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Information on EC 2.7.11.23 - [RNA-polymerase]-subunit kinase and Organism(s) Drosophila melanogaster

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EC Tree
IUBMB Comments
The enzyme appears to be distinct from other protein kinases. It brings about multiple phosphorylations of the unique C-terminal repeat domain of the largest subunit of eukaryotic DNA-directed RNA polymerase (EC 2.7.7.6). The enzyme does not phosphorylate casein, phosvitin or histone.
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This record set is specific for:
Drosophila melanogaster
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Synonyms
dna-pk, tfiih, p-tefb, dna-dependent protein kinase, positive transcription elongation factor b, ctd kinase, kin28, srb10, ctdk-i, tfiih kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-terminal repeat domain kinase
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CTD kinase
kinase, ribonucleate nucleotidyltransferase II C-terminal domain (phosphorylating)
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kinase, ribonucleate nucleotidyltransferase isozyme II IIa subunit (phosphorylating)
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Pol II CTD Ser2 kinase
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Pol II kinase
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positive transcription elongation factor b
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RNA polymerase II carboxyl-terminal domain Ser2 kinase
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RNA polymerase II CTD kinase
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RNA polymerase II kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:[DNA-directed RNA polymerase] phosphotransferase
The enzyme appears to be distinct from other protein kinases. It brings about multiple phosphorylations of the unique C-terminal repeat domain of the largest subunit of eukaryotic DNA-directed RNA polymerase (EC 2.7.7.6). The enzyme does not phosphorylate casein, phosvitin or histone.
CAS REGISTRY NUMBER
COMMENTARY hide
122097-00-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + RNA polymerase II
?
show the reaction diagram
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phosphorylation on C-terminal domain
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-
?
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
show the reaction diagram
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
show the reaction diagram
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
show the reaction diagram
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Positive transcription elongation factor b is the major metazoan RNA polymerase II carboxyl-terminal domain Ser2 kinase, P-TEFb is critical for the maturation of RNA PolII into productive elongation in vivo, overview
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-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
show the reaction diagram
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
cyclin K is associated with purified dCDK12, implicating it as the cyclin subunit of this CTD kinase
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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enzyme inhibition affects the binding of Hsp70 to RNA polymerase II, overview
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
distribution of dCDK12 and hyperphosphorylated RNAPII (Pol II0) on Dm polytene chromosomes by fluorescence microscopy. The dCDK12 kinase appears to be closely associated with Pol II0, not only at loci expressed normally during development, but also at heat-shock loci
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the metazoan orthologue of Saccharomyces cerevisiae Ctk1 in Drosophila melanogaster is dCDK12
malfunction
metabolism
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDK1_DROME
297
0
34439
Swiss-Prot
other Location (Reliability: 1)
CDK8_DROME
454
0
53682
Swiss-Prot
other Location (Reliability: 1)
CDK2_DROME
314
0
35888
Swiss-Prot
other Location (Reliability: 1)
CDK12_DROME
1157
0
128333
Swiss-Prot
other Location (Reliability: 2)
Q24216_DROME
353
0
39673
TrEMBL
other Location (Reliability: 1)
M9PG50_DROME
1157
0
128333
TrEMBL
other Location (Reliability: 2)
O17432_DROME
404
0
46787
TrEMBL
Mitochondrion (Reliability: 5)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
cyclin K is associated with purified dCDK12, implicating it as the cyclin subunit of this CTD kinase
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged chimeric mutant Ctk1/12 kinase from Saccharomyces cerevisiae strain FY23 by cation exchange chromatography and nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
dCDK12, recombinant expression of the His-tagged chimeric mutant Ctk1/12 kinase in Saccharomyces cerevisiae strain FY23
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Palancade, B.; Bensaude, O.
Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation
Eur. J. Biochem.
270
3859-3870
2003
Saccharomyces cerevisiae, Drosophila melanogaster, Homo sapiens, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Hirose, Y.; Ohkuma, Y.
Phosphorylation of the C-terminal domain of RNA polymerase II plays central roles in the integrated events of eucaryotic gene expression
J. Biochem.
141
601-608
2007
Saccharomyces cerevisiae, Drosophila melanogaster, Homo sapiens, Xenopus laevis
Manually annotated by BRENDA team
Ni, Z.; Saunders, A.; Fuda, N.J.; Yao, J.; Suarez, J.R.; Webb, W.W.; Lis, J.T.
P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo
Mol. Cell. Biol.
28
1161-1170
2008
Drosophila melanogaster
Manually annotated by BRENDA team
Heidemann, M.; Hintermair, C.; Vo, K.; Eick, D.
Dynamic phosphorylation patterns of RNA polymerase II CTD during transcription
Biochim. Biophys. Acta
1829
55-62
2013
Schizosaccharomyces pombe (O14098), Schizosaccharomyces pombe (Q12126), Schizosaccharomyces pombe (Q96WV9), Saccharomyces cerevisiae (P06242), Saccharomyces cerevisiae (P23293), Saccharomyces cerevisiae (P39073), Saccharomyces cerevisiae (Q03957), Homo sapiens (P49336), Homo sapiens (P50613), Homo sapiens (P50750), Homo sapiens (Q9H4B4), Drosophila melanogaster (Q24216), Drosophila melanogaster (Q9VT57), Schizosaccharomyces pombe ATCC 24843 (O14098), Schizosaccharomyces pombe ATCC 24843 (Q12126), Schizosaccharomyces pombe ATCC 24843 (Q96WV9)
Manually annotated by BRENDA team
Bartkowiak, B.; Liu, P.; Phatnani, H.P.; Fuda, N.J.; Cooper, J.J.; Price, D.H.; Adelman, K.; Lis, J.T.; Greenleaf, A.L.
CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1
Genes Dev.
24
2303-2316
2010
Homo sapiens (Q14004), Homo sapiens (Q9NYV4), Homo sapiens, Drosophila melanogaster (Q9VP22)
Manually annotated by BRENDA team
Li, X.; Chatterjee, N.; Spirohn, K.; Boutros, M.; Bohmann, D.
Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes
Sci. Rep.
6
21455
2016
Drosophila melanogaster
Manually annotated by BRENDA team