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Information on EC 2.7.1.32 - choline kinase and Organism(s) Caenorhabditis elegans
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2.7.1.32 choline kinaseIUBMB Comments
Ethanolamine and its methyl and ethyl derivatives can also act as acceptors.
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Word Map
- 2.7.1.32
- phosphatidylcholine
- phospholipid
- phosphocholine
- cytidylyltransferase
- cdp-choline
- phosphorylcholine
- phosphatidylethanolamine
-
ctp:phosphocholine
-
cholinephosphotransferase
-
kennedy
-
cholinephosphate
- glycerophosphocholine
- phosphatidate
-
choline-containing
- hemicholinium-3
- phosphoethanolamine
-
bispyridinium
-
3hcholine
-
2.7.7.15
-
ptdcho
- diagnostics
- medicine
- 1,2-diacylglycerol
- pcyt1a
- drug development
- cdp-ethanolamine
- biotechnology
-
2.7.8.2
-
phosphatidylcholine-specific
-
methyl-3hcholine
-
methyl-14ccholine
The taxonomic range for the selected organisms is: Caenorhabditis elegans
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
choline kinase, choline kinase alpha, chokalpha, choline kinase beta, choline/ethanolamine kinase, chk-alpha, chokalpha1, schok, ck-alpha, choline kinase-alpha, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CHETK-alpha
-
-
-
-
ChoK
-
-
-
-
choline phosphokinase
-
-
-
-
choline-ethanolamine kinase
-
-
-
-
choline/ethanolamine kinase
-
-
-
-
EtnK
-
-
-
-
kinase, choline (phosphorylating)
-
-
-
-
additional information
-
at least four genes encoding active choline kinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:choline phosphotransferase
Ethanolamine and its methyl and ethyl derivatives can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY
9026-67-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + ethanolamine
ADP + ethanolamine phosphate
-
poor substrate compared to choline
-
-
?
ATP + choline
ADP + O-phosphocholine
-
-
-
-
?
ATP + choline
ADP + O-phosphocholine
-
initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + choline
ADP + O-phosphocholine
-
initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CKB1_CAEEL
371
0
42786
Swiss-Prot
other Location (Reliability: 2)
CKB2_CAEEL
369
0
42264
Swiss-Prot
other Location (Reliability: 2)
CKB3_CAEEL
368
0
41928
Swiss-Prot
other Location (Reliability: 4)
CKA2_CAEEL
429
0
49240
Swiss-Prot
other Location (Reliability: 3)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40600
-
isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence
42000
48600
-
isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence
49000
-
isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence
51000
-
2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration
42000
-
isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
isoform CKB-2: x * 40600, SDS-PAGE, x * 42000, deduced from gene sequence
dimer
dimer
-
isoform CKA-2: x * 48600, SDS-PAGE, x * 49000, deduced from gene sequence
dimer
-
2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D255A
D255E
D255N
D301A
D301E
D301N
E125A
E125D
E125Q
E303A
E303D
E320A
H253A
N254A
-
similar initial activity like wild type enzyme, but time instability
N260A
N308D
N308Q
N354A
-
strongly reduced activity, only minor effect on Km for both substrates
N87A
R111A
R149A
S86A
W387A
E303A
-
strongly reduced activity, increased Km for both substrates, strongly increased Km for Mg2+
H253A
-
similar initial activity like wild type enzyme, but time instability
N260A
-
activity almost completely abolished, strongly increased Km for Mg2+
W387A
-
strongly reduced activity, only minor effect on Km for both substrates
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
native and mutant enzymes expressed as His-tag fusion protein in Sf9 cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gee, P.; Kent, C.
Multiple isoforms of choline kinase from Caenorhabditis elegans: cloning, expression, purification, and characterization
Biochim. Biophys. Acta
1648
33-42
2003
Caenorhabditis elegans
Peisach, D.; Gee, P.; Kent, C.; Xu, Z.
The crystal structure of choline kinase reveals a eukaryotic protein kinase fold
Structure
11
703-713
2003
Caenorhabditis elegans
Yuan, C.; Kent, C.
Identification of critical residues of ATP kinase A2 from Caenorhabditis elegans
J. Biol. Chem.
279
17801-17809
2004
Caenorhabditis elegans
Aoyama, C.; Liao, H.; Ishidate, K.
Structure and function of choline kinase isoforms in mammalian cells
Prog. Lipid Res.
43
266-281
2004
Saccharomyces cerevisiae, Caenorhabditis elegans, Homo sapiens, Mus musculus, Rattus norvegicus
Milanese, L.; Espinosa, A.; Campos, J.M.; Gallo, M.A.; Entrena, A.
Insight into the inhibition of human choline kinase: homology modeling and molecular dynamics simulations
ChemMedChem
1
1216-1228
2006
Caenorhabditis elegans, Homo sapiens
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