Information on EC 2.4.99.1 - beta-galactoside alpha-2,6-sialyltransferase

New: Word Map on EC 2.4.99.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.99.1
-
RECOMMENDED NAME
GeneOntology No.
beta-galactoside alpha-2,6-sialyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
show the reaction diagram
mechanism
-
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
show the reaction diagram
The terminal beta-D-galactosyl residue of the oligosaccharide of glycoproteins, as well as lactose, can act as acceptor
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
show the reaction diagram
Val220 participates in binding the common cytidine moiety in substrate CMP-NeuAc and inhibitor CDP
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
N-Glycan biosynthesis
-
-
Other types of O-glycan biosynthesis
-
-
terminal O-glycans residues modification
-
-
SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine alpha-(2->6)-N-acetylneuraminyltransferase
The terminal beta-D-galactosyl residue of the oligosaccharide of glycoproteins, as well as lactose, can act as acceptor.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
alpha 2,6-ST
-
-
-
-
alpha2-6 sialyltransferase
-
-
-
-
antigens, CD75
-
-
-
-
B-cell antigen CD75
-
-
-
-
CMP-acetylneuraminate-galactosylglycoprotein sialyltransferase
-
-
-
-
CMP-acetylneuraminate-glycoprotein sialyltransferase
-
-
-
-
CMP-N-acetylneuraminic acid-glycoprotein sialyltransferase
-
-
-
-
cytidine monophosphoacetylneuraminate-galactosylglycoprotein sialyltransferase
-
-
-
-
sialotransferase
-
-
-
-
sialyltransferase
-
-
-
-
sialyltransferase, cytidine monophosphoacetylneuraminate-galactosylglycoprotein
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9075-81-4
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
fragment
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
ST6GAL1
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment
SwissProt
Manually annotated by BRENDA team
ST6GAL1
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment
SwissProt
Manually annotated by BRENDA team
ST6GAL1
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment
SwissProt
Manually annotated by BRENDA team
Helicobacter acinonychis ATCC 51104
-
SwissProt
Manually annotated by BRENDA team
recombinant
-
-
Manually annotated by BRENDA team
ST6GAL1
SwissProt
Manually annotated by BRENDA team
ST6GAL2
SwissProt
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
ST6Gal I; ST6Gal II
-
-
Manually annotated by BRENDA team
ST6GAL1
SwissProt
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
-
-
Manually annotated by BRENDA team
ST6GAL1; medaka fish, Japanese ricefish
SwissProt
Manually annotated by BRENDA team
ST6GAL2; medaka fish, Japanese ricefish
SwissProt
Manually annotated by BRENDA team
ST6GAL1, fragment; chimpanzee
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment; chimpanzee
SwissProt
Manually annotated by BRENDA team
Photobacterium damselae JT0160
JT0160
-
-
Manually annotated by BRENDA team
strain JT-SHIZ-119
-
-
Manually annotated by BRENDA team
Photobacterium leiognathi JT-SHIZ-119
strain JT-SHIZ-119
-
-
Manually annotated by BRENDA team
Photobacterium leiognathi JT-SHIZ-145
JT-SHIZ-145
-
-
Manually annotated by BRENDA team
JT-ISH-224
-
-
Manually annotated by BRENDA team
commercial product
-
-
Manually annotated by BRENDA team
isoform STcys; isoform STtyr
-
-
Manually annotated by BRENDA team
male Sprague-Dawley and Wistar rats
SwissProt
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
purified enzyme
-
-
Manually annotated by BRENDA team
purified enzyme; Sprague-Dawley
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
ST6GAL1
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment
SwissProt
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
ST6GAL1; i.e. Takifugu rubripes, Japanese pufferfish
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment; i.e. Takifugu rubripes, Japanese pufferfish
SwissProt
Manually annotated by BRENDA team
ST6GAL2, fragment; i.e. Silurana tropicalis, western clawed frog
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
enzyme down-regulation limits monocyte/macrophage lifespan by sensitizing cells to apoptosis via tumor necrosis factor receptor I hyposialylation
physiological function
A5D7T4
alpha2,6-sialylation mediated by ST6Gal II can contribute to organism's response to infections
physiological function
-
DELTA15Pd2,6ST-catalyzed trans-sialidase reaction follows a ping-pong bi-bi reaction mechanism. DELTA15Pd2,6ST also has alpha2,6-sialidase activity
physiological function
-
recombinant ST6Gal1 can be used as a key enzyme for the synthesis of an artificial sialoglycopolymer for potentially blocking influenza virus infection
physiological function
-
reduced sialylation of serum IgA1 may result from decreased expression of ST6GALNAC2
physiological function
-
shows both alpha2,6-sialyltransferase and alpha2,6-linkage-specific neuraminidase activity. Sialyltransferase activity of pLST-DELTAN is much higher than that of pLST-FL, and reaches 1477 U/l culture broth
physiological function
-
ST6Gal I knockout mice
physiological function
-
circulatory enzyme, mostly of hepatic origin, limits myelopoiesis by a mechanism independent of hepatic sialylation of serum glycoproteins
physiological function
-
enzyme overexpression in an U-373MG stable transfectant leads to elevated expression of GM2alpha and GM3 gangliosides and and decreased expression of GM1b, Gb3, and Gb4, marked inhibition of in vitro invasivity, modified cellular adhesion to fibronectin and laminin, increased adhesion-mediated protein tyrosine-phosphorylationof HSPA8, and inhibition of tumor growth in vivo
physiological function
-
the enzyme is responsible for alpha2,6-sialylated blood group type 2H antigen biosynthesis in human colon cancer cells
physiological function
-
the enzyme regulates macrophage tumor necrosis factor alpha-induced apoptosis via alpha2-6 sialylation of the tumor necrosis factor receptor I death receptor. The enzyme inhibits phorbol-12-myristate-13-acetate-induced suppression of cell growth
physiological function
Photobacterium leiognathi JT-SHIZ-119
-
shows both alpha2,6-sialyltransferase and alpha2,6-linkage-specific neuraminidase activity. Sialyltransferase activity of pLST-DELTAN is much higher than that of pLST-FL, and reaches 1477 U/l culture broth
-
physiological function
Mus musculus C57BL/6
-
ST6Gal I knockout mice
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
asialo-alpha1-acidglycoprotein + CMP-Neu5Ac
?
show the reaction diagram
A5D7T4
membrane-bound and soluble protein, 34% and 25% relative activity, respectively
-
-
?
