24
glycocyamine
Namalycastis sp.
Q6AW42, Q6AW43
recombinant GK beta
25.3
glycocyamine
Namalycastis sp.
Q6AW42, Q6AW43
native GK
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45.3
Perinereis sp.
-
pH 8.1, 25C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8
-
phosphorylation of ADP
8.1
Perinereis sp.
-
phosphorylation of guanidinoacetate
8.9
-
phosphorylation of guanidinoacetate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 9
Perinereis sp.
-
less than 50% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
22
-
phosphorylation of guanidinoacetate
35
Perinereis sp.
-
phosphorylation of guanidinoacetate
40
-
optimum above, phosphorylation of ADP
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
16 - 32
-
less than 50% of maximal activity above and below, phosphorylation of guanidinoacetate
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Namalycastis sp.
Q6AW42, Q6AW43
-
Manually annotated by BRENDA team
Polycelis cornuta, Myxicola infundibulum
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Namalycastis sp.
Q6AW42, Q6AW43
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40000
Namalycastis sp.
Q6AW42, Q6AW43
SDS-PAGE
661520
79000
-
sucrose density gradient centrifugation
642365
80000
-
gel filtration
642369
82000
-
gel filtration
642365
87500
-
amino acid composition
642365
89150
-
equilibrium sedimentation centrifugation
642365
90000
Perinereis sp.
-
gel filtration
642364
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
Perinereis sp.
-
1 * 47000 + 1 * 45000, SDS-PAGE
dimer
-
1 * 42200 + 1 * 43800, SDS-PAGE
dimer
Namalycastis sp.
Q6AW42, Q6AW43
alphabeta, 2 * ca. 40000, SDS-PAGE
heterodimer
Namalycastis sp.
Q6AW42
heterodimer with two homologous polypeptide chains, alpha and beta, derived from a common pre mRNA by mutually exclusive N-terminal alternative exons
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the crystal structures of recombinant glycocyamine kinase alphabeta and glycocyamine kinase betabeta from Namalycastis sp. are determined at 2.6 A and 2.4 A resolution, respectively. In addition, the structure of the glycocyamine kinase betabeta is determined at 2.3 A resolution in complex with a transition state analog, Mg2+ ADP-NO3- glycocyamine. In both heterodimeric and homodimeric glycocyamine kinase forms, the conformations of the two N-termini are asymmetric and the asymmetry is different than that reported previously for the homodimeric creatinine kinases from several organisms
Namalycastis sp.
Q6AW42
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 9.5
Perinereis sp.
-
-
642364
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
Perinereis sp.
-
stable up to
642364
40
Perinereis sp.
-
20 min, 4% loss of activity
642364
45
Perinereis sp.
-
20 min, 28% loss of activity
642364
50
Perinereis sp.
-
inactivation
642364
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the recombinant GK alpha enzyme is quite unstable, storage on ice for 4 h caused 10% loss of activity, in contrast GK beta enzyme is stable, the instability of GK alpha is most likely due to its expression as a maltose-binding protein fusion protein
Namalycastis sp.
Q6AW42, Q6AW43
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, ammonium sulfate precipitate
-
-20C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months
Perinereis sp.
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, gel filtration on Sephacryl S-200n, and DEAE-5PW ion exchange chromatography
Namalycastis sp.
Q6AW42, Q6AW43
using Ni-NTA chromatography
Namalycastis sp.
Q6AW42
-
Perinereis sp.
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
Namalycastis sp.
Q6AW42
expression in Escherichia coli as maltose-binding protein fusion proteins
Namalycastis sp.
Q6AW42, Q6AW43