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Information on EC 2.7.1.67 - 1-phosphatidylinositol 4-kinase and Organism(s) Rattus norvegicus
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2.7.1.67 1-phosphatidylinositol 4-kinaseIUBMB Comments
This reaction is catalysed by at least two different isoforms.
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Word Map
- 2.7.1.67
- 4-phosphate
- 4,5-bisphosphate
- phospholipid
- phospholipase
-
ptdins4p
- wortmannin
- phosphatidylinositol-4-phosphate
- pip2
- 5-kinase
- diacylglycerol
-
polyphosphoinositides
-
ptdins4,5p2
-
phosphatidic
-
trans-golgi
-
gamma-32patp
-
1,4,5-trisphosphate
- phosphatidylinositol-4,5-bisphosphate
-
polyoma
- coxsackievirus
-
wortmannin-sensitive
-
pleckstrin
-
antiphosphotyrosine
-
oxysterol-binding
-
positive-sense
- enteroviruses
-
3hinositol
-
ovcar-5
- sensor-1
- ns5a
- poliovirus
- medicine
- pharmacology
- drug development
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
phosphatidylinositol kinase, pi kinase, pi4kb, pi 4-kinase, ptdins 4-kinase, pi4kiialpha, pi4ka, pi4kbeta, phosphatidylinositol-4-kinase, pik1p, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kinase, phosphatidylinositol (phosphorylating)
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-
-
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phosphatidylinositol 4-kinase
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-
-
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phosphatidylinositol kinase
-
-
-
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PI 4-kinase
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-
-
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PI kinase
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-
-
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PI4K
-
-
-
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PtdIns 4-kinase
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-
-
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type II phosphatidylinositol kinase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1-phosphatidyl-1D-myo-inositol 4-phosphotransferase
This reaction is catalysed by at least two different isoforms.
CAS REGISTRY NUMBER
COMMENTARY
37205-54-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GTP + 1-phosphatidyl-1D-myo-inositol
GDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
no activity
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
641769, 641770, 641773, 641789, 641790, 641796, 641802, 641806, 641807, 641812, 641817, 641820, 662444, 662885, 673232
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
PI4K92 is a key enzyme regulating Golgi disintegration/reorganization during mitosis probably via phosphorylation by cyclin-dependent kinases on well-defined sites. PI4K55 is involved in the production of second messengers, diacylglycerol and inositol 1,4,5-triphosphate at the plasma membrane, moreover, in the endocytotic pathway in the cytoplasm
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
the enzyme is an integral component of the early signal transduction machinery during T-cell activation by concanavalin A and is actively regulated by protein tyrosine phosphorylation-dephosphorylation
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
generation of 1-phosphatidyl-1D-myo-inositol 4-phosphate by the enzyme on synaptic vesicles may be the first step in the resynthesis of phosphatidylinositol 4,5-bisphosphate
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
phosphatidylinositol 4-kinase is a component of glucose transporter-containing vesicles. It may play a role in defining the fusogenic properties necessary to mediate membrane movement between the glucose transporter vesicles, plasma membranes and micosomes
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
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integral componant of FcepsilonRI mediated signal transduction mechanism
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
neuronal calcium sensor-1 and the GTPase ARF1 are localized to the Golgi complex and regulate phosphatidylinositol 4-kinase IIIbeta. Spatial and temporal control of phosphatidylinositol 4-phosphate levels through activation of phosphatidylinositol 4-kinase IIIbeta is important for the recruitment of trafficking complexes to the trans-Golgi network and vesicular traffic from this organelle
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
-
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
PI4K92 is a key enzyme regulating Golgi disintegration/reorganization during mitosis probably via phosphorylation by cyclin-dependent kinases on well-defined sites. PI4K55 is involved in the production of second messengers, diacylglycerol and inositol 1,4,5-triphosphate at the plasma membrane, moreover, in the endocytotic pathway in the cytoplasm
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
the enzyme is an integral component of the early signal transduction machinery during T-cell activation by concanavalin A and is actively regulated by protein tyrosine phosphorylation-dephosphorylation
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
generation of 1-phosphatidyl-1D-myo-inositol 4-phosphate by the enzyme on synaptic vesicles may be the first step in the resynthesis of phosphatidylinositol 4,5-bisphosphate
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
phosphatidylinositol 4-kinase is a component of glucose transporter-containing vesicles. It may play a role in defining the fusogenic properties necessary to mediate membrane movement between the glucose transporter vesicles, plasma membranes and micosomes
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
integral componant of FcepsilonRI mediated signal transduction mechanism
-
-
?
