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ATP + Lys-Lys-Arg-Ala-Ala-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ala
ADP + ?
-
-
-
-
?
ATP + Lys-Lys-Arg-Pro-Gln-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ser
ADP + ?
-
-
-
-
?
ATP + myosin IIB
ADP + phosphorylated myosin IIB
-
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
ATP + myosin light chain
ADP + phosphorylated myosin light chain
ATP + telokin
ADP + ?
-
telokin may modulate enzyme activity in vivo
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
ATP + [non-muscle heavy meromyosin IIB]
ADP + [non-muscle heavy meromyosin IIB] phosphate
-
-
-
?
ATP + [non-muscle regulatory light chain]
ADP + [non-muscle regulatory light chain] phosphate
-
-
-
?
ATP + [smooth muscle heavy meromyosin]
ADP + [smooth muscle heavy meromyosin] phosphate
-
-
-
?
ATP + [smooth muscle regulatory light chain]
ADP + [smooth muscle regulatory light chain] phosphate
-
-
-
?
additional information
?
-
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrates are myosin heavy chain and phosvitin
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of skeletal muscle
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of non-muscle myosin
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
not specific for 22 kDa and 15 kDa light chain
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is casein
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is troponin
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
1 mol phosphate per mol light chain in skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is phosphorylase kinase
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
acceptor substrates are myosin light chains of smooth muscle
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is histone V-S
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
no substrate is phosphorylase b
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
kinase from skeletal muscle with broader specificity than smooth muscle kinase
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
involved in muscle contractility and motility of non-muscle cells
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
event in initiation of smooth-muscle contraction
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
inhibition of actin-myosin ineraction
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, myosin light chain phosphorylation is increased under these conditions, suggesting that contraction would be potentiated by ablation of AMPK
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
the enzyme has effects on the actin-myosin interaction in vitro, regulation of actin-myosin interaction by MLCK through its non-kinase activity, overview
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
-
20 kDa regulatory light chain of smooth muscle
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
-
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
-
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
-
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
non-muscle myosin II
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
non-muscle myosin II and preferred substrate smooth muscle myosin II
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
phosphorylation of myosin on Ser19 of the regulatory light chain. Non-muscle myosin II regulatory light chain and preferred substrate smooth muscle myosin II regulatory light chain
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
smooth muscle myosin regulatory light chain phosphorylation at Ser19
-
-
?
additional information
?
-
-
skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate
-
-
?
additional information
?
-
-
the enzyme is a potential cytoskeleton integrator through its unique N-terminal domain, the enzyme binds tubulin and actin, and is involved in microtubule/microfilament association
-
-
?
additional information
?
-
-
the enzyme is a key regulator of various forms of cell motility involving actin and myosin II
-
-
?
additional information
?
-
-
actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview. MLCK phosphorylated at Ser828 stimulates the ATPase activity of unphosphorylated myosin more effectively than the control MLCK
-
-
?
additional information
?
-
MLCK is a substrate of AMP-activated protein kinase, AMPK
-
-
?
additional information
?
-
-
MLCK is a substrate of AMP-activated protein kinase, AMPK
-
-
?
additional information
?
-
-
the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities
-
-
?
additional information
?
-
non-muscle myosin and smooth muscle myosin are the only known substrates of MLCK in vivo
-
-
?
additional information
?
-
MLCK has a 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar. Myosin light chain kinase has a 1.6fold and 1.5fold higher specificity (kcat/Km) for smooth versus non-muscle-free regulatory light chain and heavy meromyosin, respectively, suggesting that differences in specificity are dictated by regulatory light chain sequences. Three residues, Glu70, Gly71, and Ser74, are found to be surface exposed in the N-terminal half of the regulatory light chain, consistent with their importance in substrate recognition. Substrate recognition and specificity, overview
-
-
?
additional information
?
-
static substrate binding structure of streptavidin-tagged enzyme, overview
-
-
?
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Collinge, M.; Matrisian, P.E.; Zimmer, W.E.; Shattuck, R.L.; Lukas, T.J.; Van Eldik, L.J.; Watterson, D.M.
