Information on EC 2.4.99.8 - alpha-N-acetylneuraminate alpha-2,8-sialyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.99.8
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RECOMMENDED NAME
GeneOntology No.
alpha-N-acetylneuraminate alpha-2,8-sialyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - ganglio series
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Glycosphingolipid biosynthesis - globo series
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Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:alpha-N-acetylneuraminyl-(2->3)-beta-D-galactoside alpha-(2->8)-N-acetylneuraminyltransferase
Gangliosides act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
67339-00-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cichlid fish
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-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Xenopus (Silurana) tropicalis
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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polysialyltransferase ST8SiaIV knockout mice display a decreased motivation in social interaction. This deficit can be partly explained by olfactory deficits and was associated with a clear decrease in acid polysialic acid-neuronal cell adhesion molecule expression in brain. Sialyltransferase-X knockout mice display both a decreased social motivation and an increased aggressive behavior and show mild increase of polysialic acid-neuronal cell adhesion molecule expression in the lateral septum and the orbitofrontal cortex
metabolism
physiological function
sensory experience-dependent ST8SiaII gene expression regulates polysialic acid levels in postnatal visual cortex, thus acting as molecular link between visual activity and polysialic acid expression
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CMP-alpha-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
CMP-alpha-N-acetylneuraminate + NeuAc-alpha2,8-NeuAc-alpha2,3-Gal-beta1,4-Glc-beta-FCHASE
GMP + ?
show the reaction diagram
CMP-N-acetylneuraminate + (11-azidoundecyl 5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosid)onic acid
CMP + 11-azidoundecyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid + 11-azidoundecyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid + 11-azidoundecyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid + 11-azidoundecyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid + 11-azidoundecyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid
show the reaction diagram
CMP-N-acetylneuraminate + (methyl S-5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onic acid
CMP + methyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid + methyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid
show the reaction diagram
CMP-N-acetylneuraminate + 4-chlorophenyl 6-S-(5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->6)-1,6-dithio-beta-D-galactopyranoside
CMP + 4-chlorophenyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->6)-S-1,6-dithio-beta-D-galactopyranoside
show the reaction diagram
CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
show the reaction diagram
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i.e. sialosylneolactotetraosylceramide or ganglioside LM1
i.e. disialosylneolactotetraosylceramide or ganglioside LD1c
?
CMP-N-acetylneuraminate + beta-sialic acid
CMP + alpha-N-acetylneuraminyl-(2->8)-beta-N-acetylneuraminate
show the reaction diagram
CMP-N-acetylneuraminate + bovine submaxillary mucin
CMP + ?
show the reaction diagram
substrate for isoform ST8Sia-VI, 100% activity
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-
?
CMP-N-acetylneuraminate + disialoganglioside GD1a
CMP + trisialoganglioside GT1a
show the reaction diagram
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-
-
?
CMP-N-acetylneuraminate + ganglioside GM3
CMP + ?
show the reaction diagram
CMP-N-acetylneuraminate + methyl S-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->6)-(6-thio-beta-D-galactopyranosyl)-(1->4)-O-beta-D-glucopyranoside
CMP + methyl O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->8)-(5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-non-2-ulopyranosylonic acid)-(2->6)-(6-thio-beta-D-galactopyranosyl)-(1->4)-beta-D-glucopyranoside
show the reaction diagram
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19% yield
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?
CMP-N-acetylneuraminate + N-acyl-lyso-GM3
CMP + N-acyl-lyso-GD3
show the reaction diagram
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N-acetyl derivative is a better substrate than GM3, detergent-like effect
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?
CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
CMP-N-acetylneuraminate + N-butyrylneuraminyl-alpha-2,3-galactosyl-beta-1,4-glucosyl-beta-1,1-ceramide
CMP + ?
show the reaction diagram
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sialylated at about 60% the rate of GM3
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-
?
CMP-N-acetylneuraminate + N-glycolylneuraminyl-alpha-2,3-galactosyl-beta-1,4-glucosyl-beta-1,1-ceramide
CMP + ?
show the reaction diagram
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-
-
-
?
CMP-N-acetylneuraminate + neural cell adhesion molecule
CMP + ?
show the reaction diagram
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-
-
?
