Information on EC 2.7.1.21 - thymidine kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
2.7.1.21
-
RECOMMENDED NAME
GeneOntology No.
thymidine kinase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + thymidine = ADP + dTMP
show the reaction diagram
mechanism
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
the amino acid residues at positions 51, 83, and 185 are important for enzyme activity
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
molecular docking of substrates and inhibitors, active site structure modeling
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
active site structure, E98 is involved in catalysis
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
active site structure
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
active site structure
ATP + thymidine = ADP + dTMP
show the reaction diagram
active site structure
ATP + thymidine = ADP + dTMP
show the reaction diagram
active site structure, substrate binding structures
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
substrate binding and active site structure
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
substrate binding site structures, structural basis for substrate specificity and activity
Herpes simplex virus type 4
-
ATP + thymidine = ADP + dTMP
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pyrimidine deoxyribonucleosides salvage
-
-
pyrimidine metabolism
-
-
Pyrimidine metabolism
-
-
Drug metabolism - other enzymes
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:thymidine 5'-phosphotransferase
Deoxyuridine can also act as acceptor, and dGTP can act as a donor. The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 2.7.1.114 (AMP---thymidine kinase), EC 2.7.1.118 (ADP---thymidine kinase) and EC 2.7.4.9 (dTMP-kinase).
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2'-deoxythymidine kinase
-
-
-
-
2'-deoxythymidine kinase
-
-
ATP-thymidine 5'-phosphotransferase
-
-
-
-
beta-thymidine kinase-1
-
-
beta-thymidine kinase-2
-
-
calmodulin-binding protein
-
CaM-binding protein
-
dThd kinase-1
-
-
dThd kinase-2
-
-
EC 2.7.1.75
-
-
-
-
EHV4-TK
Herpes simplex virus type 4
-
-
HSV-1 TK
Herpes simplex virus
-
-
HSV1-TK
Herpes simplex virus
-
-
kinase, deoxythymidine (phosphorylating)
-
-
-
-
kinase, thymidine (phosphorylating)
-
-
-
-
TdR kinase
-
-
-
-
thymidine kinase
-
-
thymidine kinase 1
-
thymidine kinase 1
-
thymidine kinase 1
Q9S750 and Q9FN47
-
thymidine kinase 1
-
thymidine kinase 1
-
-
thymidine kinase 1
-
thymidine kinase 1
-
thymidine kinase 1
-
thymidine kinase 1
Herpes simplex virus
-
-
thymidine kinase 1
-
-
thymidine kinase 1
-
-
thymidine kinase 1
-
thymidine kinase 1
-
thymidine kinase 1
-
thymidine kinase 1
-
-
thymidine kinase 2
-
-
thymidine kinase 2
-
-
thymidine kinase 2, mitochondrial
-
thymidine kinase I
-
-
thymidine kinase I
-
-
-
thymidine kinase I
-
-
thymidine kinase I
-
-
thymidine kinase I
-
-
thymidine kinase I
Pyrobaculum aerophilum IM2
-
-
-
thymidine kinase-1
-
-
thymidine kinase-2
-
-
thymidylate kinase
Herpes simplex virus type 4
-
-
TK-1
-
-
TK1
Q9S750 and Q9FN47
-
TK1
Pyrobaculum aerophilum IM2
-
-
-
type 1 thymidine kinase
Herpes simplex virus
-
-
type II thymidine kinase 1
-
type II thymidine kinase 1
-
-
type II thymidine kinase 1
-
type II thymidine kinase 1
-
UL23
-
gene name
UL23
Human herpesvirus 1 L2
-
gene name
-
mitochondrial thymidine kinase
-
-
additional information
-
the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes
additional information
-
the enzyme belongs to the thymidine kinase 2, i.e. TK2, family of enzymes
additional information
the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes
additional information
-
the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes
CAS REGISTRY NUMBER
COMMENTARY
9002-06-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
duck
-
-
Manually annotated by BRENDA team
ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ecotype Columbia-0
SwissProt
Manually annotated by BRENDA team
subunits alpha and beta
Q9S750 and Q9FN47
UniProt
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
strain TH20p
-
-
Manually annotated by BRENDA team
Equid herpesvirus 4 TH20p
strain TH20p
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
-
-
Manually annotated by BRENDA team
Herpes simplex virus
type 1; type 2
-
-
Manually annotated by BRENDA team
Herpes simplex virus type 4
type 4
-
-
Manually annotated by BRENDA team
Herpes simplex virus type-1
-
-
-
Manually annotated by BRENDA team
enzyme contains a Val at position 106
-
-
Manually annotated by BRENDA team
i.e. HSV-1, acyclovir-sensitive strain KOS, genetic polymorphism
-
-
Manually annotated by BRENDA team
Human herpesvirus 1 L2
-
-
-
Manually annotated by BRENDA team
i.e. human Herpes virus 3, VZV
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
-
-
Manually annotated by BRENDA team
Murine gammaherpesvirus-68
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Pyrobaculum aerophilum IM2
strain IM2
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
blood stream and epimastigote form
-
-
Manually annotated by BRENDA team
Trypanosoma brucei TC221
-
-
-
Manually annotated by BRENDA team
of Amsacta moorei
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
-
-
Manually annotated by BRENDA team
the thymidine kinases from Gram-positive bacteria are more closely related to the eukaryotic thymidine kinase 1 enzymes than are thymidine kinases from Gram-negative bacteria
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
the thymidine kinase-deletion mutant WA79DELTATK shows more than 20fold increase in sensitivity towards acyclovir and is insensitive to ganciclovir
malfunction
TK1a/TK1b double mutants die at an early stage
malfunction
-
depletion of thymidine kinase 1 decreases the efficiency of DNA repair during recovery from DNA damage and generates more cell death
malfunction
-
isoform TK2-deficient animal show growth retardation and premature death
malfunction
Herpes simplex virus
-
a mutation in the thymidine kinase gene results in a shortened enzyme incapable of E-5-(2-bromovinyl)-2'-deoxyuridine phosphorylating
malfunction
Equid herpesvirus 4 TH20p
-
the thymidine kinase-deletion mutant WA79DELTATK shows more than 20fold increase in sensitivity towards acyclovir and is insensitive to ganciclovir
-
malfunction
Herpes simplex virus type-1
-
a mutation in the thymidine kinase gene results in a shortened enzyme incapable of E-5-(2-bromovinyl)-2'-deoxyuridine phosphorylating
-
physiological function
-
the virus-encoded thymidine kinase is an important determinant of the virus susceptibility to nucleoside analogues
physiological function
-
thymidine kinase 1 Ccontributes to the increase in dTTP pool during recovery from DNA damage and affects the efficiency of DNA repair during recovery for G2/M progression
physiological function
-
thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells
physiological function
Equid herpesvirus 4 TH20p
-
the virus-encoded thymidine kinase is an important determinant of the virus susceptibility to nucleoside analogues
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
drug activation by phosphorylation
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
about 115% of the activity with 2'-deoxythymidine
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + 1-(beta-D-arabinofuranosyl)thymine
ADP + 1-(beta-D-arabinofuranosyl)thymine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
show the reaction diagram
-
as active as with 2'-deoxythymidine
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine
ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5-phosphate
show the reaction diagram
-
12% activity compared to thymidine
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5-phosphate
show the reaction diagram
Human herpesvirus 1, Human herpesvirus 1 L2
-
-
-
?
ATP + 2',3'-dideoxydehydrothymidine
ADP + 2',3'-dideoxydehydrothymidine 5-phosphate
show the reaction diagram
Human herpesvirus 1, Human herpesvirus 1 L2
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
induction of conformational changes upon ligand binding, overview
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
induction of conformational changes upon ligand binding, overview
-
ir
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-exo-methanocarba-thymidine
ADP + 2'-exo-methanocarba-thymidine 5'-monophosphate
show the reaction diagram
Herpes simplex virus
-
MCT
-
-
ATP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
ADP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine 5'-phosphate
show the reaction diagram
12.3% activity compared to thymidine
-
?
ATP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
ADP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
2.4% activity compared to thymidine
-
?
ATP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine
ADP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
3.1% activity compared to thymidine
-
?
ATP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine
ADP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine 5'-phosphate
show the reaction diagram
7.1% activity compared to thymidine
-
?
ATP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
2.7% activity compared to thymidine
-
?
ATP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
6.5% activity compared to thymidine
-
?
ATP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
ADP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine 5'-phosphate
show the reaction diagram
13.7% activity compared to thymidine
-
?
ATP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
ADP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
2.6% activity compared to thymidine
-
?
ATP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
11.6% activity compared to thymidine
-
?
ATP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
4.8% activity compared to thymidine
-
?
ATP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
2.9% activity compared to thymidine
-
?
ATP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
8.7% activity compared to thymidine
-
?
ATP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
2.1% activity compared to thymidine
-
?
ATP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
5.5% activity compared to thymidine
-
?
ATP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
1.9% activity compared to thymidine
-
?
ATP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
7.2% activity compared to thymidine
-
?
ATP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
2.2% activity compared to thymidine
-
?
ATP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
show the reaction diagram
4.7% activity compared to thymidine
-
?
ATP + 3'-azido-2',3'-deoxythymidine
ADP + 3'-azido-2',3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 3'-azido-2',3'-dideoxythymidine
ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
show the reaction diagram
-
about 165% of the activity with 2'-deoxythymidine
-
?
ATP + 3'-azido-2',3'-dideoxythymidine
ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
show the reaction diagram
-
about 5% of the activity with 2'-deoxythymidine
-
?
ATP + 3'-azido-2',3'-dideoxythymidine
ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
show the reaction diagram
-
60% activity compared to thymidine
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
show the reaction diagram
27.8% activity compared to thymidine
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
show the reaction diagram
-
47.3% activity compared to thymidine
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5-phosphate
show the reaction diagram
-
-
?
ATP + 3'-azidothymidine
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + 3'-deoxy-2',3'-didehydro-thymidine
ADP + 3'-deoxy-2',3'-didehydro-thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 3'-deoxy-2',3'-didehydrothymidine
ADP + 3'-deoxy-2',3'-didehydrothymidine 5-phosphate
show the reaction diagram
Human herpesvirus 1, Human herpesvirus 1 L2
-
-
-
?
ATP + 3'-deoxythymidine
ADP + 3'-deoxythymidine 5-phosphate
show the reaction diagram
Human herpesvirus 1, Human herpesvirus 1 L2
-
-
-
?
ATP + 3'-fluoro-3'-deoxythymidine
ADP + 3'-fluoro-3'-deoxythymidine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + 3-(benzylcarbamimidoyl)thymidine
ADP + 3-(benzylcarbamimidoyl)thymidine 5-phosphate
show the reaction diagram
-
14.9% activity compared to thymidine
-
?
ATP + 3-(butylcarbamimidoyl)thymidine
ADP + 3-(butylcarbamimidoyl)thymidine 5-phosphate
show the reaction diagram
-
1.2% activity compared to thymidine
-
?
ATP + 3-(phenylcarbamimidoyl)thymidine
ADP + 3-(phenylcarbamimidoyl)thymidine 5-phosphate
show the reaction diagram
-
1.9% activity compared to thymidine
-
?
ATP + 3-(tetrazol-5-ylmethyl)thymidine
ADP + 3-(tetrazol-5-ylmethyl)thymidine 5-phosphate
show the reaction diagram
-
39.5% activity compared to thymidine
-
?
ATP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine 5'-phosphate
show the reaction diagram
-
63.7% activity compared to thymidine
-
?
ATP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine 5'-phosphate
show the reaction diagram
-
42.5% activity compared to thymidine
-
?
ATP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine 5'-phosphate
show the reaction diagram
-
52.4% activity compared to thymidine
-
?
