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Information on EC 2.7.1.107 - diacylglycerol kinase (ATP) and Organism(s) Dictyostelium discoideum

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EC Tree
IUBMB Comments
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
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This record set is specific for:
Dictyostelium discoideum
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Word Map
The taxonomic range for the selected organisms is: Dictyostelium discoideum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dgk, diacylglycerol kinase, dag kinase, dg kinase, dgkzeta, dagk, dgkalpha, dgk-zeta, diglyceride kinase, dgkepsilon, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,2-diacylglycerol kinase
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adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase
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arachidonoyl-specific diacylglycerol kinase
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ATP:diacylglycerol phosphotransferase
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DAGK
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DAGKalpha
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DG kinase
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DGK
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DGK-alpha
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DGK-theta
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DGKbeta
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DGKdelta
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DGKgamma
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DGKiota
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DGKksi
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diacylglycerol kinase
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diacylglycerol kinase (ATP dependent)
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diacylglycerol:ATP kinase
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diglyceride kinase
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kinase (phosphorylating), 1,2-diacylglycerol
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kinase, 1,2-diacylglycerol (phosphorylating)
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sn-1,2-diacylglycerol kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
catalytic and regulatory mechanisms
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:1,2-diacyl-sn-glycerol 3-phosphotransferase
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
CAS REGISTRY NUMBER
COMMENTARY hide
60382-71-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
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?
ATP + 1,2-dihexanoyl-sn-glycerol
ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate
show the reaction diagram
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?
ATP + 1,2-dioctanoyl-sn-glycerol
ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
show the reaction diagram
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preferred substrate
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?
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphatidic acid
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highly stimulating
phosphatidylinositol 4,5-bisphosphate
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highly stimulating
phosphatidylserine
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strong activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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kinetics, follow a Michaelis-Menten mechanism in case of medium-chain diacylglycerols, and are strongly dependent on detergent concentration and sort used in the micelles in the assay in case of longer-chain substrates showing sigmoidal kinetics, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DGKA_DICDI
887
0
99177
Swiss-Prot
other Location (Reliability: 3)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme, by ultracentrifugation, gel filtration, and antibody affinity chromatography
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ostroski, M.; Tu-Sekine, B.; Raben, D.M.
Analysis of a novel diacylglycerol kinase from Dictyostelium discoideum: DGKA
Biochemistry
44
10199-10207
2005
Dictyostelium discoideum, Dictyostelium discoideum HL5
Manually annotated by BRENDA team