CMP-N-acetylneuraminate + neuropilin-2
CMP + ?
show the reaction diagram
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-
-
?
CMP-N-acetylneuraminate + trisialoganglioside GT1b
CMP + ganglioside GQ1b
show the reaction diagram
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-
-
?
CMP-Neu5Ac + GT3-FCHASE
CMP + long polySia chains + ?
show the reaction diagram
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the trisialylganglioside analogue GT3-FCHASE as artificial acceptor substrate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CMP-alpha-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
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branch-point enzyme in ganglioside biosynthetic sequence
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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required
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
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less effective than CMP or GMP
Cd2+
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strong
CDP
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only partially relieved by excess Mg2+
CMP
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strong
Ganglioside D1a
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Ganglioside LM1
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at higher concentrations, substrate inhibition
Ganglioside Q1b
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strong
Ganglioside T1b
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GMP
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as strong as CMP
Lysophospholipids
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-
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N-ethylmaleimide
-
-
TMP
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less effective than AMP
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Digitonin
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histone
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slight activation
Myrj 59
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activation, most potent activator, can be replaced by the following detergents, descending efficiency: sodium deoxycholate, Triton CF-54, Tween 20, Tween 80/Triton CF-54 ratio 1:*2, Triton X-100 or Tween 80
Nonidet P-40
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activation
Triton CF-54
Triton X-100
Zwittergent 3-10
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Zwittergent 3-14
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additional information
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the presence of detergents is essential for activity, no activation by phosphatidylglycerol
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4 - 3.2
CMP-alpha-N-acetylneuraminate
0.07 - 0.8
CMP-N-acetylneuraminate
0.42
CMP-Neu5Ac
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calculated and determined at constant donor concentration of 1 mM CMPNeu5Ac
0.078 - 0.979
ganglioside GM3
1
ganglioside GT1a
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-
-
0.063
Ganglioside LM1
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soluble enzyme preparation
0.12 - 0.145
NeuAc-alpha2,8-NeuAc-alpha2,3-Gal-beta1,4-Glc-beta-FCHASE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00028 - 0.00055
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-
additional information
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the specific activity of both fusion proteins is increased 2fold compared with enzymes with short tags
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.2
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trisialoganglioside formation
6.5
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GM3 or GT1b as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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in primary neurons and astrocytes lacking GD3 synthase, amyloid beta-induced cell death and amyloid beta aggregation are inhibited
Manually annotated by BRENDA team
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tissue samples of primary invasive breast cancer cases
Manually annotated by BRENDA team
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the GD3 synthase gene may be involved in early tooth development, particularly in the proliferation of dental epithelium
Manually annotated by BRENDA team
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human vascular endothelial cell line, overexpression of enzyme results in accelerated apoptosis accompanied by reduced phosphorylation of AKT and cyclic-AMP responsive element binding protein
Manually annotated by BRENDA team
expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR
Manually annotated by BRENDA team
normal quantity of ST8Sia I_short mRNA; traces of long form of ST8Sia mRNA together with a normal quantity of the short one; traces of of ST8Sia I_long mRNA
Manually annotated by BRENDA team
expression detected by RT-PCR; expression detected by RT-PCR
Manually annotated by BRENDA team
low expression of ST8Sia I_short mRNA; very low expression of short form of ST8Sia I mRNA
Manually annotated by BRENDA team
expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR; expression detected by RT-PCR
Manually annotated by BRENDA team
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nuclear factor NF-kappaB plays an essential role in the transcriptional activity of human GD3 synthase gene
Manually annotated by BRENDA team
marked presence of of ST8Sia I_short mRNA; marked presence of short form of ST8Sia I mRNA
Manually annotated by BRENDA team
enzyme is weakly expressed during nervous system development, and shows a highly dynamic expression pattern in somites and somite-derived structures
Manually annotated by BRENDA team
low expression of ST8Sia I_short mRNA; very low expression of short form of ST8Sia I mRNA
Manually annotated by BRENDA team
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the enzyme is highly expressed during the early stage of tooth germ development (embryonic day 14.5), especially in dental epithelia, not in dental mesenchymal tissue
Manually annotated by BRENDA team
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up-regulation of hST8Sia III via phosphoinositide 3 kinase/AKT pathway results in the neuronal differentiation of U-87 cells by inducing expression of beta-tubulin III
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
50% of the wild-type polyST is soluble and enzymatically active, whereby the detected activity is 3fold higher in the soluble than in the insoluble fraction
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
SDS-polyacrylamide-gel electrophoresis in the presence of beta-mercaptoethanol
55000
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SDS-gel electrophoresis
95000
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SDS-polyacrylamide-gel electrophoresis in the absence of beta-mercaptoethanol
100000
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recombinant MBP-NmB-polyST, affinity chromatography and gel filtration, SDS-PAGE, Western blot analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
subsequent structure-function analyses of NmB-polyST based on refined sequence alignments allowed the identification of two functional motifs in bacterial sialyltransferases. Both (D/E-D/E-G and HP motif) are highly conserved among different sialyltransferase families with otherwise little or no sequence identity. Removal of the C-terminal extension present in NmB but not in the homologous Escherichia coli enzymes, completely abolished enzymatic activity, proving it as an essential functional domain. Using site-directed mutagenesis and refined protein alignment strategies, identify two functionally important motifs, which are highly conserved in a number of bacterial (poly)sialyltransferases of otherwise unrelated sequences. T7-polyST, NusA-polyST, MBP-polyST, and Strep II-polyST: NmB-polyST fusion proteins with large fusion partners (MBP, NusA) are used
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
secondary structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase. In the human enzyme, the side chain on residue N188 has a strong hydrogen bond with the carboxyl group on the sialic acid group of the donor substrate. Residue P189 has no interaction with the donor. The distance between S190 and the donor substrate is 4.4 A, this residue might weakly interact with the donor. R272 is 8.8 A away from the donor, suggesting that this residue has no function on donor substrate binding
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
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20% loss of activity after 120 s
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity and size exclusion chromatography
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homogenized, centrifuged, resuspended in 25mM-cacodylate buffer pH 6.5 containing 0.15% Triton X-100, 75mM NaCl and 10 mM MnCl2
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solubilized with Triton X-100, CDP-Sepharose affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Xenopus (Silurana) tropicalis
cloning and nucleotide sequences of ST8Sia I_long cDNA; cloning and nucleotide sequences of ST8Sia I_short; expression in COS-7 cell
cloning and sequence analysis of the 5'-flanking region of human GD3 synthase gene
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expressed in Escherichia coli BL21(DE3)
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expression in CHO-K1 cells
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expression in CHO-K1 cells. Secretion of mouse interleukin-2 signal sequence and transmembrane domain truncated human GD3-synthase cDNA amino-acid residues 49-356
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expression in CHOP cells
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expression in Escherichia coli
expression in K-562 cell
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expression in neuroblastoma cell line F-11
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expression in neuroblastoma cell line NG108-15
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expression in newborn brain in mouse
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expression of cDNA from melanoma cell line WM266-4 in Namalwa KJM-1 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