ATP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine 5'-phosphate
show the reaction diagram
-
55.6% activity compared to thymidine
-
?
ATP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine 5'-phosphate
show the reaction diagram
-
58.2% activity compared to thymidine
-
?
ATP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine 5'-phosphate
show the reaction diagram
-
42.9% activity compared to thymidine
-
?
ATP + 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine 5-phosphate
show the reaction diagram
-
55.7% activity compared to thymidine
-
?
ATP + 3-[1(2)-propyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-propyltetrazol-5-yl]thymidine 5-phosphate
show the reaction diagram
-
57.6% activity compared to thymidine
-
?
ATP + 3-[2-(chloro-1,7-carboranyl)ethylcarbamimidoyl]thymidine
ADP + 3-[2-(chloro-1,7-carboranyl)ethylcarbamimidoyl]thymidine 5-phosphate
show the reaction diagram
-
16.3% activity compared to thymidine
-
?
ATP + 3-[2-imino-2-(benzylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(benzylamino)ethyl]thymidine 5-phosphate
show the reaction diagram
-
16.8% activity compared to thymidine
-
?
ATP + 3-[2-imino-2-(butylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(butylamino)ethyl]thymidine 5-phosphate
show the reaction diagram
-
2.3% activity compared to thymidine
-
?
ATP + 3-[2-imino-2-(phenylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(phenylamino)ethyl]thymidine 5-phosphate
show the reaction diagram
-
11.3% activity compared to thymidine
-
?
ATP + 3-[2-imino-2-(propylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(propylamino)ethyl]thymidine 5-phosphate
show the reaction diagram
-
1.9% activity compared to thymidine
-
?
ATP + 3-[2-imino-2-[2-(chloro-1,7-carboranyl)ethylamino]ethyl]thymidine
ADP + 3-[2-imino-2-[2-(chloro-1,7-carboranyl)ethylamino]ethyl]thymidine 5-phosphate
show the reaction diagram
-
3.0% activity compared to thymidine
-
?
ATP + 3-[2-imino-2-[2-(chloro-1,7-carboranyl)propylamino]ethyl]thymidine
ADP + 3-[2-imino-2-[2-(chloro-1,7-carboranyl)propylamino]ethyl]thymidine 5-phosphate
show the reaction diagram
-
5.6% activity compared to thymidine
-
?
ATP + 3-[3-(chloro-1,7-carboranyl)propylcarbamimidoyl]thymidine
ADP + 3-[3-(chloro-1,7-carboranyl)propylcarbamimidoyl]thymidine 5-phosphate
show the reaction diagram
-
15.5% activity compared to thymidine
-
?
ATP + 3-[4-(chloro-1,7-carboranyl)butylcarbamimidoyl]thymidine
ADP + 3-[4-(chloro-1,7-carboranyl)butylcarbamimidoyl]thymidine 5-phosphate
show the reaction diagram
-
27.3% activity compared to thymidine
-
?
ATP + 5-azido-3'-azido-2',3'-dideoxyuridine
ADP + 5-azido-3'-azido-2',3'-dideoxyuridine 5'-monophosphate
show the reaction diagram
Herpes simplex virus
-
-
photoaffinity analog of 3'-azidothymidine
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-bromodeoxyuridine
ADP + 5-bromodeoxyuridine 5-phosphate
show the reaction diagram
-
84% activity compared to thymidine
-
?
ATP + 5-chloro-2'-deoxyuridine
ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-chloro-2'-deoxyuridine
ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-chloro-2'-deoxyuridine
ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-chlorodeoxyuridine
ADP + 5-chlorodeoxyuridine 5-phosphate
show the reaction diagram
-
110% activity compared to thymidine
-
?
ATP + 5-diaza-2'-deoxyuridine
ADP + 5-diaza-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5-phosphate
show the reaction diagram
-
good substrate, 120% activity compared to thymidine
-
?
ATP + 5-fluorodeoxyuridine
ADP + 5-fluorodeoxyuridine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
?
show the reaction diagram
5-iodo-2'-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate
-
-
?
ATP + 5-iododeoxyuridine
ADP + 5-iododeoxyuridine 5-phosphate
show the reaction diagram
-
89% activity compared to thymidine
-
?
ATP + 5-mercapto-2'-deoxyuridine
ADP + 5-mercapto-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-trifluoromethyl-2'-deoxyuridine
ADP + 5-trifluoromethyl-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 5-trifluoromethyl-2-deoxyuridine
?
show the reaction diagram
5-trifluoromethyl-2-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate
-
-
?
ATP + 6-aza-2'-deoxythymidine
ADP + 6-aza-2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine
ADP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + 9-[(hydroxyethyl)methyl]guanine
ADP + 9-[(hydroxyethyl)methyl]guanine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + aciclovir
ADP + aciclovir 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + aciclovir monophosphate
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + acyclovir
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + acyclovir
?
show the reaction diagram
Herpes simplex virus
-
poor substrate
-
-
?
ATP + acyclovir
ADP + acyclovir phosphate
show the reaction diagram
-
drug activation by phosphorylation, drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug
-
?
ATP + acyclovir
ADP + acyclovir 5-phosphate
show the reaction diagram
-
-
-
?
ATP + arabinosyl cytosine
?
show the reaction diagram
-
-
-
-
?
ATP + azidothymidine
?
show the reaction diagram
-
-
-
?
ATP + azidothymidine monophosphate
?
show the reaction diagram
-
-
-
?
ATP + beta-5-ethyl-2'-deoxyuridine
?
show the reaction diagram
beta-5-ethyl-2'-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate
-
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + beta-L-thymidine
ADP + beta-L-thymidine 5-phosphate
show the reaction diagram
-
less than 5% activity compared to thymidine
-
?
ATP + cytosine arabinoside 5'-triphosphate
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
Trypanosoma brucei, Trypanosoma brucei TC221
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
7% activity compared to thymidine
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
substrate for mutant enzymes M42I and L134A
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
Trypanosoma brucei TC221
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
about 20% activity compared to thymidine
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
substrate for mutant enzymes S182T and S182A
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
Trypanosoma brucei TC221
-
about 20% activity compared to thymidine
-
?
ATP + deoxyinosine
ADP + dIMP
show the reaction diagram
Trypanosoma brucei, Trypanosoma brucei TC221
-
about 30% activity compared to thymidine
-
?
ATP + deoxyuridine
ADP + dUMP
show the reaction diagram
-
-
?
ATP + deoxyuridine
ADP + dUMP
show the reaction diagram
-
69% activity compared to thymidine
-
?
ATP + deoxyuridine
ADP + dUMP
show the reaction diagram
-
about 185% activity compared to thymidine
-
?
ATP + dideoxyinosine
ADP + dideoxyinosine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine 5-phosphate
show the reaction diagram
-
-
-
?
ATP + ganciclovir
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + ganciclovir
ADP + ganciclovir phosphate
show the reaction diagram
-
-
-
?
ATP + ganciclovir monophosphate
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + guanosine
ADP + GMP
show the reaction diagram
-
1% activity compared to thymidine
-
?
ATP + iododideoxyuridine
ADP + iododideoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + lobucavir
?
show the reaction diagram
Herpes simplex virus
-
-
-
-
?
ATP + methylthymidine
ADP + methylthymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
-
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Murine gammaherpesvirus-68
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Q9S750 and Q9FN47
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
best substrate
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
100% activity
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
100% activity
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
100% activity
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
100% activity
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by GTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dGTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by ATP-arabinoside, ATP can be substituted by UTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by UTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by UTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by UTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dCTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dCTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dCTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
ATP can be substituted by dCTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dCTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
requirement for high levels of ATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
requirement for high levels of ATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Herpes simplex virus
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by dATP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by CTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by CTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by CTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by CTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by CTP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by ITP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
ATP can be substituted by ITP
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
best substrate combination, 100% activity
-
?
ATP + thymidine
ADP + dTMP
show the reaction diagram
Human herpesvirus 1 L2
-
-
-
?
ATP + thymidine
?
show the reaction diagram
-
-
-
-
?
ATP + thymidine
?
show the reaction diagram
-
-
-
-
?
ATP + thymidine
?
show the reaction diagram
-
involved in DNA replication
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
ir
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
Herpes simplex virus type 4
-
-
-
r
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
enzyme contains the putative thymidine-binding motif FQRK
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
ATP acts as specific phosphate donor
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
Equid herpesvirus 4 TH20p
-
-
-
?
ATP + thymidine 5'-phosphate
ADP + thymidine diphosphate
show the reaction diagram
Herpes simplex virus type 4
-
-
-
r
ATP + trifluoromethylthymidine
ADP + trifluoromethylthymidine 5-phosphate
show the reaction diagram
-
good substrate, 130% activity compared to thymidine
-
-
ATP + uridine
ADP + UMP
show the reaction diagram
-
-
-
?
ATP + [methyl-3H]thymidine
ADP + [methyl-3H]thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
CTP + thymidine
CDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
-
CTP + thymidine
CDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
-
CTP + thymidine
CDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
CTP + thymidine
CDP + dTMP
show the reaction diagram
-
-
-
?
dGTP + thymidine
dGDP + dTMP
show the reaction diagram
-
99% activity compared to ATP
-
?
GTP + thymidine
GDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
-
GTP + thymidine
GDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
-
GTP + thymidine
GDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
GTP + thymidine
GDP + dTMP
show the reaction diagram
-
-
-
?
TTP + thymidine
TDP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
UTP + thymidine
UDP + dTMP
show the reaction diagram
-
-
-
?
UTP + thymidine
UDP + dTMP
show the reaction diagram
-
17% activity compared to ATP
-
?
GTP + thymidine
GDP + dTMP
show the reaction diagram
-
42% activity compared to ATP
-
?
additional information
?
-
overview: nucleoside analogs
-
-
-
additional information
?
-
-
5-halogenated cytidines, bromouridine, iodouridine, uridine, 5-fluorodeoxycytidine, purine ribonucleosides
-
-
-
additional information
?
-
-
overview: thymidine analogs
-
-
-
additional information
?
-
-
comparison rat liver, Trypanosoma brucei
-
-
-
additional information
?
-
-
overview thymine nucleosides, 2'-deoxyribonucleosides, arabinosides, ribonucleosides
-
-
-
additional information
?
-
-
no phosphorylation of deoxyuridine, 5-halogenated deoxyuridines
-
-
-
additional information
?
-
-
no substrates: ganciclovir, acyclovir, 2'-deoxycytidine
-
-
-
additional information
?
-
-
overview: phosphoryl donors
-
-
-
additional information
?
-
-
the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs
-
-
-
additional information
?
-
-
the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model
-
-
-
additional information
?
-
-
the viral enzyme shows a broader substrate specificity than the human host cell enzyme
-
-
-
additional information
?
-
-
ligand-induced conformational changes in dimeric and tetrameric forms of the enzyme alter the catalytic activity
-
-
-
additional information
?
-
-
modeling of ganciclovir binding into the enzyme crystal structure, overview
-
-
-
additional information
?
-
narrow substrate specificity
-
-
-
additional information
?
-
-
substrate specificity of wild-type and mutant enzymes, overview
-
-
-
additional information
?
-
-
Val106 has effects on structure-function relation of the enzyme
-
-
-
additional information
?
-
Herpes simplex virus type 4
-
wide substrate specificity phosphorylating pyrimidine nucleosides, nucleoside monophophates, and several nucleoside analogues
-
-
-
additional information
?
-
-
the type II thymidine kinase ecoded by cowpox virus exhibits a more limited substrate specificity than the type I thymidine kinase encoded by HSV-1
-
-
-
additional information
?
-
beta-L-thymidine is poorly phosphorylated by the enzyme
-
-
-
additional information
?
-
does not use UTP, GTP, and TTP as cosubstrates
-
-
-
additional information
?