ATP + 1-phosphatidyl-1D-myo-inositol
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
neuronal calcium sensor-1 and the GTPase ARF1 are localized to the Golgi complex and regulate phosphatidylinositol 4-kinase IIIbeta. Spatial and temporal control of phosphatidylinositol 4-phosphate levels through activation of phosphatidylinositol 4-kinase IIIbeta is important for the recruitment of trafficking complexes to the trans-Golgi network and vesicular traffic from this organelle
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Mg2+
-
Mg2+ or Mn2+ required. Mg2+ at 15 mM gives maximal activity with 5 mM ATP
Mn2+
-
can only partially replace Mg2+. Activity at 2 mM or 5 mM is approximately 40% of the activity at 15 mM mM MgCl2
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
inosithin
-
a commercial preparation of ethanol-insoluble phospholipids from soybean; at high concentration
additional information
-
not affected by amyloid beta protein 20-29 and amyloid beta protein 31-35 alone, but both proteins are able to recover amyloid beta protein 25-35- or amyloid beta protein 2-42-induced inhibition at 0.1-5 nM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FcepsilonRI
-
ross-linking activates type II phosphatidylinositol 4-kinase
-
additional information
-
activity of the enzyme is dependent on SH groups
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.3 - 9
-
pH 5.3: about 80% of maximal activity, pH 8.6: about 60% of maximal activity
6.3 - 8.7
-
pH 6.3: about 50% of maximal activity, pH 8.7: about 80% of maximal activity
7.3 - 9
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pH 7.3: about 75% of maximal activity, pH 9.0: about 95% of maximal activity
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
the type IIalpha enzyme is concentrated both at the synapse and in the region of the Golgi complex in neuronal perikarya
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
no or minimal activity can be ascribed to mitochondria, lysosomes, Golgi membranes or endoplasmic reticulum
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-
PI4K230 is distributed evenly on membranes that are ultra structurally cisterns of the rough endoplasmic reticulum, outer membranes of mitochondria, multivesicular bodies and are in close vicinity of synaptic contacts
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isozyme PI4KIIalpha exists almost exclusively as a constitutively active integral membrane protein because of its palmitoylation
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
molecular chaperone Hsp90 is no binding partner of isozyme PI4KIIalpha, although also PI4KIIalpha contains an Hsp90 interaction site, which most likely resides in the N-terminal lobe of the catalytic domain, but PI4KIIalpha apparently does not require stabilization by Hsp90 binding because it associates strongly with membranes, even in the absence of palmitoylation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PI4KA_RAT
2096
0
236920
Swiss-Prot
Mitochondrion (Reliability: 3)
PI4KB_RAT
816
0
91655
Swiss-Prot
other Location (Reliability: 1)
P4K2A_RAT
478
0
54305
Swiss-Prot
other Location (Reliability: 1)
P4K2B_RAT
477
0
54464
Swiss-Prot
other Location (Reliability: 1)
A0A140TAJ5_RAT
2114
0
238130
TrEMBL
other Location (Reliability: 3)
A0A8I6GL94_RAT
480
0
54425
TrEMBL
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
lipoprotein
-
isozyme PI4KIIalpha exists almost exclusively as a constitutively active integral membrane protein because of its palmitoylation
phosphoprotein
-
the enzyme is an integral component of the early signal transduction machinery during T-cell activation by concanavalin A and is actively regulated by protein tyrosine phosphorylation-dephosphorylation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Colodzin, M.; Kennedy, E.P.
Biosynthesis of diphosphoinositide in brain
J. Biol. Chem.
240
3771-3780
1965
Rattus norvegicus
Kai, M.; White, G.L.; Hawthorne, J.N.
The phosphatidylinositol kinase of rat brain
Biochem. J.
