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EC Tree
IUBMB Comments Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
The taxonomic range for the selected organisms is: Arabidopsis thaliana The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dgk, diacylglycerol kinase, dag kinase, dg kinase, dgkzeta, dagk, dgkalpha, dgk-zeta, diglyceride kinase, dgkepsilon,
more
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1,2-diacylglycerol kinase
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adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase
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arachidonoyl-specific diacylglycerol kinase
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ATP:diacylglycerol phosphotransferase
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diacylglycerol kinase
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diacylglycerol kinase (ATP dependent)
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diacylglycerol kinase 4
isoform
diacylglycerol:ATP kinase
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diglyceride kinase
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kinase (phosphorylating), 1,2-diacylglycerol
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kinase, 1,2-diacylglycerol (phosphorylating)
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sn-1,2-diacylglycerol kinase
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DGK
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DGK2
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isozyme
DGK4
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isozyme
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phospho group transfer
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ATP:1,2-diacyl-sn-glycerol 3-phosphotransferase
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
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ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
ATP + 1-NBD-decanoyl-2-decanoyl-sn-glycerol
ADP + 1-NBD-decanoyl-2-decanoyl-sn-glycerol 3-phosphate
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?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
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?
additional information
?
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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i.e. phosphatidic acid
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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i.e. phosphatidic acid
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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1,2-diacyl-sn-glycerol 3-phosphate is phosphatidic acid
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ir
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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the enzyme binds and regulates signalling proteins which are activated by either diacylglycerol or phosphatidic acid
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ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
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ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
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additional information
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complex enzyme regulation, overview, the enzyme is involved in several processes such as cell growth, neuronal transmission, and cytoskeleton remodeling
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additional information
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the isozyme DGK2 is involved in cold signal transduction
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additional information
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the isozyme DGK2 is involved in cold signal transduction
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additional information
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isoform DGK4 has also guanylyl cyclase activity
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ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
additional information
?
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
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the enzyme binds and regulates signalling proteins which are activated by either diacylglycerol or phosphatidic acid
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additional information
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complex enzyme regulation, overview, the enzyme is involved in several processes such as cell growth, neuronal transmission, and cytoskeleton remodeling
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additional information
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the isozyme DGK2 is involved in cold signal transduction
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additional information
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the isozyme DGK2 is involved in cold signal transduction
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ATP
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Mg2+
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Mg2+
5 mM used in assay conditions
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LiCl
more than 50% inhibition at above 200 mM
NaCl
more than 50% inhibition at above 200 mM
R59022
specific inhibitor
sodium deoxycholate
at 5 mM
Triton X-100
strong inhibition at up to 2%
additional information
isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+
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additional information
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isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+
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additional information
the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C
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additional information
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the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C
