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Information on EC 2.7.1.22 - ribosylnicotinamide kinase and Organism(s) Homo sapiens
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2.7.1.22 ribosylnicotinamide kinaseSpecify your search results
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
nicotinamide riboside kinase, nmrk2, nrk-2, nicotinamide riboside kinase 2, ribosylnicotinamide kinase, nmrk1, nrk2b, nmr-k, nicotinamide riboside kinase 1, muscle-specific beta1 integrin binding protein, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kinase, ribosylnicotinamide (phosphorylating)
-
-
-
-
NRK1
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:N-ribosylnicotinamide 5'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9030-61-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide
ADP + beta-nicotinamide D-ribonucleotide
-
-
-
?
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide
GDP + beta-nicotinamide D-ribonucleotide
-
-
-
?
additional information
?
-
enzyme NRK2 highly prefers ATP, while isozyme NRK1 also uses GTP with similar activity
-
-
-
additional information
?
-
enzyme NRK2 highly prefers ATP, while isozyme NRK1 also uses GTP with similar activity
-
-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide
ADP + beta-nicotinamide D-ribonucleotide
-
-
-
?
GTP + 1-(beta-D-ribofuranosyl)-nicotinamide
GDP + beta-nicotinamide D-ribonucleotide
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.068
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with GTP
0.088
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with ATP
0.19
1-(beta-D-ribofuranosyl)-nicotinamide
with ATP, pH and temperature not specified in the publication
30
1-(beta-D-ribofuranosyl)-nicotinamide
with GTP, pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.34
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with GTP
0.6
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with ATP
0.75
1-(beta-D-ribofuranosyl)-nicotinamide
with ATP, pH and temperature not specified in the publication
1.7
1-(beta-D-ribofuranosyl)-nicotinamide
with GTP, pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.057
1-(beta-D-ribofuranosyl)-nicotinamide
with GTP, pH and temperature not specified in the publication
3.95
1-(beta-D-ribofuranosyl)-nicotinamide
with ATP, pH and temperature not specified in the publication
5
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with GTP
6.82
1-(beta-D-ribofuranosyl)-nicotinamide
pH and temperature not specified in the publication, with ATP
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
predominantly expresses in skeletal muscle with a trace amount expressed in healthy cardiac tissue
predominantly expresses in skeletal muscle with a trace amount expressed in healthy cardiac tissue
additional information
-
presence of quinolinate phosphoribosyltransferase and nicotinamide riboside kinase in all examined tissues/cell lines
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
NRK-2 plays a critical role in heart failure progression following ischemic injury. NRK-2 deficiency promotes post-MI scar expansion, rapid LV chamber dilatation, cardiac dysfunction and fibrosis possibly due to increased p38slphs activation
metabolism
the enzyme is involved in the NAD+ biosynthesis pathway. In the initial step of the pathway, NRK activity catalyses the phosphorylation of nicotinamide riboside (NR) to nicotinamide mononucleotide (NMN). Importance of different salvage pathways involved in metabolising the vitamin B3 class of NAD+ precursor molecules, with a particular focus on the nicotinamide riboside kinase pathway at both a tissue-specific and systemic level, regulation of the NRK enzymes, overview. Alternatively, NRK activity can phosphorylate nicotinic acid riboside (NaR) to nicotinic acid mononucleotide (NaMN), see for EC 2.7.1.173
physiological function
additional information
physiological function
NRK-2 plays a critical role in heart failure progression following ischemic injury. NRK-2 deficiency promotes post-MI scar expansion, rapid LV chamber dilatation, cardiac dysfunction and fibrosis possibly due to increased p38slphs activation
physiological function
NRK2 appears to play a redundant role in NAD+ biosynthesis along with NRK1, at least in unchallenged models, its highly regulated expression particularly in times of stress suggest it may have role beyond NAD+ metabolism
additional information
proposed NRK expression in disease and potential therapeutic interventions
additional information
proposed NRK expression in disease and potential therapeutic interventions
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NRK2_HUMAN
230
0
26046
Swiss-Prot
other Location (Reliability: 3)
NRK1_HUMAN
199
0
23193
Swiss-Prot
other Location (Reliability: 2)
PLAT2_HUMAN
162
1
17394
Swiss-Prot
other Location (Reliability: 3)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion method at 22°C, crystal structures of NRK1 in a binary complex with the reaction product nicotinamide mononucleotide at 1.5 A resolution and in a ternary complex with ADP and tiazofurin at 2.7 A resolution. The active site is located in a groove between the central parallel beta sheet core and the LID and NMP-binding domains
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Nmrk2 mRNA expression is substantially induced (over 80fold) in models of lethal cardiomyopathy
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
simple, fast and sensitive coupled fluorometric assay that enables simultaneous determination of nicotinamide phosphoribosyltransferase EC 2.4.2.12, quinolinate phosphoribosyltransferase EC 2.4.2.19, nicotinate phosphoribosyltransferase EC 6.3.4.21 and nicotinamide riboside kinase in whole-cell extracts and biological fluids yielding an overall picture of the tissue/cell-specific distribution of the activities of the various enzymes
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Khan, J.A.; Xiang, S.; Tong, L.
Crystal structure of human nicotinamide riboside kinase
Structure
15
1005-1013
2007
Homo sapiens (Q9NWW9), Homo sapiens
Zamporlini, F.; Ruggieri, S.; Mazzola, F.; Amici, A.; Orsomando, G.; Raffaelli, N.
Novel assay for simultaneous measurement of pyridine mononucleotides synthesizing activities allows dissection of the NAD+ biosynthetic machinery in mammalian cells
FEBS J.
281
5104-5119
2014
Homo sapiens
Ahmad, F.; Tomar, D.; Aryal A C, S.; Elmoselhi, A.B.; Thomas, M.; Elrod, J.W.; Tilley, D.G.; Force, T.
Nicotinamide riboside kinase-2 alleviates ischemia-induced heart failure through P38 signaling
Biochim. Biophys. Acta
1866
165609
2020
Mus musculus (Q9D7C9), Mus musculus, Homo sapiens (Q9NPI5), Homo sapiens, Mus musculus C57BL/6N (Q9D7C9)
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