-
-
CTP, diphosphate, uridine, adenosine, cytosine, deoxyguanosine, deoxyguanosine, dTMP, thymine 1-beta-D-arabinofuranoside, 1-beta-D-arabinofuranosylcytosine, acyclovir, and 5-iodo-2'-deoxycytidine are no substrates
-
-
-
additional information
?
-
-
domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine
-
-
-
additional information
?
-
-
no activity with 3-(propylcarbamimidoyl)thymidine and 3-(tetrazol-5-yl)thymidine
-
-
-
additional information
?
-
-
the wild type enzyme shows no activity with deoxycytidine and deoxyadenosine
-
-
-
additional information
?
-
Trypanosoma brucei TC221
-
domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
drug activation by phosphorylation
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
P04183
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
P75070
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
O57203
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
ir
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + acyclovir
ADP + acyclovir phosphate
show the reaction diagram
-
drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug
-
?
ATP + thymidine
?
show the reaction diagram
-
-
-
-
?
ATP + thymidine
?
show the reaction diagram
-
-
-
-
?
ATP + thymidine
?
show the reaction diagram
-
involved in DNA replication
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
Q27564
-
-
ir
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
Herpes simplex virus type 4
-
-
-
r
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
Equid herpesvirus 4, Equid herpesvirus 4 TH20p
-
-
-
?
ATP + thymidine 5'-phosphate
ADP + thymidine diphosphate
show the reaction diagram
Herpes simplex virus type 4
-
-
-
r
additional information
?
-
-
the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs
-
-
-
additional information
?
-
-
the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model
-
-
-
additional information
?
-
-
the viral enzyme shows a broader substrate specificity than the human host cell enzyme
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
-
binding of ATP induces enzyme tetramerization
ATP
-
P-loop binding site
ATP
-
preferred triphosphate cosubstrate, 3 essential residues T298, K297, and G294 in the conserved ATP-binding region, enzyme binding kinetics
ATP
-
binding of ATP induces reversible transition from a dimer with low catalytic activiy to a tetramer with high catalytic activity
ATP
Herpes simplex virus type 4
-
-
CTP
-
less active than ATP
dATP
-
activation
dGTP
-
activation
GDP
-
activation
GTP
-
less active tahn ATP
TTP
-
low activity with wild-type and mutant enzymes
MgATP2-
-
-
additional information
-
activity with NTP cofactors in descending order ATP, GTP, CTP, TTP
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
can partially substitute for Mg2+
Ca2+
-
can partially substitute for Mg2+
Ca2+
-
can partially substitute for Mg2+
Ca2+
-
calmodulin binding binding is dependent on Ca2+
Co2+
-
can partially substitute for Mg2+
Co2+
-
can partially substitute for Mg2+
Co2+
-
can partially substitute for Mg2+
Cu2+
-
can partially substitute for Mg2+
Fe2+
-
can partially substitute for Mg2+
Mg2+
-
2.9 mM, required
Mg2+
-
Km: 0.8 mM
Mg2+
-
7.5-10 mM, required
Mg2+
-
-
Mg2+
-
required, most efficient metal ion, can be substituted by Mn2+ or Zn2+
Mg2+
Herpes simplex virus
-
required
Mg2+
MgCl2 is required for activity, reaching its optimal effect at 1.2 mM when ATP is present at 1 mM
Mn2+
-
can partially substitute for Mg2+
Mn2+
-
can partially substitute for Mg2+
Mn2+
-
can partially substitute for Mg2+
Mn2+
-
can partially substitute for Mg2+
Mn2+
-
can partially substitute for Mg2+
Ni2+
-
can partially substitute for Mg2+
Zn2+
-
can partially substitute for Mg2+
Zn2+
-
liver
Zn2+
-
can partially substitute for Mg2+
Zn2+
-
Cys residues C153, C156, C185, and C188 are involved in binding
Zn2+
-
enzyme contains a zinc finger binding motif
Zn2+
enzyme contains a zinc finger binding motif
Zn2+
enzyme contains a zinc finger binding motif
Zn2+
-
at a molar ratio of 0.56, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop
Zn2+
-
at a molar ratio of 0.51, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop
Mn2+
MnCl2 can replace MgCl2 in the assay, with optimal activity at 200 mM when ATP is present at 1 mM
additional information
-
inactive with Ba2+ and Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl 3,3-dimethylbutanoate
-
IC50: 0.028 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl 3,3-dimethylbutanoate
-
IC50: above 0.5 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl benzoate
-
IC50: 0.03 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl benzoate
-
IC50: above 0.5 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl diphenylacetate
-
IC50: 0.0046 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl diphenylacetate
-
IC50: 0.281 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl dodecanoate
-
IC50: 0.019 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl dodecanoate
-
IC50: above 0.5 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl heptanoate
-
IC50: 0.011 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl heptanoate
-
IC50: 0.42 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl pivalate
-
IC50: 0.04 mM
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl pivalate
-
IC50: above 0.5 mM
(4E)-N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
-
IC50: 0.265 mM
(4E)-N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
-
IC50: above 0.5 mM
(4E)-N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
-
IC50: 0.371 mM
(4E)-N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
-
IC50: above 0.5 mM
(E)-5-(2-bromovinyl)-1-beta-D-(arabinofuranosyl)uracil
-
-
1-(3'-deoxy-5'-O-triphenylmethyl-beta-D-thymidin-3'-yl)-3-(4-chloro-3-(trifluoromethyl)-phenyl)-thiourea
-
-
-
1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
-
-
1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
-
-
1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
-
-
1-beta-D-(arabinofuranosyl)thymine
-
-
1-[(2Z)-4-(4-benzylpiperazin-1-yl)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.18 mM
1-[(2Z)-4-(benzylamino)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.057 mM
1-[(2Z)-4-(benzyloxy)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.022 mM
1-[(2Z)-4-(benzyloxy)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: above 0.5 mM
1-[(2Z)-4-(dibenzylamino)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0035 mM; IC50: above 0.5 mM
1-[(2Z)-4-(dibenzylamino)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0035 mM
1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-5,6-dihydrothymine
-
IC50: 0.01 mM
1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-5,6-dihydrothymine
-
IC50: above 0.5 mM
1-[(2Z)-4-hydroxybut-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.173 mM
1-[(2Z)-4-hydroxybut-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.224 mM
1-[(Z)-4-(triphenylmethoxy)-2-butenyl]thymine
-
-
-
1-[2,3-dihydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.017 mM
1-[2,3-dihydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.013 mM
1-[3-hydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0036 mM
1-[3-hydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0012 mM
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea
-
-
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea
-
-
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
-
-
1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea
-
-
2'-deoxy-3'-aminothymidine
-
5 mM
2'-exo-methanocarba-thymidine
Herpes simplex virus
-
-
2-(4-chlorophenyl)-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
-
IC50: 0.023 mM
2-(4-chlorophenyl)-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
-
IC50: 0.198 mM
2-biphenyl-4-yl-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
-
IC50: 0.0041 mM
2-biphenyl-4-yl-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
-
IC50: 0.119 mM
2-mercaptoethanol
-
5 mM
3'-(1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
-
-
-
3'-(4-(2-phenyl-ethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
-
-
-
3'-(4-(2-phenyl-ethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
-
-
3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine
-
-
-
3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine
-
-
3'-(4-(3-chloro-propyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
-
3'-(4-(3-chloro-propyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
-
-
-
3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
-
-
3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
-
-
-
3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
-
3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
3'-(4-butyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
-
-
-
3'-(4-chlorobenzylamino)-3'-deoxy-beta-D-thymidine
-
-
-
3'-(4-chlorophenyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridine
-
-
-
3'-(4-chlorophenyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
-
-
-
3'-(4-cyclopentylmethyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
-
-
-
3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
-
3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
-
3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
-
3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
-
3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
-
-
3'-(5-(4-chlorophenyl)-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
-
-
-
3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine
-
-
3'-([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)-2',3'-dideoxy-3,4-dihydrothymidine
-
-
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
-
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
-
3'-azido-3'-deoxythymidine
-
-
3'-azido-3'-deoxythymidine
-
3'-benzylamino-3'-deoxy-beta-D-thymidine
-
-
-
3'-deoxy-3'-(4-(3,4-dichlorophenyl)-1,2,3-triazol-1-yl)-beta-D-thymidine
-
-
-
3'-deoxy-3'-(4-phenyl-1,2,3-triazol-1-yl)-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridine
-
-
-
3'-deoxy-3'-(4-phenyl-1,2,3-triazol-1-yl)-beta-D-thymidine
-
-
-
3'-deoxy-3'-(4-pyridin-2-yl-1,2,3-triazol-1-yl)-beta-D-thymidine
-
-
-
3'-deoxy-3'-(5-phenyl-1,2,3-triazol-1-yl)-beta-D-thymidine
-
-
-
3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine
-
-
3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine
-
-
3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine
-
-
3'-deoxy-3'-fluorothymidine
-
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
-
3'-ethyl-5-methyl-2'-deoxyuridine
-
3'-fluorothymidine
-
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
-
3'-O-ethyl-2'-deoxyuridine
-
-
3'-O-methyl-2'-deoxyuridine
-
-
3'-spiro-(4''-amino-1'',2''-oxathiole-2'',2''-dioxide)-5-methyluridine
-
inhibits phosphorylation of 2'-deoxythymidine, IC50: 0.0046 mM
3'-[(3-trifluoromethyl-4-chloro)-benzylamino]-3'-deoxy-beta-D-thymidine
-
-
-
3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine
-
-
3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine
-
-
3-N-methyl-5-iodo-2'-deoxyuridine
-
-
3-hexanoylamino-3'-deoxythymidine
-
very potent inhibitor of thymidine kinase 2
-
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylbutanamide
-
IC50: 0.44 mM
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylbutanamide
-
IC50: 0.175 mM
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-[2-oxo-2-(tritylamino)ethyl]butanamide
-
IC50: 0.194 mM
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-[2-oxo-2-(tritylamino)ethyl]butanamide
-
IC50: 0.191 mM
5'-aminothymidine
-
-
5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine
-
-
5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine
-
-
5'-O-(4,4'-dimethoxytrityl)thymidine
-
IC50: 0.468 mM
5-(3'-amino-3'-deoxy-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridin-3'Nyl)-1-(4-chloro-3-trifluoromethylphenyl)-tetrazole
-
-
-
5-(3'-amino-3'-deoxy-beta-D-thymidin-3'N-yl)-1-(4-chloro-3-trifluoromethylphenyl)-tetrazole
-
-
-
5-(3'-amino-3'-deoxy-beta-D-thymidin-3'N-yl)-1-benzyl-tetrazole
-
-
-
5-(E)-(2-bromovinyl)-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
-
IC50: 0.0013 mM; IC50: 0.1 mM
5-(E)-(2-bromovinyl)-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
-
IC50: above 0.5 mM
5-(E)-(2-bromovinyl)uridine
-
inhibits phosphorylation of 2'-deoxythymidine
