Information on EC 2.7.1.74 - deoxycytidine kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.1.74
-
RECOMMENDED NAME
GeneOntology No.
deoxycytidine kinase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
cytosine arabinoside can act as acceptor, all natural nucleoside triphosphates except dCTP can act as donors
-
-
-
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
mechanism
-
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
mechanism; ordered A-B random B-Q reaction sequence
-
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
random bi-bi mechanism
-
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
mechanism
-
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
active site structure, substrate binding structure simulation and analysis
-
NTP + deoxycytidine = NDP + dCMP
show the reaction diagram
substrate-enzyme interactions, bi bi random reaction mechanism with ATP, but ordered with UTP binding before the acceptor substrate, substrate binding site structures
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
purine deoxyribonucleosides salvage
-
-
pyrimidine deoxyribonucleosides salvage
-
-
purine metabolism
-
-
Purine metabolism
-
-
Pyrimidine metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
NTP:deoxycytidine 5'-phosphotransferase
Cytosine arabinoside can act as acceptor; all natural nucleoside triphosphates (except dCTP) can act as donors.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2'-deoxycytidine kinase
-
-
-
-
ara-C kinase
-
-
-
-
arabinofuranosylcytosine kinase
-
-
-
-
dC kinase
-
-
dC kinase
Bacillus megaterium KM
-
-
-
dCK
-
-
-
-
dCK
Mus musculus C57BL/6J
-
-
-
deoxycytidine kinase
-
-
deoxycytidine-cytidine kinase
-
-
-
-
dNTP:deoxycytidine 5'-phosphotransferase
-
-
kinase, deoxycytidine (phosphorylating)
-
-
-
-
NTP:deoxycytidine 5'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9039-45-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
owl monkey
-
-
Manually annotated by BRENDA team
strain KM
-
-
Manually annotated by BRENDA team
Bacillus megaterium KM
strain KM
-
-
Manually annotated by BRENDA team
; patients with B-cell chronic lymphocytic leukaemia
-
-
Manually annotated by BRENDA team
B-CLL patients
-
-
Manually annotated by BRENDA team
differences in mRNA levels in African and European individuals detected
-
-
Manually annotated by BRENDA team
patients suffering from pancreatic cancer, survival of patients is found to be correlated to the detected enzyme level
-
-
Manually annotated by BRENDA team
patients with pancreatic cancer
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Ada-/- mice
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
dCK inhibition reduces the induction of apoptosis in thymocytes
malfunction
-
inhibition of the enzyme results in diminished dAdomediated apoptosis in the lungs
malfunction
-
inhibition of the enzyme results in diminished deoxyadenosine-mediated apoptosis in the lungs
malfunction
-
mechanisms of cell killing by activated bromovinyl-deoxyuridine in Jurkat cells expressing thymidine-activating deoxycytidine kinase, cell fate control gene therapy strategy, overview. Tumor formation is repressed by dCK-based cell fate control gene therapy strategy in vivo in a xenogeneic murine model and thymidine-activating dCK mutant increased survival in a stringent model of T cell leukemia
malfunction
-
deoxxycytidine kinase inhibition causes gemcitabine resistance, overview
malfunction
-
dCK inactivation selectively and profoundly affects T and B cell development, severe impact of dCK inactivation on lymphopoiesis. Inactivation of dCK induces a partial block in B cell development
metabolism
-
dCK plays an important role in the salvage pathway of nucleoside metabolism
metabolism
-
deoxycytidine kinase is a rate-limiting enzyme in the deoxyribonucleoside salvage pathway
metabolism
-
2',2'-difluorodeoxycytidine, i.e. gemcitabine, is phosphorylated intracellularly via multiple steps, and the triphosphate inhibits DNA synthesis by interfering with incorporation of endogenous dCTP into DNA, deocycytidine kinase catalyzes the first phosphorylation step, overview
metabolism
-
dCK function in the context of cytosolic deoxycytidine metabolism, model, overview
physiological function
-
cytosolic and mitochondrial deoxynucleoside kinases, as well as 5'-deoxynucleotidases, control intracellular and intramitochondrial phosphorylation of natural nucleotides and nucleoside analogs used in antiviral and cancer chemotherapy, overview
physiological function
-
phosphorylation of Ser74 might be a mechanism for regulating the specificity of the kinase to favor deoxycytidine
physiological function
-
deoxycytidine kinase modulates the impact of the ABC transporter ABCG2 on clofarabine cytotoxicity in Saos-2 cells and OCI-AML3 cells, ABCG2 primarily effluxes clofarabine, but not chlorfarabine-monophosphate, overview
physiological function
-
deoxycitidine kinase is a major enzyme for deoxyadenosine phosphorylation. Hypoxia-induced deoxycytidine kinase expression contributes to apoptosis in chronic lung disease, hypoxia is a regulator of the enzyme
physiological function
-
deoxycitidine kinase is a major enzyme for deoxyadenosine phosphorylation. Hypoxia-induced deoxycytidine kinase expression contributes to apoptosis in chronic lung disease, hypoxia is a regulator of the enzyme. Activating the dAdo-DCK-dATP pathway directly results in increased apoptosis in the lungs of mice with air-space enlargement, the dAdo-DCK pathway is activated in the lungs of Ada-/- mice, overview
physiological function
-
deoxycytidine kinase (dCK) is a rate limiting enzyme critical for phosphorylation of endogenous deoxynucleosides for DNA synthesis and exogenous nucleoside analogues for anticancer and antiviral drug actions. The enzyme is activated in response to DNA damage. Deoxycytidine kinase regulates the G2/M checkpoint through interaction with cyclin-dependent kinase 1 in response to DNA damagereqquiring its phosphorylation at Ser74, overview
physiological function
-
deoxycytidine kinase is a key enzyme in the salvage of deoxyribonucleosides and in the activation of several anticancer and antiviral nucleoside analogues
physiological function
-
deoxycytidine kinase is a rate-limiting enzyme in deoxyribonucleoside salvage, a metabolic pathway that recycles products of DNA degradation. The enzyme phosphorylates and therefore activates nucleoside analogue prodrugs frequently used in cancer, autoimmunity, and viral infections. Normal lymphocyte development critically requires the deoxyribonucleoside salvage pathway
metabolism
Mus musculus C57BL/6J
-
deoxycytidine kinase is a rate-limiting enzyme in the deoxyribonucleoside salvage pathway
-
additional information
-
deoxycytidine kinase is a key enzyme in the activation of several therapeutic nucleoside analogues
additional information
-
structure-function analysis and substrate specificity, overview
additional information
-
structure-function analysis, comparison of wild-type enzyme and mutant S74E
additional information
-
biodistribution of cytosine derivative substrates in mouse healthy and malignant tissues, overview
additional information
Mus musculus C57BL/6J
-
biodistribution of cytosine derivative substrates in mouse healthy and malignant tissues, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',2'-difluoro-2'-deoxyguanosine + ATP
ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
2'-fluoro-2'-deoxyarabinosylcytidine + ATP
ADP + 2'-fluoro-2'-deoxyarabinosylcytidine 5'-phosphate
show the reaction diagram
-
-
-
?
2'-fluoro-2'-deoxycytidine + ATP
ADP + 2'-fluoro-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
2'-O-methyl-cytidine + ATP
ADP + 2'-O-methyl-cytidine 5'-phosphate
show the reaction diagram
-
-
-
?
2-chloro-2'-deoxyadenosine + NTP
NDP + 2-chloro-2'-deoxy-AMP
show the reaction diagram
-
-
-
?
2-chloro-2'-deoxyadenosine + NTP
NDP + 2-chloro-2'-deoxy-AMP
show the reaction diagram
-
-
-
?
3'-O-methyl-2'-deoxycytidine + ATP
ADP + 3'-O-methyl-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
4'-thio-2'-deoxycytidine + UTP
UDP + 4'-thio-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
4'-thio-beta-D-arabinofuranosylcytosine + UTP
UDP + 4'-thio-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
-
-
?
9-(beta-D-arabinofuranosyl)-adenine + ATP
9-(beta-D-arabinofuranosyl)-adenine 5'-phosphate + ADP
show the reaction diagram
-
-
-
?
9-beta-D-arabinofuranosylguanine + NTP
NDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
i.e. lamivudine
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + ?
show the reaction diagram
-
substrate of enzyme mutant R104M/D133A, poor substrate of the wild-type enzyme
-
?
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
show the reaction diagram
-
-
-
?
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
show the reaction diagram
-
-
-
?
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
show the reaction diagram
-
-
-
?
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine monophosphate
show the reaction diagram
-
-
-
?
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine monophosphate
show the reaction diagram
-
-
-
?
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine monophosphate
show the reaction diagram
-
-
-
?
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril
ADP + ?
show the reaction diagram
-
poor substrate, 1.4% of the activity with 2'-deoxycytidine
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
-
-
-
ATP + 1-beta-D-arabinosylcytosine
ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 1-beta-D-arabinosylcytosine
ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 1-beta-D-arabinosylcytosine
ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-monophosphate
show the reaction diagram
-
i.e. gemcitabine
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine monophosphate
show the reaction diagram
-
i.e. gemcitabine, i.e. gemcitabine, transport and metabolism in vivo, overview
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
i.e. zalcitabine
-
?
ATP + 2'-deoxy-3'-thiacytidine
ADP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
involved in nucleotide synthesis, involved in DNA repair
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
best phosphate donor cytosolic isozyme II and mitochondrial isozyme
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
best phosphate donor
-
?
ATP + 2'-deoxycytidine
ADP + 5'-dCMP
show the reaction diagram
-
in the presence of DTT less effective than dUTP, UTP, dTTP or GTP, without DTT only less effective than dUTP
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
ATP + 2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
ADP + ?
show the reaction diagram
-
nucleoside mimic, 14.6% of the activity with 2'-deoxycytidine
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
i.e. cladribine
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
ATP + 5-(3-pyridyl)-2'-deoxycytidine
ADP + 5-(3-pyridyl)-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
cytosolic enzyme, poor substrate
-
?
ATP + 5-(4-pyridyl)-2'-deoxycytidine
ADP + 5-(4-pyridyl)-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
cytosolic enzyme, poor substrate
-
?
ATP + 5-bromodeoxycytidine
ADP + 5-bromo-dCMP
show the reaction diagram
-
-
-
?
ATP + 5-iododeoxycytidine
ADP + 5-iodo-dCMP
show the reaction diagram
-
-
-
?
ATP + 5-methyldeoxycytidine
ADP + 5-methyl-dCMP
show the reaction diagram
-
-
-
?
ATP + 5-propynyldeoxycytidine
ADP + 5-propynyl-dCMP
show the reaction diagram
-
-
-
?
ATP + adenosine
ADP + adenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + arabinosyl adenine
ADP + arabinosyl adenine 5'-phosphate
show the reaction diagram
-
-
?
ATP + arabinosyl cytosine
ADP + arabinosyl cytosine 5'-phosphate
show the reaction diagram
-
i.e. cytosar
-
?
ATP + arabinosyl cytosine
ADP + arabinosyl cytosine 5'-phosphate
show the reaction diagram
i.e. cytosar
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + cladribine
ADP + cladribine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + cladribine
ADP + cladribine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + cladribine
ADP + phospho-cladribine
show the reaction diagram
-
-
-
?
ATP + clofarabine
ADP + phospho-clofarabine
show the reaction diagram
-
-
-
?
ATP + clofarabine
ADP + phospho-clofarabine
show the reaction diagram
-
-
-
?
ATP + clofarabine
ADP + phospho-clofarabine
show the reaction diagram
-
-
-
?
ATP + clofarabine
ADP + phospho-clofarabine
show the reaction diagram
Homo sapiens, Mus musculus C57BL/6J
-
-
-
?
ATP + cytarabine
ADP + cytarabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + cytarabine
ADP + cytarabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + cytarabine
ADP + cytarabine 5'-phosphate
show the reaction diagram
-
drug against acute myeloid and lymphoblastic leukemia, i.e. 1-beta-D-arabinofuranosylcytosine
-
?
ATP + cytidine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + cytidine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + cytidine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + cytidine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + cytidine
ADP + ?
show the reaction diagram
-
poor: beta-isomer, not: alpha-isomer
-
?
ATP + cytidine
ADP + cytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + D-2'-deoxycytidine
ADP + D-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + D-deoxycytidine
ADP + D-dCMP
show the reaction diagram
-
-
-
?
ATP + D-deoxythymidine
ADP + D-dTMP
show the reaction diagram
-
-
-
?
ATP + D-deoxyuridine
ADP + D-dUMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
Bacillus megaterium KM
-
-
-
?
ATP + deoxycytidine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + ?
show the reaction diagram
-
key enzyme in anabolic phosphorylation of deoxyribonucleosides and their analogues
-
?
ATP + deoxycytidine
ADP + ?
show the reaction diagram
-
key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
?
ATP + dideoxythymidine
ADP + dideoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + difluoro-arabinofuranosyl adenine
ADP + difluoro-arabinofuranosyl adenine 5'-phosphate
show the reaction diagram
i.e. fludarabine
-
?
ATP + difluorodeoxycytidine
ADP + difluorodeoxycytidine 5'-phosphate
show the reaction diagram
i.e. gemcitabine
-
?