CMP-9''-benzamido-Neu5Ac + lactose
?
show the reaction diagram
-
-
-
-
?
CMP-9''-benzamido-Neu5Ac + N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
CMP-9''-phenylacetamido-Neu5Ac + lactose
?
show the reaction diagram
-
-
-
-
?
CMP-9''-phenylacetamido-Neu5Ac + N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
CMP-9''-phenylhexanamido-Neu5Ac + lactose
?
show the reaction diagram
-
-
-
-
?
CMP-9''-phenylhexanamido-Neu5Ac + N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
CMP-9''-phenylpropionamido-Neu5Ac + lactose
?
show the reaction diagram
-
-
-
-
?
CMP-9''-phenylpropionamido-Neu5Ac + N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
CMP-9-acetamido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-9-amino-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-9-azido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-9-benzamido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-9-hexanoylamido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-9-O-acetyl-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
comparable or even higher transfer rates than with CMP-N-acetylneuraminate
-
-
?
CMP-9-O-acetyl-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
CMP + ?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + (2S,3S,4R,5R,6R)-5-acetylamino-4-hydroxy-6-methoxy-3-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-2-carboxylic acid methyl ester
CMP + ?
show the reaction diagram
-
160% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + (2S,3S,4R,5R,6R)-5-acetylamino-4-hydroxy-6-methoxy-3-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-2-carboxylic acid methylamide
CMP + ?
show the reaction diagram
-
0.2% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + (Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
CMP + ?
show the reaction diagram
-
50% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + (Gal-3-O-Me)beta(1-3)GlcNAcbeta-O-Me
CMP + ?
show the reaction diagram
-
80% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + 4-methylumbelliferyl beta-D-lactoside
?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + 4-nitrophenyl-D-galactoside
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-(4-nitro)phenol
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
CMP + ?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
CMP + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy)) ethyl beta-N-acetylneuraminic acid alpha-2,6-N-acetyllactosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + alpha1-acid glycoprotein
CMP + ?
show the reaction diagram
-
20.6% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + asialo-agalacto-prothrombin
CMP + agalacto-prothrombin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
CMP + ?
show the reaction diagram
-
12.3% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
CMP + alpha1-acid glycoprotein
show the reaction diagram
-
176% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + asialo-apoceruloplasmin
CMP + apoceruloplasmin
show the reaction diagram
-
best substrate
-
-
?
CMP-N-acetylneuraminate + asialo-ceruloplasmin
CMP + ceruloplasmin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-ceruloplasmin
CMP + ceruloplasmin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-chorionic gonadotropin
CMP + chorionic gonadotropin
show the reaction diagram
-
acceptor protein from human source
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
best substrate
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
106% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine, 21% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
-
recombinant isoform STcys is somewhat more active then the recombinant isoform STtyr
-
-
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
CMP-N-acetylneuraminate + asialo-fibrinogen
CMP + fibrinogen
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-immunoglobulin G
CMP + immunoglobulin G
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-immunoglobulin M
CMP + immunoglobulin M
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
best substrate
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
best substrate
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
CMP-N-acetylneuraminate can be replaced with comparable or even higher transfer rates by CMP-N-glycolylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
P13721
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
-
about half as effective as asialofetuin
-
-
?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
i.e. asialo-alpha1 acid glycoprotein
-
-
?
CMP-N-acetylneuraminate + asialo-proteose peptone
CMP + proteose peptone
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-prothrombin
CMP + prothrombin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-prothrombin
CMP + prothrombin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-prothrombin
CMP + prothrombin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-Tamm-Horsfall glycoprotein
CMP + Tamm-Horsfall glycoprotein
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-thyroglobulin
CMP + thyroglobulin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-thyroglobulin
CMP + thyroglobulin
show the reaction diagram
-
low activity
-
-
?
CMP-N-acetylneuraminate + asialo-thyroglobulin glycopeptide
CMP + thyroglobulin glycopeptide
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-transferrin
CMP + transferrin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-transferrin
CMP + transferrin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-transferrin
CMP + transferrin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-transferrin
CMP + transferrin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + asialo-transferrin
CMP + transferrin
show the reaction diagram
-
poor substrate
-
-
?