5-(E)-(2-bromovinyl)uridine
-
-
5-aminobenzyl-(3'-deoxy-beta-D-thymidin-3'N-yl)-tetrazole
-
-
-
5-azido-2'-deoxyuridine
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM
5-azido-2'-deoxyuridine-5'-monophosphate
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant at 0.135 mM
5-bromodeoxyuridine
-
competitive to thymidine
5-bromodeoxyuridine
-
competitive to thymidine
5-bromodeoxyuridine
-
-
5-bromodeoxyuridine
-
-
5-bromovinyldeoxyuridine
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type enzyme at 0.0015 mM, 50% photoincorporation inhibition of C336Y mutant above 0.025 mM
5-Chlorodeoxyuridine
-
competitive to thymidine
5-ethyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
-
IC50: 0.02 mM
5-ethyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
-
IC50: above 0.5 mM
5-fluorodeoxyuridine
-
competitive to thymidine
5-fluorodeoxyuridine
-
competitive to thymidine
5-fluorodeoxyuridine
-
-
5-iodo-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
-
IC50: 0.0046 mM
5-iodo-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
-
IC50: 0.048 mM
5-iodo-2'-deoxyuridine
potent inhibitor
5-iododeoxyuridine
-
competitive to thymidine
5-iododeoxyuridine
-
competitive to thymidine
5-methyl-1-[(2E)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.025 mM
5-methyl-1-[(2E)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.003 mM
5-methyl-1-[(2Z)-4-(4-methylpiperazin-1-yl)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.491 mM
5-methyl-1-[(2Z)-4-(tritylamino)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0023 mM; IC50: above 0.5 mM
5-methyl-1-[(2Z)-4-(tritylamino)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0023 mM
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-cytosine
-
IC50: 0.484 mM
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-cytosine
-
IC50: above 0.5 mM
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0015 mM; IC50: above 0.1 mM
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.045 mM
5-methyl-1-[4-(trityloxy)but-2-yn-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.404 mM
5-methyl-1-[4-(trityloxy)but-2-yn-1-yl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0031 mM
5-methyl-1-[4-(trityloxy)butyl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.0033 mM
5-methyl-1-[4-(trityloxy)butyl]pyrimidine-2,4(1H,3H)-dione
-
IC50: 0.01 mM
5-propyl-2'-deoxyuridine
-
5-trifluoromethyl-2-deoxyuridine
potent inhibitor
5-Trifluoromethyldeoxyuridine
-
competitive to thymidine
5-[(E)-2-bromoethenyl]-2'-O-decanoyluridine
-
competitive with respect to 2'-deoxythymidine, IC50: 0.0012 mm
5-[(E)-2-bromoethenyl]-2'-O-octanoyluridine
-
IC50: 0.006 mM
5-[(E)-2-bromoethenyl]-2'-O-octanoyluridine
-
-
5-[amino-(4-chloro-3-trifluoromethylphenyl)]-1-(3'-deoxy-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridin-3'N-yl)-tetrazole
-
-
-
5-[amino-(4-chloro-3-trifluoromethylphenyl)]-1-(3'-deoxy-beta-D-thymidin-3'N-yl)-tetrazole
-
-
-
6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylhexanamide
-
IC50: 0.019 mM
6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylhexanamide
-
IC50: 0.0034 mM
6-methyluridine
-
9-[(2Z)-4-(trityloxy)but-2-en-1-yl]-guanine
-
IC50: above 0.5 mM
aciclovir
Herpes simplex virus
-
competitive inhibitor
acyclovir
Herpes simplex virus
-
syn. 9-[(2-hydroxyethoxy)methyl]guanine
acyclovir
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type enzyme at 0.05 mM, 50% photoincorporation inhibition of C336Y mutant above 0.3 mM
acyclovir H-phosphonate
-
-
-
adenine
-
-
ADP
-
competitive to ATP
ADP
-
competitive to ATP
alpha-D-5-ethyl-2'-deoxyuridine
-
alpha-L-thymidine
-
ammoniumsulfate
Herpes simplex virus
-
50% inhibition at 200 mM
ATP
-
non-competitive
ATP
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type enzyme at 0.15 mM, 50% photoincorporation inhibition of C336Y mutant above 1 mM
beta-D-5-ethyl-2'-deoxyuridine
potent inhibitor
beta-L-thymidine
potent inhibitor
bromo-dUTP
-
-
Caffeine
-
competitive to ATP
Caffeine
-
competitive to ATP, 75% inhibition at 20 mM
CH3CO2-
-
-
dATP
-
strong feedback inhibition, tight binding at the active site in stoichiometric amount
dCTP
-
mitochondrial enzyme
dCTP
-
30-45% inhibition of mitochondrial enzyme at 0.6 or 1 mM, no inhibition of cytosolic enzyme
dCTP
-
80% inhibition of adult enzyme at 1 mM, almost no inhibition of fetal enzyme
dCTP
-
-
dCTP
-
strong feedback inhibition, tight binding at the active site in stoichiometric amount
deoxcytidine
-
-
deoxcytidine
-
poor inhibitor
deoxyuridine
-
competitive to thymidine
deoxyuridine
-
-
dGDP
-
competitive to ATP
dTDP
-
competitive to ATP
dTDP
-
83% inhibition at 0.1 mM
dTMP
-
competitive to thymidine
dTMP
-
competitive to ATP
dTMP
-
-
dTMP
-
8% inhibition at 0.1 mM
dTTP
-
50-90% inhibition at 0.2-0.8 mM; competitive to thymidine
dTTP
-
non-competitive
dTTP
-
non-competitive with thymidine, competitive to ATP
dTTP
-
inhibition dependent on pH
dTTP
-
-
dTTP
-
84% inhibition at 0.1 mM
dTTP
-
competitive feedback inhibition
dTTP
-
strong feedback inhibition, tight binding at the active site in stoichiometric amount
dTTP
-
feedback inhibitor, binds tightly to the enzyme at the active site
dTTP
-
binds to the active site
dTTP
binds to the active site
dTTP
binds to the active site
dTTP
-
competitive feedback inhibition, binding structure analysis
dUTP
-
competitive to ATP
EDTA
-
complete incactivation at 2.5 mM
iododeoxycytidine
Herpes simplex virus
-
-
m-Fluorothymine
-
-
Mg2+
-
non-competitive
N-(2-cyanoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: 0.46 mM
N-(2-cyanoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: above 0.5 mM
N-(2-cyanoethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: 0.353 mM
N-(2-cyanoethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: above 0.5 mM
N-(2-[[cyano(phenyl)methyl]amino]-2-oxoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: 0.318 mM
N-(2-[[cyano(phenyl)methyl]amino]-2-oxoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: above 0.5 mM
N-(3'-deoxy-beta-D-thymidin-3'-yl)-N'-(4-chloro-3-trifluoromethylphenyl)-benzylguanidine
-
-
-
N-(3'-deoxy-beta-D-thymidin-3'-yl)-N'-(4-chloro-3-trifluoromethylphenyl)-guanidine
-
-
-
N-(3'-deoxy-beta-D-thymidin-3'-yl)-N'-(4-chloro-3-trifluoromethylphenyl)-isopropylguanidine
-
-
-
N-(cyanomethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: 0.268 mM
N-(cyanomethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: above 0.5 mM
N-(diphenylmethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: 0.238 mM
N-(diphenylmethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: above 0.5 mM
N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: 0.038 mM
N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: 0.043 mM
N-methyl-4-[[[8-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)octyl]oxy](diphenyl)methyl]benzamide
-
-
N-methyl-4-[{[8-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)octyl]oxy}(diphenyl)methyl]benzamide
-
-
-
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-2,2-diphenylacetamide
-
IC50: 0.027 mM
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-2,2-diphenylacetamide
-
IC50: 0.115 mM
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-3-(trifluoromethyl)benzamide
-
IC50: 0.028 mM
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-3-(trifluoromethyl)benzamide
-
IC50: 0.072 mM
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-9H-xanthene-9-carboxamide
-
IC50: 0.033 mM
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-9H-xanthene-9-carboxamide
-
IC50: 0.131 mM
N-[2-[(diphenylmethyl)amino]-2-oxoethyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: 0.287 mM
N-[2-[(diphenylmethyl)amino]-2-oxoethyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: above 0.5 mM
N-[cyano(phenyl)methyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: 0.208 mM
N-[cyano(phenyl)methyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
IC50: 0.495 mM
N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: 0.075 mM
N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
-
IC50: 0.275 mM
N3-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-thymine
-
IC50: above 0.5 mM
p-Fluorothymine
-
-
-
P1-(Adenosine-5')-P3-(thymidine-5')-triphosphate
-
-
P1-(adenosine-5')-P4-(thymidine-5')-tetraphosphate
-
-
P1-(Adenosine-5')-P5-(thymidine-5')-pentaphosphate
-
-
P1-(Adenosine-5')-P6-(thymidine-5')-hexaphosphate
-
-
penciclovir
-
three PCV-resistant FHV-1 variants selected in vitro carry mutations in the TK gene. Penciclovir is a potent selective inhibitor of Feline herpesvirus-1. The virus-encoded thymidine kinase is an important determinant of the virus susceptibility to nucleoside analogues
potassium phosphate
-
80% inhibition at 50 mM
sorivudine
-
-
stavudine
-
-
sulfate
-
competitive inhibitor of ATP binding
theophylline
-
-
thymidine
-
-
thymidine
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type enzyme at 0.003 mM, 50% photoincorporation inhibition of C336Y mutant at 0.05 mM
thymidine
-
inhibition of deoxycytidine kinase activity
thymidylate
Herpes simplex virus
-
50% photoincorporation inhibition of wild-type and C336Y mutant enzyme at 0.0125 mM
TTP
-
83% inhibition at 1 mM, 43% inhibition at 0.1 mM, 15% inhibition at 0.01 mM
W7
-
slight inhibitory in presence of calmodulin
zidovudine
-
-
Zn2+
-
1.1 mM and 2.9 mM
additional information
-
bisubstrate analogs with adenosine and thymidine joined at their 5' positions by polyphorphoryl linkages of varying lengths can serve as inhibitors
-
additional information
-
overview: purine nucleotides, pyrimidine nucleotides
-
additional information
-
no inhibition: phenylmethylsulfonylfluoride
-
additional information
-
no inhibition: AMP, CMP, GMP, putrescine, spermine, spermidine
-
additional information
-
5'-derivatives of thymidine
-
additional information
-
5'-derivatives of thymidine
-
additional information
-
overview: deoxythymidine analogs
-
additional information
Herpes simplex virus
-
structural requirements of bromine substituted 5-heteroaromatic 2'-deoxyuridines as antiviral agents
-
additional information
-
inhibition potency of dNTPs in descending order: dTTP, dCTP, dATP, inhibitor binding structurally stabilizes the enzyme, induction of conformational changes upon ligand binding, overview
-
additional information
-
structure-aided design of a series of substituted 3'- or 5'-thiourea derivatives of beta- and alpha-thymidine, respectively, as thymidine monophosphate kinase inhibitors
-
additional information
Herpes simplex virus
-
synthesis of tricarbonyl rhenium and technetium complexes of a 5-carboxamide 5-ethyl-2'-deoxyuridine for selective inhibition of herpes simplex virus thymidine kinase
-
additional information
not inhibited by D-5-(bromovinyl)-2'-deoxyuridine, L-5-(bromovinyl)-2'-deoxyuridine, and 2-phenylamino-9-(4-hydroxybutyl)-6-oxopurine
-
additional information
-
at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3-azido-3'-deoxythmidine or 3'-fluorothymidine
-
additional information
-
at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3'-azido-3'-deoxythmidine or 3'-fluorothymidine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
-
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
-
3'-azido-3'-deoxythymidine
-
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
3'-deoxy-3'-fluorothymidine
-
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
-
3'-fluorothymidine
-
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
-
ATP
-
binding of ATP induces enzyme tetramerization, which leads to activation of the enzyme, the dimeric enzyme form is probably less active, 50-100% activation at up to 5 mM
bovine serum albumin
-
activation at 0.2 mg/ml
-
Calmodulin
-
stimulates the enzyme by 13.8%, might modify the activity, enzyme contains a calmodulin binding domain, Ca2+ is required for binding, compounds W7 and W13 prevent stimulation, overview
dCDP
-
activation
dCDP
-
dCDP activates the enzyme 2.5old
dCTP
-
activation
dCTP
-
dCTP is not a phosphate donor, but instead activates the enzyme 7fold
dithiothreitol
-
-
dithiothreitol
-
the enzyme is stimulated by dithiothreitol up to a concentration of 2 mM
GTP
-
activation
Hydroxymethyl-dCDP
-
activation
Hydroxymethyl-dCTP
-
activation
NaCl
-
weak stimulation
potassium acetate
-
weak stimulation
KCl
-