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0.125
1,2-dioleoyl-sn-glycerol
pH 7.5, 25°C
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additional information
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additional information
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6.5 - 7.8
rapid decrease below pH 6.5 and above pH 7.8
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brenda
2 isozymes DGK1 and DGK2
SwissProt
brenda
ecotype Col-0
UniProt
brenda
ecotype Col-0
SwissProt
brenda
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high expression
brenda
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high expression
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especially cauline
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high expression
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additional information
isozyme DGK2 expression in various tissues, expression pattern
brenda
additional information
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isozyme DGK2 expression in various tissues, expression pattern
brenda
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brenda
additional information
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subcellular localization of isozymes, overview, the enzyme must undergo membrane translocation for access of diacylglycerols
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brenda
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brenda
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fusion constructs of Green Fluorescent Protein to truncated forms of diacylglycerol kinase 1 and diacylglycerol kinase 2 missing the catalytic and accessory domains
brenda
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malfunction
disruption of isoform DGK4 does not impair male gametophytic development but reduces in vivo performance and alters pollen tube Youngs modulus and adhesion properties
malfunction
isoform dgk2-deficient plants are gametophyte lethal, although parental single homozygous plants are viable. The dgk2/dgk4 double heterozygote shows defective pollen tube growth and seed development because of nonviable mutant gametes
malfunction
isoform dgk4-deficient plants are gametophyte lethal, although parental single homozygous plants are viable. The dgk2/dgk4 double heterozygote shows defective pollen tube growth and seed development because of nonviable mutant gametes
physiological function
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DGKs broadly regulate signaling events by virtue of their ability to provide 1,2-diacyl-sn-glycerol 3-phosphate (phosphatidic acid) for the synthesis of phosphatidylinositols
physiological function
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in response to cold temperatures, there is a very fast accumulation of phosphatidic acid in Arabidopsis seedlings and leaf discs. Radiolabeling studies indicate a dominant role of diacylglycerol kinase under these conditions
physiological function
enzyme-deficient pollen tubes exhibit altered membrane recycling and phospholipid levels
physiological function
isoform DGK4 is crucial for gametogenesis and biosynthesis of phosphatidylglycerol and phosphatidylinositol in the endoplasmic reticulum
physiological function
isoforms DGK2 is crucial for gametogenesis and biosynthesis of phosphatidylglycerol and phosphatidylinositol in the endoplasmic reticulum
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DGK2_ARATH
712
1
79405
Swiss-Prot
Secretory Pathway (Reliability: 5 )
DGK3_ARATH
488
0
53881
Swiss-Prot
other Location (Reliability: 3 )
DGK4_ARATH
487
0
54181
Swiss-Prot
Chloroplast (Reliability: 4 )
DGK5_ARATH
509
0
57377
Swiss-Prot
other Location (Reliability: 4 )
DGK6_ARATH
466
0
52490
Swiss-Prot
other Location (Reliability: 2 )
DGK7_ARATH
492
0
54572
Swiss-Prot
other Location (Reliability: 3 )
DGK1_ARATH
728
1
79984
Swiss-Prot
other Location (Reliability: 1 )
A0A1P8BCS4_ARATH
687
1
75436
TrEMBL
other Location (Reliability: 1 )
A0A654EUM8_ARATH
491
0
55202
TrEMBL
other Location (Reliability: 4 )
A0A178VP38_ARATH
491
0
55111
TrEMBL
other Location (Reliability: 4 )
A0A178UTP0_ARATH
426
0
47877
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A7G2F795_ARATH
557
0
62077
TrEMBL
other Location (Reliability: 3 )
A0A5S9YF64_ARATH
487
0
54181
TrEMBL
Chloroplast (Reliability: 4 )
A0A178UJM6_ARATH
712
1
79453
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A178VS35_ARATH
491
0
55168
TrEMBL
other Location (Reliability: 4 )
A0A654FZ89_ARATH
728
0
79940
TrEMBL
other Location (Reliability: 1 )
A0A178ULH8_ARATH
728
1
79984
TrEMBL
other Location (Reliability: 1 )
A0A5S9YH93_ARATH
712
1
79360
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A654GEA6_ARATH
712
1
79405
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A654GC23_ARATH
486
0
54047
TrEMBL
Chloroplast (Reliability: 4 )
A0A178UUX1_ARATH
492
0
54572
TrEMBL
other Location (Reliability: 3 )
A0A7G2E7U3_ARATH
537
0
60328
TrEMBL
other Location (Reliability: 4 )
A0A7G2FHU2_ARATH
474
0
52836
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A654EZ33_ARATH
488
0
53891
TrEMBL
other Location (Reliability: 3 )
A0A5S9XWK7_ARATH
457
0
51485
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A178VZY0_ARATH
488
0
53881
TrEMBL
other Location (Reliability: 3 )
A0A178UMM1_ARATH
487
0
54165
TrEMBL
Chloroplast (Reliability: 4 )
A0A7G2F337_ARATH
365
0
41068
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A1P8B509_ARATH
374
0
41192
TrEMBL
other Location (Reliability: 3 )
A0A5S9X032_ARATH
515
0
58013
TrEMBL
other Location (Reliability: 4 )
A0A5S9WZE9_ARATH
488
0
53851
TrEMBL
other Location (Reliability: 3 )
A0A178UVY3_ARATH
347
0
38836
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A654FTK0_ARATH
445
0
50147
TrEMBL
other Location (Reliability: 2 )
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140000
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
79400
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
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?