CMP-N-acetylneuraminate + asialoovomucoid
CMP + ?
show the reaction diagram
-
9.1% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
36% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosaminyl-1,3-galactosyl-beta-1,4-D-glucose
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosaminyl-1,3-galactosyl-beta-1,4-D-glucose
show the reaction diagram
-
106% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine, 129% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
F1SWB0
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
Q17WF8
highest activity
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
poor substrate
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
best substrate
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
Q64685
100% activity
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
most active disaccharide substrate
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
Photobacterium damselae JT0160
-
i.e. N-acetyllactosamine
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
Helicobacter acinonychis ATCC 51104
Q17WF8
highest activity
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine-R
show the reaction diagram
-
exhibits highest activity toward GalNAcbeta1,4GlcNAc carbohydrate structures at the non-reducing termini of oligosaccharides and glycoprotein glycans. Oligosaccharides are preferred over glycoproteins as acceptors, and no activity toward glycolipid acceptors is detected. Recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward beta-linked GalNAc termini of its own N-linked glycans
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-1,4-N-acetylglucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-1,4-N-acetylglucosamine
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,4-beta-D-galactosyl-1,4-beta-D-glucosyl-beta1-ceramide
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,4-beta-D-galactosyl-1,4-beta-D-glucosyl-beta1-ceramide
show the reaction diagram
-
i.e. paragloboside
i.e. alpha-2,6-sialylparagloboside
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
acceptor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to terminal positions on N-linked glycans
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to terminal positions on N-linked glycans
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to terminal positions on N-linked glycans
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to terminal positions on N-linked glycans
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to terminal positions on N-linked glycans
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to terminal positions on N-linked glycans
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
P13721
sialic acid is linked to C-6 of the galactose residue
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
transfers sialic acid to the terminal galactose residue of asialo-gyloproteins and peptides
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
CMP-N-acetylneuraminate analogues can serve as donor substrates, i.e. the 5''-amino-, the 5''-N-pentanoylamino-, the 5''-glycylamino-, and the 5''-ethoxycarbonylamino derivative and diammonium (cytidin-5'-yl) [(3-deoxy-D-glycero-beta-D-galacto-2-nonulpyranosyl)onate] phosphate
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
the non-reducing terminal galactosyl residue is essential for activity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
substrate specificity for several synthetic trisaccharides
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
high molecular weight substrates are more efficient acceptors than low molecular weight substrates
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
high molecular weight substrates are more efficient acceptors than low molecular weight substrates
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
high molecular weight substrates are more efficient acceptors than low molecular weight substrates
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
high molecular weight substrates are more efficient acceptors than low molecular weight substrates
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
synthetic disaccharides
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
CMP-N-acetylneuraminate analogues can serve as donor substrates, i.e. formyl-, trifluoroacetyl-, benzyloxycarbonyl-, and amino acetyl-N-neuraminates
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
donor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
donor substrate specificity
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
one of a group of glycosyltransferases which act to assemble the carbohydrate units of thyroglobulin
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
regulation of enzyme expression
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
final step in synthesis of serum-type glycoproteins
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
R: glycoprotein or glycopeptide
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
acceptor substrate specificity, sialic acid is linked to C-6 of the galactose residue, synthetic disaccharides
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
Photobacterium damselae JT0160
-
sialic acid is linked to C-6 of the galactose residue, strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
Photobacterium damselae JT0160
-
acceptor substrate specificity, sialic acid is linked to C-6 of the galactose residue, strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages
-
?
CMP-N-acetylneuraminate + fetuin
?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + fetuin
?
show the reaction diagram
-
no activity
-
-
-
CMP-N-acetylneuraminate + fetuin
?
show the reaction diagram
-
glycopeptides
-
-
?
CMP-N-acetylneuraminate + fetuin
?
show the reaction diagram
-
about 30% as effective as asialofetuin
-
-
?
CMP-N-acetylneuraminate + formic acid (2R,3R,4S,5R,6R)-2-((2R,3R,4R,5R,6R)-3-acetylamino-5-hydroxy-2-methoxy-6-methylaminomethyl-tetrahydro-pyran-4-yloxy)-4,5-dihydroxy-6-hydroxymethyl-tetrahydro-pyran-3-yl ester
CMP + ?
show the reaction diagram
-
as active as Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + fucosyllactose
CMP + alpha-N-acetylneuraminyl-2,6-[alpha-L-fucopyranosyl-1,2]-beta-D-galactopyranosyl-1,4-D-glucopyranose
show the reaction diagram
-
also pyridylaminated
-
?
CMP-N-acetylneuraminate + Gal-beta-1,3-GlcNAc-beta-OMe
?
show the reaction diagram
-
77% of the activity with lactose
-
-
?
CMP-N-acetylneuraminate + Galbeta(1-3)(GlcNAc-6-O-Me)beta-O-Me
CMP + ?
show the reaction diagram
-
80% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + Galbeta(1-3)-GlcNAcbeta(1-3)Galbeta(1-4)Glc
CMP + NeuNAcalpha(2-6)-Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
30% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + Galbeta(1-3)GlcNAcbeta-O-Me
CMP + NeuAcalpha(2-6)Galbeta(1-3)GlcNAcbeta-O-Me
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
CMP + alpha-N-acetylneuraminyl-(2-6)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
79% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + Galbeta(1->3)GalNAc
CMP + NeuAcalpha(2->6)Galbeta(1->3)GalNAc
show the reaction diagram
Q64685
10% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + Galbeta(1->3)GlcNAc
CMP + NeuAcalpha(2->6)Galbeta(1->3)GlcNAc
show the reaction diagram
Q64685
2% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + Galbeta(1->4)GlcNAcbeta(1->2)Manalpha(1->6)[Galbeta(1->4)GlcNAcbeta(1>2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc-pyridylamine
CMP + NeuAcalpha(2->6)Galbeta(1->4)GlcNAcbeta(1->2)Manalpha(1->6)[Galbeta(1->4)GlcNAcbeta(1>2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc-pyridylamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + GalNAcbeta1,4-GlcNAc-R
CMP + alpha-N-acetylneuraminyl-2,6-GalNAcbeta1,4-GlcNAc-R
show the reaction diagram
-
-
190% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
?
