Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.1.40 - pyruvate kinase and Organism(s) Sus scrofa

for references in articles please use BRENDA:EC2.7.1.40
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
UTP, GTP, CTP, ITP and dATP can also act as donors. Also phosphorylates hydroxylamine and fluoride in the presence of CO2.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Sus scrofa
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Sus scrofa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pyruvate kinase, m2-pk, pyruvate kinase m2, l-pk, pyk, tum2-pk, pyruvate kinase type m2, liver pyruvate kinase, pyruvate kinase m2 isoform, m2-pyruvate kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CTHBP
-
-
-
-
cytosolic thyroid hormone binding protein
-
-
-
-
fluorokinase
-
-
-
-
kinase, fluoro- (phosphorylating)
-
-
-
-
kinase, pyruvate (phosphorylating)
-
-
-
-
L-PK
-
-
-
-
phosphoenol transphosphorylase
-
-
-
-
phosphoenolpyruvate kinase
-
-
-
-
PK-M1
-
-
pyruvate kinase muscle isozyme
-
-
-
-
pyruvate phosphotransferase
-
-
-
-
pyruvic kinase
-
-
-
-
R-type/L-type pyruvate kinase
-
-
-
-
red cell/liver pyruvate kinase
-
-
-
-
THBP1
-
-
-
-
VEG17
-
-
-
-
vegetative protein 17
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + pyruvate = ADP + phosphoenolpyruvate
show the reaction diagram
hyperbolic kinetics
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:pyruvate 2-O-phosphotransferase
UTP, GTP, CTP, ITP and dATP can also act as donors. Also phosphorylates hydroxylamine and fluoride in the presence of CO2.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-59-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphoenolpyruvate
ATP + pyruvate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
specificity overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + phosphoenolpyruvate
ATP + pyruvate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose 1,6-diphosphate
D-fructose diphosphate
-
activation
Triton X-100
-
50% increase of activity of the bound enzyme only
additional information
-
not activated by fructose 1,6-bisphosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 0.6
ADP
0.02 - 1.1
phosphoenolpyruvate
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
-
sperm homogenate
116.7
-
after 972fold purification
191
-
pH 7.4, 22°C
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
mitochondrial phosphoenolpyruvate kinase is restricted to the matrix
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A287AQJ5_PIG
1137
0
126286
TrEMBL
other Location (Reliability: 1)
Q29582_PIG
108
0
11923
TrEMBL
other Location (Reliability: 3)
A0A8D0YV78_PIG
657
0
71459
TrEMBL
other Location (Reliability: 2)
A0A8D1XH64_PIG
531
0
58033
TrEMBL
other Location (Reliability: 2)
A0A8D0IQ14_PIG
553
0
59524
TrEMBL
other Location (Reliability: 5)
F1SHL9_PIG
1163
0
129403
TrEMBL
other Location (Reliability: 1)
A0A8D0YSZ7_PIG
644
0
70210
TrEMBL
other Location (Reliability: 5)
A0A8D1M305_PIG
507
0
54839
TrEMBL
other Location (Reliability: 3)
A0A8D0TNG6_PIG
657
0
71440
TrEMBL
other Location (Reliability: 2)
A0A4X1TFN4_PIG
436
0
47444
TrEMBL
other Location (Reliability: 2)
A0A287BAZ7_PIG
561
0
60611
TrEMBL
Mitochondrion (Reliability: 3)
F1SHM0_PIG
736
0
80251
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8D0YWF0_PIG
576
0
62131
TrEMBL
other Location (Reliability: 2)
A0A286ZI36_PIG
657
0
71431
TrEMBL
other Location (Reliability: 2)
A0A8D0S108_PIG
570
0
62151
TrEMBL
Mitochondrion (Reliability: 3)
A0A8D0S274_PIG
613
0
67055
TrEMBL
Mitochondrion (Reliability: 5)
A0A8D1KF59_PIG
657
0
71416
TrEMBL
other Location (Reliability: 2)
A0A8D0Y3B4_PIG
657
0
71416
TrEMBL
other Location (Reliability: 2)
A0A4X1W1T3_PIG
574
0
61755
TrEMBL
other Location (Reliability: 2)
A0A4X1W0M5_PIG
562
0
61065
TrEMBL
Mitochondrion (Reliability: 3)
A0A8D1RA52_PIG
1095
0
121501
TrEMBL
other Location (Reliability: 1)
A0A8D1WGB3_PIG
657
0
71446
TrEMBL
other Location (Reliability: 2)
A0A287B8G0_PIG
1090
0
121307
TrEMBL
other Location (Reliability: 1)
A0A8D0S2N1_PIG
623
0
68286
TrEMBL
other Location (Reliability: 2)
A0A4X1VZC4_PIG
561
0
60611
TrEMBL
Mitochondrion (Reliability: 3)
A0A4X1TH79_PIG
1109
0
123399
TrEMBL
other Location (Reliability: 1)
A0A8D0XGM6_PIG
574
0
62008
TrEMBL
other Location (Reliability: 5)
A0A8D1JD21_PIG
606
0
66537
TrEMBL
Mitochondrion (Reliability: 5)
A0A480TIS7_PIG
579
0
63182
TrEMBL
Mitochondrion (Reliability: 5)
A0A8D0KC07_PIG
355
0
37983
TrEMBL
