Information on EC 2.7.11.24 - mitogen-activated protein kinase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
2.7.11.24
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RECOMMENDED NAME
GeneOntology No.
mitogen-activated protein kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (MAPKK-activated)
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline [6]. A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
CAS REGISTRY NUMBER
COMMENTARY hide
142243-02-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains 13-16-R1 and M-line
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain 8/1
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Manually annotated by BRENDA team
strain 8/1
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Manually annotated by BRENDA team
f. sp. pisi T8 strain
SwissProt
Manually annotated by BRENDA team
f. sp. pisi T8 strain
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Mus musculus C3H/HEN
C3H/HeN mice
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Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
synthetic construct
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Manually annotated by BRENDA team
a mycoparasitic fungus of soilborne plant pathogens, e.g. S. rolfsii and R.solani, strain IMI 304061
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + activating transcription factor 2
ADP + phosphorylated activating transcription factor 2
show the reaction diagram
ATP + AP1
ADP + phosphorylated AP1
show the reaction diagram
ATP + ATF-2
ADP + phosphorylated ATF-2
show the reaction diagram
ATP + ATF2
ADP + a phosphorylated ATF2
show the reaction diagram
-
substrate in assay, biotinylated ATF2
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ATP + ATF2
ADP + phosphorylated ATF2
show the reaction diagram
ATP + ATF2DELTA109
ADP + phosphorylated ATF2DELTA109
show the reaction diagram
-
-
-
-
?
ATP + Axl2
ADP + phospho-Axl2
show the reaction diagram
-
substrate of Hog1
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-
?
ATP + Bcl-2
ADP + phosphorylated Bcl-2
show the reaction diagram
-
-
-
-
?
ATP + c-Jun
ADP + phosphorylated c-Jun
show the reaction diagram
ATP + c-Jun activation domain
ADP + phosphorylated c-Jun activation domain
show the reaction diagram
ATP + c-Jun transcription factor
ADP + phosphorylated c-Jun transcription factor
show the reaction diagram
-
JNK phosphorylates the N-terminal transactivation domain of c-Jun transcription factor
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-
?
ATP + casein
ADP + phosphocasein
show the reaction diagram
-
substrate of Hog1
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-
?
ATP + cdc42
ADP + phosphorylated cdc42
show the reaction diagram
-
substrate of Gic2
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-
?
ATP + DNA polymerase II
ADP + phosphorylated DNA polymerase II
show the reaction diagram
-
substrate of Hog1p
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-
?
ATP + EGF receptor peptide
ADP + phosphorylated EGF receptor peptide
show the reaction diagram
-
-
-
-
?
ATP + Elk1
ADP + phosphorylated Elk1
show the reaction diagram
ATP + ELKERK
?
show the reaction diagram
synthetic construct
-
ERK1
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-
?
ATP + ERKMEK1
?
show the reaction diagram
synthetic construct
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ERK1
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?
ATP + ERKMEK2
?
show the reaction diagram
synthetic construct
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ERK1
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?
ATP + ERKSTE7
?
show the reaction diagram
synthetic construct
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ERK1
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?
ATP + ERKSub
?
show the reaction diagram
synthetic construct
-
ERK1 and p38alpha kinase
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?
ATP + Ets-1
ADP + phosphorylated Ets-1
show the reaction diagram
-
-
-
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?
ATP + FITC-Aca-Ala-Ala-Ala-Thr-Gly-Pro-Leu-Ser-Pro-Gly-Pro-Phe-Ala-NH2
ADP + phosphorylated FITC-Aca-Ala-Ala-Ala-Thr-Gly-Pro-Leu-Ser-Pro-Gly-Pro-Phe-Ala-NH2
show the reaction diagram
-
FITC-labeled ERK substrate peptide
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-
?
ATP + Gic2
ADP + phosphorylated Gic2
show the reaction diagram
-
substrate of Fus3, and of Hog1
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-
?
ATP + GST-c-Jun
ADP + phosphorylated GST-c-Jun
show the reaction diagram
-
substrate in kinase activity assay
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?
ATP + histone H1
ADP + phospho-histone H1
show the reaction diagram
-
substrate of Hog1
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-
?
ATP + Hog1D
ADP + phospho-Hog1D
show the reaction diagram
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substrate of Hog1
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?
ATP + Hot1p
ADP + phosphorylated Hot1p
show the reaction diagram
ATP + Hsl1
ADP + phospho-Hsl1
show the reaction diagram
-
substrate of Hog1
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-
?
ATP + human glucocorticoid receptor
ADP + phosphorylated human glucocorticoid receptor
show the reaction diagram
ATP + IRS-1
ADP + phosphorylated IRS-1
show the reaction diagram
-
phosphorylation of the insulin receptor substrate IRS-1 at serine 307
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?
ATP + JunD
ADP + phosphorylated JunD
show the reaction diagram
-
-
-
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?
ATP + Lin-1
ADP + phosphorylated Lin-1
show the reaction diagram
ATP + MAPK
ADP + phosphorylated MAPK
show the reaction diagram
-
-
-
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?