ATP + fludarabine
ADP + fludarabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + fludarabine
ADP + fludarabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + fludarabine
ADP + phospho-fludarabine
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine 5'-phosphate
show the reaction diagram
-
i.e. difluorodeoxycytidine
-
?
ATP + gemcitabine
ADP + phospho-gemcitabine
show the reaction diagram
-
-
-
?
ATP + L-2',3'-dideoxy-3'-thiacytidine
ADP + L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + L-2'-deoxycytidine
ADP + L-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + L-deoxythymidine
ADP + L-dTMP
show the reaction diagram
-
-
-
?
ATP + L-deoxyuridine
ADP + L-dUMP
show the reaction diagram
-
-
-
?
ATP + L-thymidine
?
show the reaction diagram
-
human dCK, in addition to being able to phosphorylate both purines and pyrimidines, has the special ability to accept L-nucleosides as substrates
-
-
?
ATP + lamivudine
ADP + lamivudine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + TMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + TMP
show the reaction diagram
-
cytosolic enzyme, poor substrate
-
?
ATP + troxacitabine
ADP + troxacitabine 5'-phosphate
show the reaction diagram
-
-
-
?
beta-D-3'-hydroxymethyl-2',3'dideoxycytidine + ATP
ADP + beta-D-3'-hydroxymethyl-2',3'dideoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
CTP + 2'-deoxycytidine
CDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
-
CTP + 2'-deoxycytidine
CDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
CTP + 2'-deoxycytidine
CDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
CTP + 2'-deoxycytidine
CDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
CTP + 2'-deoxycytidine
CDP + 2'-deoxy-CMP
show the reaction diagram
-
poor
-
?
CTP + 2'-deoxycytidine
CDP + 2'-deoxy-CMP
show the reaction diagram
-
as good as GTP, UTP, dATP, mitochondrial isozyme
-
?
CTP + deoxycytidine
CDP + dCMP
show the reaction diagram
Bacillus megaterium, Bacillus megaterium KM
-
-
-
?
dATP + deoxycytidine
dADP + dCMP
show the reaction diagram
Bacillus megaterium, Bacillus megaterium KM
-
-
-
?
dCTP + 2'-deoxycytidine
dCDP + 2'-deoxy-CMP
show the reaction diagram
-
only in the absence of ATP
-
?
dCTP + deoxycytidine
dCDP + dCMP
show the reaction diagram
Bacillus megaterium, Bacillus megaterium KM
-
-
-
?
dGTP + 2'-deoxycytidine
dGDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dGTP + 2'-deoxycytidine
dGDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dGTP + 2'-deoxycytidine
dGDP + 2'-deoxy-CMP
show the reaction diagram
-
cytosolic isozyme I
-
?
dGTP + 2'-deoxycytidine
dGDP + 2'-deoxy-CMP
show the reaction diagram
-
best phosphate donor
-
?
dGTP + 2'-deoxycytidine
dGDP + 2'-deoxy-CMP
show the reaction diagram
-
best phosphate donor
-
?
dGTP + 2'-deoxycytidine
dGDP + 2'-deoxy-CMP
show the reaction diagram
-
poor substrate of mitochondrial isozyme
-
?
dGTP + deoxycytidine
dGDP + dCMP
show the reaction diagram
Bacillus megaterium, Bacillus megaterium KM
-
-
-
?
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
-
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dTTP + 2'-deoxycytidine
dTDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
dTTP + deoxycytidine
dTDP + dCMP
show the reaction diagram
-
-
-
?
dUTP + 2'-deoxycytidine
dUDP + 2'-deoxy-CMP
show the reaction diagram
-
best phosphate donor
-
?
dUTP + 2'-deoxycytidine
dUDP + 2'-deoxy-CMP
show the reaction diagram
-
best phosphate donor, cytosolic enzyme I, poor, mitochondrial isozyme
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
-
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
as good as ATP
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
best phosphate donor
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxy-CMP
show the reaction diagram
-
as good as dGTP, dTTP or dUTP, cytosolic isozyme I, as good as CTP, dATP or UTP, mitochondrial isozyme
-
?
NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
activation of the clinically relevant substrate
-
?
NTP + 1-beta-D-arabinofuranosylcytosine
NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
antineoplastic agent
-
?
NTP + 1-beta-D-arabinofuranosylcytosine
NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
i.e. cytarabine, rate limiting enzyme in the activation of the prodrug
-
?
NTP + 1-beta-D-arabinosylcytosine
NDP + 1-beta-D-arabinosylcytosine 5'-phosphate
show the reaction diagram
-
i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 2',2'-difluoro-2'-deoxycytidine
NDP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
best acceptor substrate, best phosphate donors: UTP or NTP-mixture, better than ATP
-
?
NTP + 2',2'-difluoro-2'-deoxycytidine
NDP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
cytosolic isozyme I, not mitochondrial isozyme
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, rate limiting enzyme in the activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, rate limiting enzyme in the activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, rate limiting enzyme in the activation of the pharmaceutically relevant substrate which is commonly used in the treatment of tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
substrate also termed gemcitabine
-
?
NTP + 2',3'-dideoxycytidine
NDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2',3'-dideoxycytidine
NDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
poor
-
?
NTP + 2',3'-dideoxycytidine
NDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
antiviral agent
-
?
NTP + 2',3'-dideoxycytidine
NDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections
-
?
NTP + 2'-deoxy-3'-thiacytidine
NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
antiviral agent
-
?
NTP + 2'-deoxy-3'-thiacytidine
NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections
-
?
NTP + 2'-deoxyadenosine
NDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2'-deoxyadenosine
NDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
NTP + 2'-deoxycytidine
NDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2'-deoxycytidine
NDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine
-
?
NTP + 2'-deoxycytidine
NDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
NTP + 2'-deoxyguanosine
NDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2'-deoxyguanosine
NDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine, antineoplastic agent
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
phosphorylation of chemotherapeutically important nucleoside analogues
-
?
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine
NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
show the reaction diagram
-
i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies
-
?
NTP + 9-beta-D-arabinofuranosyl-2-fluoroadenine
NDP + 9-beta-D-arabinofuranosyl-2-fluoroadenine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 9-beta-D-arabinosyl 2-fluoroadenine
NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
show the reaction diagram
-
activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 9-beta-D-arabinosyl 2-fluoroadenine
NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
show the reaction diagram
-
i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 9-beta-D-arabinosyl-2-fluoroadenine
NDP + 9-beta-D-arabinosyl-2-fluoroadenine 5'-phosphate
show the reaction diagram
-
phosphorylation of chemotherapeutically important nucleoside analogues
-
?
NTP + beta-D-2'-deoxythioguanosine
NDP + ?
show the reaction diagram
-
not alpha-isomer
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
-
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
ATP can be replaced by GTP, dATP, dGTP or dTTP
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
D-isomer
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
D-isomer, best phosphate donors: UTP or NTP-mixture, even better than ATP
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
cytosolic isozyme I, no acceptor substrate for cytosolic isozyme II or mitochondrial isozyme
-
?
NTP + cytosine arabinoside
NDP + cytosine arabinoside 5'-monophosphate
show the reaction diagram
-
i.e. 1-beta-arabinofuranosyl cytosine, L-isomer better substrate than D-isomer
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
-
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
poor
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
-
cytosolic isozyme I, not mitochondrial isozyme
-
?
NTP + deoxyadenosine
NDP + dAMP
show the reaction diagram
Lactobacillus acidophilus R-26
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
specificity
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: phosphate
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: dCTP
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: dCTP
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: guanosine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: guanosine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
poor substrate: arabinosyl-CTP
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
enzyme shows little specificity towards phosphate donor
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
enzyme shows little specificity towards phosphate donor
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
enzyme shows little specificity towards phosphate donor
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
enzyme shows little specificity towards phosphate donor
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
enzyme shows little specificity towards phosphate donor
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
in order of decreasing activity: dGTP, CTP, ATP, dATP, dTTP, UTP, GTP
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: beta-gamma-methylene diphosphonate analogue of dTTP
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: ribavirin
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: uridine arabinoside, cytosine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
beta-D-2'-deoxyribonucleosides are more efficient than corresponding beta-D-arabinonucleosides or beta-ribonucleosides, rather nonspecific for base moiety of nucleoside substrate
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: adenosine, uridine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: adenosine, uridine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: adenosine, uridine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: nucleoside diphosphates and their deoxy derivatives
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
poor substrate: beta-5-azacytidine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: deoxyuridine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
best acceptor substrate at low substrate concentrations
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
no substrate: thymidine
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
Lactobacillus acidophilus R-26
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
-
-
?
NTP + deoxyguanosine
NDP + dGMP
show the reaction diagram
-
cytosolic isozyme I, not mitochondrial isozyme
-
?
NTP + deoxythymidine
NDP + dTMP
show the reaction diagram
-
mitochondrial isozyme, better than deoxycytidine, not cytosolic isozyme I
-
?
NTP + deoxyuridine
NDP + dUMP
show the reaction diagram
-
mitochondrial isozyme, not cytosolic isozyme I
-
?
NTP + deoxyuridine
NDP + dUMP
show the reaction diagram
-
cytosolic enzyme, poor substrate
-
?
NTP + troxacitabine
?
show the reaction diagram
-
activation of the clinically relevant substrate
-
-
?
UTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
UDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 1-beta-D-arabinofuranosylcytosine
UDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
i.e. cytarabine
-
?
UTP + 2',2'-difluorodeoxycytidine
UDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine
-
?
UTP + 2',3'-dideoxycytidine
UDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2',3'-dideoxycytidine
UDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
i.e. zalcitabine
-
?
UTP + 2',3'-dideoxycytidine
UDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used for the treatment of HIV infections
-
?
UTP + 2'-deoxy-3'-thiacytidine
UDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxy-3'-thiacytidine
UDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used for the treatment of HIV infections
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
poor
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
best phosphate donor at low substrate levels, as good as CTP, GTP or dATP
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
as good as CTP, GTP or dATP
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxy-CMP
show the reaction diagram
-
as good as CTP, GTP or dATP
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
preferred donor and acceptor substrates
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
UTP + 2-chloro-2'-deoxyadenosine
UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
i.e. cladribine
-
?
UTP + 2-chloro-2'-deoxyadenosine
UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
UTP + 2-chlorodeoxyadenosine
UDP + 2-chlorodeoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
UTP + 2-fluoro-9-beta-D-arabinofuranosyladenine
UDP + 2-fluoro-9-beta-D-arabinofuranosyladenine 5'-phosphate
show the reaction diagram
-
i.e. fludarabine
-
?
UTP + 9-beta-D-arabinofuranosylguanine
UDP + 9-beta-D-arabinofuranosylguanine 5'-phosphate
show the reaction diagram
-
i.e. nelarabine
-
?
UTP + adenosine
UDP + adenosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + arabinosyl adenine
UDP + arabinosyl adenine 5'-phosphate
show the reaction diagram
-
-
?
UTP + arabinosyl cytosine
UDP + arabinosyl cytosine 5'-phosphate
show the reaction diagram
-
i.e. cytosar
-
?
UTP + arabinosyl cytosine
UDP + arabinosyl cytosine 5'-phosphate
show the reaction diagram
i.e. cytosar
-
?
UTP + beta-L-2',3'-dideoxy-3'-thiacytidine
UDP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
?
UTP + beta-L-dioxolane-cytidine
UDP + beta-L-dioxolane-cytidine 5'-phosphate
show the reaction diagram
antitumor drug
-
?
UTP + cladribine
UDP + cladribine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + cladribine
UDP + phospho-cladribine
show the reaction diagram
-
-
-
?
UTP + clofarabine
UDP + phospho-clofarabine
show the reaction diagram
-
-
-
?
UTP + cytidine
UDP + cytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + D-2'-deoxycytidine
UDP + D-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
UTP is physiological phosphate donor
-
?
UTP + deoxyguanosine
UDP + dGMP
show the reaction diagram
-
-
-
?
UTP + deoxyguanosine
UDP + dGMP
show the reaction diagram
-
-
-
?
UTP + difluoro-arabinofuranosyl adenine
UDP + difluoro-arabinofuranosyl adenine 5'-phosphate
show the reaction diagram
i.e. fludarabine
-
?
UTP + difluorodeoxycytidine
UDP + difluorodeoxycytidine 5'-phosphate
show the reaction diagram
i.e. gemcitabine
-
?
UTP + fludarabine
UDP + fludarabine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + fludarabine
UDP + phospho-fludarabine
show the reaction diagram
-
-
-
?
UTP + gemcitabine
UDP + gemcitabine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + L-2',3'-dideoxy-3'-thiacytidine
UDP + L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + L-2'-deoxycytidine
UDP + L-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + troxacitabine
UDP + troxacitabine 5'-phosphate
show the reaction diagram
-
-
-
?
GTP + deoxycytidine
GDP + dCMP
show the reaction diagram
-
optimal activity with GTP as phosphoryl donor
-
?
additional information
?
-
-
purine deoxynucleoside activity inseparably associated with deoxycytidine kinase protein
-
-
-
additional information
?
-
-
multisubstrate enzyme, that also phosphorylates purine deoxyribonucleotides
-
-
-
additional information
?
-
-
remarkably relaxed enantioselectivity with respect to cytidine derivatives in beta configuration
-
-
-
additional information
?
-
-
enzyme has two separate active sites for deoxycytidine and deoxyadenosine activity
-
-
-
additional information
?