weak stimulation
additional information
-
Km values of allosteric regulators
-
additional information
-
the enzyme is not activated by dATP or dGTP
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
2',3'-didehydro-2',3'-dideoxythymidine
-
at pH 7.8 and 25C
0.004
2'-deoxycytidine
-
dNTP-free recombinant dimeric enzyme, pH 7.5, 25C
0.0049
2'-deoxycytidine
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25C
0.018
2'-deoxycytidine
-
tetrameric enzyme, pH 7.5, 25C
0.025
2'-deoxycytidine
-
dimeric enzyme, pH 7.5, 25C
0.0058
2'-deoxythymidine
-
dNTP-free recombinant dimeric enzyme, pH 7.5, 25C
0.0065
2'-deoxythymidine
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25C
0.016
2'-deoxythymidine
-
37C, pH 7.6
0.025
2'-deoxythymidine
-
pH 8.0, 37C
0.041
2'-deoxythymidine
-
37C, pH 7.6
0.05
2'-deoxythymidine
-
tetrameric enzyme, pH 7.5, 25C
0.054
2'-deoxythymidine
-
dimeric enzyme, pH 7.5, 25C
0.33
2'-deoxythymidine
-
37C, pH 7.6
0.032
3'-azido-2',3'-dideoxythymidine
-
at pH 7.8 and 25C
0.00038
3'-azido-3'-deoxythymidine
isoform TK1b, pH and temperature not specified in the publication
0.00043
3'-azido-3'-deoxythymidine
-
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.00052
3'-azido-3'-deoxythymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0027
3'-azido-3'-deoxythymidine
isoform TK1a, pH and temperature not specified in the publication
0.012
3'-azido-3'-deoxythymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.015
3'-azido-3'-deoxythymidine
-
37C, pH 7.6
0.018
3'-azido-3'-deoxythymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.033
3'-azido-3'-deoxythymidine
-
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.05
3'-azido-3'-deoxythymidine
-
37C, pH 7.6
0.204
3'-azido-3'-deoxythymidine
-
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.254
3'-azido-3'-deoxythymidine
-
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.406
3'-azido-3'-deoxythymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.6
3'-azido-3'-deoxythymidine
-
37C, pH 7.6
2.318
3'-azido-3'-deoxythymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0563
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0174
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0318
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0356
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0552
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0799
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0373
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.0552
3-[1(2)-propyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.04
5-fluoro-2'-deoxyuridine
-
at pH 7.8 and 25C
0.03
5-fluorodeoxyuridine
-
full-length enzyme, at pH 7.8 and 37C
0.0012
5-iodo-2'-deoxythymidine
-
-
0.011
aciclovir monophosphate
Herpes simplex virus
-
-
0.00337
acyclovir
Herpes simplex virus
-
37C, mutant SR26
0.0056
acyclovir
Herpes simplex virus
-
37C, mutant SR11
0.00979
acyclovir
Herpes simplex virus
-
37C, mutant SR39
0.417
acyclovir
Herpes simplex virus
-
37C, wild-type
0.0013
ATP
Herpes simplex virus
-
pH 7.6, 37C, mutant C336Y
0.0047
ATP
-
dimeric enzyme, pH 7.5, 25C
0.0109
ATP
-
tetrameric enzyme, pH 7.5, 25C
0.01163
ATP
-
37C
0.013
ATP
Herpes simplex virus
-
pH 7.6, with deoxycytidine
0.015
ATP
-
pH 8.5, 30C
0.021
ATP
Herpes simplex virus
-
pH 7.6, with deoxyuridine
0.025
ATP
-
wild-type, thymidine as substrate, pH 7.6
0.03
ATP
Herpes simplex virus
-
pH 7.6, 37C, wild-type
0.03
ATP
-
recombinant wild-type enzyme, pH 7.5, 37C
0.037
ATP
-
wild-type, deoxycytidine as substrate, pH 7.6
0.041
ATP
-
recombinant mutant T298S, pH 7.5, 37C
0.049
ATP
-
recombinant mutant G294A, pH 7.5, 37C
0.05
ATP
-
pH 7, 37C
0.059
ATP
-
I212 mutant, deoxycytidine as substrate, pH 7.6
0.07
ATP
-
cytosolic enzyme, pH 7.5, 37C
0.076
ATP
-
pH 8, 25C or 37C
0.08
ATP
-
H121N mutant, thymidine as substrate, pH 7.6
0.088
ATP
-
recombinant mutant K297R, pH 7.5, 37C
0.09
ATP
-
pH 7.4, 37C
0.095
ATP
-
I212 mutant, thymidine as substrate, pH 7.6
0.098
ATP
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37C
0.1
ATP
-
mitochondrial enzyme, pH 7.5, 37C
0.11
ATP
-
mitochondrial enzyme, pH 7.5, 37C
0.118
ATP
Herpes simplex virus
-
-
0.14
ATP
-
at pH 7.8 and 25C
0.15
ATP
-
pH 7.8, 37C
0.162
ATP
-
H121N mutant, deoxycytidine as substrate, pH 7.6
0.18
ATP
-
full-length enzyme, with 0.0005 mM thymidine as cosubstrate, at pH 7.8 and 37C
0.2
ATP
-
pH 8, 37C
0.21
ATP
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37C
0.22
ATP
-
full-length enzyme, with 0.005 mM thymidine as cosubstrate, at pH 7.8 and 37C
0.3
ATP
-
GTP
0.32
ATP
-
full-length enzyme, with 0.5 mM deoxyinosine as cosubstrate, at pH 7.8 and 37C
2.6
ATP
-
at concentrations above 1 mM, pH 8, 37C
20
ATP
-
at concentrations below 1 mM, pH 8, 37C
0.29
beta-L-thymidine
-
at pH 7.8 and 25C
0.049
CTP
-
recombinant wild-type enzyme, pH 7.5, 37C
0.057
CTP
-
about, recombinant mutant T298S, pH 7.5, 37C
0.088
CTP
-
recombinant mutant G294A, pH 7.5, 37C
0.108
CTP
-
recombinant mutant K297R, pH 7.5, 37C
0.15
CTP
-
pH 7.4, 37C
0.15
CTP
-
ATP
8.4
deoxyadenosine
-
full-length enzyme, at pH 7.8 and 37C
0.003
deoxycytidine
-
mitochondrial enzyme, pH 7.5, 37C
0.0036
deoxycytidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.006
deoxycytidine
-
pH 7.4, 37C
0.011
deoxycytidine
-
wild-type and H121N mutant, pH 7.6
0.0115
deoxycytidine
mTK2-55 pH 7.6
0.0236
deoxycytidine
mTK2-39 pH 7.6
0.0251
deoxycytidine
mTK2 pH 7.6
0.0414
deoxycytidine
-
pH and temperature not specified in the publication
0.106
deoxycytidine
Herpes simplex virus
-
pH 7.6
0.568
deoxycytidine
-
I212 mutant, pH 7.6
2.6
deoxyguanosine
-
full-length enzyme, at pH 7.8 and 37C
2.5
deoxyinosine
-
full-length enzyme, at pH 7.8 and 37C
0.0047
deoxyuridine
Herpes simplex virus
-
pH 7.6
0.0066
deoxyuridine
isoform TK1b, pH and temperature not specified in the publication
0.015
deoxyuridine
isoform TK1a, pH and temperature not specified in the publication
0.08
deoxyuridine
-
full-length enzyme, at pH 7.8 and 37C
0.118
deoxyuridine
-
at pH 7.8 and 25C
0.43
deoxyuridine
-
thymidine kinase domain 2, at pH 7.8 and 37C
0.066
difluorodeoxycytidine
-
-
0.029
difluorodeoxyuridine
-
-
0.00333
ganciclovir
Herpes simplex virus
-
37C, mutant SR39
0.00614
ganciclovir
Herpes simplex virus
-
37C, mutant SR11
0.01755
ganciclovir
Herpes simplex virus
-
37C, mutant SR26
0.0476
ganciclovir
Herpes simplex virus
-
37C, wild-type
0.05
ganciclovir
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125E
0.069
ganciclovir
Herpes simplex virus
-
pH 7.6, 37C, wild-type
0.473
ganciclovir
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125N
0.12
ganciclovir monophosphate
Herpes simplex virus
-
-
0.041
GTP
-
recombinant wild-type enzyme, pH 7.5, 37C
0.043
GTP
-
about, recombinant mutant K297R, pH 7.5, 37C
0.045
GTP
-
recombinant mutant T298S, pH 7.5, 37C
0.069
GTP
-
recombinant mutant G294A, pH 7.5, 37C
0.3
GTP
-
pH 7.4, 37C
0.3
GTP
-
pH 7.8, 37C
0.0001
MgATP2-
-
two active sites for ATP
0.0028
MgATP2-
-
dNTP-free recombinant dimeric enzyme, pH 7.5, 25C
0.0059
MgATP2-
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25C
0.9
MgATP2-
-
two active sites for ATP
0.00015
thymidine
-
pH 8.5, 30C
0.0002
thymidine
Herpes simplex virus
-
pH 7.2, wild-type
0.00023
thymidine
-
mutant enzyme P12N/H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.00027
thymidine
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0003
thymidine
-
37C
0.0003
thymidine
-
pH 7.5, recombinant mutants V106A and V106G, in presence of ATP; pH 7.5, recombinant mutant V106H, in absence of ATP
0.0003
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00038
thymidine
Herpes simplex virus
-
37C, wild-type
0.0004
thymidine
-
pH 7.5, recombinant mutants V106L and V106G, in absence of ATP; pH 7.5, recombinant mutants V106L, V106H and V106T, in presence of ATP
0.00044
thymidine
-
wild type enzyme, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.0005
thymidine
-
pH 8
0.0005
thymidine
-
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00054
thymidine
-
pH 8.0, 37C, recombinant wild-type enzyme
0.0006
thymidine
Herpes simplex virus
-
-
0.0006
thymidine
-
pH 7.5, recombinant mutant V106M, in absence or presence of ATP; pH 7.5, recombinant wild-type enzyme, in presence of ATP
0.0006
thymidine
Q9S750 and Q9FN47
subunit alpha, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00062
thymidine
isoform TK1a, pH and temperature not specified in the publication
0.0007
thymidine
-
TK-A, below 0.004 mM deoxythymidine, 25C
0.0007
thymidine
-
pH 7.5, recombinant mutant V106K, in absence of ATP
0.0007
thymidine
Q9S750 and Q9FN47
subunit alpha, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0007
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0008
thymidine
-
pH 7.5, recombinant mutant V106Q, in absence or presence of ATP
0.0009
thymidine
Herpes simplex virus
-
pH 7.6, 37C, wild-type
0.0009
thymidine
-
pH 7.5, recombinant mutant V106I, in presence of ATP
0.0009
thymidine
Q9S750 and Q9FN47
subunit beta, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication; subunit beta, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0009
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00095
thymidine
Herpes simplex virus
-
37C, mutant SR11
0.001
thymidine
-
TK-C, below 0.004 mM deoxythymidine, 25C
0.0012
thymidine
-
pH 7.5, recombinant mutant V106K, in presence of ATP
0.0012
thymidine
-
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0013
thymidine
Herpes simplex virus
-
37C, mutant SR26
0.0013
thymidine
Herpes simplex virus
-
pH 7.6, 37C, wild-type
0.0013
thymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0014
thymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0014
thymidine
isoform TK1b, pH and temperature not specified in the publication
0.0014
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0016
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0017
thymidine
-
-
0.002
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0022
thymidine
-
pH 8, 37C
0.0023
thymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0024
thymidine
-
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0025
thymidine
Herpes simplex virus
-
pH 7.2, Y101F mutant
0.0025
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0026
thymidine
-
cytoplasmic enzyme, pH 7.5, 37C
0.0026
thymidine
-
mitochondrial enzyme, pH 7.5, 37C
0.00264
thymidine
Herpes simplex virus
-
37C, mutant SR39
0.003
thymidine
-
pH 7.4
0.003
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125N
0.0032
thymidine
-
-
0.0034
thymidine
mTK2 pH 7.6
0.0039
thymidine
-
-
0.0039
thymidine
-
thymidine kinase-2 mutant enzyme I59Y, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.0039
thymidine
-
mutant enzyme P12N, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.0041
thymidine
-
pH 8, 25C or 37C
0.0043
thymidine
-
wild type thymidine kinase-2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.0049
thymidine
mTK2-39 pH 7.6
0.0049
thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.005 - 0.0052
thymidine
-
mitochondrial enzyme, pH 7.5, 37C
0.005
thymidine
-
pH 7.4, 37C
0.0051
thymidine
-
thymidine kinase-2 mutant enzyme I59V, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.0056
thymidine
-
pH 8.0, 37C, Km is markedly dependent on pH
0.0056
thymidine
-
thymidine kinase-2 mutant enzyme I59L, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.0057
thymidine
-
H121N mutant, pH 7.6
0.00596
thymidine
-
pH 8.0, 37C, recombinant mutant S13D
0.006
thymidine
-
thymidine kinase-2 mutant enzyme I59H, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.007
thymidine
-
TK-A, above 0.004 mM deoxythymidine, 25C
0.0073
thymidine
mTK2-55 pH 7.6
0.0078
thymidine
-
full-length enzyme, at pH 7.8 and 37C
0.008
thymidine
-
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.009
thymidine
-
pH 7.8, 37C
0.01
thymidine
-
cytosolic enzyme, pH 7.5, 37C
0.01
thymidine
-
TK-C, above 0.004 mM deoxythymidine, 25C
0.01
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant C336Y
0.012
thymidine
-
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0127
thymidine
-
pH 7.5, recombinant mutant V106A, in absence of ATP
0.013
thymidine
-
wild-type, pH 7.6
0.016
thymidine
-
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.017
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.017
