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Information on EC 2.7.1.158 - inositol-pentakisphosphate 2-kinase and Organism(s) Homo sapiens
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2.7.1.158 inositol-pentakisphosphate 2-kinaseIUBMB Comments
The enzyme can also use Ins(1,4,5,6)P4 and Ins(1,4,5)P3 as substrate. Inositol hexakisphosphate (phytate) accumulates in storage protein bodies during seed development and, when hydrolysed, releases stored nutrients to the developing seedling before the plant is capable of absorbing nutrients from its environment .
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Word Map
- 2.7.1.158
- hexakisphosphate
- phytate
-
multikinase
- 1,2,3,4,5,6-hexakisphosphate
- tetrakisphosphate
-
5/6-kinase
- diagnostics
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
inositol kinase, atipk1, inositol 1,3,4,5,6-pentakisphosphate 2-kinase, inositol pentakisphosphate 2-kinase, gmipk1, ip5 2-k, scipk1, zmipk1a, zmipk1, stitpk1, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
inositol pentakisphosphate 2-kinase
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol 1,3,4,5,6-pentakisphosphate 2-phosphotransferase
The enzyme can also use Ins(1,4,5,6)P4 [2] and Ins(1,4,5)P3 [3] as substrate. Inositol hexakisphosphate (phytate) accumulates in storage protein bodies during seed development and, when hydrolysed, releases stored nutrients to the developing seedling before the plant is capable of absorbing nutrients from its environment [5].
CAS REGISTRY NUMBER
COMMENTARY
134093-01-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme changes its kinase activity towards 1D-myo-inositol hexakisphosphate at a decreasing ATP/ADP ratio to an ADP phosphotransferase activity and dephosphorylate 1D-myo-inositol hexakisphosphate
-
-
?
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
-
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
-
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
preferentially localized in euchromatin and nucleoli, and colocalized with mRNA
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spatial microheterogeneity in the intracellular localization of IP5K in NRK cells, COS7 cell and H1299 overexpressing EGFP-IP5K fusion proteins, also confirmed for the endogenous enzyme
additional information
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the overexpressed IP5K shows locally high concentrations in discrete foci. IP5K is highly concentrated in stress granules
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preferentially localized in euchromatin and nucleoli, and colocalized with mRNA
additional information
IPP2K is present in cell nuclei, where it colocalizes with mRNA in the nucleoli, whereas in the cytoplasm it is found principally in stress granules. Distribution of baseline urine inositol 1,3,4,5,6-pentakisphosphate 2-kinase/creatinine (IPP2K/Cr) in women and men, overview
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additional information
-
IPP2K is present in cell nuclei, where it colocalizes with mRNA in the nucleoli, whereas in the cytoplasm it is found principally in stress granules. Distribution of baseline urine inositol 1,3,4,5,6-pentakisphosphate 2-kinase/creatinine (IPP2K/Cr) in women and men, overview
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
urine inositol pentakisphosphate 2-kinase and changes in kidney structure in early diabetic kidney disease in type 1 diabetes. A higher prevalence of detectable urinary inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPP2K) in type 1 diabetes correlates with early renal function decline. Proximal tubule cells from people with type 1 diabetes and diabetic kidney disease (DKD) express more IPP2K compared with controls. Demographics and clinical characteristics by tertile of baseline urine inositol 1,3,4,5,6-pentakisphosphate 2-kinase/creatinine (IPP2K/Cr), overview
physiological function
association of urine inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPP2K) with the presence and progression of diabetic kidney disease (DKD) lesions. Inositol 6-phosphate is a key intracellular signaling molecule with roles in mRNA editing and chromatin remodeling
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). IPPK_HUMAN
491
0
56017
Swiss-Prot
other Location (Reliability: 2)
B3KVX7_HUMAN
190
0
21682
TrEMBL
other Location (Reliability: 3)
X6R9A6_HUMAN
163
0
18704
TrEMBL
other Location (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
peptide mapping, quantitative LC-selective reaction monitoring-MS (LC-SRM-MS) analysis
additional information
-
peptide mapping, quantitative LC-selective reaction monitoring-MS (LC-SRM-MS) analysis
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21 (Lys) DE3 by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Sf21 cells. Expression of the human enzyme in yeast ipk null strain restores synthesis of 1D-myo-inositol hexakisphosphate and rescues the gle1-2 ipk1-4 lethal phenotype
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recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (Lys) DE3
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
diagnostics
assessing early kidney structural damage in diabetic kidney disease (DKD) via a urine biomarker is an appealing goal, and IPP2K may be a promising candidate
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Verbsky, J.W.; Wilson, M.P.; Kisseleva, M.V.; Majerus, P.W.; Wente, S.R.
The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase
J. Biol. Chem.
277
31857-31862
2002
Homo sapiens
Brehm, M.A.; Schenk, T.M.; Zhou, X.; Fanick, W.; Lin, H.; Windhorst, S.; Nalaskowski, M.M.; Kobras, M.; Shears, S.B.; Mayr, G.W.
Intracellular localization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Biochem. J.
408
335-345
2007
Homo sapiens
Wundenberg, T.; Grabinski, N.; Lin, H.; Mayr, G.W.
Discovery of InsP6-kinases as InsP6-dephosphorylating enzymes provides a new mechanism of cytosolic InsP6 degradation driven by the cellular ATP/ADP ratio
Biochem. J.
462
173-184
2014
Homo sapiens, Rattus norvegicus
Wang, H.; Godage, H.Y.; Riley, A.M.; Weaver, J.D.; Shears, S.B.; Potter, B.V.
Synthetic inositol phosphate analogs reveal that PPIP5K2 has a surface-mounted substrate capture site that is a target for drug discovery
Chem. Biol.
21
689-699
2014
Homo sapiens
Brehm, M.; Wundenberg, T.; Williams, J.; Mayr, G.; Shears, S.
A non-catalytic role for inositol 1,3,4,5,6-pentakisphosphate 2-kinase in the synthesis of ribosomal RNA
J. Cell Sci.
126
437-444
2013
Homo sapiens
-
Looker, H.C.; Merchant, M.L.; Rane, M.J.; Nelson, R.G.; Kimmel, P.L.; Rovin, B.H.; Klein, J.B.; Mauer, M.; Mauer, M.
Urine inositol pentakisphosphate 2-kinase and changes in kidney structure in early diabetic kidney disease in type 1 diabetes
Am. J. Physiol. Renal Physiol.
315
F1484-F1492
2018
Homo sapiens (Q9H8X2), Homo sapiens
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Release 2023.1 (January 2023)
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