-
-
several isozymes: cytosolic deoxycytidine kinase I and II, plus mitochondrial isozyme
-
-
-
additional information
?
-
-
lack of enantioselectivity for D- and L-analogues of cytidine and adenosine
-
-
-
additional information
?
-
-
presumably one common nucleoside acceptor site
-
-
-
additional information
?
-
-
enzyme exists in different conformational states with different substrate kinetic properties
-
-
-
additional information
?
-
-
reacts with both enantiomers of beta-deoxycytidine, beta-deoxyguanidine, beta-deoxyadenosine, and alpha-D-deoxycytidine is also substrate
-
-
-
additional information
?
-
-
reacts with both enantiomers of beta-deoxyadenosine, beta-arabinofuranosyl-adenine and beta-deoxyguanine
-
-
-
additional information
?
-
-
5'-phosphorylation of is a crucial step in metabolic activation of anticancer and antiviral nucleoside antimetabolites, such as cladribine, gemcitabine, cytarabine, and lamivudine
-
-
-
additional information
?
-
-
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
-
-
-
additional information
?
-
-
enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
-
-
-
additional information
?
-
-
resistance of cells to cytarabine is caused by dCK enzyme deficiency
-
-
-
additional information
?
-
-
the activation of the enzyme is a first step in 2-chloro-2'-deoxyadenosine-induced cytotoxicity
-
-
-
additional information
?
-
-
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
-
-
-
additional information
?
-
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
-
-
-
additional information
?
-
-
the enzyme is active and important in activation of several clinically important deoxynucleoside analogues used for the treatment of haematological and solid malignancies
-
-
-
additional information
?
-
-
broad substrate specificity, overview, binding of acceptor subtrates is more tightly in presence of phosphate donor molecules
-
-
-
additional information
?
-
-
dioxolane guanosine is no substrate
-
-
-
additional information
?
-
-
enzyme can utilizes several phosphate donors, with preference for UTP
-
-
-
additional information
?
-
-
no activity with several difluorophenyl nucleoside analogues and with 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril, overview
-
-
-
additional information
?
-
substrate-enzyme interactions, overview, enzyme prefers nucleosides with sugars in the S-conformation, i.e C2'-endo-C3'-exo, binding of substrate induces conformational changes, the phosphate donor influences the acceptor substrate specificity, mechanism, overview
-
-
-
additional information
?
-
-
the enzyme shows broad substrate specificity
-
-
-
additional information
?
-
-
catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy
-
-
-
additional information
?
-
-
dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs
-
-
-
additional information
?
-
-
enzyme of the salvage pathway for deoxyribonucleotide synthesis, which provides resting cells with deoxynucleotides for DNA repair and mitochondrial DNA synthesis, important enzyme for the phosphorylation/activation of deoxynucleoside analogues, which are cytotoxic towards both replicating and indolent malignancies
-
-
-
additional information
?
-
-
essential for the synthesis of deoxynucleotides required for DNA repair and involved in deoxynucleoside analogue activation
-
-
-
additional information
?
-
-
key enzyme in the deoxynucleoside salvage pathway and in the activation of numerous nucleoside analogues used in cancer and antiviral chemotherapy
-
-
-
additional information
?
-
-
phosphorylates both pyrimidine and purine deoxynucleosides, including numerous nucleoside analogue anticancer and antiviral prodrugs
-
-
-
additional information
?
-
-
dCK phosphorylates all three natural deoxyribonucleosides, exhibiting the highest affinity for 2'-deoxycytidine
-
-
-
additional information
?
-
-
dCK interacts with cyclin-dependent kinase 1 (Cdk1) and the interaction inhibits Cdk1 activity both in vitro and in vivo
-
-
-
additional information
?
-
-
development and evaluation of a homogeneous fluorescence-based assay for real-time and simultaneous detection of thymidine kinase 1 and deoxycytidine kinase activities, detailed overview
-
-
-
additional information
?
-
-
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
-
-
-
additional information
?
-
-
deoxycytidine kinase shows broad substrate specificity, catalyzing the phosphorylation of deoxycytidine, deoxyadenosine, and deoxyguanosine with ATP or UTP as phosphoryl donor
-
-
-
additional information
?
-
-
substrate specificity or wild-type an dmutant enzymes, overview
-
-
-
additional information
?
-
-
the human enzyme is not active with the acyclic guanine analogue acyclovir. Acyclovir binds at the dCK active site, but does so adopting a nonproductive conformation. Despite binding ACV, the enzyme remains in the open, inactive state, overview. dCK would phosphorylate acyclic guanine analogs if they can induce a similar rotation
-
-
-
additional information
?
-
Mus musculus C57BL/6J
-
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine monophosphate
show the reaction diagram
-
i.e. gemcitabine, transport and metabolism in vivo, overview
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
P43346
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
P27707
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
P43346
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
involved in nucleotide synthesis, involved in DNA repair
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
P43346
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine
-
?
ATP + 5-methyldeoxycytidine
ADP + 5-methyl-dCMP
show the reaction diagram
-
-
-
?
ATP + cladribine
ADP + phospho-cladribine
show the reaction diagram
-
-
-
?
ATP + clofarabine
ADP + phospho-clofarabine
show the reaction diagram
-
-
-
?
ATP + clofarabine
ADP + phospho-clofarabine
show the reaction diagram
-
-
-
?
ATP + cytarabine
ADP + cytarabine 5'-phosphate
show the reaction diagram
-
drug against acute myeloid and lymphoblastic leukemia
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + ?
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + ?
show the reaction diagram
-
key enzyme in anabolic phosphorylation of deoxyribonucleosides and their analogues
-
?
ATP + deoxycytidine
ADP + ?
show the reaction diagram
-
key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
?
ATP + fludarabine
ADP + phospho-fludarabine
show the reaction diagram
-
-
-
?
NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
activation of the clinically relevant substrate
-
?
NTP + 1-beta-D-arabinofuranosylcytosine
NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
antineoplastic agent
-
?
NTP + 1-beta-D-arabinofuranosylcytosine
NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
show the reaction diagram
-
i.e. cytarabine, rate limiting enzyme in the activation of the prodrug
-
?
NTP + 1-beta-D-arabinosylcytosine
NDP + 1-beta-D-arabinosylcytosine 5'-phosphate
show the reaction diagram
-
i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, rate limiting enzyme in the activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, rate limiting enzyme in the activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. gemcitabine, rate limiting enzyme in the activation of the pharmaceutically relevant substrate which is commonly used in the treatment of tumors
-
?
NTP + 2',2'-difluorodeoxycytidine
NDP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
-
substrate also termed gemcitabine
-
?
NTP + 2',3'-dideoxycytidine
NDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
antiviral agent
-
?
NTP + 2',3'-dideoxycytidine
NDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections
-
?
NTP + 2'-deoxy-3'-thiacytidine
NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
antiviral agent
-
?
NTP + 2'-deoxy-3'-thiacytidine
NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections
-
?
NTP + 2'-deoxyadenosine
NDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2'-deoxyadenosine
NDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
NTP + 2'-deoxycytidine
NDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
more effective substrate than 2'-deoxyadenosine and 2'-deoxyguanosine
-
?
NTP + 2'-deoxycytidine
NDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
NTP + 2'-deoxyguanosine
NDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
NTP + 2'-deoxyguanosine
NDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
i.e. cladribine, antineoplastic agent
-
?
NTP + 2-chloro-2'-deoxyadenosine
NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
phosphorylation of chemotherapeutically important nucleoside analogues
-
?
NTP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine
NDP + 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine 5'-phosphate
show the reaction diagram
-
i.e. clofarabine, rate-limiting step in the activation of the prodrug, precursor of an effective chemotherapeutic agent for leukemias and other haematological malignancies
-
?
NTP + 9-beta-D-arabinosyl 2-fluoroadenine
NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
show the reaction diagram
-
activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 9-beta-D-arabinosyl 2-fluoroadenine
NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate
show the reaction diagram
-
i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies
-
?
NTP + 9-beta-D-arabinosyl-2-fluoroadenine
NDP + 9-beta-D-arabinosyl-2-fluoroadenine 5'-phosphate
show the reaction diagram
-
phosphorylation of chemotherapeutically important nucleoside analogues
-
?
NTP + deoxycytidine
NDP + dCMP
show the reaction diagram
-
-
-
?
NTP + troxacitabine
?
show the reaction diagram
-
activation of the clinically relevant substrate
-
-
?
UTP + 2',3'-dideoxycytidine
UDP + 2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used for the treatment of HIV infections
-
?
UTP + 2'-deoxy-3'-thiacytidine
UDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
show the reaction diagram
-
i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used for the treatment of HIV infections
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
P27707
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
preferred donor and acceptor substrates
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
UTP + 2'-deoxycytidine
UDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides, supplying cells with deoxyribonucleotides for DNA replicative and repair synthesis
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + 2'-deoxyguanosine
UDP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
rate-limiting reaction in the salvage of deoxyribonucleosides
-
?
UTP + cladribine
UDP + phospho-cladribine
show the reaction diagram
-
-
-
?
UTP + clofarabine
UDP + phospho-clofarabine
show the reaction diagram
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
-
-
?
UTP + deoxycytidine
UDP + dCMP
show the reaction diagram
-
UTP is physiological phosphate donor
-
?
UTP + deoxyguanosine
UDP + dGMP
show the reaction diagram
-
-
-
?
UTP + deoxyguanosine
UDP + dGMP
show the reaction diagram
-
-
-
?
UTP + fludarabine
UDP + phospho-fludarabine
show the reaction diagram
-
-
-
?
ATP + L-thymidine
?
show the reaction diagram
-
human dCK, in addition to being able to phosphorylate both purines and pyrimidines, has the special ability to accept L-nucleosides as substrates
-
-
?
additional information
?
-
-
5'-phosphorylation of is a crucial step in metabolic activation of anticancer and antiviral nucleoside antimetabolites, such as cladribine, gemcitabine, cytarabine, and lamivudine
-
-
-
additional information
?
-
-
de novo synthesis and salvage of ribonucleotides and deoxyribonucleotides, metabolism overview, enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
-
-
-
additional information
?
-
-
enzyme is active and medically important in anticancer and antiviral therapy due to high phosphorylation activity of prodrugs, overview
-
-
-
additional information
?
-
-
resistance of cells to cytarabine is caused by dCK enzyme deficiency
-
-
-
additional information
?
-
-
the activation of the enzyme is a first step in 2-chloro-2'-deoxyadenosine-induced cytotoxicity
-
-
-
additional information
?
-
-
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
-
-
-
additional information
?
-
P43346
the earliest nucleotide kinase in evolution, probably encoded by the so-called dCK/dGK/TK2-like gene, is the progenitor gene, several duplications of it are the reason for evolutionary occurrence of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, overview
-
-
-
additional information
?
-
-
the enzyme is active and important in activation of several clinically important deoxynucleoside analogues used for the treatment of haematological and solid malignancies
-
-
-
additional information
?
-
-
catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy
-
-
-
additional information
?
-
-
dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs
-
-
-
additional information
?
-
-
enzyme of the salvage pathway for deoxyribonucleotide synthesis, which provides resting cells with deoxynucleotides for DNA repair and mitochondrial DNA synthesis, important enzyme for the phosphorylation/activation of deoxynucleoside analogues, which are cytotoxic towards both replicating and indolent malignancies
-
-
-
additional information
?
-
-
essential for the synthesis of deoxynucleotides required for DNA repair and involved in deoxynucleoside analogue activation
-
-
-
additional information
?
-
-
key enzyme in the deoxynucleoside salvage pathway and in the activation of numerous nucleoside analogues used in cancer and antiviral chemotherapy
-
-
-
additional information
?
-
-
phosphorylates both pyrimidine and purine deoxynucleosides, including numerous nucleoside analogue anticancer and antiviral prodrugs
-
-
-
additional information
?
-
-
dCK phosphorylates all three natural deoxyribonucleosides, exhibiting the highest affinity for 2'-deoxycytidine
-
-
-
additional information
?
-
-
dCK interacts with cyclin-dependent kinase 1 (Cdk1) and the interaction inhibits Cdk1 activity both in vitro and in vivo
-
-
-
additional information
?
