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Information on EC 2.7.1.153 - phosphatidylinositol-4,5-bisphosphate 3-kinase and Organism(s) Bos taurus

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IUBMB Comments
This enzyme also catalyses the phosphorylation of PtdIns4P to PtdIns(3,4)P2, and of PtdIns to PtdIns3P. Four mammalian isoforms are known to exist.
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Bos taurus
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
pik3ca, phosphoinositide 3-kinase, pi 3-kinase, pi3-kinase, pi3-k, pi-3k, pik3r1, phosphatidylinositol 3'-kinase, p110alpha, pi 3-k, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
class I phosphoinositide 3-kinase
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class I PI3K
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kinase (phosphorylating), phosphatidylinositol 4,5-diphosphate 3-
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p101-PI3K
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p110delta
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P120-PI3K
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phosphatidylinositol (4,5)-bisphosphate 3-hydroxykinase
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phosphatidylinositol 3-hydroxyl kinase
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PI3Kbeta
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PI3Kgamma
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PtdIns(4,5)P2 3-OH kinase
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PtdIns-3-kinase p101
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PtdIns-3-kinase p110
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PtdInsP 3-OH-kinase
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type I phosphoinositide 3-kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho-group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ATP:1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate 3-phosphotransferase
This enzyme also catalyses the phosphorylation of PtdIns4P to PtdIns(3,4)P2, and of PtdIns to PtdIns3P. Four mammalian isoforms are known to exist.
CAS REGISTRY NUMBER
COMMENTARY hide
103843-30-7
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115926-52-8
cf. EC 2.7.1.137
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
influenza A virus NS1 protein
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influenza A virus infection activates the PI3K/Akt pathway by binding influenza A virus NS1 protein to Val573 of the inter-SH2 domain of the p85beta, but not the p85alpha, regulatory subunit of PI3K. NS1-p85-p110 forms a complex in the cells. The interaction is mediated by the interaction interface between the NS1 SH3 binding motif 1, amino acids 164-167, p85beta Val573, and amino acids 137-142 of NS1, molecular modeling and mechanism, overview. Mutant virus PR8-NS1-141/142 is not able to activate Akt phosphorylation
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PK3CA_BOVIN
1068
0
124328
Swiss-Prot
Secretory Pathway (Reliability: 5)
F1MYM3_BOVIN
1070
0
122810
TrEMBL
Secretory Pathway (Reliability: 2)
A6QPT0_BOVIN
885
0
101907
TrEMBL
other Location (Reliability: 2)
A0A3Q1MD55_BOVIN
968
0
111341
TrEMBL
other Location (Reliability: 2)
E1BE66_BOVIN
1044
0
119904
TrEMBL
other Location (Reliability: 2)
G3MWH3_BOVIN
987
0
113183
TrEMBL
Secretory Pathway (Reliability: 2)
A0A3Q1M2L5_BOVIN
1102
0
126581
TrEMBL
other Location (Reliability: 2)
PDB
SCOP
CATH
UNIPROT
ORGANISM
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
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catalytic subunit p110 and regulatory subunit p85
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
M582V
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site-directed mutagenesis, the full-length p85alpha isoform inter-SH2 domain mutation enables the mutant PI3K to bind influenza A virus NS1 protein leading to activation of the mutant PI3K enzyme activity, molecular modeling, overview
V573M
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site-directed mutagenesis, the p85beta isoform inter-SH2 domain mutation abrogates mutant PI3K binding to influenza A virus NS1 protein, molecular modeling, overview
additional information
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construction of chimeras of 85alpha and p85beta iSH2 domain by overlapping PCR using mouse p85beta and bovine p85alpha as templates
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant tagged proteins by the specific affinity chromatographies
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of His-, FLAG-, or GSt-tagged p85beta in HEK-293T or A-549 cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, Y.; Anderson, D.H.; Liu, Q.; Zhou, Y.
Mechanism of influenza A virus NS1 protein interaction with the p85beta, but not the p85alpha, subunit of phosphatidylinositol 3-kinase (PI3K) and up-regulation of PI3K activity
J. Biol. Chem.
283
23397-23409
2008
Bos taurus, Mus musculus
Manually annotated by BRENDA team