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.018
thymidine
-
I212 mutant, pH 7.6
0.0188
thymidine
-
pH and temperature not specified in the publication
0.02
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125E
0.0271
thymidine
-
pH 7.5, recombinant wild-type enzyme, in absence of ATP
0.0294
thymidine
-
pH 7.5, recombinant mutant V106I, in absence of ATP
0.036
thymidine
-
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.037
thymidine
-
mutant enzyme H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.043
thymidine
-
pH 7.5, recombinant mutant V106T, in absence of ATP
0.046
thymidine
-
thymidine kinase domain 2, at pH 7.8 and 37C
0.048
thymidine
-
at pH 7.8 and 25C
0.057
thymidine
-
pH 7, 37C
0.059
thymidine
-
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.074
thymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.263
thymidine
-
mutant enzyme P12N/H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.317
thymidine
-
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.336
thymidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.349
thymidine
-
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.565
thymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.59
thymidine
-
mutant enzyme P12N/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.612
thymidine
-
mutant enzyme H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
6
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125D
0.022
trifluoromethylthymidine
-
at pH 7.8 and 25C
0.072
TTP
-
recombinant wild-type enzyme, pH 7.5, 37C
0.127
TTP
-
recombinant mutant T298S, pH 7.5, 37C
7.8
uridine
-
full-length enzyme, at pH 7.8 and 37C
0.26
UTP
-
pH 7.4, 37C
0.8
MgCl2
-
pH 8
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics, ATP-binding kinetics of wild-type and mutant enzymes at room temperature
-
additional information
additional information
-
steady-state kinetics, kinetics of conformational changes induced by ligand binding
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes in absence or presence of ATP, overview
-
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0253
(E)-5-(2-bromovinyl)-2'-deoxyuridine
Herpes simplex virus
-
determined by capillary electrophoresis method
0.2
2'-deoxythymidine
Vaccinia virus
-
pH 8.0, 37C
6.47
2'-deoxythymidine
Canid herpesvirus 1
-
37C, pH 7.6
23
2'-deoxythymidine
Felid herpesvirus 1
-
37C, pH 7.6
220
2'-deoxythymidine
Vaccinia virus
-
37C, pH 7.6
0.17
3'-azido-2',3'-dideoxythymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
0.009
3'-azido-3'-deoxythymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.13
3'-azido-3'-deoxythymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.24
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.34
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.53
3'-azido-3'-deoxythymidine
Canid herpesvirus 1
-
37C, pH 7.6
0.58
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.81
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.97
3'-azido-3'-deoxythymidine
Felid herpesvirus 1
-
37C, pH 7.6
1.3
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.4
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.5
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
3.5
3'-azido-3'-deoxythymidine
Homo sapiens
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
9.3
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
412
3'-azido-3'-deoxythymidine
Vaccinia virus
-
37C, pH 7.6
0.47
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.32
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.44
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.35
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.39
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.37
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.45
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.34
3-[1(2)-propyltetrazol-5-yl]thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.24
5-fluoro-2'-deoxyuridine
Cryptosporidium parvum
-
at pH 7.8 and 25C
3.6
5-fluorodeoxyuridine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
0.238
aciclovir monophosphate
Herpes simplex virus
-
determined by capillary electrophoresis method
0.000283
acyclovir
Herpes simplex virus
-
37C, mutant SR26
0.000433
acyclovir
Herpes simplex virus
-
37C, mutant SR39
0.000467
acyclovir
Herpes simplex virus
-
37C, mutant SR11
0.015
acyclovir
Herpes simplex virus
-
37C, wild-type
0.28
ATP
Cryptosporidium parvum
-
at pH 7.8 and 25C
2 - 8
ATP
Human herpesvirus 4
-
recombinant wild-type enzyme, pH 7.5, 37C
3.1
ATP
Trypanosoma brucei
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37C
3.9
ATP
Trypanosoma brucei
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37C
7
ATP
Human herpesvirus 4
-
recombinant mutant K297R, pH 7.5, 37C
11
ATP
Human herpesvirus 4
-
recombinant mutant G294A, pH 7.5, 37C
24
ATP
Human herpesvirus 4
-
recombinant mutant T298S, pH 7.5, 37C
0.007
beta-L-thymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
9
CTP
Human herpesvirus 4
-
recombinant mutant G294A, pH 7.5, 37C
13.5
CTP
Human herpesvirus 4
-
about, recombinant mutant T298S, pH 7.5, 37C
14
CTP
Human herpesvirus 4
-
recombinant mutant K297R, pH 7.5, 37C
19
CTP
Human herpesvirus 4
-
recombinant wild-type enzyme, pH 7.5, 37C
0.047
deoxyadenosine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
0.17
deoxycytidine
Caenorhabditis elegans
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.4
deoxycytidine
Herpes simplex virus
-
pH 7.6
0.34
deoxyguanosine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
0.8
deoxyinosine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
0.045
deoxyuridine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.53
deoxyuridine
Cryptosporidium parvum
-
at pH 7.8 and 25C
1.3
deoxyuridine
Herpes simplex virus
-
pH 7.6
1.5
deoxyuridine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
5.1
deoxyuridine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
5.3
deoxyuridine
Trypanosoma brucei
-
thymidine kinase domain 2, at pH 7.8 and 37C
0.0035
ganciclovir
Herpes simplex virus
-
37C, mutant SR39
0.0056
ganciclovir
Herpes simplex virus
-
37C, mutant SR11
0.0089
ganciclovir
Herpes simplex virus
-
37C, mutant SR26
0.04
ganciclovir
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125N
0.08
ganciclovir
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125E
0.1
ganciclovir
Herpes simplex virus
-
37C, wild-type
0.47
ganciclovir
Herpes simplex virus
-
pH 7.6, 37C, wild-type
0.0272
ganciclovir monophosphate
Herpes simplex virus
-
determined by capillary electrophoresis method
8
GTP
Human herpesvirus 4
-
recombinant mutant G294A, pH 7.5, 37C
17
GTP
Human herpesvirus 4
-
recombinant mutant T298S, pH 7.5, 37C
18.5
GTP
Human herpesvirus 4
-
about, recombinant mutant K297R, pH 7.5, 37C
0.003
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125N
0.0062
thymidine
Herpes simplex virus
-
37C, mutant SR26
0.007
thymidine
Herpes simplex virus
-
37C, mutant SR11
0.017
thymidine
Herpes simplex virus
-
37C, mutant SR39
0.02
thymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.06
thymidine
Herpes simplex virus
-
pH 7.6, 37C, wild-type
0.11
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125D
0.13
thymidine
Herpes simplex virus
-
pH 7.6, 37C, mutant Q125E
0.28
thymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
0.46
thymidine
Herpes simplex virus
-
37C, wild-type
0.46
thymidine
Homo sapiens
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37C
0.48
thymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.74
thymidine
Caenorhabditis elegans
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.97
thymidine
Caenorhabditis elegans
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.4
thymidine
Homo sapiens
-
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.5
thymidine
Homo sapiens
-
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.8
thymidine
Homo sapiens
-
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
2.7
thymidine
Homo sapiens
-
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
2.8
thymidine
Caenorhabditis elegans
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
3.1
thymidine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
3.5
thymidine
Trypanosoma brucei
-
thymidine kinase domain 2, at pH 7.8 and 37C
4.9
thymidine
Caenorhabditis elegans
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
6.4
thymidine
Caenorhabditis elegans
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
9.5
thymidine
Homo sapiens
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
265.5
thymidine
Homo sapiens
-
pH 8.0, 37C, recombinant mutant S13D
282
thymidine
Homo sapiens
-
pH 8.0, 37C, recombinant wild-type enzyme
0.36
trifluoromethylthymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
7
TTP
Human herpesvirus 4
-
recombinant mutant T298S, pH 7.5, 37C
11
TTP
Human herpesvirus 4
-
recombinant wild-type enzyme, pH 7.5, 37C
1.5
uridine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
0.03
2',3'-didehydro-2',3'-dideoxythymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
additional information
2'-deoxythymidine
Canid herpesvirus 1
-
37C, pH 7.6
21
GTP
Human herpesvirus 4
-
recombinant wild-type enzyme, pH 7.5, 37C
additional information
additional information
Homo sapiens
-
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.064
2',3'-didehydro-2',3'-dideoxythymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
28913
5
3'-azido-2',3'-dideoxythymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
4954
0.0031
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
0.0067
3'-azido-3'-deoxythymidine
Homo sapiens
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
0.078
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
0.35
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
0.44
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
0.49
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
0.94
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
1.4
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
1.7
3'-azido-3'-deoxythymidine
Homo sapiens
-
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
22
3'-azido-3'-deoxythymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
1185
48
3'-azido-3'-deoxythymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
1185
98
3'-azido-3'-deoxythymidine
Caenorhabditis elegans
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1185
5.8
5-fluoro-2'-deoxyuridine
Cryptosporidium parvum
-
at pH 7.8 and 25C
3318
120
5-fluorodeoxyuridine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
3977
2.3
ATP
Cryptosporidium parvum
-
at pH 7.8 and 25C
4
15
ATP
Trypanosoma brucei
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37C
4
40
ATP
Trypanosoma brucei
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37C
4
0.0024
beta-L-thymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
14484
0.0056
deoxyadenosine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
601
0.0019
deoxycytidine
Caenorhabditis elegans
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
451
0.048
deoxycytidine
Caenorhabditis elegans
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
451
0.0066
deoxyguanosine
Caenorhabditis elegans
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
732
0.13
deoxyguanosine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
732
0.23
deoxyguanosine
Caenorhabditis elegans
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
732
0.32
deoxyinosine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
1104
4.5
deoxyuridine
Cryptosporidium parvum
-
at pH 7.8 and 25C
1060
6.8
deoxyuridine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
1060
12
deoxyuridine
Trypanosoma brucei
-
thymidine kinase domain 2, at pH 7.8 and 37C
1060
63
deoxyuridine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
1060
150
deoxyuridine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
1060
0.0028
thymidine
Caenorhabditis elegans
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
0.0068
thymidine
Homo sapiens
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
0.46
thymidine
Homo sapiens
-
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
0.51
thymidine
Homo sapiens
-
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
4.2
thymidine
Homo sapiens
-
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
4.5