-
-
development and evaluation of a homogeneous fluorescence-based assay for real-time and simultaneous detection of thymidine kinase 1 and deoxycytidine kinase activities, detailed overview
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
UTP
-
preferred phosphate donor
UTP
-
preferred, in vivo important phoaphate donor
UTP
-
preferred phosphoryl group donor
additional information
-
enzyme lalso utilizes other nucleoside triphosphates as phosphate donors
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
activation; can replace Mn2+ or Mg2+ to some extent
Ca2+
-
activation; can replace Mn2+ or Mg2+ to some extent
Ca2+
-
about 55% as efficient as Mg2+; activation
Ca2+
-
activation
Ca2+
-
activation
CdCl2
-
activation, in the absence of dithiothreitol
Co2+
-
about 40% as efficient as Mg2+; activation
Co2+
-
activation
Co2+
-
about 30% as efficient as Mg2+; activation
Fe2+
-
activation; can replace Mn2+ or Mg2+ to some extent
Fe2+
-
about 45% as efficient as Mg2+; activation
Fe2+
-
activation
Fe2+
-
about 60% as efficient as Mg2+; activation
Mg2+
-
requirement, 2 mM
Mg2+
-
more efficient than Mn2+ or Ca2+
Mg2+
-
Mg2+/ATP complex is the reactive species
Mg2+
-
below: decrease of activity; maximal activity at a ATP:Mg2+-ratio of 1:1; Mg2+/ATP complex is the reactive species
Mg2+
-
maximal activity at a ATP:Mg2+-ratio of 1:1; Mg2+/ATP complex is the reactive species
Mg2+
-
Mg2+/ATP complex is the reactive species
Mg2+
-
in complex with ATP
Mg2+
-
in complex with ATP or UTP
Mg2+
-
required
Mg2+
-
required
Mn2+
-
0.5 mM; requirement
Mn2+
-
about 80% as efficient as Mg2+; requirement
Mn2+
-
requirement
Ni2+
-
about 20% as efficient as Mg2+; activation
Ni2+
-
activation
Ni2+
-
not
Sr2+
-
about 15% as efficient as Mg2+; activation
Sr2+
-
activation
Zn2+
-
not
Zn2+
-
about 40% as efficient as Mg2+; activation
Zn2+
-
activation
Zn2+
-
about 30% as efficient as Mg2+; activation
Mn2+
-
about 90% as efficient as Mg2+; requirement
additional information
-
no activation by Ba2+, Cu2+
additional information
-
no activation by Ba2+, Cu2+
additional information
-
no activation by Ba2+, Cu2+; no activation by monovalent cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2'-deoxy-5-fluorocytidine
-
-
2'-deoxycytidine
-
-
2'-O-methylcytidine
-
-
2-(2-[1-[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]piperidin-4-yl]ethoxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]piperidin-4-yl]methoxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(2-chlorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(2-ethylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(3-chlorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(4-chloro-2-methylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(4-chlorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([1-[6-(biphenyl-2-yloxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
-
-
2-([6-[4-(4-amino-2-oxopyrimidin-1(2H)-yl)piperidin-1-yl]-5-fluoropyrimidin-4-yl]oxy)benzonitrile
-
-
2-chloroadenosine
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-2-bromopyrimidin-4-yl)oxy]benzonitrile
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-2-methylpyrimidin-4-yl)oxy]benzonitrile
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]-5-methoxybenzonitrile
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]benzonitrile
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-methylpyrimidin-4-yl)oxy]benzonitrile
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]pyrimidin-4-yl)oxy]-5-methoxybenzonitrile
-
-
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]pyrimidin-4-yl)oxy]benzonitrile
-
-
2-[(6-[4-[(4-aminopyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]benzonitrile
-
-
2-[[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]oxy]-5-fluoropyrimidin-4-amine
-
-
3'-O-methyl-2'deoxycytidine
-
-
3'-[[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]sulfamoyl]biphenyl-4-carboxamide
-
-
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-2-fluoropyrimidin-4-yl)oxy]-4-methoxybenzonitrile
-
-
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]-4-methoxybenzonitrile
-
-
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]benzonitrile
-
-
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]pyrimidin-4-yl)oxy]benzonitrile
-
-
4'-thio-beta-D-arabinofuranosylcytosine
-
IC50 0.000024 mM
4-(1-benzothiophen-2-yl)-6-[4-(2-oxo-2-pyrrolidin-1-ylethyl)piperazin-1-yl]pyrimidine
-
-
4-(1-benzothiophen-2-yl)-6-[4-(2-oxo-2-pyrrolidin-1-ylethyl)piperazin-1-yl]pyrimidine
-
-
5'-O-ethyl-2'-deoxycytidine
-
-
5'-O-methyl-2'-deoxycytidine
-
-
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine
-
IC50 0.051 mM
5-Fluoro-2'-deoxycytidine
-
-
5-Fluoro-2'-deoxycytidine
-
competitive inhibitor
5-fluoro-2-([1-[5-fluoro-6-(4-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-([1-[6-(2-fluorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-([1-[6-(2-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-([1-[6-(2-methylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-([1-[6-(3-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-([1-[6-(4-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-([1-[6-(4-methylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
-
-
5-fluoro-2-[[1-(6-phenoxypyrimidin-4-yl)piperidin-4-yl]oxy]pyrimidin-4-amine
-
-
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
-
IC50 0.0000020 mM
adenosine arabinoside
-
weak, not with deoxyadenosine as substrate
ADP
-
not
ADP
-
-
ADP
-
kinetics
alpha-D-2'-Deoxythioguanosine
-
-
Ba2+
-
-
CDP
-
strong
CMP
-
not
CTP
-
strong, ATP as substrate
CTP
-
-
Cu2+
-
-
cytidine
-
weak
cytidine
-
not
cytidine
-
cytosine arabinoside as substrate
cytidine
-
not
cytidine
-
alpha-isomer
cytidine
-
deoxycytidine as substrate
cytosine arabinoside
-
ATP as substrate; deoxycytidine as substrate; strong, deoxyadenosine as substrate
cytosine arabinoside
-
deoxycytidine as substrate
cytosine arabinoside
-
deoxycytidine as substrate
cytosine arabinoside
-
deoxyguanosine as substrate
cytosine arabinoside
-
deoxycytidine as substrate
cytosine arabinoside
-
IC50 0.0000055 mM
Cytosine arabinoside monophosphate
-
strong
Cytosine arabinoside triphosphate
-
strong
Cytosine arabinoside triphosphate
-
-
Cytosine arabinoside triphosphate
-
weak
dADP
-
strong
dAMP
-
not
dAMP
-
not
dAMP
-
strong
dATP
-
strong
dATP
-
strong
dCDP
-
-
dCDP
-
strong, ATP as substrate
dCDP
-
-
dCMP
-
-
dCMP
-
ATP as substrate; product inhibition, strong
dCMP
-
dTTP reverses; product inhibition, strong
dCMP
-
only cytosolic isozyme I; product inhibition, strong
dCMP
-
product inhibition, strong
dCMP
-
-
dCTP
-
strong, pH-dependent
dCTP
-
-
dCTP
-
reversible by dTTP; reversible by UTP, dUTP
dCTP
-
kinetics; reversible by dTTP; reversible by UDP
dCTP
-
ATP as substrate
dCTP
-
no reversion by beta-gamma-methylene diphosphonate analogue of dTTP
dCTP
-
cytosine arabinoside as substrate; isozyme II
dCTP
-
kinetics
dCTP
-
no inhibition at physiological concentrations
dCTP
-
cytosine arabinoside as substrate
dCTP
-
ATP as substrate; moderate phosphate donor in the absence of ATP
dCTP
-
at 0.013 mM, substrates ATP, deoxycytosine, 30% inhibition
dCTP
-
at 0.013 mM, substrates ATP, deoxycytosine, 50% inhibition
dCTP
-
feedback inhibition, competitive versus phosphate donors
dCTP
-
feedback inhibition
dCTP
-
a potent inhibitor of the enzyme when ATP is the phosphoryl donor, but a relatively weak inhibitor when the enzyme activity is measured with UTP as the phosphoryl donor. Inhibition is higher with respect to cladribine than deoxycytidine
deoxyadenosine
-
-
deoxyadenosine
-
not
deoxyadenosine
-
cytosine arabinoside or deoxyguanosine as substrate
deoxyadenosine
-
cytosolic isozyme I, weak
deoxyadenosine
-
deoxycytidine
deoxyadenosine
-
cytosolic isozyme I, weak; deoxycytidine; kinetics
deoxycytidine
-
deoxyadenosine as substrate
deoxycytidine
-
cytosine arabinoside as substrate
deoxycytidine
-
cytosine arabinoside as substrate; deoxyadenosine as substrate; deoxyguanosine as substrate
deoxycytidine
-
cytidine as substrate; deoxyguanosine as substrate
deoxycytidine
-
deoxyadenosine as substrate; deoxyguanosine as substrate
deoxycytidine
-
cytosine arabinoside as substrate
deoxycytidine
-
at concentrations greater than 0.003 mM, UTP as substrate, noncompetitive
deoxyguanosine
-
cytosine arabinoside as substrate
deoxyguanosine
-
not
deoxyguanosine
-
deoxyguanosine as substrate, cytosolic isozyme I, weak
deoxyguanosine
-
deoxycytidine as substrate
deoxyguanosine
-
-
deoxythymidine
-
cytosolic isozyme I, not
deoxythymidine
-
weak
dGDP
-
strong
dGDP
-
not
dGMP
-
not
dGMP
-
strong
dGTP
-
ATP as substrate
dGTP
-
-
dGTP
-
ATP as substrate
Dideoxyadenosine
-
-
Dideoxycytidine
-
-
Dinitrophenol
-
weak
Dioxane
-
-
dTDP
-
strong
dTTP
-
ATP as substrate; weak
dTTP
-
at increased deoxycytidine concentrations
dTTP
-
-
dTTP
-
only mitochondrial isozyme; strong
dTTP
-
ATP as substrate; weak
dUDP
-
strong
dUDP
-
only cytosolic isozyme I; strong
dUMP
-
not
dUMP
-
not
dUMP
-
strong
dUTP
-
-
GDP
-
not
GDP
-
-
GSSG
-
thiol reagents protect or reverse
GTP
-
not, deoxyadenosine as substrate; weak, ATP and deoxycytidine as substrate
GTP
-
-
GTP
-
not, deoxyadenosine as substrate
H-7
-
reduces the enzyme activation caused by the addition of 2-chloro-2-deoxyadenosine
HgCl2
-
-
HgCl2
-
-
HgCl2
-
strong, thiol reagents protect
HgCl2
-
-
IAA
-
weak
lambda protein phosphatase
-
-
-
LP-375752
-
i.e. 2-[4-(6-benzo[b]thiophen-2-yl-pyrimidin-4-yl)-piperazin-1-yl]-1-pyrrolidin-1-yl-ethanone
N-ethylmaleimide
-
weak
N-ethylmaleimide
-
thiol reagents protect or reverse
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-2',4'-dichlorobiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-2'-chlorobiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3'-chlorobiphenyl-3-carboxamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3'-chlorobiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3-bromobenzenesulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4''-chloro-2''-methyl-1,1':4',1''-terphenyl-3-carboxamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4''-chloro-2''-methyl-1,1':4',1''-terphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-(methylsulfonyl)biphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-chlorobiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-cyanobiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-formylbiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methoxybiphenyl-3-carboxamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methoxybiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methylbiphenyl-3-sulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4-(1-benzothiophen-2-yl)pyridine-2-carboxamide
-
potent, selective, and orally bioavailable inhibitor of dCK
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4-bromobenzenesulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4-nitrobenzenesulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]benzenesulfonamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]biphenyl-3-carboxamide
-
-
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]biphenyl-3-sulfonamide
-
-
N6-Phenyladenosine
-
-
NaCl
-
at 0.2-0.4 M, stimulation of 2-deoxycytidine and 2-chlorodeoxyadenosine phosphorylation, but inhibition of 2-deoxyguanosine phosphorylation
Non-substrate inhibitors
-
-
-
Nucleoside analogs
-
related to deoxycytidine, deoxyadenosine or deoxyguanosine
Nucleosides
-
overview, ATP and deoxyadenosine or deoxycytidine as substrate
Nucleosides
-
-
p-chloromercuribenzoate
-
thiol reagents protect or reverse
P1,P4-diadenosine 5'-tetraphosphate
-
not di-, tri- or pentaphosphate derivative
protein phosphatase lambda
-
-
-
Sodium diphosphate
-
not monophosphate
sorbitol
-
-
staurosporine
-
reduces the enzyme activation caused by the addition of 2-chloro-2-deoxyadenosine
Substrate derivatives
-
overview
-
suramin
-
no effect in unirradiated cells, complete abolishment of UV-C light induced activation
tetrahydrouridine
-
-
Tris-maleate buffer
-
-
Trypsin
-
substrates or dNTP protect corresponding active site
-
UDP
-
strong
UDP
-
only cytosolic isozyme I; strong
UDP
-
strong
UMP
-
not
UMP
-
strong
Urea
-
-
Uridine arabinoside
-
weak, cytosolic isozyme I
UTP
-
ATP as substrate; not with deoxyadenosine as substrate
UTP
-
-
UTP
-
not mitochondrial isozyme
LP-503392
-
i.e. 2-thio-2'-deoxycytidine
additional information
-
little or no inhibition by AMP, dTMP, adenosine, guanosine
-
additional information
-
little or no inhibition by deoxyuridine; no inhibition by TTP; no inhibition by uridine, thymidine; relatively insensitive to SH-inhibitors and detergents
-
additional information
-
little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine
-
additional information
-
no inhibition by ribavirin
-
additional information
-
deoxycytidine phosphorylating ability shows different inhibition pattern than deoxyadenosine activity
-
additional information