101
328-337
1966
Rattus norvegicus
Cooper, P.H.; Hawthorne, J.N.
Phosphatidylinositol kinase and diphosphoinositide kinase of rat kidney cortex: properties and subcellular localization
Biochem. J.
160
97-105
1976
Rattus norvegicus
Collins, C.A.; Wells, W.W.
Identification of phosphatidylinositol kinase in rat liver lysosomal membranes
J. Biol. Chem.
258
2130-2134
1983
Rattus norvegicus
Lundberg, G.A.; Jergil, B.; Sundler, R.
Subcellular localization and enzymatic properties of rat liver phosphatidylinositol-4-phosphate kinase
Biochim. Biophys. Acta
846
379-387
1985
Rattus norvegicus
Del Vecchio, R.L.; Pilch, P.F.
Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles
J. Biol. Chem.
266
13278-13283
1991
Rattus norvegicus
Yamakawa, A.; Takenawa, T.
Purification and characterization of membrane-bound phosphatidylinositol kinase from rat brain
J. Biol. Chem.
263
17555-17560
1988
Rattus norvegicus
Heilmeyer, L.Jr.; Vereb, G.Jr.; Vereb, G.; Kakuk, A.; Szivak, I.
Mammalian phosphatidylinositol 4-kinases
IUBMB Life
55
59-65
2003
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus
Fernandis, A.Z.; Subrahmanyam, G.
Concanavalin A modulates tyrosine phosphorylation and activation of a type II phosphatidylinositol 4-kinase in rat splenic lymphocytes
Mol. Immunol.
35
935-941
1998
Rattus norvegicus
Zhao, X.; Varnai, P.; Tuymetova, G.; Balla, A.; Toth, Z.E.; Oker-Blom, C.; Roder, J.; Jeromin, A.; Balla, T.
Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase b stimulates lipid kinase activity and affects membrane trafficking in COS-7 cells
J. Biol. Chem.
276
40183-40189
2001
Bos taurus, Rattus norvegicus
Guo, J.; Wenk, M.R.; Pellegrini, L.; Onofri, F.; Benfenati, F.; De Camilli, P.
Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles
Proc. Natl. Acad. Sci. USA
100
3995-4000
2003
Rattus norvegicus
Verghese, M.; Fernandis, A.Z.; Subrahmanyam, G.
Purification and characterization of a type II phosphatidylinositol 4-kinase from rat spleen and comparison with a PtdIns 4-kinase from lymphocytes
Indian J. Biochem. Biophys.
36
1-9
1999
Rattus norvegicus
Haynes, L.P.; Thomas, G.M.; Burgoyne, R.D.
Interaction of neuronal calcium sensor-1 and ADP-ribosylation factor 1 allows bidirectional control of phosphatidylinositol 4-kinase beta and trans-Golgi network-plasma membrane traffic
J. Biol. Chem.
280
6047-6054
2005
Rattus norvegicus
Naveen, B.; Shankar, B.S.; Subrahmanyam, G.
FcepsilonRI cross-linking activates a type II phosphatidylinositol 4-kinase in RBL 2H3 cells
Mol. Immunol.
42
1541-1549
2005
Rattus norvegicus
Kakuk, A.; Friedlaender, E.; Vereb, G.; Kasa, A.; Balla, A.; Balla, T.; Heilmeyer, L.M.; Gergely, P.; Vereb, G.
Nucleolar localization of phosphatidylinositol 4-kinase PI4K230 in various mammalian cells
Cytometry A
69
1174-1183
2006
Homo sapiens, Rattus norvegicus
Ishihara, Y.; Furuno, T.; Nakanishi, M.
The role of phosphatidylinositol 4-kinase type IIalpha in degranulation of RBL-2H3 cells
Inflamm. Res.
55
465-468
2006
Rattus norvegicus
Wu, B.; Kitagawa, K.; Liu, B.; Zhang, N.Y.; Xiong, Z.M.; Inagaki, C.
Attenuation of amyloid beta (Abeta)-induced inhibition of phosphatidylinositol 4-kinase activity by Abeta fragments, Abeta20-29 and Abeta31-35
Neurosci. Lett.
396
148-152
2006
Rattus norvegicus
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