CMP-N-acetylneuraminate + lacto-N-neotetraose
?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + lacto-N-neotetraose
?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + lacto-N-neotetraose
CMP + ?
show the reaction diagram
Q64685
108% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + lacto-N-tetraose
CMP + ?
show the reaction diagram
-
36.8% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + lacto-N-tetraose
CMP + ?
show the reaction diagram
Q64685
35% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
no activity
-
-
-
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
poor substrate
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
poor substrate
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
A8QYL1
Asp232 might act as a catalytic base for deprotonation of the acceptor substrate, and His405 might act as a catalytic acid for protonation of the donor substrate
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
Q64685
14% activity compared to beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
Photobacterium damselae JT0160
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
Photobacterium damselae JT0160
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
Photobacterium leiognathi JT-SHIZ-145
-
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
A8QYL1
Asp232 might act as a catalytic base for deprotonation of the acceptor substrate, and His405 might act as a catalytic acid for protonation of the donor substrate
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + lactose
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-beta-D-glucose
show the reaction diagram
-
15.5% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + methanesulfonic acid (2R,3S,4R,5R,6R)-5-acetylamino-4-hydroxy-6-methoxy-3-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-2-ylmethyl ester
CMP + ?
show the reaction diagram
-
90% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + methyl-alpha-D-galactopyranoside
?
show the reaction diagram
-
2% of the activity with lactose
-
-
?
CMP-N-acetylneuraminate + methyl-beta-D-galactopyranoside
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-methylgalactopyranoside
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + methyl-beta-D-galactopyranoside
?
show the reaction diagram
-
59% of the activity with lactose
-
-
?
CMP-N-acetylneuraminate + N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.03,8]heptadec-16-yl)-acetamide
CMP + ?
show the reaction diagram
-
as active as Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18R)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-11-oxo-2,4,9,15-tetraoxa-12-aza-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
CMP + ?
show the reaction diagram
-
110% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18S)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-2,4,9,12,15-pentaoxa-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
CMP + ?
show the reaction diagram
-
130% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + N-acetyl-D-galactosamine
?
show the reaction diagram
-
12% of the activity with lactose
-
-
?
CMP-N-acetylneuraminate + N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + N-acetyllactosamine
?
show the reaction diagram
-
99% of the activity with lactose
-
-
?
CMP-N-acetylneuraminate + N-[(2R,3R,4R,5S,6R)-4-hydroxy-6-(methanesulfonylamino-methyl)-2-methoxy-5-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-3-yl]-acetamide
CMP + ?
show the reaction diagram
-
70% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + N-[(2R,3R,4R,5S,6R)-5-((2S,3R,4S,5R,6R)-4,5-dihydroxy-6-hydroxymethyl-3-methoxy-tetrahydro-pyran-2-yloxy)-6-formylaminomethyl-4-hydroxy-2-methoxy-tetrahydro-pyran-3-yl]-acetamide
CMP + ?
show the reaction diagram
-
as active as Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + N-[(2R,3R,4R,5S,6R)-6-(acetylamino-methyl)-4-hydroxy-2-methoxy-5-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-3-yl]-acetamide
CMP + ?
show the reaction diagram
-
60% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + N-[(2R,3R,4R,5S,6R)-6-aminomethyl-4-hydroxy-2-methoxy-5-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-3-yl]-acetamide
CMP + ?
show the reaction diagram
-
50% of the activity with Galbeta(1-3)GlcNAcbeta-O-Me
-
-
?
CMP-N-acetylneuraminate + Neu5Acalpha-2,3-Galbeta-1,4-Glc
CMP + alpha-N-acetylneuraminyl-2,6-(Neu5Acalpha-2,3-)Galbeta-1,4-Glc
show the reaction diagram
-
also pyridylaminated
-
-
?
CMP-N-acetylneuraminate + ovomucoid
CMP + ?
show the reaction diagram
-
8.4% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
?
CMP-N-acetylneuraminate + pyridylamine-type 2H
CMP + alpha2,6-sialylated blood group type 2H antigen
show the reaction diagram
-
2% activity compared to pyridylaminyl-lacto-N-neotetraose
-
-
?
CMP-N-acetylneuraminate + pyridylamino-lactose
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-beta-D-glucosyl-pyridylaminoside
show the reaction diagram
-
recombinant enzyme
-
-
?
CMP-N-acetylneuraminate + pyridylaminyl-lacto-N-neotetraose
CMP + ?
show the reaction diagram
-
100% activity
-
-
?
CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
show the reaction diagram
-
poor substrates, no activity with ovine acceptor
-
-
?
CMP-N-acetylneuraminate + tumor necrosis factor receptor 1
CMP + alpha2,6-sialyated tumor necrosis factor receptor 1
show the reaction diagram
-
-
-
-
?
CMP-N-alpha-neuraminate + ganglioside nLc4Cer
?
show the reaction diagram
-
-
-
-
?
CMP-N-alpha-neuraminate + ganglioside nLc6Cer
?
show the reaction diagram
-
-
-
-
?
CMP-N-alpha-neuraminate + pyridylaminated ganglioside nLc4Cer
?
show the reaction diagram
-
-
-
-
?
CMP-N-aminoacetylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-N-formylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
-
-
-
?
CMP-N-glycolylneuraminate + asialo-alpha1-acid glycoprotein
?
show the reaction diagram
-
comparable or even higher transfer rates than with CMP-N-acetylneuraminate
-
-
?
CMP-Neu5Ac + lactose
CMP + Neu5Acalpha(2-6)Lac
show the reaction diagram
Photobacterium leiognathi, Photobacterium leiognathi JT-SHIZ-119
-
alpha2,6-sialyltransferase activity
-
-
?
