Information on EC 2.7.1.148 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.7.1.148
-
RECOMMENDED NAME
GeneOntology No.
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
Phosphorylation
-, O67060
-
Phosphorylation
A6XAA8, A6XAA9, -
;
Phosphorylation
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
Metabolic pathways
-
methylerythritol phosphate pathway
-
Terpenoid backbone biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
ATP:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol 2-phosphotransferase
The enzyme from Escherichia coli requires Mg2+ or Mn2+. Forms part of an alternative nonmevalonate pathway for terpenoid biosynthesis (for diagram, click here).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
-
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
A6XAA8, A6XAA9
-
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
-
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
-
-
-
-
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
-
-
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
-
-
4-diphosphocytidyl-2-C-methylerythritol 2-kinase
-
-
-
-
4-diphosphocytidyl-2C-methyl-D-erythritol 2-kinase
-
-
-
-
4-diphosphocytidyl-2C-methyl-D-erythritol kinase
O67060
-
4-diphosphocytidyl-2C-methyl-D-erythritol kinase
-
-
AaIspE
O67060
-
CDP-ME
Escherichia coli DH5alpha
-
-
-
CDP-ME
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
-
-
CDP-ME
-
-
CDP-ME kinase
-
-
-
-
CDP-ME kinase
A6XAA8, A6XAA9
-
CDP-ME kinase
-
-
CDP-methylerythritol kinase
-
-
CDPMEK
-
-
-
-
CMK
-
-
-
-
CMK
O67060
-
CMK
A6XAA8, A6XAA9
-
GbCMK1
A6XAA8
Isoenzyme with 433 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%.
GbCMK2
A6XAA9
isoenzyme with 428 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%.
IspDF
-
in Agrobacterium tumefaciens the ispD and ispF genes are fused to encode a bifunctional enzyme that catalyzes the first (synthesis of 4-diphosphocytidyl-2-C-methyl D-erythritol) and third (synthesis of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate) steps of isoprenoid biosynthetic pathway
IspE
O67060
-
Ripening-associated protein pTOM41
-
-
-
-
YchB
A6XAA8, A6XAA9
-
IspE
P62615
gene name
additional information
-
the enzyme belongs to the galactose kinase/homoserine kinase/mevalonate kinase phosphomevalonate kinase superfamily
additional information
-
enzyme belongs to the GHMK super family
CAS REGISTRY NUMBER
COMMENTARY
263016-77-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gene ispE
-
-
Manually annotated by BRENDA team
Escherichia coli DH5alpha
-
-
-
Manually annotated by BRENDA team
strain HB8
-
-
Manually annotated by BRENDA team
Vitis vinifera x Vitis riparia
-
-
-
Manually annotated by BRENDA team
Vitis vinifera x Vitis vinifera
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
enzyme catalyses the fourth reaction step of the 2C-methylerythritol 4-phosphate (MEP) pathway for the biosynthesis of isopentenyl pyrophosphate and its isomer dimethylallyl pyrophosphate
physiological function
-
the MEP pathway is essential in the malaria parasite Plasmodium falciparum and in most eubacteria, including the causal agents for diverse and serious human diseases like leprosy, bacterial meningitis, various gastrointestinal and sexually transmitted infections, tuberculosis, and certain types of pneumonia
physiological function
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
-
involved in terpenoid metabolism
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
light
A6XAA8, A6XAA9, -
Selective increase in the transcript abundance of GbCMK2 in the radicles by induction of light.
-
methyl jasmonate
A6XAA8, A6XAA9, -
Selective increase in the transcript abundance of GbCMK2 in adicles.
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.2
-
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
pH not specified in the publication, 22°C
0.121
-
4-diphosphocytidyl-2C-methyl-D-erythritol
-, O67060
-
0.02
-
ATP
-
pH not specified in the publication, 22°C
0.222
-
ATP
-, O67060
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0042
-
ethyl [4-amino-2-oxo-5-(3-[[(2,2,2-trifluoroethyl)sulfonyl]amino]prop-1-en-1-yl)pyrimidin-1(2H)-yl]acetate
-
competitive inhibition constant
0.0037
-
N-[3-(4-amino-1-benzyl-2-oxo-1,2-dihydropyrimidin-5-yl)prop-2-en-1-yl]ethanesulfonamide
-
competitive inhibition constant
0.0016
-
N-[3-[4-amino-2-oxo-1-(1H-pyrazol-5-ylmethyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]ethanesulfonamide
-
competitive inhibition constant
0.0012
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]-1,1,1-trifluoromethanesulfonamide
-
competitive inhibition constant
0.00036
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]-2,2,2-trifluoroethanesulfonamide
-
competitive inhibition constant
0.0163
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]benzenesulfonamide
-
competitive inhibition constant
0.00029
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]cyclopropanesulfonamide
-
competitive inhibition constant
0.00064
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]ethanesulfonamide
-
competitive inhibition constant
0.0026
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]methanesulfonamide
-
competitive inhibition constant
0.0082
-
N-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]propane-1-sulfonamide
-
competitive inhibition constant
0.0323
-
N-[3-[4-amino-2-oxo-1-(tetrahydrofuran-2-ylmethyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]-2,2,2-trifluoroethanesulfonamide
-
competitive inhibition constant
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.1
-
A6XAA8, A6XAA9, -
theoretical value, predicted
5.2
-
A6XAA8, A6XAA9, -
theoretical value, predicted
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-, O67060
cloned in Escherichia coli
Manually annotated by BRENDA team
A6XAA8, A6XAA9, -
5 embryos are pooled to prepare a RNA sample, used for complementation assay; 5 embryos are pooled to prepare a RNA sample, used for complementation assay
Manually annotated by BRENDA team
A6XAA8, A6XAA9, -
5 embryos are pooled to prepare a RNA sample, used for complementation assay; 5 embryos are pooled to prepare a RNA sample, used for complementation assay
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
A6XAA8, A6XAA9, -
isoenzyme contains chloroplast transit peptide sequence; isoenzyme contains chloroplast transit peptide sequence
Manually annotated by BRENDA team
A6XAA8, A6XAA9, -
-
Manually annotated by BRENDA team
A6XAA8, A6XAA9, -
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Escherichia coli (strain K12)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196)
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196)
Mycobacterium abscessus (strain ATCC 19977 / DSM 44196)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
homodimer
-
determined by gel filtration, asymmetric unit consists of 2 subunits A and B which assemble with C2 symmetry to form an extended homodimer, but only 4% of the total surface area of the 2 monomers is involved in dimer formation
monomer
-, O67060
Gel-filtration and analytical ultracentrifugation indicate that AaIspE is a monomer in solution
additional information
-
the enzyme subunit displays the alpha/beta fold characteristic of the galactose kinase/homoserine kinase/mevalonate kinase phosphomevalonate kinase superfamily, arranged into cofactor and substrate-binding domains with the catalytic center positioned in a deep cleft between domains, quarternary structure, overview