both isoforms ST8SiaII mRNA level decreases around the time of eye opening in mouse visual cortex; isoform ST8SiaIV mRNA level decreases around the time of eye opening in mouse visual cortex
higher expression in estrogen receptor negative breast tumors, gene expression of ganglioside GD3 synthase is associated with prognosis in breast cancer
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isoforms ST8SiaIV mRNA level is positively regulated by PKC-mediated signaling; isoform ST8SiaII mRNA level is positively regulated by protein kinase C-mediated signaling
no significant differences between the groups with high and low ST8SIA expression for age, tumor size, lymph node status, and Her 2 neu overexpression of patients
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA32I53S
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truncated mutant. In addition to the alpha2,3- and alpha2,8-sialyltransferase activities of wild-type for the synthesis of GM3- and GD3-type oligosaccharides, respectively. The CstII DELTA32I53S mutant has alpha2,8-sialyltransferase, i. e. GT3 oligosaccharide synthase activity for the synthesis of GT3 oligosaccharide. It also has alpha2,8-sialidase i.e. GD3 oligosaccharide sialidase activity that catalyzes the specific cleavage of the alpha2,8-sialyl linkage of GD3-type oligosaccharides and alpha2,8-trans-sialidase, i. e. GD3 oligosaccharide trans-sialidase activity that catalyzes the transfer of a sialic acid from a GD3 oligosaccharide to a different GM3 oligosaccharide
H331K
catalytically inactive
N188D
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mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 6fold decrease in ratio Km to Vmax
R272A
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mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 4fold decrease in ratio Km to Vmax
S190A
-
mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 4fold decrease in ratio Km to Vmax
A281V
the mutant of isoform ST8Sia-I shows 103.7% activity with ganglioside GM3
A328V
the mutant of isoform ST8Sia-VI shows 77.2% activity with bovine submaxillary mucin
C286A
the mutant of isoform ST8Sia-I shows no activity with ganglioside GM3
C335A
the mutant of isoform ST8Sia-VI shows no activity with bovine submaxillary mucin
E288N
the mutant of isoform ST8Sia-I shows 66.5% activity with ganglioside GM3
E333G
the mutant of isoform ST8Sia-VI shows no activity with bovine submaxillary mucin
F277M
the mutant of isoform ST8Sia-I shows 81.1% activity with ganglioside GM3
G284E
the mutant of isoform ST8Sia-I shows no activity with ganglioside GM3
I291L
the mutant of isoform ST8Sia-I shows 95.3% activity with ganglioside GM3
I327L
the mutant of isoform ST8Sia-VI shows 90% activity with bovine submaxillary mucin
K339S
the mutant of isoform ST8Sia-VI shows 34.6% activity with bovine submaxillary mucin
L278I
the mutant of isoform ST8Sia-I shows 11.9% activity with ganglioside GM3
L283V
the mutant of isoform ST8Sia-I shows 45.4% activity with ganglioside GM3
L340I
the mutant of isoform ST8Sia-VI shows 18.6% activity with bovine submaxillary mucin
M326F
the mutant of isoform ST8Sia-VI shows no activity with bovine submaxillary mucin
N337E
the mutant of isoform ST8Sia-VI shows no activity with bovine submaxillary mucin
S273A
the mutant of isoform ST8Sia-I shows no activity with ganglioside GM3
S290K
the mutant of isoform ST8Sia-I shows 72.4% activity with ganglioside GM3
S322A
the mutant of isoform ST8Sia-VI shows no activity with bovine submaxillary mucin
V279A
the mutant of isoform ST8Sia-I shows 85.6% activity with ganglioside GM3
V330A
the mutant of isoform ST8Sia-VI shows 92% activity with bovine submaxillary mucin
V332L
the mutant of isoform ST8Sia-VI shows 69.8% activity with bovine submaxillary mucin
W295A
the mutant of isoform ST8Sia-I shows 3.3% activity with ganglioside GM3; the mutant of isoform ST8Sia-I shows 3.3% activity with ganglioside GM3
W344A
the mutant of isoform ST8Sia-VI shows no activity with bovine submaxillary mucin
E153A
-
inactive, single-point mutation of NmB-polyST by QuickChange site-directed mutagenesis
G154A
-
inactive, single-point mutation of NmB-polyST by QuickChange site-directed mutagenesis
H278A
-
nearly inactive, single-point mutation of NmB-polyST by QuickChange site-directed mutagenesis. Vmax value for CMP-Neu5Ac is decreased by a factor 6 with respect to the wild-type enzyme and the Km value for CMP-Neu5Ac is 5fold
H278A/P279A
-
the H278A and P279A mutants maintain residual activity (below 10% of wild type), when both residues are changed to alanine simultaneously (H278A/P279A) enzyme activity is abolished
P279A
-
nearly inactive, single-point mutation of NmB-polyST by QuickChange site-directed mutagenesis. Vmax value for CMP-Neu5Ac is decreased by a factor of 4 with respect to the wild-type enzyme and the Km values for CMP-Neu5Ac is increased 3fold
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
putative prognostic marker in breast cancer, estrogen receptor negative patients with high ceramide kinase expression had a worse prognosis then those with low expression
drug development
-
basis for design of sialyltransferase-specific drugs
medicine