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Information on EC 2.7.1.11 - 6-phosphofructokinase and Organism(s) Bos taurus

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EC Tree
IUBMB Comments
The enzyme from rabbit muscle displays absolute stereoselectivity for the beta-anomer of D-fructofuranose 6-phosphate [9-11]. D-Tagatose 6-phosphate and sedoheptulose 7-phosphate can act as acceptors. UTP, CTP and ITP can act as donors. Not identical with EC 2.7.1.105 6-phosphofructo-2-kinase.
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Bos taurus
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The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
phosphofructokinase, 6-phosphofructokinase, 6-phosphofructo-1-kinase, pfk-1, phosphofructokinase-1, pfk-m, phosphofructokinase 1, atp-dependent phosphofructokinase, atp-pfk, pfk-l, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phosphofructokinase, platelet type
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-
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6-phosphofructose 1-kinase
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-
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6-phosphofructose-1-kinase
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-
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ATP-dependent phosphofructokinase
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ATP-PFK
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D-fructose-6-phosphate 1-phosphotransferase
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fructose 6-phosphate kinase
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fructose 6-phosphokinase
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kinase, phosphofructo- (phosphorylating)
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nucleotide triphosphate-dependent phosphofructokinase
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PFK
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-
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PFK-1
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PFK1
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-
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PFK2
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-
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phospho-1,6-fructokinase
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-
-
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phosphofructokinase
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phosphofructokinase 1
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phosphofructokinase-1
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phosphohexokinase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-fructose-6-phosphate 1-phosphotransferase
The enzyme from rabbit muscle displays absolute stereoselectivity for the beta-anomer of D-fructofuranose 6-phosphate [9-11]. D-Tagatose 6-phosphate and sedoheptulose 7-phosphate can act as acceptors. UTP, CTP and ITP can act as donors. Not identical with EC 2.7.1.105 6-phosphofructo-2-kinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-80-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose 6-phosphate
ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose 6-phosphate
ADP + D-fructose 1,6-bisphosphate
show the reaction diagram
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-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
-
Mn2+
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activation
additional information
-
Na+ has little or no effect at 140 nM PFK-1
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
citrate
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strong inhibition
D-Fructose 1-phosphate
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K+
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about 30% residual activity of 30 nM PFK-1 in the presence of 0.2 M K+
Lactate dehydrogenase
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lactate dehydrogenase suspended in 3.2 M ammonium sulfate inhibits 30 nM PFK-1 resulting in a more than 50% inhibition of activity
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Na+
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about 70% residual activity of 30 nM PFK-1 in the presence of 0.2 M Na+
NH4+
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about 40% residual activity of 30 nM PFK-1 in the presence of 0.2 M NH4+
additional information
-
not inhibited by fructose 1,6-bisphosphate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose 1,6-bisphosphate
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activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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kinetic data of various organism
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67.5
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93
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heart muscle PFK
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
calf
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PFKAL_BOVIN
780
0
85292
Swiss-Prot
other Location (Reliability: 2)
PFKAM_BOVIN
779
0
85294
Swiss-Prot
other Location (Reliability: 2)
A0A3Q1M6K7_BOVIN
850
0
93427
TrEMBL
Mitochondrion (Reliability: 4)
A0A3Q1MNP9_BOVIN
778
0
85452
TrEMBL
other Location (Reliability: 2)
A0A3Q1NJ70_BOVIN
909
0
97498
TrEMBL
other Location (Reliability: 3)
E1BCW3_BOVIN
960
0
102968
TrEMBL
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
400000
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gel filtration
86000
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1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis
88000
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1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis
92000
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1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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1 * 92000 + 1 * 88000 + 2 * 86000, SDS-PAGE and densitometric analysis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cibacron Blue F3GA affinity chromatography, ion-exchange, gel filtration
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bloxham, D.P.; Lardy, H.A.
Phosphofructokinase
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
8
239-278
1973
Klebsiella aerogenes, Arthrobacter crystallopoietes, Glutamicibacter nicotianae, Bos taurus, Brassica oleracea var. gemmifera, Saccharomyces cerevisiae, Gallus gallus, Clostridium pasteurianum, Oryctolagus cuniculus, Daucus carota, Dictyostelium discoideum, Escherichia coli, Fasciola hepatica, Thermus thermophilus, Ovis aries, Homo sapiens, Lactiplantibacillus plantarum, Lacticaseibacillus casei, Mus musculus, Neurospora crassa, Pisum sativum, Rattus norvegicus, Zea mays
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Manually annotated by BRENDA team
Fukushima, E.; Sugiya, H.
Purification and characterization of phosphofructokinase in bovine parotid gland
Int. J. Biochem.
24
1307-1314
1992
Bos taurus
Manually annotated by BRENDA team
Massey, T.; Deal, W.C.
Phosphofructokinase from porcine liver and kidney and from other mammalian tissues
Methods Enzymol.
42C
99-110
1975
Bos taurus, Capra hircus, Gallus gallus, Oryctolagus cuniculus, Ovis aries, Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Russell, P.; Williams, A.; Marquez, K.; Hua, T.; Ehya, F.; Hardamon, C.; Tallman, T.; Valdez, P.
Effect of ammonium, sodium, and potassium ions on rabbit muscle phosphofructokinase-1 and adenylate kinase activities
J. Enzyme Inhib. Med. Chem.
24
930-936
2009
Bos taurus, Oryctolagus cuniculus
Manually annotated by BRENDA team