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Information on EC 2.5.1.18 - glutathione transferase and Organism(s) Bos taurus

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IUBMB Comments
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
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Bos taurus
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The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
hide
RX
+
glutathione
=
HX
+
R-S-glutathione
+
=
+
Synonyms
gst, glutathione s-transferase, gstm1, gstp1, gstt1, glutathione-s-transferase, glutathione transferase, gsta1, gst pi, gstm3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha class glutathione transferase
-
glutathione S-alkyl transferase
-
-
-
-
glutathione S-aralkyltransferase
-
-
-
-
glutathione S-aryltransferase
-
-
-
-
glutathione S-transferase
glutathione S-transferase X
-
-
-
-
GSH S-transferase
-
-
-
-
GSHTase-P
-
-
-
-
GST
-
-
GST A1-1
-
isozyme
GSTA1-1
isozyme
S-(hydroxyalkyl)glutathione lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aryl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
RX:glutathione R-transferase
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-37-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + 1,2-diiodoethane
?
show the reaction diagram
best substrate
-
-
?
glutathione + 1,2-dinitrobenzene
?
show the reaction diagram
-
-
-
-
?
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
?
show the reaction diagram
-
-
-
-
?
glutathione + 1,4-diiodobutane
?
show the reaction diagram
-
-
-
?
glutathione + 1,6-diiodohexane
?
show the reaction diagram
-
-
-
?
glutathione + 1,8-diiodooctane
?
show the reaction diagram
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
glutathione + 1-chloro-2,4-dinitrobenzene
S-2,4-dinitrophenylglutathione + HCl
show the reaction diagram
-
-
-
-
?
glutathione + 1-iodohexane
?
show the reaction diagram
-
-
-
?
glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole
?
show the reaction diagram
-
-
-
-
?
glutathione + 3,4-dinitrobenzoic acid
?
show the reaction diagram
-
-
-
-
?
glutathione + 3-iodopropylbenzene
?
show the reaction diagram
-
-
-
?
glutathione + 4-nitrobenzyl chloride
?
show the reaction diagram
-
-
-
-
?
glutathione + 5-nitrofurfural
?
show the reaction diagram
-
-
-
-
?
glutathione + 5-nitrofurfural diacetal
?
show the reaction diagram
-
-
-
-
?
glutathione + azathioprine
6-mercaptopurine + ?
show the reaction diagram
-
-
-
-
?
RX + glutathione
HX + R-S-glutathione
show the reaction diagram
-
RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
butanol
-
50% inactivation at 5%
Cd2+
-
-
Co2+
-
weak
Cu2+
-
-
Hg2+
-
-
Ni2+
-
weak
Zn2+
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
1,2-dinitrobenzene
-
isozymes I and II
0.55 - 1.8
1-chloro-2,4-dinitrobenzene
1.3
3,4-dinitrobenzoic acid
-
isozymes I and II
0.1 - 0.3257
glutathione
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
190.6
1-chloro-2,4-dinitrobenzene
-
-
7.95
glutathione
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.005
-
isozyme GST A1-1, using azathioprine as a substrate
0.24
using iodohexane as substrate
0.27
using 3-iodopropylbenzene as substrate
0.38
using 1,6-diiodohexane as substrate
0.87
using 1,4-diiodobutane as substrate
12.72
-
more anionic isoenzyme
15
-
with 1-chloro-2,4-dinitrobenzene
15.47
-