CMP-Neu5Ac + methyl-beta-D-galactopyranoside
CMP + Neu5Acalpha(2-6)Gal-alpha-methyl
show the reaction diagram
Photobacterium leiognathi, Photobacterium leiognathi JT-SHIZ-119
-
alpha2,6-sialyltransferase activity
-
-
?
CMP-Neu5Ac + N-acetyllactosamine
CMP + Neu5Acalpha(2-6)LacNAc
show the reaction diagram
Photobacterium leiognathi, Photobacterium leiognathi JT-SHIZ-119
-
alpha2,6-sialyltransferase activity
-
-
?
cytidine 5'-monophosphate 3-(axial)-fluoro-N-acetylneuraminic acid + alpha-lactose
?
show the reaction diagram
-
[15N]Phe-labeled ST6Gal-I with a 15fold excess of CMP-3FNeuAc
-
-
?
cytidine 5'-monophosphate 4-O-(4-carboxy-2,2,6,6-tetramethylpiperidine-1-oxyl) + alpha-N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
cytidine-5'-monophospho-sialic acid + lactose
?
show the reaction diagram
-
-
-
-
?
cytidine-5'-monophospho-sialic acid + N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
Galbeta(1,4)GlcNAc-octyl + CMP-Neu5Ac
?
show the reaction diagram
A5D7T4
membrane-bound and soluble protein, 100% relative activity
-
-
?
N-acetyllactosamine 4-O-(4-carboxy-2,2,6,6-tetramethylpiperidine-1-oxyl)
?
show the reaction diagram
-
[15N]Phe-labeled ST6Gal-I with a 20fold excess of N-acetyllactosamine 4-O-(4-carboxy-2,2,6,6-tetramethylpiperidine-1-oxyl)
-
-
?
Neu5Acalpha-4-nitrophenyl + 4-methylumbelliferyl-beta-D-lactoside
?
show the reaction diagram
-
alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST
-
-
?
GalNAcbeta(1,4)GlcNAc-octyl + CMP-Neu5Ac
?
show the reaction diagram
A5D7T4
membrane-bound and soluble protein, 312% and 346% relative activity, respectively
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
thyroglobin is no substrate
-
-
-
additional information
?
-
-
the catalytic domain can proceed with sialic acid transfer with increased efficiency until 80 amino acid are deleted
-
-
-
additional information
?
-
-
no activity with galactosylhydroxylysine and its derivatives, or galactose-containing oligosaccharides linked to Ser/Thr-glycopeptide of earthworm cuticle collagen, no transfer to terminal N-acetyl-D-galactosamine
-
-
-
additional information
?
-
-
no activity with beta-methyl-L-arabinopyranoside
-
-
-
additional information
?
-
-
no activity with submaxillary asialo-mucin
-
-
-
additional information
?
-
-
no activity with submaxillary asialo-mucin
-
-
-
additional information
?
-
-
no activity with submaxillary asialo-mucin, galactose-free glycopeptides are poor substrates
-
-
-
additional information
?
-
-
low activity with several methylated substrates, no activity with UDP-Gal, UDP-GalNAc and UDP-Glc as donor substrates
-
-
-
additional information
?
-
-
enzyme does not recognize type I oligosaccharides
-
-
-
additional information
?
-
-
very low activity with prothrombin and native alpha1-acid glycoprotein, no activity with transferrin, asialo-agalacto-alpha1-acid glycoprotein
-
-
-
additional information
?
-
-
no activity with N-acetyl-D-galactosamine or N-acetyl-D-glucosamine, and asialo-agalacto-fetuin, and galactose
-
-
-
additional information
?
-
-
no activity with antifreeze glycoprotein
-
-
-
additional information
?
-
-
no activity with antifreeze glycoprotein, poor substrates are the beta-1,3- or beta-1,6-derivatives of beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
-
additional information
?
-
-
no activity with galactose, lactose and N-acetyllactose
-
-
-
additional information
?
-
-
no activity with Lc4Cer and Lc3Cer
-
-
-
additional information
?
-
-
no activity with the CMP-NeuAc analogues disodium[(5-acetamido-3,5-dideoxy-D-glycero-beta-D-galacto-2-nonulopyranosyl)onate] (methyl-beta-D-ribofuranosid-5-yl) phosphate and bis(triethylammonium) [(5-acetamido-3,5-dideoxy-D-glycero-beta-D-galacto-2-nonulopyranosyl)onate] [(resorcin-4-yl) (1-deoxy-beta-D-ribofuranosid-5-yl)] phosphate
-
-
-
additional information
?
-
-
no activity with ovine submaxillary asialo-mucin
-
-
-
additional information
?
-
-
addition of alpha2,6-linked sialic acid to the Gal1,4GlcNAc sequence, the preferred ligand for galectin-1, inhibits recognition of this saccharide ligand by galectin-1. The enzyme sialylates N-glycans on CD45, a receptor tyrosine phosphatase that is a T cell receptor for galectin-1. ST6Gal I expression abrogates the reduction in CD45 tyrosine phosphatase activity that results from galectin1 binding. Sialylation of CD45 by the ST6Gal I also prevents galectin-1-induced clustering of CD45 on the T cell surface, an initial step in galectin-1 cell death. Regulation of glycoprotein sialylation may control susceptibility to cell death at specific points during T cell development and peripheral activation
-
-
-
additional information
?
-
-
the enzyme functions in Golgi vesicles to terminally sialylate the N-linked oligosaccharides of glycoproteins
-
-
-
additional information
?
