Information on EC 2.6.1.70 - aspartate-phenylpyruvate transaminase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
2.6.1.70
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RECOMMENDED NAME
GeneOntology No.
aspartate-phenylpyruvate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-aspartate + phenylpyruvate = oxaloacetate + L-phenylalanine
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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SYSTEMATIC NAME
IUBMB Comments
L-aspartate:phenylpyruvate aminotransferase
The enzyme from Pseudomonas putida also acts on 4-hydroxy-phenylpyruvate and, more slowly, on L-glutamate and L-histidine.
CAS REGISTRY NUMBER
COMMENTARY hide
99533-45-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + L-aspartate
L-glutamate + oxaloaspartate
show the reaction diagram
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34% activity with respect to L-phenylpyruvate
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-
?
2-oxoglutarate + L-phenylalanine
L-glutamate + 3-phenylpyruvate
show the reaction diagram
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reverse reaction, 31% activity with respect to oxalacetate
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r
2-oxoisovalerate + L-aspartate
L-norvaline + oxaloacetate
show the reaction diagram
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6% activity with respect to L-phenylpyruvate
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-
?
2-oxoisovalerate + L-phenylalanine
L-norvaline + 3-phenylpyruvate
show the reaction diagram
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reverse reaction, 8% activity with respect to oxalacetate
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-
r
3-phenylpyruvate + L-histidine
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-phenylalanine
show the reaction diagram
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4% activity with respect to L-aspartate
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?
3-phenylpyruvate + L-tyrosine
p-hydroxyphenylpyruvate + L-phenylalanine
show the reaction diagram
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27% activity with respect to aspartate
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-
?
beta-methyl-DL-aspartate + 3-phenylpyruvate
3-methyl-2-oxobutanedioate + L-phenylalanine
show the reaction diagram
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2% activity with respect to L-aspartate
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-
?
DL-p-fluorophenylalanine + oxaloacetate
p-fluorophenylpyruvate + L-aspartate
show the reaction diagram
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reverse reaction, 51% activity with respect to L-phenylalanine
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r
indolepyruvate + L-aspartate
oxaloaspartate + L-tryptophan
show the reaction diagram
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10% activity with respect to L-phenylpyruvate
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-
?
L-aspartate + 3-phenylpyruvate
oxaloacetate + L-phenylalanine
show the reaction diagram
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oxaloacetate decomposition drives the reaction towards L-phenylalanine
r
L-tryptophan + 3-phenylpyruvate
3-indole-2-oxopropanoate + L-phenylalanine
show the reaction diagram
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40% activity with respect to aspartate
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?
L-tryptophan + oxaloacetate
3-indole-2-oxopropanoate + L-aspartate
show the reaction diagram
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reverse reaction, 77% activity with respect to L-phenylalanine
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r
oxaloacetate + L-histidine
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-aspartate
show the reaction diagram
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reverse reaction, 4% activity with respect to L-phenylalanine
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r
oxaloacetate + L-tyrosine
p-hydroxyphenylpyruvate + L-aspartate
show the reaction diagram
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reverse reaction, 38% activity with respect to L-phenylalanine
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r
p-hydroxyphenylpyruvate + L-aspartate
oxaloacetate + L-tyrosine
show the reaction diagram
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54% activity with respect to L-phenylpyruvate
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?
p-hydroxyphenylpyruvate + L-phenylalanine
L-tyrosine + 3-phenylpyruvate
show the reaction diagram
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reverse reaction, 52% activity with respect to oxalacetate
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-aspartate + 3-phenylpyruvate
oxaloacetate + L-phenylalanine
show the reaction diagram
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oxaloacetate decomposition drives the reaction towards L-phenylalanine
r
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CoCl2
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retains 35% activity at 10 mM
EDTA
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complete inhibition at 10 mM
HgCl2
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irreversible and complete inhibition is observed at concentrations about 0.1 mM
KCN
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irreversible inhibitor
MnCl2
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retains 65% activity at 10 mM
p-chloromercuribenzoate
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irreversible and complete inhibition is observed at concentrations about 0.1 mM
phenyl 2-oxopropanoate
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phenylhydrazine
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irreversible inhibitor; retains 56% activity at 0.01 mM, retains 18% activity at 0.1 mM
pyridoxal 5'-phosphate
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at concentrations higher than 0.05 mM inhibitory effects are observed
Semicarbazide
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irreversible inhibitor; retains 59% activity at 0.01 mM, retains 24% activity at 0.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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the addition to the reaction mixture immediately before carrying out the enzymatic assay has no influence on activity, but preincubation increases the activity about twofold; the optimum of activation is obtained with a 0.05 mM concentration
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
55
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maximum reaction velocity is observed
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 36000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, 50% glycerol without loss of activity for over two years
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
centrifugation, DEAE-Sephacel and Sephacryl S-200
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centrifugation, streptomycin sulfate precipitation, several chromatographic steps and disc-gel electrophoresis
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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L-phenylalanine production as a building block of aspartame