-
little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine; little or no inhibition by ribavirin; no inhibition by inosine, xanthosine, S-adenosylhomocysteine, S-adenosylmethionine; no inhibition by uridine, thymidine; overview
-
additional information
-
activity of enzyme is reduced by hyperosmotic treatment of cells
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-beta-D-arabinosylcytosine
-
i.e. cytarabine, 0.001 mM, stimulation
2'-deoxyadenosine/deoxycoformycin
-
no stimulation of enzyme activity by either compound if applied alone, with both compound at 0.01 mM 2.4fold activation occurs
-
2'-deoxythymidine-5'-thiosulphate
-
in intact cells, activation, reversed by 2-deoxycytidine
2-chloro-2'-arabino-fluoro-2'-deoxyadenosine
-
0.001 mM, stimulation
2-chloro-2'-deoxy-2'-fluoro-arabinosyladenine
-
stimulation
2-chloro-2'-deoxyadenosine
-
i.e. cladribine, 0.001 mM, stimulation
2-chloro-2'-deoxyadenosine
-
up to 0.01 mM, up to 4fold increase in activity
2-chloro-2'-deoxyadenosine
-
2.4fold activation at 0.003 mM, activation is slightly inhibited by 3-aminobenzamide, the activation of the enzyme is a first step in 2-chloro-2'-deoxyadenosine-induced cytotoxicity
2-chloro-2'-deoxyadenosine
-
-
2-chloro-2'-deoxyadenosine
-
increases activity by increasing phosphorylation of Ser74
2-fluoro-1-beta-D-arabinosyladenine
-
i.e. fludarabine, 0.001 mM, stimulation
ABCG2
-
the ABC transporter activates the enzyme and reduces intracellular accumulation of clofarabine
-
AG-490
-
-
aphidicolin
-
at 0.006-0.012 mM, 2-3fold activation
aphidicolin
-
-
aphidicolin
-
increases activity by increasing phosphorylation of Ser74
aphidicolin
-
increases dCK activity via Ser74 phosphorylation, effect of aphidicolin treatment on dCK activity in leukemic cells with substrate deoxycytidine and cladribine and other substrates, overview
arabinosylcytidine
-
stimulation
ATP
-
substrate activation, at high concentrations of deoxycytidine
bovine serum albumin
-
activation
-
calyculin A
-
inhibitor of protein phosphatases, activation
CHAPS
-
slight stimulation, deoxycytidine as substrate
daidzein
-
-
deoxyadenosine
-
activation, deoxycytidine as substrate, mitochondrial isozyme
deoxyguanosine
-
activation, deoxycytidine as substrate, mitochondrial isozyme
dithiothreitol
-
not
dithiothreitol
-
activation
dithiothreitol
-
activation
dithiothreitol
-
activation
etoposide
-
0.001 mM, stimulation
etoposide
-
-
etoposide
-
increases activity by increasing phosphorylation of Ser74
gamma-radiation
-
0.5-2 GY dosage, activation
-
genistein
-
-
NaCl
-
at 0.2-0.4 M, stimulation of 2-deoxycytidine and 2-chlorodeoxyadenosine phosphorylation, but inhibition of 2-deoxyguanosine phosphorylation
NaF
-
15 mM, 1 h, activity about twice as high as initial level
okadaic acid
-
-
paclitaxel
-
paclitaxel increases dCK (10% to 50%) activity
PD 153035
-
i.e. 4-(3-bromoanilino)-6,7-dimethoxy-quinazinoline
PD 98059
-
i.e. 2'-amino-3'-methoxyflavone, in absence of deoxycytidine
PD-98059
-
-
SB 203580
-
in absence of deoxycytidine
Sodium cholate
-
slight stimulation, deoxycytidine as substrate
TTP
-
stimulation, cytosolic isozyme I
tyrphostin A47
-
-
UDP
-
stimulation, mitochondrial isozyme
Uridine arabinoside
-
activation, deoxycytidine as substrate, mitochondrial isozyme
Zwittergent 3-14
-
slight stimulation, deoxycytidine as substrate
genistein
-
increases activity by increasing phosphorylation of Ser74
additional information
-
not activating: 2-chloro-riboadenosine
-
additional information
-
enzyme is activated by inhibitors of signal transduction enzymes, overview, deoxycytidine prevents activation of the enzyme, no activation by PD 98059 and SB 203580 in presence of deoxycytidine
-
additional information
-
2- to 3-fold activation of enzyme by UV-C light irradiation
-
additional information
-
enzyme protein level increases when cells are incubated with cyclopentenyl cytosine
-
additional information
-
UV-C irradiation increases activity by increasing phosphorylation of Ser74
-
additional information
-
irradiation of intact cells increases enzyme activity, upregulation of activity might be caused by phosphorylation
-
additional information
-
the enzyme is activated in response to DNA damage. The ataxia-telangiectasia-mutated (ATM) kinase phosphorylates dCK on Serine 74 to activate it in response to DNA damage
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.003
(-)-beta-2',3'-dideoxy-3'-thiacytidine
-
wild type enzyme with ATP as phosphate donor, 37C, pH 7.5
0.008
(-)-beta-2',3'-dideoxy-3'-thiacytidine
-
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37C, pH 7.5; wild type enzyme with UTP as phosphate donor, 37C, pH 7.5
0.015
(-)-beta-2',3'-dideoxy-3'-thiacytidine
-
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37C, pH 7.5
0.00076
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
pH 7.6, 37C, recombinant enzyme
0.00654
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
-
pH 7.6, 37C, recombinant enzyme
0.00061
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
-
pH 7.6, 37C, recombinant enzyme
0.00102
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
-
pH 7.6, 37C, recombinant enzyme
0.003
1-beta-D-arabinofuranosylcytosine
-
residues 65-79 deletion mutant with UTP as cosubstrate; wild type enzyme with UTP as cosubstrate
0.0059
1-beta-D-arabinofuranosylcytosine
-
wild-type, pH 7.5, 25C
0.007
1-beta-D-arabinofuranosylcytosine
-
wild type enzyme with ATP as cosubstrate
0.013
1-beta-D-arabinofuranosylcytosine
-
residues 65-79 deletion mutant with ATP as cosubstrate
0.024
1-beta-D-arabinofuranosylcytosine
-
mutant S74E, pH 7.5, 25C
0.0131
1-beta-D-arabinosylcytosine
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0506
1-beta-D-arabinosylcytosine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.1365
1-beta-D-arabinosylcytosine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0062
2',2'-difluorodeoxycytidine
-
wild-type, pH 7.5, 25C
0.0146
2',2'-difluorodeoxycytidine
-
wild-type
0.015
2',2'-difluorodeoxycytidine
-
mutant S74E, pH 7.5, 25C
0.15
2',3'-dideoxycytidine
-
cosubstrate UTP, 37C, pH 7.6
0.23
2',3'-dideoxycytidine
-
cosubstrate ATP, 37C, pH 7.6
0.48
2',3'-dideoxycytidine
-
cosubstrate UTP, 37C, pH 7.6
0.67
2',3'-dideoxycytidine
-
cosubstrate ATP, 37C, pH 7.6
0.0085
2'-deoxyadenosine
-
cosubstrate UTP, pH 7.5, 37C
0.013
2'-deoxyadenosine
-
wild type enzyme with UTP as cosubstrate
0.019
2'-deoxyadenosine
-
cosubstrate UTP, 37C, pH 7.6
0.053
2'-deoxyadenosine
-
residues 65-79 deletion mutant with UTP as cosubstrate
0.081
2'-deoxyadenosine
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
0.091
2'-deoxyadenosine
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
0.1
2'-deoxyadenosine
-
wild type enzyme with ATP as cosubstrate
0.1146
2'-deoxyadenosine
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.115
2'-deoxyadenosine
-
cosubstrate ATP, pH 7.5, 37C
0.1169
2'-deoxyadenosine
-
wild-type, pH 7.5, 25C
0.15
2'-deoxyadenosine
-
cosubstrate UTP, 37C, pH 7.6
0.1623
2'-deoxyadenosine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.231
2'-deoxyadenosine
-
mutant R104Q/D133G, pH 7.5, 30C
0.398
2'-deoxyadenosine
-
mutant R104M/D133S, pH 7.5, 37C
0.412
2'-deoxyadenosine
-
mutant R104M/D133A, pH 7.5, 37C
0.415
2'-deoxyadenosine
-
residues 65-79 deletion mutant with ATP as cosubstrate
0.452
2'-deoxyadenosine
-
mutant R104M/D133N, pH 7.5, 37C
0.48
2'-deoxyadenosine
-
cosubstrate ATP, 37C, pH 7.6
0.518
2'-deoxyadenosine
-
mutant S74E, pH 7.5, 25C
0.532
2'-deoxyadenosine
-
mutant R104Q/D133N, pH 7.5, 30C
0.55
2'-deoxyadenosine
-
cosubstrate ATP, 37C, pH 7.6
0.598
2'-deoxyadenosine
-
mutant A100V/R104M/D133A, pH 7.5, 37C
0.691
2'-deoxyadenosine
-
mutant R104M/D133T, pH 7.5, 37C
0.843
2'-deoxyadenosine
-
mutant A100V/R104M/D133S, pH 7.5, 37C
1.04
2'-deoxyadenosine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1.437
2'-deoxyadenosine
-
mutant A100V/R104M/D133T, pH 7.5, 37C
0.00005
2'-deoxycytidine
-
cosubstrate UTP, 37C, pH 7.6
0.0001
2'-deoxycytidine
-
cosubstrate UTP, 37C, pH 7.6
0.00016
2'-deoxycytidine
-
cosubstrate ATP, 37C, pH 7.6
0.00016
2'-deoxycytidine
-
with ATP as cosubstrate
0.00018
2'-deoxycytidine
-
cosubstrate ATP, 37C, pH 7.6
0.001
2'-deoxycytidine
-
pH 7.6, 37C, recombinant enzyme
0.001
2'-deoxycytidine
-
less than 0.001 mM, residues 65-79 deletion mutant with UTP as cosubstrate; less than 0.001 mM, wild type enzyme with ATP as cosubstrate; less than 0.001 mM, wild type enzyme with UTP as cosubstrate
0.001
2'-deoxycytidine
-
-
0.0014
2'-deoxycytidine
-
residues 65-79 deletion mutant with ATP as cosubstrate
0.0017
2'-deoxycytidine
-
wild-type, pH 7.5, 25C
0.003
2'-deoxycytidine
-
Km below 0.003 mM, wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0057
2'-deoxycytidine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.008
2'-deoxycytidine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0089
2'-deoxycytidine
-
mutant S74E, pH 7.5, 25C
0.021
2'-deoxyguanosine
-
wild type enzyme with UTP as cosubstrate
0.041
2'-deoxyguanosine
-
cosubstrate UTP, pH 7.5, 37C
0.079
2'-deoxyguanosine
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
0.081
2'-deoxyguanosine
-
mutant R104Q/D133G, pH 7.5, 30C
0.087
2'-deoxyguanosine
-
residues 65-79 deletion mutant with UTP as cosubstrate
0.154
2'-deoxyguanosine
-
wild-type, pH 7.5, 37C
0.155
2'-deoxyguanosine
-
wild-type, pH 7.5, 30C
0.181
2'-deoxyguanosine
-
cosubstrate ATP, pH 7.5, 37C
0.231
2'-deoxyguanosine
-
wild type enzyme with ATP as cosubstrate
0.231
2'-deoxyguanosine
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.24
2'-deoxyguanosine
-
mutant R104Q/D133N, pH 7.5, 30C
0.2458
2'-deoxyguanosine
-
wild-type, pH 7.5, 25C
0.324
2'-deoxyguanosine
-
mutant R104M/D133N, pH 7.5, 37C
0.474
2'-deoxyguanosine
-
residues 65-79 deletion mutant with ATP as cosubstrate
0.602
2'-deoxyguanosine
-
mutant S74E, pH 7.5, 25C
0.739
2'-deoxyguanosine
-
mutant A100V/R104M/D133S, pH 7.5, 37C
1.164
2'-deoxyguanosine
-
mutant A100V/R104M/D133T, pH 7.5, 37C
1.172
2'-deoxyguanosine
-
mutant R104M/D133S, pH 7.5, 37C
1.203
2'-deoxyguanosine
-
mutant R104M/D133T, pH 7.5, 37C
1.28
2'-deoxyguanosine
-
mutant R104M/D133A, pH 7.5, 37C
1.364
2'-deoxyguanosine
-
mutant A100V/R104M/D133A, pH 7.5, 37C
1.865
2'-deoxyguanosine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
2.266
2'-deoxyguanosine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0013
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
-
pH 7.6, 37C, recombinant enzyme
0.00099
2-chloro-2'-deoxyadenosine
-
cosubstrate UTP, 37C, pH 7.6
0.004
2-chloro-2'-deoxyadenosine
-
A119G mutant enzyme, 37C, pH 7.4; P122S mutant enzyme, 37C, pH 7.4
0.006
2-chloro-2'-deoxyadenosine
-
wild type enzyme, 37C, pH 7.4
0.0063
2-chloro-2'-deoxyadenosine
-
cosubstrate UTP, 37C, pH 7.6
0.009
2-chloro-2'-deoxyadenosine
-
I24V mutant enzyme, 37C, pH 7.4
0.017
2-chloro-2'-deoxyadenosine
-
cosubstrate ATP, 37C, pH 7.6
0.024
2-chloro-2'-deoxyadenosine
-
cosubstrate ATP, 37C, pH 7.6
0.0051
2-chlorodeoxyadenosine
-
cosubstrate UTP, pH 7.5, 37C
0.078
2-chlorodeoxyadenosine
-
cosubstrate ATP, pH 7.5, 37C
0.00032
4'-thio-2'-deoxycytidine
-
cosubstrate ATP 37C, pH 8.0
0.02
4'-thio-beta-D-arabinofuranosylcytosine
-
cosubstrate UTP 37C, pH 8.0
0.088
4'-thio-beta-D-arabinofuranosylcytosine
-
cosubstrate ATP 37C, pH 8.0
2
9-beta-D-arabinofuranosylguanine
-
cosubstrate UTP, 37C, pH 7.6
3
9-beta-D-arabinofuranosylguanine
-
cosubstrate ATP, 37C, pH 7.6
3
9-beta-D-arabinofuranosylguanine
-
cosubstrate UTP, 37C, pH 7.6
5
9-beta-D-arabinofuranosylguanine
-
cosubstrate ATP, 37C, pH 7.6
0.001
ATP
-
cosubstrate 2'-deoxycytidine, 37C, pH 7.4
0.0029
ATP
-
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.0034
ATP
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37C, pH 7.5
0.0035
ATP
-
wild type enzyme with 2'-deoxycytidine as cosubstrate
0.0084
ATP
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.0132
ATP
-
cosubstrate troxacitabine, wild-type, 37C, pH 7.5
0.019
ATP
-
wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.025
ATP
-
C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.053
ATP
-
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.0568
ATP
-
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.087
ATP
-
C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.093
ATP
-
wild type enzyme with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.097
ATP
-
wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.11
ATP
-
cosubstrate deoxycytidine, 37C, pH 8.0
0.2
ATP
-
cosubstrate cytosine arabinoside, 37C, pH 8.0
0.00041
beta-D-arabinofuranosylcytosine
-
cosubstrate UTP 37C, pH 8.0
0.015
beta-D-arabinofuranosylcytosine
-
cosubstrate ATP 37C, pH 8.0
0.00065
cladribine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.0016
cladribine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
0.0024
cladribine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.0155