Structure and expression of a calcium-binding protein gene contained within a calmodulin-regulated protein kinase gene
Mol. Cell. Biol.
12
2359-2371
1992
Gallus gallus (P11799), Gallus gallus
brenda
Guerriero, V.Jr.; Russo, M.A.; Olson, N.J.; Putkey, J.A.; Means, A.R.
Domain organization of chicken gizzard myosin light chain kinase deduced from a cloned cDNA
Biochemistry
25
8372-8381
1986
Gallus gallus (P11799), Gallus gallus
brenda
Olson, N.J.; Pearson, R.B.; Needleman, D.S.; Hurwitz, M.Y.; Kemp, B.E.; Means, A.R.
Regulatory and structural motifs of chicken gizzard myosin light chain kinase
Proc. Natl. Acad. Sci. USA
87
2284-2288
1990
Gallus gallus (P11799), Gallus gallus
brenda
Shoemaker, M.O.; Lau, W.; Shattuck, R.L.; Kwiatkowski, A.P.; Matrisian, P.E.; Guerra-Santos, L.; Wilson, E.; Lukas, T.J.; Van Eldik, L.J.; Watterson, D.M.
Use of DNA sequence and mutant analyses and antisense oligodeoxynucleotides to examine the molecular basis of nonmuscle myosin light chain kinase autoinhibition, calmodulin recognition, and activity
J. Cell Biol.
111
1107-1125
1990
Gallus gallus (P11799), Gallus gallus
brenda
Watterson, D.M.; Collinge, M.; Lukas, T.J.; Van Eldik, L.J.; Birukov, K.G.; Stepanova, O.V.; Shirinsky, V.P.
Multiple gene products are produced from a novel protein kinase transcription region
FEBS Lett.
373
217-220
1995
Gallus gallus (P11799)
brenda
Yoshikai, S.; Ikebe, M.
Molecular cloning of the chicken gizzard telokin gene and cDNA
Arch. Biochem. Biophys.
299
242-247
1992
Gallus gallus (P11799), Gallus gallus
brenda
Gallagher, P.J.; Herring, B.P.; Griffin, S.A.; Stull, J.T.
Molecular characterization of a mammalian smooth muscle myosin light chain kinase [published erratum appears in J Biol Chem 1992 May 5;267(13):9450]
J. Biol. Chem.
266
23936-23944
1991
Bos taurus, Gallus gallus, Oryctolagus cuniculus, Oryctolagus cuniculus (P29294), Rattus norvegicus
brenda
Walsh, M.P.; Hinkins, S.; Flink, I.L.; Hartshorne, D.J.
Bovine stomach myosin light chain kinase: purification, characterization, and comparison with the turkey gizzard enzyme
Biochemistry
21
6890-6896
1982
Bos taurus, Gallus gallus, Meleagris gallopavo
brenda
Yamazaki, K.; Itoh, K.; Sobue, K.; Mori, T.; Shibata, N.
Purification of caldesmon and myosin light chain (MLC) kinase from arterial smooth muscle: comparisons with gizzard caldesmon and MLC kinase
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101
1-9
1987
Bos taurus, Gallus gallus
brenda
Jinsart, W.; Ternai, B.; Polya, G.M.
Inhibition of myosin light chain kinase, cAMP-dependent protein kinase, protein kinase C and of plant Ca(2+)-dependent protein kinase by anthraquinones
Biol. Chem. Hoppe-Seyler
373
903-910
1992
Gallus gallus
brenda
Nunnally, M.H.; Rybicki, S.B.; Stull, J.T.
Characterization of chicken skeletal muscle myosin light chain kinase. Evidence for muscle-specific isozymes
J. Biol. Chem.
260
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1985
Gallus gallus, Oryctolagus cuniculus
brenda
Leachman, S.A.; Gallagher, P.J.; Herring, B.P.; McPhaul, M.J.; Stull, J.T.