-
-
the enzyme is responsible for the synthesis of 6'-sialylolifosaccharides in milk, ST6Gal II shows no activity towards oligosaccharides such as Galbeta1,3GalNAc, Galbeta1,3GlcNAc, lactose, and lacto-N-tetraose, which do not have the Galbeta1,4GlcNAc structure at the nonreducing end of their carbohydrate groups. Relatively low activity towards some glycoproteins, which are considered to have the Galbeta1,4GlcNAc structure at the nonreducing end of the carbohydrate groups
-
-
-
additional information
?
-
-
radiation exposure is found to increase the sialylation of glycoproteins such as integrin beta1 by inducing the expression of ST6Gal I, and increased protein sialylation contributed to cellular radiation resistance
-
-
-
additional information
?
-
-
soluble ST6Gal I produced by BACE1 plays, at least in part, a role in the sialylation of soluble glycoproteins
-
-
-
additional information
?
-
-
ST6Gal I sialyltransferase knock-out mouse lacks the main enzyme necessary for the attachment of alpha2,6 sialic acid to N-linked glycoproteins on the cell surface. Even in the absence of detectable alpha2,6 sialic acid in the mouse respiratory tract, human influenza viruses can still infect these mice and grow to similar titers in the lung and trachea as compared to wild-type animals. The presence of a major alpha2,6 sialic acid on N-linked glycoproteins is not essential for human influenza virus infection in mice
-
-
-
additional information
?
-
-
no sialidase activity is detected for the alpha2,6-sialyltransferase
-
-
-
additional information
?
-
-
the enzyme transfers Neu5Ac of CMP-Neu5Ac to the 6-hydroxyl group on internal GalNAc on O-glycans with strict specificities towards the O-glycan GalNAcalpha- (Tnantigen), Galbeta1-3GalNAcalpha- (T-antigen) and Neu5Acalpha2-3Galbeta1-3GalNAcalpha- (sialyl T-antigen) structures. hST6GalNAc-I shows activities towards O-linked oligosaccharides attached to peptides of glycoproteins and weak activities towards synthetic non-amino acid aglycons of O-glycans hST6GalNAc-I. hST6GalNAc-I shows strong activities with core 2 glycans (Galbeta1-3GalNAcbeta[GlcNAcbeta1-6]GalNAc). Core 2 O-glycan structures lacking the terminal galactose such as core 6 (GlcNAcbeta1-6GalNAcalpha-) or substituted with other monosaccharides such as core 4 (GlcNAcbeta1-3[GlcNAcbeta1-6]GalNAcalpha-) do not give any signal indicating that hST6GalNAc-I possibly also sialylated the terminal galactose of core 2 glycans. Relative specificities for the acceptors, Galbeta1-3GalNAcalphaOpNP (23%), Galbeta1-3[GlcNAcbeta1-6]GalNAcOalphapNP (12%), Galbeta1-3GalNAcOalphaThr (100%) and Galbeta1-3[GlcNAcbeta1-6]Galalpha2-6GalNAcOalphaThr (alpha2-6sialyl Tn-antigen). Relative specificities of the acceptors, Galbeta1-3GalNAcalphaOpNP (0%), Galbeta1-3[GlcNAcbeta1-6]GalNAcOalphapNP (0%), Galbeta1-3GalNAcOalphaThr (100%) and Galbeta1-3[GlcNAcbeta1-6]GalNAcalphaThr (19%) are found
-
-
-
additional information
?
-
-
DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid
-
-
-
additional information
?
-
-
human-like sialylated recombinant thyrotropin with sialic acid bound to galactose by both alpha2-3 and alpha2-6 linkages, similar to the native preparation, obtained in CHO cells modified to produce alpha2,6-sialyltransferase
-
-
-
additional information
?
-
-
neuraminidase activity is specific for the Neu5Acalpha2,6Gal linkage
-
-
-
additional information
?
-
A5D7T4
the enzyme uses the LacdiNAc structure (GalNAcbeta1,4GlcNAc) as a better acceptor substrate than the type II (Galbeta1-4GlcNAc) disaccharide. The enzyme's efficiency is improved when the acceptor substrate is provided as a free oligosaccharide rather than as a protein-bound oligosaccharide. No activity with alpha1-acidglycoprotein and Galbeta13GlcNAc-octyl
-
-
-
additional information
?
-
Q17WF8
no activity with Gal-beta1,3-GlcNAc, T-antigen Gal-beta1,3-GalNAc-alpha, and NeuAc-alpha2,3-Gal-beta1,4-Glc
-
-
-
additional information
?
-
-
the enzyme cannot transfer a N-acetylneuraminate residue to pyridylaminyl-LNFP I, a type 1 chain derivative of the blood group H antigen
-
-
-
additional information
?
-
-
the enzyme catalyzes alpha-2,6-sialylation of galactose on glycoproteins
-
-
-
additional information
?
-
-
the enzyme catalyzes the formation of the terminal alpha2,6-sialic acid linkages on gangliosides
-
-
-
additional information
?
-
Photobacterium leiognathi JT-SHIZ-119
-
neuraminidase activity is specific for the Neu5Acalpha2,6Gal linkage
-
-
-
additional information
?
-
Rattus norvegicus Sprague-Dawley
-
thyroglobin is no substrate, no activity with submaxillary asialo-mucin
-
-
-
additional information
?
-
Photobacterium damselae JT0160
-
low activity with several methylated substrates, no activity with UDP-Gal, UDP-GalNAc and UDP-Glc as donor substrates
-
-
-
additional information
?
-
Photobacterium damselae JT0160
-
enzyme does not recognize type I oligosaccharides
-
-
-
additional information
?
-
-
no sialidase activity is detected for the alpha2,6-sialyltransferase
-
-
-
additional information
?
