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Information on EC 2.7.1.107 - diacylglycerol kinase (ATP) and Organism(s) Bos taurus

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EC Tree
IUBMB Comments
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
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This record set is specific for:
Bos taurus
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dgk, diacylglycerol kinase, dag kinase, dg kinase, dgkzeta, dagk, dgkalpha, dgk-zeta, diglyceride kinase, dgkepsilon, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,2-diacylglycerol kinase
-
-
-
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1,2-diacylglycerol kinase epsilon
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adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase
-
-
-
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arachidonoyl-specific diacylglycerol kinase
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-
-
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ATP:diacylglycerol phosphotransferase
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-
-
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DAGKalpha
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-
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DAGKepsilon
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DG kinase
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-
-
-
DGK
-
-
-
-
DGK-alpha
-
-
-
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DGK-theta
-
-
-
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DGKbeta
-
-
-
-
DGKdelta
-
-
-
-
DGKgamma
-
-
-
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DGKiota
-
-
-
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DGKksi
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-
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diacylglycerol kinase
-
-
-
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diacylglycerol kinase (ATP dependent)
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-
-
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diacylglycerol kinase epsilon
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-
diacylglycerol:ATP kinase
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-
-
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diglyceride kinase
-
-
-
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kinase (phosphorylating), 1,2-diacylglycerol
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-
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-
kinase, 1,2-diacylglycerol (phosphorylating)
-
-
-
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sn-1,2-diacylglycerol kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1,2-diacyl-sn-glycerol 3-phosphotransferase
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
CAS REGISTRY NUMBER
COMMENTARY hide
60382-71-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1,2-dioleoylglycerol
ADP + 1,2-dioleoylglycerol 3-phospate
show the reaction diagram
-
low activity
-
-
?
ATP + 1-stearoyl-2-arachidonoylglycerol
ADP + 1-stearoyl-2-arachidonoylglycerol 3-phosphate
show the reaction diagram
-
preferred enzyme, 6fold higher activity compared to substrate 1,2-dioleoylglycerol
-
-
?
additional information
?
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enzyme assays with isolated rod outer segments prepared under room light from retinas obtained from dark-adapted bovine eyes
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no inhibition of activity of isozyme DAGKepsilon with 1-stearoyl-2-arachidonoylglycerol by R59022, a type I DAGK inhibitor
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the selective distribution of isozyme DAGKepsilon in photoreceptor cells is a light-dependent mechanism that promotes increased 1-stearoyl, 2-arachidonoylglycerol removal and synthesis of 1-stearoyl, 2-arachidonoyl phosphatidic acid in the sensorial portion of this cell
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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in bovine rod outer segments obtained from retinas under room light conditions, DAGKepsilon and type I DAGK activity is present. Isozyme DAGKepsilon appears to be higher in rod outer segments from bleached bovine retinas than in those under dark conditions
Manually annotated by BRENDA team
additional information
-
light-dependent outer segment localization of DAGKepsilon, DAGKepsilon distribution in the photoreceptor cell is dependent on PIP2-PLC activation by light
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
in bovine rod outer segments obtained from retinas under room light conditions, DAGKepsilon and type I DAGK activity is present. Isozyme DAGKepsilon appears to be higher in rod outer segments from bleached bovine retinas than in those under dark conditions
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme is involved in a mechanism of light-regulated DAGK activity in the photoreceptors of the vertebrate species. Protein kinase C-dependent phosphorylation of retina rod outer segments (obtained from bovine retinas at room light) regulates DAGK activity
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DGKA_BOVIN
734
0
82672
Swiss-Prot
other Location (Reliability: 2)
E1B9E4_BOVIN
791
0
88804
TrEMBL
other Location (Reliability: 1)
A0A3Q1LYT4_BOVIN
799
0
89685
TrEMBL
other Location (Reliability: 1)
F1MI58_BOVIN
509
0
56405
TrEMBL
other Location (Reliability: 2)
A5PK18_BOVIN
420
0
46647
TrEMBL
Secretory Pathway (Reliability: 5)
A6QPL5_BOVIN
1215
0
133974
TrEMBL
other Location (Reliability: 2)
A0A3Q1LLJ7_BOVIN
987
0
109877
TrEMBL
other Location (Reliability: 1)
A0A3Q1NMG0_BOVIN
734
0
82703
TrEMBL
other Location (Reliability: 1)
A0A3Q1M892_BOVIN
524
1
59285
TrEMBL
Secretory Pathway (Reliability: 3)
A0A3Q1N531_BOVIN
862
0
96411
TrEMBL
other Location (Reliability: 3)
A0A3Q1M6H5_BOVIN
773
0
87372
TrEMBL
other Location (Reliability: 2)
G5E5G8_BOVIN
1145
0
126458
TrEMBL
other Location (Reliability: 4)
A0A3Q1NJA6_BOVIN
1234
1
136602
TrEMBL
other Location (Reliability: 4)
A0A3Q1N653_BOVIN
928
0
103996
TrEMBL
other Location (Reliability: 2)
A0A3Q1LN52_BOVIN
708
0
79653
TrEMBL
other Location (Reliability: 2)
A0A3Q1NNM0_BOVIN
766
0
86532
TrEMBL
other Location (Reliability: 1)
F6Q508_BOVIN
419
0
46514
TrEMBL
Secretory Pathway (Reliability: 5)
E1B7K6_BOVIN
564
1
63604
TrEMBL
Secretory Pathway (Reliability: 3)
A0A3Q1LPG9_BOVIN
540
1
60815
TrEMBL
Secretory Pathway (Reliability: 3)
A0A3Q1LRI9_BOVIN
761
0
86203
TrEMBL
other Location (Reliability: 2)
A0A3Q1M4U6_BOVIN
1142
0
126256
TrEMBL
other Location (Reliability: 2)
F1MCG9_BOVIN
1226
0
137365
TrEMBL
other Location (Reliability: 2)
E1B7U7_BOVIN
803
0
90536
TrEMBL
other Location (Reliability: 2)
F1N2W5_BOVIN
1002
0
111550
TrEMBL
other Location (Reliability: 1)
E1BGA9_BOVIN
942
0
101668
TrEMBL
other Location (Reliability: 2)
A0A3Q1N4W0_BOVIN
1044
0
116256
TrEMBL
other Location (Reliability: 1)
A0A3Q1MFM9_BOVIN
421
0
46946
TrEMBL
other Location (Reliability: 5)
A6QR07_BOVIN
420
0
46746
TrEMBL
Secretory Pathway (Reliability: 5)
A0A3Q1LSM5_BOVIN
752
0
84253
TrEMBL
other Location (Reliability: 1)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Natalini, P.M.; Zulian, S.E.; Ilincheta de Boschero, M.G.; Giusto, N.M.
Diacylglycerol kinase epsilon in bovine and rat photoreceptor cells. Light-dependent distribution in photoreceptor cells
Exp. Eye Res.
112
139-150
2013
Bos taurus, Rattus norvegicus, Rattus norvegicus Wistar
Manually annotated by BRENDA team