less anionic isoenzyme
2.3
-
with 1,2-dinitrobenzene
26.6
using 1,2-diiodoethane as substrate
4.58
-
isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
more anionic transferase
6.5 - 7.5
-
less anionic transferase
7.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
pH 6: about 40% of activity maximum, pH 8: about 55% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GSTT1_BOVIN
240
0
27149
Swiss-Prot
other Location (Reliability: 2)
GSTM1_BOVIN
218
0
25635
Swiss-Prot
Secretory Pathway (Reliability: 5)
MGST1_BOVIN
155
3
17645
Swiss-Prot
other Location (Reliability: 2)
MGST2_BOVIN
146
2
16483
Swiss-Prot
Secretory Pathway (Reliability: 2)
GSTP1_BOVIN
210
0
23613
Swiss-Prot
other Location (Reliability: 3)
GSTA1_BOVIN
222
0
25452
Swiss-Prot
other Location (Reliability: 5)
GSTA2_BOVIN
223
0
25717
Swiss-Prot
other Location (Reliability: 5)
PTGES_BOVIN
153
2
17298
Swiss-Prot
Secretory Pathway (Reliability: 2)
GSTA4_BOVIN
222
0
25659
Swiss-Prot
other Location (Reliability: 4)
LANC1_BOVIN
399
0
45309
Swiss-Prot
other Location (Reliability: 2)
A0A3Q1MQV0_BOVIN
218
0
25842
TrEMBL
Secretory Pathway (Reliability: 5)
E1BH17_BOVIN
218
0
25688
TrEMBL
Secretory Pathway (Reliability: 5)
Q1JQ90_BOVIN
222
0
25650
TrEMBL
Secretory Pathway (Reliability: 5)
F1MKB7_BOVIN
241
0
27199
TrEMBL
Mitochondrion (Reliability: 5)
F1MX44_BOVIN
222
0
25369
TrEMBL
other Location (Reliability: 4)
A4IFG0_BOVIN
218
0
25780
TrEMBL
Secretory Pathway (Reliability: 5)
A1A4L7_BOVIN
218
0
25670
TrEMBL
other Location (Reliability: 5)
G3MZB0_BOVIN
132
0
14615
TrEMBL
Mitochondrion (Reliability: 5)
A0A3Q1MJD4_BOVIN
224
1
26200
TrEMBL
other Location (Reliability: 4)
Q9TRQ0_BOVIN
21
0
2493
TrEMBL
other Location (Reliability: 2)
E1BJ08_BOVIN
344
0
38033
TrEMBL
other Location (Reliability: 2)
A0A3S5ZPD5_BOVIN
281
0
31633
TrEMBL
other Location (Reliability: 3)
A5PKM0_BOVIN
229
0
26842
TrEMBL
Secretory Pathway (Reliability: 3)
E1BED9_BOVIN
244
0
28261
TrEMBL
other Location (Reliability: 1)
F1MRG0_BOVIN
226
0
25666
TrEMBL
Secretory Pathway (Reliability: 5)
F6Q751_BOVIN
218
0
25654
TrEMBL
Secretory Pathway (Reliability: 5)
A0A3Q1LMV5_BOVIN
240
0
26746
TrEMBL
other Location (Reliability: 3)
A0A140T854_BOVIN
222
0
25425
TrEMBL
other Location (Reliability: 5)
A0A3Q1LT12_BOVIN
303
0
35015
TrEMBL
other Location (Reliability: 5)
A0A3Q1M0K8_BOVIN
236
0
27349
TrEMBL
Mitochondrion (Reliability: 3)
A0A3Q1MHS2_BOVIN
251
1
28725
TrEMBL
Secretory Pathway (Reliability: 4)
Q32L24_BOVIN
222
0
25673
TrEMBL
other Location (Reliability: 4)
A5PKL9_BOVIN
155
3
17640
TrEMBL
other Location (Reliability: 3)
A0A3Q1LSN6_BOVIN
240
0
28447
TrEMBL
other Location (Reliability: 3)
F1MXY2_BOVIN
155
3
17591
TrEMBL
other Location (Reliability: 3)
F1MUX5_BOVIN
218
0
25596
TrEMBL
other Location (Reliability: 5)
A8E4P1_BOVIN
155
3
17622
TrEMBL
other Location (Reliability: 3)
Q2KIW8_BOVIN
226
0
25677
TrEMBL
Secretory Pathway (Reliability: 5)
E1BKU3_BOVIN
142
0
15917
TrEMBL
other Location (Reliability: 4)
A0A3Q1LLL0_BOVIN
239
0
27038
TrEMBL
Mitochondrion (Reliability: 3)
A0A452DHY4_BOVIN
191
0
21603
TrEMBL
other Location (Reliability: 1)
Q2KIV8_BOVIN
225
0
26850
TrEMBL
other Location (Reliability: 4)
Q0VCE1_BOVIN
143
0
15808
TrEMBL
Mitochondrion (Reliability: 5)
E1BJX2_BOVIN
241
0
28076
TrEMBL
other Location (Reliability: 4)
A0A3Q1MEC8_BOVIN
291
0
32384
TrEMBL