ATP + MAPKAP kinase-2
ADP + phosphorylated MAPKAP kinase-2
show the reaction diagram
-
-
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ATP + MAPKAP kinase-3
ATP + phosphorylated MAPKAP kinase-3
show the reaction diagram
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-
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ATP + MAPKAP-K2
ADP + phosphorylated MAPKAP-K2
show the reaction diagram
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-
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?
ATP + MAPKAP-K3
ADP + phosphorylated MAPKAP-K3
show the reaction diagram
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?
ATP + MAPKAPK2-peptide
ADP + phosphorylated MAPKAPK2-peptide
show the reaction diagram
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the peptide substrate is derived from a sequence of a mitogen-activated protein kinase activated protein kinase-2, MAPKAPK2, phopshorylation site
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?
ATP + MBP
ADP + phospho-MBP
show the reaction diagram
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substrate of Hog1
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?
ATP + MEF2
ADP + phosphorylated MEF2
show the reaction diagram
-
-
-
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?
ATP + MEK
ADP + phosphorylated MEK
show the reaction diagram
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binding to ERK requires docking domain and the kinase interaction motif
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?
ATP + Mek1
ADP + phospho-Mek1
show the reaction diagram
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substrate of Hog1
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?
ATP + MEK1ERK
?
show the reaction diagram
synthetic construct
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ERK1 and p38alpha kinase
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?
ATP + MEK2ERK
?
show the reaction diagram
synthetic construct
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ERK1 and p38alpha kinase
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?
ATP + MK2
ADP + phosphorylated MK2
show the reaction diagram
ATP + MKS1
ADP + phosphorylated MSK1
show the reaction diagram
ATP + MMP-9
ADP + phosphorylated MMP-9
show the reaction diagram
ATP + Mps1
ADP + phosphorylated Mps1
show the reaction diagram
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Mps1 phosphorylation by MAPK at S844, spindle checkpoint requires phosphorylation at S844, may create a phosphoepitope that allows Mps1 to interact with kinetochores
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?
ATP + multifunctional protein CAD
ADP + phosphorylated multifunctional protein CAD
show the reaction diagram
ATP + myelin basic protein
ADP + a phosphorylated myelin basic protein
show the reaction diagram
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substrate in kinase assay
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?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
ATP + p38
ADP + phosphorylated p38
show the reaction diagram
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-
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?
ATP + phospholipase C-gamma1
ADP + phosphorylated phospholipase C-gamma1
show the reaction diagram
ATP + protein
ADP + phosphoprotein
show the reaction diagram
ATP + protein APP
ADP + phosphorylated protein APP
show the reaction diagram
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-
-
-
?
ATP + protein ATF2
ADP + phosphorylated protein ATF2
show the reaction diagram
ATP + protein EGFRP
ADP + phosphorylated protein EGFRP
show the reaction diagram
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epidermal growth factor receptor peptide, substrate in kinase activity assay
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?
ATP + protein tyrosine kinase 2
ADP + phosphorylated protein tyrosine kinase 2
show the reaction diagram
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substrate of Hog1
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?
ATP + RAD9
ADP + phospho-RAD9
show the reaction diagram
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high activity with Fus3, low activity with Hog1
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?
ATP + RAD9p
ADP + phospho-RAD9p
show the reaction diagram
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substrate of Hog1
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?
ATP + Rck2
ADP + phosphorylated Rck2
show the reaction diagram
-
-
-
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?
ATP + Red1
ADP + phospho-Red1
show the reaction diagram
-
preferred substrate of Hog1
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?
ATP + RSK
ADP + phosphorylated RSK
show the reaction diagram
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binding to ERK requires docking domain
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?
ATP + SCRAMMMEK2
?
show the reaction diagram
synthetic construct
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ERK1
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?
ATP + Smad1
ADP + phosphorylated Smad1
show the reaction diagram
ATP + Smad3
ADP + phosphorylated Smad3
show the reaction diagram
ATP + sodium channel Na(v)1.6
ADP + phosphorylated sodium channel Na(v)1.6
show the reaction diagram
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-
-
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?
ATP + sodium channel Na(v)1.7
ADP + phosphorylated sodium channel Na(v)1.7
show the reaction diagram
-
-
-
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?
ATP + sodium channel Na(v)1.8
ADP + phosphorylated sodium channel Na(v)1.8
show the reaction diagram
-
-
-
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?
ATP + Ste50
ADP + phosphorylated Ste50
show the reaction diagram
ATP + STE7ERK
?
show the reaction diagram
synthetic construct
-
ERK1
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-
?
ATP + Swe1
ADP + phospho-Swe1
show the reaction diagram
-
substrate of Hog1
-
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?
ATP + Swi6
ADP + phospho-Swi6
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + TBP
ADP + phosphorylated TBP
show the reaction diagram
-
substrate of p38 MAPK
-
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?