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
additional information
structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview
additional information
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structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview
additional information
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structure-function relationships of isozyme domain motifs, overview
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additional information
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expression of fusion constructs of Green Fluorescent Protein to truncated forms of diacylglycerol kinase 1 and diacylglycerol kinase 2 missing the catalytic and accessory domains. Fusion proteins are localized to the endoplasmic reticulum. Fusion constructs of N-terminal 50 amino acid residues of diacylglycerol kinase 1 and the 43 residues of diacylglycerol kinase 2 to the Yellow Fluorescent Protein alos localize to the endoplasmic reticulum
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CHAPS is the most effective detergent for isozyme DGK2 solubilization, NusA/His6-tagged fusion isozyme DGK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Ni-NTA column chromatography and Sephadex G-25M gel filtration
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AtDGK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as NusA/His6-tagged isozyme DGK2 in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
expression in Arabidopsis thaliana protoplasts
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Luo, B.; Regier, D.S.; Prescott, S.M.; Topham, M.K.
Diacylglycerol kinases
Cell. Signal.
16
983-989
2004
Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mammalia, no activity in Saccharomyces cerevisiae
brenda
Gomez-Merino, F.C.; Brearley, C.A.; Ornatowska, M.; Abdel-Haliem, M.E.; Zanor, M.I.; Mueller-Roeber, B.
AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression
J. Biol. Chem.
279
8230-8241
2004
Arabidopsis thaliana (Q9FFN7), Arabidopsis thaliana
brenda
Vaultier, M.N.; Cantrel, C.; Guerbette, F.; Boutte, Y.; Vergnolle, C.; Cicek, D.; Bolte, S.; Zachowski, A.; Ruelland, E.
The hydrophobic segment of Arabidopsis thaliana cluster I diacylglycerol kinases is sufficient to target the proteins to cell membranes
FEBS Lett.
582
1743-1748
2008
Arabidopsis thaliana
brenda
Arisz, S.A.; Testerink, C.; Munnik, T.
Plant PA signaling via diacylglycerol kinase
Biochim. Biophys. Acta
1791
869-875
2009
Arabidopsis thaliana
brenda
Cai, J.; Abramovici, H.; Gee, S.H.; Topham, M.K.
Diacylglycerol kinases as sources of phosphatidic acid
Biochim. Biophys. Acta
1791
942-948
2009
Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Sus scrofa, Mus musculus (Q80UP3)
brenda
Arisz, S.A.; van Wijk, R.; Roels, W.; Zhu, J.K.; Haring, M.A.; Munnik, T.
Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase
Front. Plant Sci.
4
01
2013
Arabidopsis thaliana
brenda
Vaz Dias, F.; Serrazina, S.; Vitorino, M.; Marchese, D.; Heilmann, I.; Godinho, M.; Rodrigues, M.; Malho, R.
A role for diacylglycerol kinase 4 in signalling crosstalk during Arabidopsis pollen tube growth
New Phytol.
222
1434-1446
2019
Arabidopsis thaliana (Q1PDI2)
brenda
Angkawijaya, A.; Nguyen, V.; Gunawan, F.; Nakamura, Y.
A pair of Arabidopsis diacylglycerol kinases essential for gametogenesis and endoplasmic reticulum phospholipid metabolism in leaves and flowers
Plant Cell
32
2602-2620
2020
Arabidopsis thaliana (Q1PDI2), Arabidopsis thaliana (Q9FFN7)
brenda