cladribine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
0.0087
clofarabine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.0155
clofarabine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
0.022
clofarabine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.0325
clofarabine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
0.0013
CTP
-
cosubstrate 2'-deoxycytidine, 37C, pH 7.4
0.45
cytidine
-
37C, pH 7.0
1.4
cytidine
-
37C, pH 8.0
0.002
cytosine arabinoside
-
L-isomer, 25C, pH 7.6
0.002
cytosine arabinoside
-
deoxycytidine, cytosolic isozyme I, pH 7.5, 37C
0.007
cytosine arabinoside
-
cytosolic isozyme I, pH 7.5, 37C
0.02 - 0.04
cytosine arabinoside
-
37C, pH 8.0
0.02 - 0.04
cytosine arabinoside
-
D-isomer; pH 7.6, 25C
0.02 - 0.04
cytosine arabinoside
-
pH 7.0, 37C
0.02 - 0.04
cytosine arabinoside
-
pH 8.0, 37C
0.02 - 0.04
cytosine arabinoside
-
D-isomer
0.0243
D-thymidine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.144
D-thymidine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.12
deoxyadenosine
-
pH 8.0, 37C
0.15
deoxyadenosine
-
pH 8.0
0.5
deoxyadenosine
-
37C, pH 7.0
0.89
deoxyadenosine
-
37C, pH 7.5
0.00007
deoxycytidine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.00009
deoxycytidine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.00013
deoxycytidine
-
cosubstrate UTP 37C, pH 8.0
0.00018
deoxycytidine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
0.00027
deoxycytidine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
0.00057
deoxycytidine
-
cosubstrate ATP 37C, pH 8.0
0.0008 - 0.0015
deoxycytidine
-
37C, pH 7.0
0.0008 - 0.0015
deoxycytidine
-
pH 8.0, 37C
0.001
deoxycytidine
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
0.00104
deoxycytidine
-
mutant R104Q/D133G, pH 7.5, 30C
0.00141
deoxycytidine
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
0.00154
deoxycytidine
-
mutant A100V/R104M/D133A, pH 7.5, 37C
0.002
deoxycytidine
-
mutant R104M/D133A, pH 7.5, 37C
0.0033 - 0.0093
deoxycytidine
-
37C, pH 8.0
0.0033 - 0.0093
deoxycytidine
-
-
0.00455
deoxycytidine
-
mutant R104M/D133S, pH 7.5, 37C
0.00525
deoxycytidine
-
mutant A100V/R104M/D133S, pH 7.5, 37C
0.0086
deoxycytidine
-
mutant R104Q/D133N, pH 7.5, 30C
0.0118
deoxycytidine
-
mutant R104M/D133N, pH 7.5, 37C
0.014 - 0.0167
deoxycytidine
-
37C, pH 8.0
0.014 - 0.0167
deoxycytidine
-
37C, pH 8.0; cytosine arabinoside-induced cells, 37C, pH 8.0
0.014 - 0.0167
deoxycytidine
-
mitochondrial isozyme, pH 7.5, 37C; pH 7.5, 37C
0.014
deoxycytidine
-
37C, pH 8.0
0.0209
deoxycytidine
-
mutant R104M/D133T, pH 7.5, 37C
0.071
deoxycytidine
-
mutant A100V/R104M/D133T, pH 7.5, 37C
0.15
deoxyguanosine
-
pH 8.0, 37C
0.33 - 0.43
deoxyguanosine
-
-
0.33 - 0.43
deoxyguanosine
-
37C, pH 7.0
0.64
deoxyguanosine
-
37C, pH 7.5
3
deoxyguanosine
-
37C, pH 8.0
0.001
dTTP
-
cosubstrate 2'-deoxycytidine, 37C, pH 7.4
0.028
fludarabine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.142
fludarabine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
0.45
fludarabine
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.551
fludarabine
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
0.0161
gemcitabine
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0338
gemcitabine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0562
gemcitabine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.0009
GTP
-
cosubstrate 2'-deoxycytidine, 37C, pH 7.4
0.0178
L-thymidine
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.00388
thymidine
-
mutant A100V/R104M/D133T, pH 7.5, 37C
0.00558
thymidine
-
mutant A100V/R104M/D133S, pH 7.5, 37C
0.0058
thymidine
-
mutant R104M/D133T, pH 7.5, 37C
0.0189
thymidine
-
mutant R104M/D133S, pH 7.5, 37C
0.025
thymidine
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
0.0256
thymidine
-
mutant R104M/D133N, pH 7.5, 37C
0.0257
thymidine
-
mutant R104Q/D133G, pH 7.5, 30C
0.031
thymidine
-
mutant A100V/R104M/D133A, pH 7.5, 37C
0.036
thymidine
-
mutant R104Q/D133N, pH 7.5, 30C
0.074
thymidine
-
mutant R104M/D133A, pH 7.5, 37C
3.485
thymidine
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
0.011
troxacitabine
-
wild type enzyme with UTP as phosphate donor, 37C, pH 7.5
0.013
troxacitabine
-
wild type enzyme with ATP as phosphate donor, 37C, pH 7.5
0.057
troxacitabine
-
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37C, pH 7.5
0.058
troxacitabine
-
C9S/C45S/C59S/C146Smutant enzyme with UTP as phosphate donor, 37C, pH 7.5
0.0005
UTP
-
cosubstrate 2'-deoxycytidine, 37C, pH 7.4
0.0015
UTP
-
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.0023
UTP
-
wild type enzyme deletion mutant with 2'-deoxycytidine as cosubstrate
0.0047
UTP
-
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.008
UTP
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37C, pH 7.5
0.01
UTP
-
wild type enzyme deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.0106
UTP
-
cosubstrate troxacitabine, wild-type, 37C, pH 7.5
0.011
UTP
-
wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.0149
UTP
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.017
UTP
-
C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.02
UTP
-
C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.04
UTP
-
wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.0578
UTP
-
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.138
L-thymidine
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study; linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
-
additional information
additional information
-
Km-values of a variety of substrate derivatives
-
additional information
additional information
-
linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
Km-values of D-and L-cytidine analogues
-
additional information
additional information
-
Km-values of D-and L-analogues of cytidine and adenosine
-
additional information
additional information
-
no Michaelis-Menten kinetics, but negative cooperativity
-
additional information
additional information
-
steady-state fluorescent measurements, substrate binding constants, recombinant enzyme
-
additional information
additional information
-
kinetic properties of the enzyme are modulated in vivo by phosphorylation of Ser74, mechanism, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.028
(-)-beta-2',3'-dideoxy-3'-thiacytidine
Homo sapiens
-
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37C, pH 7.5
0.03
(-)-beta-2',3'-dideoxy-3'-thiacytidine
Homo sapiens
-
wild type enzyme with ATP as phosphate donor, 37C, pH 7.5
0.072
(-)-beta-2',3'-dideoxy-3'-thiacytidine
Homo sapiens
-
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37C, pH 7.5
0.102
(-)-beta-2',3'-dideoxy-3'-thiacytidine
Homo sapiens
-
wild type enzyme with UTP as phosphate donor, 37C, pH 7.5
0.15
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
residues 65-79 deletion mutant with UTP as cosubstrate
0.17
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
wild type enzyme with UTP as cosubstrate
0.3
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
wild type enzyme with ATP as cosubstrate
0.3
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
mutant S74Q, pH 7.5, 25C
0.32
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
wild-type, pH 7.5, 25C
0.33
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
mutant S74A, pH 7.5, 25C
0.41
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
residues 65-79 deletion mutant with ATP as cosubstrate
0.57
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
mutant S74D, pH 7.5, 25C
1.06
1-beta-D-arabinofuranosylcytosine
Homo sapiens
-
mutant S74E, pH 7.5, 25C
0.34
1-beta-D-arabinosylcytosine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.79
1-beta-D-arabinosylcytosine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1.43
1-beta-D-arabinosylcytosine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.44
2',2'-difluorodeoxycytidine
Homo sapiens
-
wild-type, pH 7.5, 25C
0.45
2',2'-difluorodeoxycytidine
Homo sapiens
-
mutant S74Q, pH 7.5, 25C
0.5
2',2'-difluorodeoxycytidine
Homo sapiens
-
mutant S74A, pH 7.5, 25C
0.9
2',2'-difluorodeoxycytidine
Homo sapiens
-
mutant S74D, pH 7.5, 25C
1.21
2',2'-difluorodeoxycytidine
Homo sapiens
-
mutant S74E, pH 7.5, 25C
0.31
2'-deoxyadenosine
Homo sapiens
-
residues 65-79 deletion mutant with UTP as cosubstrate
0.33
2'-deoxyadenosine
Homo sapiens
-
wild type enzyme with UTP as cosubstrate
0.37
2'-deoxyadenosine
Homo sapiens
-
cosubstrate UTP, pH 7.5, 37C
0.69
2'-deoxyadenosine
Homo sapiens
-
mutant S74E, pH 7.5, 25C
0.77
2'-deoxyadenosine
Homo sapiens
-
wild-type, pH 7.5, 25C
0.9
2'-deoxyadenosine
Homo sapiens
-
mutant S74Q, pH 7.5, 25C
1
2'-deoxyadenosine
Homo sapiens
-
mutant S74D, pH 7.5, 25C
1.08
2'-deoxyadenosine
Homo sapiens
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
1.12
2'-deoxyadenosine
Homo sapiens
-
mutant S74A, pH 7.5, 25C
1.7
2'-deoxyadenosine
Homo sapiens
-
wild type enzyme with ATP as cosubstrate
1.75
2'-deoxyadenosine
Homo sapiens
-
mutant R104Q/D133G, pH 7.5, 30C
2
2'-deoxyadenosine
Homo sapiens
-
mutant R104Q/D133N, pH 7.5, 30C
2.1
2'-deoxyadenosine
Homo sapiens
-
cosubstrate ATP, pH 7.5, 37C
2.13
2'-deoxyadenosine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
2.67
2'-deoxyadenosine
Homo sapiens
-
mutant A100V/R104M/D133T, pH 7.5, 37C
2.71
2'-deoxyadenosine
Homo sapiens
-
mutant R104M/D133A, pH 7.5, 37C
2.8
2'-deoxyadenosine
Homo sapiens
-
residues 65-79 deletion mutant with ATP as cosubstrate
3.1
2'-deoxyadenosine
Homo sapiens
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
3.37
2'-deoxyadenosine
Homo sapiens
-
mutant A100V/R104M/D133S, pH 7.5, 37C
3.4
2'-deoxyadenosine
Homo sapiens
-
mutant A100V/R104M/D133A, pH 7.5, 37C
3.47
2'-deoxyadenosine
Homo sapiens
-
mutant R104M/D133N, pH 7.5, 37C
4.14
2'-deoxyadenosine
Homo sapiens
-
mutant R104M/D133T, pH 7.5, 37C
4.51
2'-deoxyadenosine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
4.85
2'-deoxyadenosine
Homo sapiens
-
mutant R104M/D133S, pH 7.5, 37C
5.72
2'-deoxyadenosine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.033
2'-deoxycytidine
Homo sapiens
-
less than 0.001 mM, wild type enzyme with ATP as cosubstrate
0.04
2'-deoxycytidine
Homo sapiens
-
mutant S74A, pH 7.5, 25C; mutant S74Q, pH 7.5, 25C; wild-type, pH 7.5, 25C
0.04
2'-deoxycytidine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.044
2'-deoxycytidine
Homo sapiens
-
less than 0.001 mM, wild type enzyme with UTP as cosubstrate
0.049
2'-deoxycytidine
Homo sapiens
-
residues 65-79 deletion mutant with ATP as cosubstrate
0.088
2'-deoxycytidine
Homo sapiens
-
less than 0.001 mM, residues 65-79 deletion mutant with UTP as cosubstrate
0.11
2'-deoxycytidine
Homo sapiens
-
pH 7.6, 37C, recombinant enzyme
0.16
2'-deoxycytidine
Homo sapiens
-
mutant S74D, pH 7.5, 25C
0.25
2'-deoxycytidine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.45
2'-deoxycytidine
Homo sapiens
-
mutant S74E, pH 7.5, 25C
1.8
2'-deoxycytidine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.31
2'-deoxyguanosine
Homo sapiens
-
mutant A100V/R104M/D133A, pH 7.5, 37C
0.32
2'-deoxyguanosine
Homo sapiens
-
residues 65-79 deletion mutant with UTP as cosubstrate
0.33
2'-deoxyguanosine
Homo sapiens
-
wild type enzyme with UTP as cosubstrate
0.36
2'-deoxyguanosine
Homo sapiens
-
mutant S74E, pH 7.5, 25C
0.53
2'-deoxyguanosine
Homo sapiens
-
cosubstrate UTP, pH 7.5, 37C
0.57
2'-deoxyguanosine
Homo sapiens
-
mutant S74D, pH 7.5, 25C
0.65
2'-deoxyguanosine
Homo sapiens
-
mutant S74Q, pH 7.5, 25C
0.68
2'-deoxyguanosine
Homo sapiens
-
mutant S74A, pH 7.5, 25C
0.73
2'-deoxyguanosine
Homo sapiens
-
wild-type, pH 7.5, 25C
1.18
2'-deoxyguanosine
Homo sapiens
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
1.23
2'-deoxyguanosine
Homo sapiens
-
mutant R104M/D133A, pH 7.5, 37C
1.5
2'-deoxyguanosine
Homo sapiens
-
mutant R104Q/D133G, pH 7.5, 30C
1.72
2'-deoxyguanosine
Homo sapiens
-
mutant A100V/R104M/D133S, pH 7.5, 37C
1.73
2'-deoxyguanosine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1.92
2'-deoxyguanosine
Homo sapiens
-
mutant R104Q/D133N, pH 7.5, 30C
2.2
2'-deoxyguanosine
Homo sapiens
-
residues 65-79 deletion mutant with ATP as cosubstrate
2.29
2'-deoxyguanosine
Homo sapiens
-
mutant A100V/R104M/D133T, pH 7.5, 37C
2.5
2'-deoxyguanosine
Homo sapiens
-
mutant R104M/D133T, pH 7.5, 37C
2.5
2'-deoxyguanosine
Homo sapiens
-
cosubstrate ATP, pH 7.5, 37C
2.6
2'-deoxyguanosine
Homo sapiens
-
wild type enzyme with ATP as cosubstrate
2.6
2'-deoxyguanosine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
3.26
2'-deoxyguanosine
Homo sapiens
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
3.59
2'-deoxyguanosine
Homo sapiens
-
mutant R104M/D133N, pH 7.5, 37C
3.66
2'-deoxyguanosine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
4.38
2'-deoxyguanosine
Homo sapiens
-
mutant R104M/D133S, pH 7.5, 37C
0.02
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
Homo sapiens
-
pH 7.6, 37C, recombinant enzyme
0.37
2-chlorodeoxyadenosine
Homo sapiens
-