ATP + transcription factor ATF2
ADP + phosphorylated transcription factor ATF2
show the reaction diagram
-
-
-
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-
ATP + transcription factor Djun
ADP + phosphorylated transcription factor Djun
show the reaction diagram
-
-
-
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ATP + transcription factor Elk-1
ADP + phosphorylated transcription factor Elk-1
show the reaction diagram
-
-
-
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-
ATP + transcription factor SAP-1
ADP + phosphorylated transcription factor SAP-1
show the reaction diagram
-
-
-
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-
ATP + Tub4p
ADP + phospho-Tub4
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + tyrosine hydroxylase
ADP + phosphorylated tyrosine hydroxylase
show the reaction diagram
ATP + WRKY25
ADP + phosphorylated WRKY25
show the reaction diagram
-
the transcription factor is an in vitro substrate of MPK4
-
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?
ATP + WRKY33
ADP + phosphorylated WRKY33
show the reaction diagram
-
the transcription factor is an in vitro substrate of MPK4
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-
?
phosphoprotein
?
show the reaction diagram
-
the MAPK is regulated in the MAPK signaling cascade by 2 mechanisms: 1. by MEK, EC 2.7.11.25, docking at the allosteric ED domain or the CD domain of MAPKs, or 2. by MKK7, MLK, JNK or MKP-7 docking at the scaffolding protein JIP in the JNK signaling pathway
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + activating transcription factor 2
ADP + phosphorylated activating transcription factor 2
show the reaction diagram
-
ATF2
-
-
?
ATP + AP1
ADP + phosphorylated AP1
show the reaction diagram
-
substrate of ERK1/2, ERK access to the substrate is regulated by the all-trans retinoic acid receptor, RAR
-
-
?
ATP + c-Jun
ADP + phosphorylated c-Jun
show the reaction diagram
ATP + DNA polymerase II
ADP + phosphorylated DNA polymerase II
show the reaction diagram
-
substrate of Hog1p
-
-
?
ATP + Elk1
ADP + phosphorylated Elk1
show the reaction diagram
ATP + Hot1p
ADP + phosphorylated Hot1p
show the reaction diagram
-
substrate of Hog1p, phosphorylation of Hot1p is not required for Hot1p-mediated gene expression
-
-
?
ATP + human glucocorticoid receptor
ADP + phosphorylated human glucocorticoid receptor
show the reaction diagram
-
specific phosphorylation at Ser211 by p38 MAPK, p38 MAPK is a mediator in glucocorticoid-induced apoptosis of lymphoid cells, interaction of MAPK and glucocorticoid pathways, overview
-
-
?
ATP + Lin-1
ADP + phosphorylated Lin-1
show the reaction diagram
P53778
substrate of ERK2, negative regulation of Lin-1
-
-
?
ATP + MAPKAP-K2
ADP + phosphorylated MAPKAP-K2
show the reaction diagram
-
-
-
-
?
ATP + MAPKAP-K3
ADP + phosphorylated MAPKAP-K3
show the reaction diagram
-
-
-
-
?
ATP + MEF2
ADP + phosphorylated MEF2
show the reaction diagram
-
-
-
-
?
ATP + MKS1
ADP + phosphorylated MSK1
show the reaction diagram
-
MPK4 acts as a regulator of pathogen defense responses and is required for repression of salicylic acid-dependent resistance and for activation of jasmonate-dependent defense gene expression via MSK1, which interacts with the transcription factors WRKY25 and WRKY33
-
-
?
ATP + MMP-9
ADP + phosphorylated MMP-9
show the reaction diagram
-
activity of p38 MAP kinase, TNF-alpha stimulates MMP-9 expression via the p38 MAP kinase signaling pathway in 5637 cells, and p38 MAP kinase-mediated MMP-9 gene regulation in response to TNF-alpha is involved in the NF-kappaB response element in 5637 cells, regulation, overview
-
-
?
ATP + multifunctional protein CAD
ADP + phosphorylated multifunctional protein CAD
show the reaction diagram
ATP + phospholipase C-gamma1
ADP + phosphorylated phospholipase C-gamma1
show the reaction diagram
P63086
the reaction is performed by activated phosphorylated ERK2, phosphorylation inhibits phospholipase C-gamma1
-
-
?
ATP + Smad1
ADP + phosphorylated Smad1
show the reaction diagram
-
the MAP kinase antagonizes Smad1 in signaling during development of axis and neural specification, Smad1 is involved in dorsal-ventral patterning in embryos
-
-
?
ATP + Smad3
ADP + phosphorylated Smad3
show the reaction diagram
-
substrate of MAPKs, e.g. ERK2
-
-
?
ATP + Ste50
ADP + phosphorylated Ste50
show the reaction diagram
ATP + TBP
ADP + phosphorylated TBP
show the reaction diagram
-
substrate of p38 MAPK
-
-
?
ATP + tyrosine hydroxylase
ADP + phosphorylated tyrosine hydroxylase
show the reaction diagram
-
phosphorylation of tyrosine hydroxylase at Ser8 and Ser31 by ERK1 and ERK2 is involved in regulation of catecholamine biosynthesis
-
-
?
additional information
?
-