other Location (Reliability: 3)
A0A4X1TD68_PIG
1093
0
121535
TrEMBL
other Location (Reliability: 1)
A0A8D0XGL4_PIG
510
0
54937
TrEMBL
other Location (Reliability: 3)
A0A8D0Y5A1_PIG
656
0
71949
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8D1XHU8_PIG
531
0
57878
TrEMBL
other Location (Reliability: 2)
A0A4X1TD39_PIG
1117
0
124152
TrEMBL
other Location (Reliability: 1)
A0A8D2BZF2_PIG
577
0
63086
TrEMBL
other Location (Reliability: 2)
A0A8D1T8R0_PIG
656
0
71931
TrEMBL
Secretory Pathway (Reliability: 1)
A0A4X1THG9_PIG
624
0
68593
TrEMBL
Mitochondrion (Reliability: 4)
A0A480PP31_PIG
553
0
59524
TrEMBL
other Location (Reliability: 5)
A0A8D1JA09_PIG
1117
0
124307
TrEMBL
other Location (Reliability: 1)
A0A8D1R578_PIG
657
0
71431
TrEMBL
other Location (Reliability: 2)
A0A4X1TES0_PIG
982
0
108977
TrEMBL
other Location (Reliability: 3)
A0A8D0XGJ0_PIG
548
0
58983
TrEMBL
Mitochondrion (Reliability: 2)
A0A480JGH8_PIG
531
0
57878
TrEMBL
other Location (Reliability: 2)
A0A8D1T920_PIG
1137
0
126268
TrEMBL
other Location (Reliability: 1)
A0A286ZYV7_PIG
565
0
61163
TrEMBL
Mitochondrion (Reliability: 3)
Q9TTN8_PIG
41
0
4358
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D0YQW8_PIG
1137
0
126286
TrEMBL
other Location (Reliability: 1)
A0A287B785_PIG
1202
0
133624
TrEMBL
other Location (Reliability: 1)
A0A4X1TEM7_PIG
579
0
63182
TrEMBL
Mitochondrion (Reliability: 5)
A0A8D0HV34_PIG
203
0
21691
TrEMBL
other Location (Reliability: 1)
A0A8D1LNW9_PIG
1095
0
121483
TrEMBL
other Location (Reliability: 1)
A0A287A7R4_PIG
1156
0
128528
TrEMBL
other Location (Reliability: 1)
A0A8D1XMA7_PIG
657
0
71388
TrEMBL
other Location (Reliability: 2)
A0A287B7L9_PIG
1095
0
121501
TrEMBL
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000
-
sucrose density gradient centrifugation, M1 isozyme
237000
-
sucrose density gradient centrifugation
250000
265000
-
liver, gel filtration
59000
-
4 * 59000, SDS-PAGE
60000
62000
-
4 * 62000, liver enzyme, SDS-PAGE
63000
-
4 * 63000, SDS-PAGE
64500
-
SDS-PAGE, subunit
additional information
-
overview
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
-
structure overview
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme cannot be solubilized in detergents, but trypsin without loss of activity
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
cannot be solubilized in detergents without loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Fractogel EMD SO3- ion exchange chromatography and Fractogel EMD propyl (S) hydrophobic interaction chromatography
-
partially purified enzyme preparations from various organisms: overview
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kiffmeyer, W.R.; Farrar, W.W.
Purification and properties of pig heart pyruvate kinase
J. Protein Chem.
10
585-591
1991
Sus scrofa
Manually annotated by BRENDA team
Kobayashi, H.; Ozawa, K.; Yamada, S.
Physical and catalytic properties of pyruvate kinase from pig dental pulp
Arch. Oral Biol.
31
559-563
1986
Sus scrofa
Manually annotated by BRENDA team
Kayne, F.J.
Pyruvate kinase
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
8
353-382
1973
Azotobacter vinelandii, Komagataeibacter xylinus, Antarctic fish, Bacillus subtilis, [Brevibacterium] flavum, Saccharomyces cerevisiae, Coprinopsis lagopus, Oryctolagus cuniculus, Schistocerca gregaria, Escherichia coli, Euglena gracilis, Cobitidae, Amylomyces rouxii, Mus musculus, Oncorhynchus mykiss, Ostreidae, Paralithodes camtschaticus, Lithobates pipiens, Rattus norvegicus, Saccharomyces pastorianus, Sus scrofa, Halothiobacillus neapolitanus, Trypanosoma brucei
-
Manually annotated by BRENDA team
Farrar, G.; Farrar, W.W.
Purification and properties of the pyruvate kinase isozyme M1 from the pig brain
Int. J. Biochem. Cell Biol.
27
1145-1151
1995
Sus scrofa
Manually annotated by BRENDA team
Feiden, S.; Stypa, H.; Wolfrum, U.; Wegener, G.; Kamp, G.
A novel pyruvate kinase (PK-S) from boar spermatozoa is localized at the fibrous sheath and the acrosome
Reproduction
134
81-95
2007
Sus scrofa
Manually annotated by BRENDA team
Pizzuto, R.; Paventi, G.; Atlante, A.; Passarella, S.
Pyruvate kinase in pig liver mitochondria
Arch. Biochem. Biophys.
495
42-48
2010
Sus scrofa
Manually annotated by BRENDA team
Feiden, S.; Wolfrum, U.; Wegener, G.; Kamp, G.
Expression and compartmentalisation of the glycolytic enzymes GAPDH and pyruvate kinase in boar spermatogenesis
Reprod. Fertil. Dev.
20
713-723
2008
Sus scrofa
Manually annotated by BRENDA team