cosubstrate UTP, pH 7.5, 37C
0.6
2-chlorodeoxyadenosine
Homo sapiens
-
cosubstrate ATP, pH 7.5, 37C
0.012
ATP
Homo sapiens
-
cosubstrate L-2'-deoxycytidine, wild-type, 37C, pH 7.5
0.028
ATP
Homo sapiens
-
wild type enzyme with 2'-deoxycytidine as cosubstrate
0.028
ATP
Homo sapiens
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.03
ATP
Homo sapiens
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37C, pH 7.5
0.033
ATP
Homo sapiens
-
cosubstrate D-2'-deoxycytidine, wild-type, 37C, pH 7.5
0.036
ATP
Homo sapiens
-
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.039
ATP
Homo sapiens
-
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.095
ATP
Homo sapiens
-
cosubstrate troxacitabine, wild-type, 37C, pH 7.5
0.173
ATP
Homo sapiens
-
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.192
ATP
Homo sapiens
-
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.36
ATP
Homo sapiens
-
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.4
ATP
Homo sapiens
-
wild type enzyme with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.0804
cladribine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.172
cladribine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.211
cladribine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
1.386
cladribine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
0.486
clofarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.88
clofarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
1.11
clofarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
2.45
clofarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
1.74
D-thymidine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
3.2
D-thymidine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.015
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.021
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.064
deoxycytidine
Homo sapiens
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
0.123
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
0.183
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
0.21
deoxycytidine
Homo sapiens
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
0.37
deoxycytidine
Homo sapiens
-
mutant R104Q/D133G, pH 7.5, 30C
0.58
deoxycytidine
Homo sapiens
-
mutant R104M/D133A, pH 7.5, 37C
1.2
deoxycytidine
Homo sapiens
-
mutant A100V/R104M/D133A, pH 7.5, 37C
1.3
deoxycytidine
Homo sapiens
-
mutant R104Q/D133N, pH 7.5, 30C
1.7
deoxycytidine
Homo sapiens
-
mutant R104M/D133S, pH 7.5, 37C
2.1
deoxycytidine
Homo sapiens
-
mutant A100V/R104M/D133S, pH 7.5, 37C
2.23
deoxycytidine
Homo sapiens
-
mutant R104M/D133N, pH 7.5, 37C
2.86
deoxycytidine
Homo sapiens
-
mutant R104M/D133T, pH 7.5, 37C
3.54
deoxycytidine
Homo sapiens
-
mutant A100V/R104M/D133T, pH 7.5, 37C
1330
deoxycytidine
Mus musculus
-
cytosine arabinoside-induced cells
78300
deoxycytidine
Mus musculus
-
-
0.177
fludarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
0.363
fludarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
1.005
fludarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
1.334
fludarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
0.39
gemcitabine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
2.01
gemcitabine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
2.68
gemcitabine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1.33
L-thymidine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
3.13
L-thymidine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
0.38
thymidine
Homo sapiens
-
wild-type, pH 7.5, 30C; wild-type, pH 7.5, 37C
0.68
thymidine
Homo sapiens
-
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30C
1.01
thymidine
Homo sapiens
-
mutant R104Q/D133G, pH 7.5, 30C
1.49
thymidine
Homo sapiens
-
mutant R104M/D133T, pH 7.5, 37C
1.51
thymidine
Homo sapiens
-
mutant R104Q/D133N, pH 7.5, 30C
1.57
thymidine
Homo sapiens
-
mutant A100V/R104M/D133T, pH 7.5, 37C
1.88
thymidine
Homo sapiens
-
mutant R104M/D133A, pH 7.5, 37C
1.91
thymidine
Homo sapiens
-
mutant A100V/R104M/D133S, pH 7.5, 37C
2.27
thymidine
Homo sapiens
-
mutant R104M/D133N, pH 7.5, 37C
2.33
thymidine
Homo sapiens
-
mutant R104M/D133S, pH 7.5, 37C
3.29
thymidine
Homo sapiens
-
mutant A100V/R104M/D133A, pH 7.5, 37C
0.095
troxacitabine
Homo sapiens
-
wild type enzyme with ATP as phosphate donor, 37C, pH 7.5
0.192
troxacitabine
Homo sapiens
-
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37C, pH 7.5
6.08
troxacitabine
Homo sapiens
-
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37C, pH 7.5
0.01
UTP
Homo sapiens
-
cosubstrate L-2'-deoxycytidine, wild-type, 37C, pH 7.5
0.031
UTP
Homo sapiens
-
wild type enzyme deletion mutant with 2'-deoxycytidine as cosubstrate
0.041
UTP
Homo sapiens
-
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.042
UTP
Homo sapiens
-
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.049
UTP
Homo sapiens
-
cosubstrate D-2'-deoxycytidine, wild-type, 37C, pH 7.5
0.072
UTP
Homo sapiens
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.075
UTP
Homo sapiens
-
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.093
UTP
Homo sapiens
-
wild type enzyme deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.102
UTP
Homo sapiens
-
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37C, pH 7.5
0.179
UTP
Homo sapiens
-
cosubstrate troxacitabine, wild-type, 37C, pH 7.5
0.217
UTP
Homo sapiens
-
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
0.524
UTP
Homo sapiens
-
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37C, pH 7.5
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10.5
1-beta-D-arabinosylcytosine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
8478
15.6
1-beta-D-arabinosylcytosine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
8478
26
1-beta-D-arabinosylcytosine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
8478
4.3
2'-deoxyadenosine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
357
18.6
2'-deoxyadenosine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
357
35.2
2'-deoxyadenosine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
357
13.3
2'-deoxycytidine
Homo sapiens
-
kcat/Km above 13.3/sec*mM, wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
486
31.3
2'-deoxycytidine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
486
315.8
2'-deoxycytidine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
486
0.9
2'-deoxyguanosine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
504
1.6
2'-deoxyguanosine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
504
11.3
2'-deoxyguanosine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
504
74.3
cladribine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
5796
90.2
cladribine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
5796
130
cladribine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
5796
136
cladribine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
5796
50.1
clofarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
5855
56.3
clofarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
5855
57.6
clofarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
5855
76.8
clofarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
5855
12.1
D-thymidine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
14935
131.7
D-thymidine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
14935
236
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
451
257
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
451
676
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
451
745
deoxycytidine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
451
1.8
fludarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate ATP
4150
2.6
fludarabine
Homo sapiens
-
pH 7.6, 37C, enzyme mutant S74E, with co-substrate UTP
4150
3
fludarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate ATP
4150
6.4
fludarabine
Homo sapiens
-
pH 7.6, 37C, wild-type enzyme, with co-substrate UTP
4150
24.2
gemcitabine
Homo sapiens
-
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1899
47.7
gemcitabine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1899
59.5
gemcitabine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
1899
22.7
L-thymidine
Homo sapiens
-
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
14107
74.7
L-thymidine
Homo sapiens
-
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37C
14107
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
2'-O-methylcytidine
-
37C, pH 7.6
0.02
3'-O-methyl-2'deoxycytidine
-
37C, pH 7.6
0.032
5'-O-ethyl-2'-deoxycytidine
-
37C, pH 7.6
0.23
5'-O-methyl-2'-deoxycytidine
-
37C, pH 7.6
0.0015
dCMP
-
cosubstrate UTP, 37C, pH 7.4
0.022
dCMP
-
cosubstrate deoxycytidine, 37C, pH 7.4
0.0015
dCTP
-
pH 7.6, 37C, enzyme mutant S74E, with substrates cladribine and ATP, mixed inhibition
0.0029
dCTP
-
pH 7.6, 37C, enzyme mutant S74E, with substrates deoxycytidine and ATP, mixed inhibition
0.0058
dCTP
-
pH 7.6, 37C, wild-type enzyme, with substrates cladribine and ATP, mixed inhibition
0.027
dCTP
-
pH 7.6, 37C, enzyme mutant S74E, with substrates cladribine and UTP, uncompetitive inhibition
0.03
dCTP
-
pH 7.6, 37C, wild-type enzyme, with substrates deoxycytidine and ATP, mixed inhibition
0.05
dCTP
-
pH 7.6, 37C, wild-type enzyme, with substrates cladribine and UTP, uncompetitive inhibition
0.052
dCTP
-
pH 7.6, 37C, enzyme mutant S74E, with substrates deoxycytidine and UTP, uncompetitive inhibition
0.0004
UDP
-
cosubstrate UTP, 37C, pH 7.4
0.0069
UDP
-
cosubstrate deoxycytidine, 37C, pH 7.4
0.398
dCTP
-
pH 7.6, 37C, wild-type enzyme, with substrates deoxycytidine and UTP, uncompetitive inhibition
additional information
additional information
-
steady-state fluorescent measurement, inhibitor binding constant, recombinant enzyme
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00012
2'-deoxy-5-fluorocytidine
Mus musculus
-
-
0.000003
2-(2-[1-[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]piperidin-4-yl]ethoxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000034
2-([1-[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]piperidin-4-yl]methoxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000038
2-([1-[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000003
2-([1-[6-(2-chlorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.00001
2-([1-[6-(2-ethylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000022
2-([1-[6-(3-chlorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000009
2-([1-[6-(4-chloro-2-methylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000019
2-([1-[6-(4-chlorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000003
2-([1-[6-(biphenyl-2-yloxy)pyrimidin-4-yl]piperidin-4-yl]oxy)-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000063
2-([6-[4-(4-amino-2-oxopyrimidin-1(2H)-yl)piperidin-1-yl]-5-fluoropyrimidin-4-yl]oxy)benzonitrile
Mus musculus
-
-
0.000005
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-2-bromopyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.00001
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-2-methylpyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.000002
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]-5-methoxybenzonitrile
Mus musculus
-
-
0.000001
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.000021
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-methylpyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.000003
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]pyrimidin-4-yl)oxy]-5-methoxybenzonitrile
Mus musculus
-
-
0.000003
2-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]pyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.0000007
2-[(6-[4-[(4-aminopyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.00072
2-[[6-(1-benzothiophen-2-yl)pyrimidin-4-yl]oxy]-5-fluoropyrimidin-4-amine
Mus musculus
-
-
0.000089
3'-[[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]sulfamoyl]biphenyl-4-carboxamide
Homo sapiens
-
-
0.000001
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-2-fluoropyrimidin-4-yl)oxy]-4-methoxybenzonitrile
Mus musculus
-
-
0.000005
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]-4-methoxybenzonitrile
Mus musculus
-
-
0.000004
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]-5-fluoropyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.000013
3-[(6-[4-[(4-amino-5-fluoropyrimidin-2-yl)oxy]piperidin-1-yl]pyrimidin-4-yl)oxy]benzonitrile
Mus musculus
-
-
0.000024
4'-thio-beta-D-arabinofuranosylcytosine
Homo sapiens
-
IC50 0.000024 mM
0.00051
4-(1-benzothiophen-2-yl)-6-[4-(2-oxo-2-pyrrolidin-1-ylethyl)piperazin-1-yl]pyrimidine
Mus musculus
-
-
0.00051
4-(1-benzothiophen-2-yl)-6-[4-(2-oxo-2-pyrrolidin-1-ylethyl)piperazin-1-yl]pyrimidine
Homo sapiens
-
-
0.051
5-chloro-4'-thio-beta-D-arabinofuranosylcytosine
Homo sapiens
-
IC50 0.051 mM
0.00012
5-Fluoro-2'-deoxycytidine
Homo sapiens
-
-
0.000004
5-fluoro-2-([1-[5-fluoro-6-(4-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000006
5-fluoro-2-([1-[6-(2-fluorophenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000005