Biochemical properties of chimeric skeletal and smooth muscle myosin light chain kinases
J. Biol. Chem.
267
4930-4938
1992
Gallus gallus
brenda
Bailin, G.
Structure and function of a calmodulin-dependent smooth muscle myosin light chain kinase
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40
1185-1188
1984
Bos taurus, Gallus gallus, Oryctolagus cuniculus, Meleagris gallopavo
brenda
Jinsart, W.; Ternai, B.; Polya, G.M.
Inhibition of wheat embryo calcium-dependent protein kinase and avian myosin light chain kinase by flavonoids and related compounds
Biol. Chem. Hoppe-Seyler
372
819-827
1991
Gallus gallus
brenda
Nakanishi, S.; Kakita, S.; Takahashi, I.; Kawahara, K.; Tsukada, E.; Sano, T.; Yamada, K.; Yoshida, M.; Kase, H.; Matsuda, Y.; Hashimoto, Y.; Nonomura, Y.
Wortmannin, a microbial product inhibitor of myosin light chain kinase
J. Biol. Chem.
267
2157-2163
1992
Gallus gallus
brenda
Nakanishi, S.; Ando, K.; Kawamoto, I.; Matsuda, Y.
MS-347a, a new inhibitor of myosin light chain kinase from Aspergillus sp. KY52178
J. Antibiot.
46
1775-1781
1989
Gallus gallus
-
brenda
Jinsart, W.; Ternai, B.; Polya, G.M.
Inhibition and activation of wheat embryo calcium-dependent protein kinase and inhibition of avian myosin light chain kinase by long chain aliphatic amphiphiles
Plant Sci.
78
165-175
1991
Gallus gallus
-
brenda
Stull, J.T.; Nunnally, M.H.; Michnoff, C.H in
Calmoduline-dependent protein kinases
The Enzymes, 3rd. Ed. (Boyer, P. D. , Krebs, E. G. , eds. )
17
113-166
1986
Bos taurus, Canis lupus familiaris, Cavia porcellus, Gallus gallus, Homo sapiens, Meleagris gallopavo, Rattus norvegicus
-
brenda
Fujita, K.; Ye, L.H.; Sato, M.; Okagaki, T.; Nagamachi, Y.; Kohama, K.
Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin
Mol. Cell. Biochem.
190
85-90
1999
Gallus gallus
brenda
Okagaki, T.; Ye, L.H.; Samizo, K.; Tanaka, T.; Kohama, K.
Inhibitory effect of the catalytic domain of myosin light chain kinase on actin-myosin interaction: insight into the mode of inhibition
J. Biochem.
125
1055-1060
1999
Gallus gallus
brenda
Okagaki, T.; Hayakawa, K.; Samizo, K.; Kohama, K.
Inhibition of the ATP-dependent interaction of actin and myosin by the catalytic domain of the myosin light chain kinase of smooth muscle: possible involvement in smooth muscle relaxation
J. Biochem.
125
619-626
1999
Gallus gallus
brenda
Hayakawa, K.; Okagaki, T.; Ye, L.H.; Samizo, K.; Higashi-Fujime, S.; Takagi, T.; Kohama, K.
Characterization of the myosin light chain kinase from smooth muscle as an actin-binding protein that assembles actin filaments in vitro
Biochim. Biophys. Acta
1450
12-24
1999
Gallus gallus
brenda
Sobieszek, A.; Andruchov, O.Y.; Nieznanski, K.
Kinase-related protein (telokin) is phosphorylated by smooth-muscle myosin light-chain kinase and modulates the kinase activity
Biochem. J.
328
425-430
1997
Gallus gallus
-
brenda
Edwards, R.A.; Walsh, M.P.; Sutherland, C.; Vogel, H.J.
Activation of calcineurin and smooth muscle myosin light chain kinase by Met-to-Leu mutants of calmodulin
Biochem. J.
331
149-152
1998
Gallus gallus
-
brenda
Sobieszek, A.; Borkowski, J.; Babiychuk, V.S.
Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex
J. Biol. Chem.
272
7034-7041
1997
Gallus gallus
brenda
Van Lierop, J.E.; Wilson, D.P.; Davis, J.P.; Tikunova, S.; Sutherland, C.; Walsh, M.P.; Johnson, J.D.
Activation of smooth muscle myosin light chain kinase by calmodulin. Role of Lys30 and Gly40
J. Biol. Chem.
277
6550-6558
2002
Gallus gallus, Rattus norvegicus
brenda
Smith, L.; Parizi-Robinson, M.; Zhu, M.S.; Zhi, G.; Fukui, R.; Kamm, K.E.; Stull, J.T.
Properties of long myosin light chain kinase binding to F-actin in vitro and in vivo
J. Biol. Chem.
277
35597-35604
2002
Gallus gallus
brenda
Kudryashov, D.S.; Stepanova, O.V.; Vilitkevich, E.L.; Nikonenko, T.A.; Nadezhdina, E.S.; Shanina, N.A.; Lukas, T.J.; Van Eldik, L.J.; Watterson, D.M.; Shirinsky, V.P.
Myosin light chain kinase (210 kDa) is a potential cytoskeleton integrator through its unique N-terminal domain
Exp. Cell Res.
298
407-417
2004
Gallus gallus
brenda
Dudnakova, T.V.; Stepanova, O.V.; Dergilev, K.V.; Chadin, A.V.; Shekhonin, B.V.; Watterson, D.M.; Shirinsky, V.P.
Myosin light chain kinase colocalizes with nonmuscle myosin IIB in myofibril precursors and sarcomeric Z-lines of cardiomyocytes
Cell Motil. Cytoskeleton
63
375-383
2006
Gallus gallus, Homo sapiens
brenda
Nakamura, A.; Xie, C.; Zhang, Y.; Gao, Y.; Wang, H.H.; Ye, L.H.; Kishi, H.; Okagaki, T.; Yoshiyama, S.; Hayakawa, K.; Ishikawa, R.; Kohama, K.
Role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, a review dedicated to Dr. Setsuro Ebashi
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369
135-143
2008
Bos taurus, Gallus gallus
brenda
Horman, S.; Morel, N.; Vertommen, D.; Hussain, N.; Neumann, D.; Beauloye, C.; El Najjar, N.; Forcet, C.; Viollet, B.; Walsh, M.P.; Hue, L.; Rider, M.H.
AMP-activated protein kinase phosphorylates and desensitizes smooth muscle myosin light chain kinase
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283
18505-18512
2008
Mus musculus, Gallus gallus (P11799), Gallus gallus, Rattus norvegicus (P20689)
brenda
Xie, C.; Zhang, Y.; Wang, H.H.; Matsumoto, A.; Nakamura, A.; Ishikawa, R.; Yoshiyama, S.; Hayakawa, K.; Kohama, K.; Gao, Y.
Calcium regulation of non-kinase and kinase activities of recombinant myosin light-chain kinase and its mutants
IUBMB Life
61
1092-1098
2009
Gallus gallus, Bos taurus (Q28824)
brenda
Vilitkevich, E.L.; Khapchaev, A.Y.; Kudryashov, D.S.; Nikashin, A.V.; Schavocky, J.P.; Lukas, T.J.; Watterson, D.M.; Shirinsky, V.P.
Phosphorylation regulates interaction of 210-kDa myosin light chain kinase N-terminal domain with actin cytoskeleton
Biochemistry (Moscow)
80
1288-1297
2015
Gallus gallus (P11799)
brenda
Hong, F.; Facemyer, K.C.; Carter, M.S.; Jackson, d.e.l..R.; Haldeman, B.D.; Ruana, N.; Sutherland, C.; Walsh, M.P.; Cremo, C.R.; Baker, J.E.
Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system
Biochemistry
52
8489-8500
2013
Gallus gallus (P11799)
brenda
Alcala, D.B.; Haldeman, B.D.; Brizendine, R.K.; Krenc, A.K.; Baker, J.E.; Rock, R.S.; Cremo, C.R.
Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates
Cell Biochem. Funct.
34
469-474
2016
Gallus gallus (P11799)
brenda