other Location (Reliability: 5)
E1BGC0_BOVIN
215
0
24470
TrEMBL
other Location (Reliability: 4)
Q0VCS8_BOVIN
239
0
27277
TrEMBL
other Location (Reliability: 3)
E1BKD8_BOVIN
224
0
25480
TrEMBL
other Location (Reliability: 4)
Q9TQX5_BOVIN
20
0
2292
TrEMBL
other Location (Reliability: 1)
A5PJE0_BOVIN
222
0
25394
TrEMBL
other Location (Reliability: 4)
A0A3Q1LFR2_BOVIN
218
0
25656
TrEMBL
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
2 * 27000, SDS-PAGE
49000
-
gel filtration
53000
-
native enzyme, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 27000, SDS-PAGE
homodimer
-
2 * 27000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
10 min, 37°C, 50% loss of activity, 18 h at 4°C, 50% loss of activity
637901
6 - 10
-
10 min at 37°C, stable, 18 h at 4°C, stable
637901
6.5
-
stable at pH 6.5 in potassium phosphate buffer
689896
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
3 h, stable
35
-
1 h, stable
37
-
fairly stable between pH 6 and 10
40
-
10 min, stable
55
-
above, 10 min, 50% inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 30%, protects against thermal inactivation
-
sucrose, 0.5 M, protects against thermal inactivation
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
Butanol
-
stable at 5% butanol
dimethyl sulfoxide
-
stable at 5% and 25% dimethyl sulfoxide
dimethylformamide
-
5% dimethylformamide: stable, 25% dimethylformamide: 70-80% inactivation
dioxane
Ethanol
Methanol
methyl cellosolve
-
stable at 5% and 25% methyl cellosolve for 2 h
propanol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 50% loss of activity after 18 h
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes
-
glutathione agarose affinity chromatography
-
Ni-IMAC affinity column chromatography
nickel-immobilized metal ion affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli XL-1 Blue cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Asaoka, K.
Affinity purification and characterization of glutathione S-transferases from bovine liver
J. Biochem.
95
685-696
1984
Bos taurus
Manually annotated by BRENDA team
Saneto, R.P.; Awasthi, Y.C.; Srivastava, S.K.
Glutathione S-transferases of the bovine retina. Evidence that glutathione peroxidase activity is the result of glutathione S-transferase
Biochem. J.
205
213-217
1982
Bos taurus
Manually annotated by BRENDA team
Kurtovic, S.; Grehn, L.; Karlsson, A.; Hellman, U.; Mannervik, B.
Glutathione transferase activity with a novel substrate mimics the activation of the prodrug azathioprine
Anal. Biochem.
375
339-344
2008
Bos taurus, Homo sapiens, Homo sapiens (P08263), Homo sapiens (Q16772), Rattus norvegicus (P04903), Rattus norvegicus (P04904)
Manually annotated by BRENDA team
Kurtovic, S.; Shokeer, A.; Mannervik, B.
Diverging catalytic capacities and selectivity profiles with haloalkane substrates of chimeric alpha class glutathione transferases
Protein Eng. Des. Sel.
21
329-341
2008
Rattus norvegicus (P04903), Rattus norvegicus (P04904), Homo sapiens (P08263), Homo sapiens (P09210), Homo sapiens (Q16772), Homo sapiens, Bos taurus (Q28035), Bos taurus
Manually annotated by BRENDA team
Guevercin, S.; Erat, M.; Sakiroqlu, H.
Determination of some kinetic and characteristic properties of glutathione S-transferase from bovine erythrocytes
Protein Pept. Lett.
15
6-12
2008
Bos taurus
Manually annotated by BRENDA team