-
Helicobacter acinonychis ATCC 51104
Q17WF8
no activity with Gal-beta1,3-GlcNAc, T-antigen Gal-beta1,3-GalNAc-alpha, and NeuAc-alpha2,3-Gal-beta1,4-Glc
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
R: glycoprotein or glycopeptide, one of a group of glycosyltransferases which act to assemble the carbohydrate units of thyroglobulin
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
regulation of enzyme expression
-
-
-
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
final step in synthesis of serum-type glycoproteins
-
-
-
CMP-N-acetylneuraminate + tumor necrosis factor receptor 1
CMP + alpha2,6-sialyated tumor necrosis factor receptor 1
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
addition of alpha2,6-linked sialic acid to the Gal1,4GlcNAc sequence, the preferred ligand for galectin-1, inhibits recognition of this saccharide ligand by galectin-1. The enzyme sialylates N-glycans on CD45, a receptor tyrosine phosphatase that is a T cell receptor for galectin-1. ST6Gal I expression abrogates the reduction in CD45 tyrosine phosphatase activity that results from galectin1 binding. Sialylation of CD45 by the ST6Gal I also prevents galectin-1-induced clustering of CD45 on the T cell surface, an initial step in galectin-1 cell death. Regulation of glycoprotein sialylation may control susceptibility to cell death at specific points during T cell development and peripheral activation
-
-
-
additional information
?
-
-
the enzyme functions in Golgi vesicles to terminally sialylate the N-linked oligosaccharides of glycoproteins
-
-
-
additional information
?
-
-
the enzyme is responsible for the synthesis of 6'-sialylolifosaccharides in milk
-
-
-
additional information
?
-
-
radiation exposure is found to increase the sialylation of glycoproteins such as integrin beta1 by inducing the expression of ST6Gal I, and increased protein sialylation contributed to cellular radiation resistance
-
-
-
additional information
?
-
-
soluble ST6Gal I produced by BACE1 plays, at least in part, a role in the sialylation of soluble glycoproteins
-
-
-
additional information
?
-
-
ST6Gal I sialyltransferase knock-out mouse lacks the main enzyme necessary for the attachment of alpha2,6 sialic acid to N-linked glycoproteins on the cell surface. Even in the absence of detectable alpha2,6 sialic acid in the mouse respiratory tract, human influenza viruses can still infect these mice and grow to similar titers in the lung and trachea as compared to wild-type animals. The presence of a major alpha2,6 sialic acid on N-linked glycoproteins is not essential for human influenza virus infection in mice
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Q17WF8
about 120% activity in the presence of 10 mM Mg2+
MgCl2
-
slight stimulation at 5 mM
MgCl2
-
no stimulation
MgCl2
-
no stimulation
NaCl
-
alpha2,6-sialyltransferase activity is increased approximately 40% in the presence of 300-800 mM
additional information
-
no metal ion requirement
additional information
-
no metal ion requirement
additional information
-
no metal ion requirement
additional information
-
no metal ion requirement
additional information
-
-
additional information
-
a metal ion is not required for the activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Aflatoxins
-
aflatoxins B1, B2a, G1, slightly by aflatoxins B2 and G2, inhibition is probably due to membrane disruption through binding of the aflatoxins and change in enzyme conformation
CDP
-
Mn2+ restores activity
CDP
-
competitive; wild-type enzyme and mutant V220A
CDP
-
at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST
CMP
-
competitive, less effective than CDP or CTP
CMP
-
is an efficient competitive inhibitor
CMP-4-O-[2,2,6,6-tetramethylpiperidine-1-oxyl]
-
-
CMP-4-O-[4-carboxy-2,2,6,6-tetramethylpiperidinine-1-oxyl]
-
-
CTP
-
also decreases the stimulation by the natural cytosolic rat colon factor
CTP
-
at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST
Cu2+
-
weak
cycloheximide
-
reduces the enzyme expression 3fold in normal hepatocytes and 16 to 17fold in Zaidela ascitic hepatoma, but not in cells isolated from the tumor and cultured for 48 h
deoxycholate
-
9fold stimulation at 0.25%, inactivation at 0.5%
EDTA
-
no inhibition
EDTA
-
weak
endo F
-
inactivation due to deglycosylation of the enzyme, best in methanol or ethanol, less efficient than glycanase
-
glycanase
-
inactivation due to deglycosylation of the enzyme, best in methanol or ethanol
-
long chain fatty acids
-
oleic acid, stearic acid, less efficient: palmitic acid, lauric acid, capric acid, caprylic acid or caproic acid, no inhibition by linoleic acid or linolenic acid
Mn2+
-
above 10 mM
N-acetyllactosamine
-
with asialo-alpha1 acid glycoprotein as substrate
PCMB
-
weak
UTP
-
0.25 mM, inhibition decreases at higher concentrations
Mn2+
Q17WF8
about 75% activity in the presence of 10 mM Mn2+
additional information
-
no inhibition by linoleic acid or linolenic acid
-