5-fluoro-2-([1-[6-(2-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000009
5-fluoro-2-([1-[6-(2-methylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000023
5-fluoro-2-([1-[6-(3-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000033
5-fluoro-2-([1-[6-(4-methoxyphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000021
5-fluoro-2-([1-[6-(4-methylphenoxy)pyrimidin-4-yl]piperidin-4-yl]oxy)pyrimidin-4-amine
Mus musculus
-
-
0.000095
5-fluoro-2-[[1-(6-phenoxypyrimidin-4-yl)piperidin-4-yl]oxy]pyrimidin-4-amine
Mus musculus
-
-
0.000002
5-fluoro-4'-thio-beta-D-arabinofuranosylcytosine
Homo sapiens
-
IC50 0.0000020 mM
0.0000055
cytosine arabinoside
Homo sapiens
-
IC50 0.0000055 mM
0.0138
LP-375752
Homo sapiens
-
in 50 mM Tris (pH 7.6)
0.00091
LP-503392
Homo sapiens
-
in 50 mM Tris (pH 7.6)
0.000021
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-2',4'-dichlorobiphenyl-3-sulfonamide
Homo sapiens
-
-
0.000045
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-2'-chlorobiphenyl-3-sulfonamide
Homo sapiens
-
-
0.000023
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3'-chlorobiphenyl-3-carboxamide
Homo sapiens
-
-
0.000079
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3'-chlorobiphenyl-3-sulfonamide
Homo sapiens
-
-
0.00035
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3-bromobenzenesulfonamide
Homo sapiens
-
-
0.0019
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-3-bromobenzenesulfonamide
Homo sapiens
-
-
0.000021
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4''-chloro-2''-methyl-1,1':4',1''-terphenyl-3-carboxamide
Homo sapiens
-
-
0.000021
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4''-chloro-2''-methyl-1,1':4',1''-terphenyl-3-sulfonamide
Homo sapiens
-
-
0.000063
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-(methylsulfonyl)biphenyl-3-sulfonamide
Homo sapiens
-
-
0.000051
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-chlorobiphenyl-3-sulfonamide
Homo sapiens
-
-
0.000049
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-cyanobiphenyl-3-sulfonamide
Homo sapiens
-
-
0.0153
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-formylbiphenyl-3-sulfonamide
Homo sapiens
-
-
0.00003
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methoxybiphenyl-3-carboxamide
Homo sapiens
-
-
0.000042
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methoxybiphenyl-3-carboxamide
Homo sapiens
-
-
0.000064
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methoxybiphenyl-3-sulfonamide
Homo sapiens
-
-
0.00011
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4'-methylbiphenyl-3-sulfonamide
Homo sapiens
-
-
0.0000017
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4-(1-benzothiophen-2-yl)pyridine-2-carboxamide
Homo sapiens
-
-
0.0027
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4-bromobenzenesulfonamide
Homo sapiens
-
-
0.0032
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]-4-nitrobenzenesulfonamide
Homo sapiens
-
-
0.0036
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]benzenesulfonamide
Homo sapiens
-
-
0.00026
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]biphenyl-3-carboxamide
Homo sapiens
-
-
0.00012
N-[(1S,3S)-3-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)cyclopentyl]biphenyl-3-sulfonamide
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0000057
-
differentiated adipocytes
0.0000521
-
CEM lymphocytes
0.00088
-
cytosine arabinoside-induced cells
0.0048
-
37C, pH 7.0
0.00523
-
37C, pH 8.0
0.00632
-
37C, pH 8.0
0.012
-
37C, pH 8.0
0.0206
-
37C, pH 8.0
0.048
-
mitochondrial isozyme, pH 7.5, 37C
0.072
-
cytoplasmic isozyme I, pH 7.5, 37C
0.0846
-
37C, pH 8.0
0.11 - 0.15
-
pH 8.0, 37C
4
-
cytidine as substrate, 37C, pH 7.0
6.5
-
substrate dideoxythymidine, wild-type, pH 7.5, 37C
7.2
-
deoxyguanosine as substrate, 37C, pH 7.0
8
-
37C, pH 7.0
13.5
-
deoxyadenosine as substrate, 37C, pH 7.0
26
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133T, pH 7.5, 37C
28
-
substrate dideoxythymidine, mutant R104M/D133S, pH 7.5, 37C
64
-
substrate dideoxythymidine, mutant A100V/R104M/D133A, pH 7.5, 37C
88
-
substrate 2'-deoxy-3'-thiacytidine, wild-type, pH 7.5, 37C
108
-
substrate dideoxythymidine, mutant R104M/D133T, pH 7.5, 37C
114
-
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133T, pH 7.5, 37C
135
-
substrate dideoxythymidine, mutant A100V/R104M/D133T, pH 7.5, 37C
149
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133A, pH 7.5, 37C
186
-
substrate dideoxythymidine, mutant A100V/R104M/D133S, pH 7.5, 37C
206
-
substrate dideoxythymidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37C
270
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133S, pH 7.5, 37C
545
-
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133S, pH 7.5, 37C
599
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133T, pH 7.5, 37C
815
-
substrate 2'-deoxy-3'-thiacytidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37C
984
-
substrate 1-beta-D-arabinosylcytosine, wild-type, pH 7.5, 37C
1387
-
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133T, pH 7.5, 37C
1648
-
substrate gemcitabine, wild-type, pH 7.5, 37C
2262
-
substrate gemcitabine, mutant A100V/R104M/D133T, pH 7.5, 37C
3177
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133S, pH 7.5, 37C
3308
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133A, pH 7.5, 37C
3339
-
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133SA, pH 7.5, 37C
4267
-
substrate gemcitabine, mutant R104M/D133T, pH 7.5, 37C
4400
-
substrate 1-beta-D-arabinosylcytosine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37C
4530
-
substrate gemcitabine, mutant A100V/R104M/D133A, pH 7.5, 37C
5624
-
substrate gemcitabine, mutant R104M/D133SA, pH 7.5, 37C
6009
-
substrate gemcitabine, mutant A100V/R104M/D133S, pH 7.5, 37C
6460
-
substrate gemcitabine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37C
additional information
-
-
additional information
-
substrate specificity
additional information
-
MTT assay method
additional information
-
catalytic efficiencies of the purified recombinant enzyme
additional information
-
relative enzyme activity in different cancer cell lines, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 7
-
broad
5.5 - 8.5
-
plateau, higher activity in Tris than in phosphate buffer
6 - 10
-
broad plateau, with very slight optima at pH 6 and 10
6 - 10
-
nearly flat response to pH-variations
7
-
broad
7.4
-
assay at
7.5
-
assay at
7.6
-
assay at
7.6
-
assay at
8
-
aassay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 10
-
maximal activity at pH 5 and about half-maximal activity at pH 10
5.8 - 10
-
about half-maximal activity at pH 5.8 and pH 10, about 90% of maximal activity at pH 6.5 and pH 9
7 - 10
-
about 80% of maximal activity at pH 7 and about 65% of maximal activity at pH 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
25
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
27 - 37
-
moderately sensitive to variations of incubation temperature
37 - 55
-
about half-maximal activity at 37C and 55C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
mononuclear cells used for experiments
Manually annotated by BRENDA team
-
low enzyme expression level
Manually annotated by BRENDA team
-
lowest specific activity among the cell lines used
Manually annotated by BRENDA team
-
P815-neoplasm cell line
Manually annotated by BRENDA team
-
enzyme activity increases up to 3fold when cells start to proliferate
Manually annotated by BRENDA team
-
proliferating lymphocyte
Manually annotated by BRENDA team
-
highest specific activity among the cell lines used
Manually annotated by BRENDA team
-
; low enzyme activity
Manually annotated by BRENDA team
-
enzyme activity increases up to 3fold when cells start to proliferate
Manually annotated by BRENDA team
-
very low enzyme expression
Manually annotated by BRENDA team
-
from acute myeloid leukemia (AML) patients; from peripheral blood; ML-1 cells
Manually annotated by BRENDA team
-
from spleen; leukemic T-lymphoblasts
Manually annotated by BRENDA team
-
leukemic T-lymphoblasts
Manually annotated by BRENDA team
-
cell line MOLT-4; leukemic T-lymphoblasts
Manually annotated by BRENDA team
-
from acute myeloid leukemia (AML) patients
Manually annotated by BRENDA team
-
various spontaneous leukemic cells
Manually annotated by BRENDA team
-
cell line MOLT-4
Manually annotated by BRENDA team
-
lymphomic
Manually annotated by BRENDA team
-
very low enzyme expression
Manually annotated by BRENDA team
-
high enzyme content in fetal liver, very low content in adult liver
Manually annotated by BRENDA team
-
adult non-small cell lung cancer
Manually annotated by BRENDA team
-
normal and leukemic
Manually annotated by BRENDA team
-
T-lymphoblasts, cell lines MOLT 4F, CCRF, CEM and RPMI 8402, B-lymphoblasts cell lines BALL 1 and EBV (Epstein-Barr-virus) transformed B-lymphoblasts
Manually annotated by BRENDA team
-
from human leukemia patients
Manually annotated by BRENDA team
-
normal, acute myeloid leukemic and HL60 promyelocytic cells
Manually annotated by BRENDA team
-
normal cells and various leukemic cell lines
Manually annotated by BRENDA team
-
tonsillar lymphocyte
Manually annotated by BRENDA team
-
B-cell chronic lymphocytic leukaemia lymphocyte
Manually annotated by BRENDA team
-
high enzyme expression
Manually annotated by BRENDA team
-
enzyme activity increases up to 3fold when cells start to proliferate
Manually annotated by BRENDA team
-
activity increases within 36 h of growth compared to resting cells
Manually annotated by BRENDA team
-
very low enzyme expression
Manually annotated by BRENDA team
-
very low enzyme expression
Manually annotated by BRENDA team
-
pancreatic cancer tissue, enzyme detected in 40 out of 44 patient samples
Manually annotated by BRENDA team
-
gemcitabine-resistant pancreatic cancer cell
Manually annotated by BRENDA team
-
deoxycytidine kinase activty is about 10fold lower compared with other tissues
Manually annotated by BRENDA team
-
; low enzyme activity; vastus
Manually annotated by BRENDA team
-
cells irradiated in the late exponential growth phase for experiments
Manually annotated by BRENDA team
-
; suspension culture
Manually annotated by BRENDA team
-
lymphocyte
Manually annotated by BRENDA team
additional information
-
very low activity in normal spleens and placentas
Manually annotated by BRENDA team
additional information
-
tissue specific enzyme expression
Manually annotated by BRENDA team
additional information
-
enzyme level in case of acute myeloid leukemia and acute lymphoblastic leukemia in 97 patients, expression levels in different cancer cells, overview
Manually annotated by BRENDA team
additional information
-
high enzyme expression level in case of acute myeloid leukemia and acute lymphoblastic leukemia, sensitivity of cell types to cytarabine, overview
Manually annotated by BRENDA team
additional information
-
enzyme expression throughout the cell cycle, tissue-specific expression
Manually annotated by BRENDA team
additional information
-
expression of mRNA in all tissues examined, with 5- to 10fold variation
Manually annotated by BRENDA team
additional information
-
enzyme activity tissue distribution analysis, overview
Manually annotated by BRENDA team
additional information
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dCK-negative L1210-10K tumors
Manually annotated by BRENDA team
additional information
Mus musculus C57BL/6J
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dCK-negative L1210-10K tumors
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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2 isozymes: I and II
Manually annotated by BRENDA team
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native enzyme, perinuclear and cellular membrane area
Manually annotated by BRENDA team
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native enzyme
Manually annotated by BRENDA team
Mus musculus C57BL/6J
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-
-
Manually annotated by BRENDA team
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only when overexpressed
Manually annotated by BRENDA team
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enzyme possesses a nuclear localization signal at the N-terminus
Manually annotated by BRENDA team
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main localization of both wild-type and mutant S74E mimicking phosphorylated enzyme
Manually annotated by BRENDA team
additional information
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subcellular distribution
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Manually annotated by BRENDA team
additional information
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the enzyme possesses a nuclear import signal sequence
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Manually annotated by BRENDA team