thymidine
Homo sapiens
-
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
4.8
thymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
166
5
thymidine
Caenorhabditis elegans
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
10
thymidine
Caenorhabditis elegans
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
14
thymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
166
15
thymidine
Caenorhabditis elegans
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
76
thymidine
Trypanosoma brucei
-
thymidine kinase domain 2, at pH 7.8 and 37C
166
400
thymidine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
166
750
thymidine
Caenorhabditis elegans
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
166
770
thymidine
Arabidopsis thaliana
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
166
6.4
trifluoromethylthymidine
Cryptosporidium parvum
-
at pH 7.8 and 25C
42444
0.19
uridine
Trypanosoma brucei
-
full-length enzyme, at pH 7.8 and 37C
261
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.003
2'-deoxy-3'-aminothymidine
-
pH 7.8, 37C
0.011
2'-exo-methanocarba-thymidine
Herpes simplex virus
-
pH 7.2, wild-type
0.0515
2'-exo-methanocarba-thymidine
Herpes simplex virus
-
pH 7.2, Y101F mutant
0.0039
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
-
pH and temperature not specified in the publication
-
0.0014
3'-deoxy-3'-fluorothymidine
-
pH and temperature not specified in the publication
-
0.0014
3'-fluorothymidine
-
pH and temperature not specified in the publication
-
1.7
3-N-methyl-5-iodo-2'-deoxyuridine
-
pH 7.8, 37C
3
5'-aminothymidine
-
pH 7.8, 37C
0.0104
5-(E)-(2-bromovinyl)uridine
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.00013
5-bromodeoxyuridine
-
pH 8.5, 30C
0.0015 - 0.0055
5-bromodeoxyuridine
-
pH 7.5, 27C
0.00072
5-fluorodeoxyuridine
-
pH 8.5, 30C
0.022
5-fluorodeoxyuridine
-
pH 8, 37C
0.06 - 0.096
5-fluorodeoxyuridine
-
pH 7.5, 27C
0.001 - 0.0045
5-iododeoxyuridine
-
pH 7.5, 27C
0.3
acyclovir
Herpes simplex virus
-
pH 7.8, 37C
0.4
ADP
-
with ATP as variable substrate, pH 8, 37C
6.5
ADP
-
pH 7.4, 37C
3.8
ATP
-
pH 7, 37C
0.001
Be2+
-
pH 8, 37C
7.5
Caffeine
-
pH 7.8, 37C
0.00036
dCTP
-
H121N mutant, pH 7.6, competitive with thymidine as substrate
0.00079
dCTP
-
wild-type enzyme, pH 7.6, competitive with thymidine as substrate
0.00087
dCTP
-
wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate
0.00089
dCTP
-
H121N mutant, pH 7.6, competitive with deoxycytidine as substrate
0.002
dCTP
-
pH 7.5, 37C
0.004
deoxycytidine
-
H121N mutant, pH 7.6, non-competitive with thymidine as substrate
0.04
deoxycytidine
-
wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate
0.63
deoxycytidine
-
-
0.00146
deoxyuridine
-
pH 8.5, 30C
0.11
deoxyuridine
-
pH 8, 37C
0.27 - 1.54
deoxyuridine
-
pH 7.5, 27C
0.5 - 0.65
dTDP
-
pH 7.5, 27C
0.65
dTMP
-
with ATP or thymidine as variable substrate, pH 8, 37C
3.6 - 4.7
dTMP
-
pH 7.5, 27C
0.0002
dTTP
-
variant TK-A, 25C
0.0006
dTTP
-
variant TK-C, 25C
0.00089
dTTP
-
H121N mutant, pH 7.6, competitive with thymidine as substrate
0.002
dTTP
-
wild-type enzyme, pH 7.6, competitive with thymidine as substrate
0.0025
dTTP
-
wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate
0.006
dTTP
-
pH 7, 37C
0.01
dTTP
-
pH 7.5, 37C
0.014 - 0.036
dTTP
-
pH 7.5, 27C
0.014
iododeoxycytidine
Herpes simplex virus
-
pH 7.8, 37C
0.0036
thymidine
-
H121N mutant, pH 7.6, competitive with deoxycytidine as substrate
0.0049
thymidine
-
wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate
0.006
thymidine
-
-
0.63
TMP
-
pH 7.4, 37C
0.0085
TTP
-
competitive to ATP, pH 7.4, 37C
0.0135
TTP
-
competitive to thymidine, pH 7.4, 37C
2.5
Mg2+
-
pH 7, 37C
additional information
additional information
-
bisubstrate analogs with adenosine and thymidine joined at their 5' positions by polyphorphoryl linkages of varying lengths
-
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.028
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl 3,3-dimethylbutanoate
Homo sapiens
-
IC50: 0.028 mM
0.5
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl 3,3-dimethylbutanoate
Human herpesvirus 1
-
IC50: above 0.5 mM
0.03
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl benzoate
Homo sapiens
-
IC50: 0.03 mM
0.5
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl benzoate
Human herpesvirus 1
-
IC50: above 0.5 mM
0.0046
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl diphenylacetate
Homo sapiens
-
IC50: 0.0046 mM
0.281
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl diphenylacetate
Human herpesvirus 1
-
IC50: 0.281 mM
0.019
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl dodecanoate
Homo sapiens
-
IC50: 0.019 mM
0.5
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl dodecanoate
Human herpesvirus 1
-
IC50: above 0.5 mM
0.011
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl heptanoate
Homo sapiens
-
IC50: 0.011 mM
0.42
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl heptanoate
Human herpesvirus 1
-
IC50: 0.42 mM
0.04
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl pivalate
Homo sapiens
-
IC50: 0.04 mM
0.5
(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl pivalate
Human herpesvirus 1
-
IC50: above 0.5 mM
0.265
(4E)-N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
Homo sapiens
-
IC50: 0.265 mM
0.5
(4E)-N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.371
(4E)-N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
Homo sapiens
-
IC50: 0.371 mM
0.5
(4E)-N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hex-4-enamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.043
(E)-5-(2-bromovinyl)-1-beta-D-(arabinofuranosyl)uracil
Homo sapiens
-
-
0.5
1-(3'-deoxy-5'-O-triphenylmethyl-beta-D-thymidin-3'-yl)-3-(4-chloro-3-(trifluoromethyl)-phenyl)-thiourea
Homo sapiens
-
IC50 above 0.5 mM, thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.041
1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Homo sapiens
-
-
0.365
1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Human herpesvirus 1
-
-
0.057
1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Homo sapiens
-
-
0.422
1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Human herpesvirus 1
-
-
0.099
1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Homo sapiens
-
-
0.5
1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Human herpesvirus 1
-
IC50 above 0.5 mM
0.285
1-beta-D-(arabinofuranosyl)thymine
Homo sapiens
-
-
0.18
1-[(2Z)-4-(4-benzylpiperazin-1-yl)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens, Human herpesvirus 1
-
IC50: 0.18 mM
0.057
1-[(2Z)-4-(benzylamino)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens, Human herpesvirus 1
-
IC50: 0.057 mM
0.022
1-[(2Z)-4-(benzyloxy)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.022 mM
0.5
1-[(2Z)-4-(benzyloxy)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: above 0.5 mM
0.0035
1-[(2Z)-4-(dibenzylamino)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens, Human herpesvirus 1
-
IC50: 0.0035 mM
0.5
1-[(2Z)-4-(dibenzylamino)but-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: above 0.5 mM
0.01
1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-5,6-dihydrothymine
Homo sapiens
-
IC50: 0.01 mM
0.5
1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-5,6-dihydrothymine
Human herpesvirus 1
-
IC50: above 0.5 mM
0.173
1-[(2Z)-4-hydroxybut-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.173 mM
0.224
1-[(2Z)-4-hydroxybut-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.224 mM
0.0015
1-[(Z)-4-(triphenylmethoxy)-2-butenyl]thymine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.013
1-[2,3-dihydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.013 mM
0.017
1-[2,3-dihydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.017 mM
0.0012
1-[3-hydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.0012 mM
0.0036
1-[3-hydroxy-4-(trityloxy)butyl]-5-methylpyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.0036 mM
0.00015
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea
Homo sapiens
-
-
0.195
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea
Human herpesvirus 1
-
-
0.00042
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea
Homo sapiens
-
-
0.335
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea
Human herpesvirus 1
-
-
0.038
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Homo sapiens
-
-
0.472
1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea
Human herpesvirus 1
-
-
0.405
1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea
Homo sapiens
-
-
0.5
1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea
Human herpesvirus 1
-
IC50 above 0.5 mM
0.023
2-(4-chlorophenyl)-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
Homo sapiens
-
IC50: 0.023 mM
0.198
2-(4-chlorophenyl)-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
Human herpesvirus 1
-
IC50: 0.198 mM
0.0041
2-biphenyl-4-yl-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
Homo sapiens
-
IC50: 0.0041 mM
0.119
2-biphenyl-4-yl-N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]acetamide
Human herpesvirus 1
-
IC50: 0.119 mM
0.0047
3'-(1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.06
3'-(4-(2-phenyl-ethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.12
3'-(4-(2-phenyl-ethyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.053
3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.211
3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-2'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.105
3'-(4-(3-chloro-propyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.208
3'-(4-(3-chloro-propyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.044
3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.071
3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-2'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.0003
3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.073
3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.078
3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.00023
3'-(4-butyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.0033
3'-(4-chlorobenzylamino)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.000036
3'-(4-chlorophenyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.000046
3'-(4-chlorophenyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.00015
3'-(4-cyclopentylmethyl-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.086
3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.15
3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.099
3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.2
3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.189
3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.311
3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.114
3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.318
3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
-
0.004
3'-(5-(4-chlorophenyl)-1,2,3-triazol-1-yl)-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.0012
3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine
Homo sapiens
-
-
0.4
3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine
Human herpesvirus 1
-
-
0.000036
3'-([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)-2',3'-dideoxy-3,4-dihydrothymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
0.00015
3'-([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)-2',3'-dideoxy-3,4-dihydrothymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
0.003
3'-azido-3'-deoxythymidine
Homo sapiens
-
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
0.0125
3'-azido-3'-deoxythymidine
Ureaplasma parvum
Q9PPP5
in 50 mM Tris/HCl pH 7.6, 5 mM MgCl2, at 37C
0.0046
3'-benzylamino-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.000042
3'-deoxy-3'-(4-(3,4-dichlorophenyl)-1,2,3-triazol-1-yl)-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.00025
3'-deoxy-3'-(4-phenyl-1,2,3-triazol-1-yl)-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.00032
3'-deoxy-3'-(4-phenyl-1,2,3-triazol-1-yl)-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.0013
3'-deoxy-3'-(4-pyridin-2-yl-1,2,3-triazol-1-yl)-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.0011
3'-deoxy-3'-(5-phenyl-1,2,3-triazol-1-yl)-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37C
-
0.00064
3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine
Homo sapiens
-
-
0.087
3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine
Human herpesvirus 1
-
-
0.0031
3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine
Homo sapiens
-
-
0.5
3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine
Human herpesvirus 1
-
IC50 above 0.5 mM
0.0012
3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine
Homo sapiens
-
-
0.444
3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine
Human herpesvirus 1
-
-
0.018
3'-ethyl-5-methyl-2'-deoxyuridine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0046
3'-spiro-(4''-amino-1'',2''-oxathiole-2'',2''-dioxide)-5-methyluridine
Homo sapiens
-
inhibits phosphorylation of 2'-deoxythymidine, IC50: 0.0046 mM
0.00033
3'-[(3-trifluoromethyl-4-chloro)-benzylamino]-3'-deoxy-beta-D-thymidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.00043
3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine
Homo sapiens
-
-
0.232
3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine
Human herpesvirus 1
-
-
0.00047
3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine
Homo sapiens
-
-
0.046
3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine
Human herpesvirus 1
-
-
0.175
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylbutanamide
Human herpesvirus 1
-
IC50: 0.175 mM
0.44
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylbutanamide
Homo sapiens
-
IC50: 0.44 mM
0.191
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-[2-oxo-2-(tritylamino)ethyl]butanamide
Human herpesvirus 1
-
IC50: 0.191 mM
0.194
4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-[2-oxo-2-(tritylamino)ethyl]butanamide
Homo sapiens
-
IC50: 0.194 mM
0.031
5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine
Homo sapiens
-
-
0.392
5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine
Human herpesvirus 1
-
-
0.11
5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine
Homo sapiens
-
-
0.5
5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine
Human herpesvirus 1
-
IC50 above 0.5 mM
0.468
5'-O-(4,4'-dimethoxytrityl)thymidine
Homo sapiens
-
IC50: 0.468 mM
0.000014
5-(3'-amino-3'-deoxy-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridin-3'Nyl)-1-(4-chloro-3-trifluoromethylphenyl)-tetrazole
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.000035
5-(3'-amino-3'-deoxy-beta-D-thymidin-3'N-yl)-1-(4-chloro-3-trifluoromethylphenyl)-tetrazole
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.0009
5-(3'-amino-3'-deoxy-beta-D-thymidin-3'N-yl)-1-benzyl-tetrazole
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.0013
5-(E)-(2-bromovinyl)-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Homo sapiens
-
IC50: 0.0013 mM
0.1
5-(E)-(2-bromovinyl)-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Homo sapiens
-
IC50: 0.1 mM
0.5
5-(E)-(2-bromovinyl)-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Human herpesvirus 1
-
IC50: above 0.5 mM
0.0026
5-aminobenzyl-(3'-deoxy-beta-D-thymidin-3'N-yl)-tetrazole
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.02
5-ethyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Homo sapiens
-
IC50: 0.02 mM
0.5
5-ethyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Human herpesvirus 1
-
IC50: above 0.5 mM
0.0046
5-iodo-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Homo sapiens
-
IC50: 0.0046 mM
0.048
5-iodo-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-uracil
Human herpesvirus 1
-
IC50: 0.048 mM
0.001
5-iodo-2'-deoxyuridine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.003
5-methyl-1-[(2E)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.003 mM
0.025
5-methyl-1-[(2E)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.025 mM
0.491
5-methyl-1-[(2Z)-4-(4-methylpiperazin-1-yl)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens, Human herpesvirus 1
-
IC50: 0.491 mM
0.0023
5-methyl-1-[(2Z)-4-(tritylamino)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens, Human herpesvirus 1
-
IC50: 0.0023 mM
0.5
5-methyl-1-[(2Z)-4-(tritylamino)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: above 0.5 mM
0.484
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-cytosine
Homo sapiens
-
IC50: 0.484 mM
0.5
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-cytosine
Human herpesvirus 1
-
IC50: above 0.5 mM
0.0015
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.0015 mM
0.045
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.045 mM
0.1
5-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: above 0.1 mM
0.0031
5-methyl-1-[4-(trityloxy)but-2-yn-1-yl]pyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.0031 mM
0.404
5-methyl-1-[4-(trityloxy)but-2-yn-1-yl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.404 mM
0.0033
5-methyl-1-[4-(trityloxy)butyl]pyrimidine-2,4(1H,3H)-dione
Homo sapiens
-
IC50: 0.0033 mM
0.01
5-methyl-1-[4-(trityloxy)butyl]pyrimidine-2,4(1H,3H)-dione
Human herpesvirus 1
-
IC50: 0.01 mM
0.0444
5-propyl-2'-deoxyuridine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0006
5-trifluoromethyl-2-deoxyuridine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0012
5-[(E)-2-bromoethenyl]-2'-O-decanoyluridine
Homo sapiens
-
competitive with respect to 2'-deoxythymidine, IC50: 0.0012 mM
0.00015
5-[(E)-2-bromoethenyl]-2'-O-octanoyluridine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
0.006
5-[(E)-2-bromoethenyl]-2'-O-octanoyluridine
Homo sapiens
-
IC50: 0.006 mM
0.0004
5-[amino-(4-chloro-3-trifluoromethylphenyl)]-1-(3'-deoxy-beta-(E)-5-(2-bromovinyl)-2'-deoxyuridin-3'N-yl)-tetrazole
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.00059
5-[amino-(4-chloro-3-trifluoromethylphenyl)]-1-(3'-deoxy-beta-D-thymidin-3'N-yl)-tetrazole
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.0034
6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylhexanamide
Human herpesvirus 1
-
IC50: 0.0034 mM
0.019
6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-tritylhexanamide
Homo sapiens
-
IC50: 0.019 mM
0.015
6-methyluridine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.5
9-[(2Z)-4-(trityloxy)but-2-en-1-yl]-guanine
Homo sapiens, Human herpesvirus 1
-
IC50: above 0.5 mM
0.017
alpha-D-5-ethyl-2'-deoxyuridine
Entamoeba histolytica
Q6S4W2
-
0.035
alpha-L-thymidine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0025
beta-D-5-ethyl-2'-deoxyuridine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.005
beta-L-thymidine
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.46
N-(2-cyanoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Homo sapiens
-
IC50: 0.46 mM
0.5
N-(2-cyanoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.353
N-(2-cyanoethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Homo sapiens
-
IC50: 0.353 mM
0.5
N-(2-cyanoethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.318
N-(2-[[cyano(phenyl)methyl]amino]-2-oxoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Homo sapiens
-
IC50: 0.318 mM
0.5
N-(2-[[cyano(phenyl)methyl]amino]-2-oxoethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.0013
N-(3'-deoxy-beta-D-thymidin-3'-yl)-N'-(4-chloro-3-trifluoromethylphenyl)-benzylguanidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.00043
N-(3'-deoxy-beta-D-thymidin-3'-yl)-N'-(4-chloro-3-trifluoromethylphenyl)-guanidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.00058
N-(3'-deoxy-beta-D-thymidin-3'-yl)-N'-(4-chloro-3-trifluoromethylphenyl)-isopropylguanidine
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
-
0.268
N-(cyanomethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Homo sapiens
-
IC50: 0.268 mM
0.5
N-(cyanomethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.238
N-(diphenylmethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Homo sapiens
-
IC50: 0.238 mM
0.5
N-(diphenylmethyl)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.038
N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Homo sapiens
-
IC50: 0.038 mM
0.043
N-(diphenylmethyl)-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Human herpesvirus 1
-
IC50: 0.043 mM
0.000046
N-methyl-4-[[[8-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)octyl]oxy](diphenyl)methyl]benzamide
Homo sapiens
-
thymidine kinase 2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml obvine serum albumin, at 37C
0.027
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-2,2-diphenylacetamide
Homo sapiens
-
IC50: 0.027 mM
0.115
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-2,2-diphenylacetamide
Human herpesvirus 1
-
IC50: 0.115 mM
0.028
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-3-(trifluoromethyl)benzamide
Homo sapiens
-
IC50: 0.028 mM
0.072
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-3-(trifluoromethyl)benzamide
Human herpesvirus 1
-
IC50: 0.072 mM
0.033
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-9H-xanthene-9-carboxamide
Homo sapiens
-
IC50: 0.033 mM
0.131
N-[(2Z)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)but-2-en-1-yl]-9H-xanthene-9-carboxamide
Human herpesvirus 1
-
IC50: 0.131 mM
0.287
N-[2-[(diphenylmethyl)amino]-2-oxoethyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Homo sapiens
-
IC50: 0.287 mM
0.5
N-[2-[(diphenylmethyl)amino]-2-oxoethyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Human herpesvirus 1
-
IC50: above 0.5 mM
0.208
N-[cyano(phenyl)methyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Homo sapiens
-
IC50: 0.208 mM
0.495
N-[cyano(phenyl)methyl]-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
Human herpesvirus 1
-
IC50: 0.495 mM
0.075
N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Homo sapiens
-
IC50: 0.075 mM
0.275
N-[cyano(phenyl)methyl]-6-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)hexanamide
Human herpesvirus 1
-
IC50: 0.275 mM
0.5
N3-methyl-1-[(2Z)-4-(trityloxy)but-2-en-1-yl]-thymine
Homo sapiens, Human herpesvirus 1
-
IC50: above 0.5 mM
0.01
penciclovir
Entamoeba histolytica
Q6S4W2
at 37C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.014
Herpes simplex virus
-
-
0.031
-
liver enzyme
0.053
-
-
0.089
Herpes simplex virus
-
-
0.094
-
placenta enzyme
0.124 - 0.183
-
-
0.13
Herpes simplex virus
-
-
0.46
Herpes simplex virus
-
-
7.5
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
activities of recombinant wild-type and mutant enzymes in dimeric or tetrameric form, i.e. in absence or presence of ATP, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 7.2
-
fetal liver
7 - 8.5
-
adult liver
7.4
-
bimodal pH profile with a peak at pH 7.4 and a minor peak at pH 9.0
7.4 - 7.8
-
mitochondrial enzyme
7.5
-
TK-A and TK-C
7.5
-
assay at
7.5
-
assay at
7.5 - 8.5
-
for phosphorylation of thymidine
7.6 - 7.8
-
cytoplasmic enzyme
8
-
assay at
8
exhibits best activity at pH 8.0 at an ATP concentration range between 0.2 and 4.0 mM
8.5
Herpes simplex virus
-
-
9
-
bimodal pH profile with a peak at pH 7.4 and a minor peak at pH 9.0
additional information
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 10.2
-
less than 50% of maximal activity above and below
5 - 8
-
60% of maximal activity at pH 5.0, 50% of maximal activity at pH 8.0
6 - 10
Herpes simplex virus
-
enzyme is active between pH 6 and pH 10
7 - 9.5
-
less than 70% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 37
-
highest activity within this temperature range at 37C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.1
-
linear pH gradient
5.6 - 5.9
-
linear pH gradient in PAGE
6.3 - 6.8
-
variant TK-C
7.6
-
variant TK-B
7.8
-
variant TK-A
9.1
-
linear pH gradient between pH 7 and pH 11
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
healthy and leucemic cells
Manually annotated by BRENDA team
additional information
-
enzyme is expressed during growth, but decreases in amount after starvation
Manually annotated by BRENDA team
additional information
-
expression in nonproliferating tissue
Manually annotated by BRENDA team
additional information
-
TK2 is constitutively expressed along the cell cycle and often is virtually the only thymidine kinase that is physiologically active in non-proliferating and resting cells
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Equine herpesvirus 4 (strain 1942)
Equine herpesvirus 4 (strain 1942)
Equine herpesvirus 4 (strain 1942)
Equine herpesvirus 4 (strain 1942)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Human herpesvirus 1 (strain 17)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)