Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.6.1.1 - aspartate transaminase and Organism(s) Mus musculus

for references in articles please use BRENDA:EC2.6.1.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.1 aspartate transaminase
IUBMB Comments
A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis , some EC 2.6.1.57 activity can be found in this enzyme from other sources ; indeed the enzymes are identical in Trichomonas vaginalis .
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
aspartate transaminase, asat, glutamic oxaloacetic transaminase, glutamate oxaloacetate transaminase, glutamic-oxaloacetic transaminase, asp at, aspat, aspartate at, glutamic-oxalacetic transaminase, aat-2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-oxoglutarate-glutamate aminotransferase
-
-
-
-
aminotransferase, aspartate
-
-
-
-
aspartate alpha-ketoglutarate transaminase
-
-
-
-
aspartate aminotransferase
aspartate-2-oxoglutarate transaminase
-
-
-
-
aspartate:2-oxoglutarate aminotransferase
-
-
-
-
aspartic acid aminotransferase
-
-
-
-
aspartic aminotransferase
-
-
-
-
aspartyl aminotransferase
-
-
-
-
AspT
-
-
-
-
C-S lyase
-
-
cAST
-
-
glutamate oxaloacetate transaminase
-
-
-
-
glutamate-oxalacetate aminotransferase
-
-
-
-
glutamate-oxalate transaminase
-
-
-
-
glutamic oxalic transaminase
-
-
-
-
glutamic-aspartic aminotransferase
-
-
-
-
glutamic-aspartic transaminase
-
-
-
-
glutamic-oxalacetic transaminase
-
-
-
-
glutamic-oxaloacetic transaminase
-
-
-
-
GOT (enzyme)
-
-
-
-
L-aspartate transaminase
-
-
-
-
L-aspartate-2-ketoglutarate aminotransferase
-
-
-
-
L-aspartate-2-oxoglutarate aminotransferase
-
-
-
-
L-aspartate-2-oxoglutarate-transaminase
-
-
-
-
L-aspartate-alpha-ketoglutarate transaminase
-
-
-
-
L-aspartic aminotransferase
-
-
-
-
mitAAT
-
-
oxaloacetate transferase
-
-
-
-
oxaloacetate-aspartate aminotransferase
-
-
-
-
transaminase A
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-aspartate:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis [7], some EC 2.6.1.57 activity can be found in this enzyme from other sources [8]; indeed the enzymes are identical in Trichomonas vaginalis [6].
CAS REGISTRY NUMBER
COMMENTARY hide
9000-97-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-aspartate + 2-oxoglutarate
oxaloacetate + L-glutamate
show the reaction diagram
L-kynurenine + ?
kynurenic acid + ?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-aspartate + 2-oxoglutarate
oxaloacetate + L-glutamate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
a pyridoxal 5'-phosphate protein
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutaconate
-
injection into mice leads to inhibition of the kidney enzyme
aminooxyacetic acid hemihydrochloride
-
inhibitor of C-S lyase, reduces renal injuries due to cisplatin in rats. On day 5 following a bolus cisplatin injection, in vivo nephrotoxic potentials are in the decreasing order of species rats > mice, rabbits, based on body surface. The levels of renal Pt residue at the nephrotoxic dose are in order of rabbits > rats > mice. The activity of endogenous basal mitochondrial aspartate aminotransferase, one of the C-S lyases, in the renal cortex of naive animals is rats > mice, rabbits. Expression of mitochondrial C-S lyase in the kidney is observed at approximately 37 kDa in all five species used. In in vitro studies, the cytotoxicity of cisplatin is dependent on the expression level of C-S lyase mRNA in the respective renal cells
additional information
-
acarbose causes no changes in the specific and total activities of AST
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
889 units/g protein
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
decreased enzyme activity induces calpain activation by increasing the cytosolic Ca2+ concentrations of BV2 microglia, resulting in mitochondrial depolarization and cell death
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AATC_MOUSE
413
0
46248
Swiss-Prot
other Location (Reliability: 4)
AATM_MOUSE
430
0
47411
Swiss-Prot
Mitochondrion (Reliability: 2)
AATC2_MOUSE
404
0
45458
Swiss-Prot
other Location (Reliability: 3)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
circulating osteocalcin concentration is negatively associated with aspartate transaminase levels
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
the inhibitor of C-S lyase, aminooxyacetic acid hemihydrochloride, reduces renal injuries due to cisplatin in rats. On day 5 following a bolus cisplatin injection, in vivo nephrotoxic potentials are in the decreasing order of species rats > mice, rabbits, based on body surface. The levels of renal Pt residue at the nephrotoxic dose are in order of rabbits > rats > mice. The activity of endogenous basal mitochondrial aspartate aminotransferase, one of the C-S lyases, in the renal cortex of naive animals is rats > mice, rabbits. Expression of mitochondrial C-S lyase in the kidney is observed at approximately 37 kDa in all five species used. In in vitro studies, the cytotoxicity of cisplatin is dependent on the expression level of C-S lyase mRNA in the respective renal cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kato, Y.; Asano, Y.; Makar, T.K.; Cooper, A.J.
Irreversible inactivation of aspartate aminotransferase by 2-oxoglutaconic acid and its dimethyl ester
J. Biochem.
120
531-539
1996
Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Petlevski, R.; Hadzija, M.; Bajalo, J.L.; Juretic, D.
Effect of acarbose on alanine aminotransferase and aspartate aminotransferase activities in the liver of control and diabetic CBA mice
Acta Pharm.
56
87-93
2006
Mus musculus
Manually annotated by BRENDA team
Guidetti, P.; Amori, L.; Sapko, M.T.; Okuno, E.; Schwarcz, R.
Mitochondrial aspartate aminotransferase: a third kynurenate-producing enzyme in the mammalian brain
J. Neurochem.
102
103-111
2007
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Fernandez-Real, J.M.; Ortega, F.; Gomez-Ambrosi, J.; Salvador, J.; Fruehbeck, G.; Ricart, W.
Circulating osteocalcin concentrations are associated with parameters of liver fat infiltration and increase in parallel to decreased liver enzymes after weight loss
Osteoporos. Int.
21
2101-2107
2010
Mus musculus
Manually annotated by BRENDA team
Katayama, R.; Nagata, S.; Iida, H.; Yamagishi, N.; Yamashita, T.; Furuhama, K.
Possible role of cysteine-S-conjugate beta-lyase in species differences in cisplatin nephrotoxicity
Food Chem. Toxicol.
49
2053-2059
2011
Oryctolagus cuniculus, Mus musculus, Rattus norvegicus (P00507)
Manually annotated by BRENDA team
Wang, C.; Chen, H.; Ying, W.
Cytosolic aspartate aminotransferase mediates the mitochondrial membrane potential and cell survival by maintaining the calcium homeostasis of BV2 microglia
NeuroReport
29
99-105
2018
Mus musculus
Manually annotated by BRENDA team