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Information on EC 2.7.2.11 - glutamate 5-kinase and Organism(s) Pseudomonas aeruginosa

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EC Tree
IUBMB Comments
In the absence of downstream enzymes, the product rapidly cyclizes to 5-oxo-L-proline and phosphate.
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This record set is specific for:
Pseudomonas aeruginosa
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
gamma-glutamyl kinase, glutamate 5-kinase, glutamate kinase, gamma-gk, glutamate-5-kinase, gamma-glutamate kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP-L-glutamate 5-phosphotransferase
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-
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ATP:gamma-L-glutamate phosphotransferase
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gamma-glutamate kinase
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gamma-glutamyl kinase
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gamma-glutamylphosphate kinase
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glutamate kinase
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GPK
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kinase (phosphorylating), glutamate
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kinase, glutamate (phosphorylating)
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:L-glutamate 5-phosphotransferase
In the absence of downstream enzymes, the product rapidly cyclizes to 5-oxo-L-proline and phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
54596-30-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-ethyl-L-glutamate
?
show the reaction diagram
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5% of the activity with L-glutamate
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-
?
ATP + 5-methyl-L-glutamate
?
show the reaction diagram
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6% of the activity with L-glutamate
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-
?
ATP + L-glutamate
ADP + L-glutamate 5-phosphate
show the reaction diagram
-
-
-
-
?
ATP + L-glutamine
?
show the reaction diagram
-
10% of the activity with L-glutamate
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-
?
GTP + L-glutamate
GDP + L-glutamate 5-phosphate
show the reaction diagram
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10% of the activity with ATP
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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required
Mn2+
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can partially replace Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-oxo-L-Pro
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12 mM, 10% inhibition
Cd2+
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0.1 mM, complete inhibition
DL-3,4-Didehydroproline
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9 mM, 50% inhibition
Hg2+
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0.1 mM, complete inhibition
iodoacetamide
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0.125 mM, 60% inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects
L-azetidine-2-carboxylic acid
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3 mM, 50% inhibition
L-methionine-DL-sulphoximine
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competitive with L-glutamate
L-Orn
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12 mM, 10% inhibition
L-Pro
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enzyme from strain PAO1: 5 mM, 50% inhibition, complete inhibition at 30 mM, noncompetitive. Strain PAO 879, a proline-auxotroph mutant lacks a proline-inhibitable gamma-glutamyl kinase
L-Thioproline
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12 mM, 10% inhibition
Mg2+
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above 20 mM
Mn2+
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above 20 mM
NEM
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0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects
PCMB
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0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12
L-glutamate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
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pH 5.5: about 80% of maximal activity, pH 8.5: about 40% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8F9K7K5_PSEAI
372
1
39906
TrEMBL
-
A0A4P0TGM1_PSEAI
128
0
13938
TrEMBL
-
A0A069Q072_PSEAI
372
1
39846
TrEMBL
-
A0A2R3IZH2_PSEAI
372
1
39876
TrEMBL
-
A0A8G4JX86_PSEAI
372
1
39876
TrEMBL
-
A0A8G3TDD6_PSEAI
372
1
39874
TrEMBL
-
A0A643J9A2_PSEAI
372
1
39816
TrEMBL
-
A0A8G7HWJ6_PSEAI
372
1
39816
TrEMBL
-
A0A8G2PG53_PSEAI
372
1
39801
TrEMBL
-
A0A0A8RKD7_PSEAI
372
1
39828
TrEMBL
-
A0A8G6VGM6_PSEAI
372
1
39900
TrEMBL
-
A0A8G2RXQ7_PSEAI
372
1
39819
TrEMBL
-
A0A8G3CXB7_PSEAI
372
1
39801
TrEMBL
-
A0A8G7MMS7_PSEAI
372
1
39774
TrEMBL
-
A0A4P0THD3_PSEAI
258
1
27209
TrEMBL
-
A0A8F9JLG7_PSEAI
372
1
39836
TrEMBL
-
A0A8G6WRT0_PSEAI
372
1
39818
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
84000
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gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40°C, 40% loss of activity within 2 months, then remains stable
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Krishna, R.V.; Leisinger, T.
Biosynthesis of proline in Pseudomonas aeruginosa. Partial purification and characterization of gamma-glutamyl kinase
Biochem. J.
181
215-222
1979
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO 1
Manually annotated by BRENDA team