additional information
-
not affected by versenate
-
additional information
-
no inhibition by N-ethylmaleimide
-
additional information
-
-
-
additional information
-
inhibitory capacity of several synthetic trisaccharides
-
additional information
Q17WF8
not inhibited by EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
CMP
-
cytidine 5'-monophosphate, the product of sialyltransferase reactions, is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator. CMP can enhance the efficiency of the alpha2,6-sialidase activity of DELTA15Pd2,6ST in a dose-dependent manner
deoxycholate
-
9fold stimulation at 0.25%, inhibition at 0.5%
EDTA
-
activation
natural factor from rat colon
-
may also be present in rat brain and kidney; partially purified; specific, 4fold stimulation by decreasing the Km value for the substrates and increasing Vmax, present in the cytosol, temperature dependent, protease sensitive, independent of metal ions or detergent, unaffceted by monosaccharides; stimulation is decreased by CTP, no activation at 0.75 mM CTP
-
Triton CF-54/Tween 80
-
activation
-
Triton CF-54/Tween 80
-
-
-
Triton X-100
-
activation
Triton X-100
-
-
EDTA
-
no activation
additional information
-
in the sialyltransferase activity assay, FLAG-tagged ST6Gal1 has the highest expression level, which is 1.8- or 4.1fold higher than that of Strep- or His-tagged ST6Gal1, respectively
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.1
(2S,3S,4R,5R,6R)-5-Acetylamino-4-hydroxy-6-methoxy-3-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-2-carboxylic acid methyl ester
-
-
2.4
(2S,3S,4R,5R,6R)-5-Acetylamino-4-hydroxy-6-methoxy-3-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-2-carboxylic acid methylamide
-
-
11.2
(Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
-
pH 6.5, 37C
11.2
(Gal-3-O-Me)beta(1-3)(GlcNAc-6-O-Me)beta-O-Me
-
-
4.1
(Gal-3-O-Me)beta(1-3)GlcNAcbeta-O-Me
-
-
0.6
4-methylumbelliferyl beta-D-lactoside
-
pH 8.5, 37C, enzyme form DELTA112Pd2,6ST(N)
0.8
4-methylumbelliferyl beta-D-lactoside
-
pH 8.5, 37C, enzyme form DELTA15Pd2,6ST(N)
2.8
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
-
0.92
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
-
0.14
asialo-alpha1-acid glycoprotein
-
-
-
0.147
asialo-alpha1-acid glycoprotein
-
-
-
0.163
asialo-alpha1-acid glycoprotein
-
-
-
0.2
asialo-alpha1-acid glycoprotein
-
recombinant enzyme
-
0.23
asialo-alpha1-acid glycoprotein
-
with stimulation by the natural cytosolic crat colon factor
-
0.28
asialo-alpha1-acid glycoprotein
-
recombinant enzyme
-
0.33
asialo-alpha1-acid glycoprotein
-
wild-type enzyme
-
0.53
asialo-alpha1-acid glycoprotein
-
without stimulation by the natural cytosolic crat colon factor
-
0.078
asialo-ceruloplasmin
-
expressed as concentration of acceptor sites
-
0.0064
asialo-fetuin
-
expressed as concentration of terminal galactosyl residues
-
0.035
asialo-fetuin
-
-
-
0.59
asialo-thyroglobulin glycopeptides
-
-
-
0.16
asialo-transferrin
-
recombinant enzyme
-
0.21
asialo-transferrin
-
-
-
1.25
beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
-
0.051
CMP-9''-benzamido-Neu5Ac
-
pH 5.0, 37C
0.11
CMP-9''-benzamido-Neu5Ac
-
pH 5.0, 37C
0.05
CMP-9''-phenylacetamido-Neu5Ac
-
pH 5.0, 37C
0.34
CMP-9''-phenylacetamido-Neu5Ac
-
pH 5.0, 37C
0.098
CMP-9''-phenylhexanamido-Neu5Ac
-
pH 5.0, 37C
0.53
CMP-9''-phenylhexanamido-Neu5Ac
-
pH 5.0, 37C
0.11
CMP-9''-phenylpropionamido-Neu5Ac
-
pH 5.0, 37C
0.165
CMP-9''-phenylpropionamido-Neu5Ac
-
pH 5.0, 37C
0.12
CMP-9-acetamido-N-acetylneuraminate
-
-
0.72
CMP-9-amino-N-acetylneuraminate
-
-
0.03
CMP-9-benzamido-N-acetylneuraminate
-
-
0.003
CMP-N-acetylneuraminate
-
-
0.0053
CMP-N-acetylneuraminate
-
-
0.016
CMP-N-acetylneuraminate
-
-
0.0205
CMP-N-acetylneuraminate
-
-
0.035
CMP-N-acetylneuraminate
-
-
0.046
CMP-N-acetylneuraminate
-
-
0.047
CMP-N-acetylneuraminate
-
-
0.05
CMP-N-acetylneuraminate
-
with asialo-alpha1-acid glycoprotein
0.05
CMP-N-acetylneuraminate
-
wild-type enzyme; with asialo-alpha1-acid glycoprotein
0.05
CMP-N-acetylneuraminate
-
-
0.08
CMP-N-acetylneuraminate
-
with lactose
0.1
CMP-N-acetylneuraminate
-
apparent value, recombinant enzyme, using pyridylamine-type 2H as cosubstrate, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37C
0.11
CMP-N-acetylneuraminate
-
apparent value, recombinant enzyme, using pyridylaminyl-lacto-N-neotetraose as cosubstrate, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37C
0.14
CMP-N-acetylneuraminate
-
with stimulation by the natural cytosolic crat colon factor
0.15
CMP-N-acetylneuraminate
-
recombinant enzyme
0.24
CMP-N-acetylneuraminate
-
without stimulation by the natural cytosolic crat colon factor
0.27
CMP-N-acetylneuraminate
-
-
0.3
CMP-N-acetylneuraminate
-
with N-acetyllactosamine
0.32
CMP-N-acetylneuraminate
-
-
0.38
CMP-N-acetylneuraminate
-
-
0.555
CMP-N-acetylneuraminate
-
recombinant enzyme
0.9
CMP-N-acetylneuraminate
-
pH 8.5, 37C, enzyme form DELTA112Pd2,6ST(N); pH 8.5, 37C, enzyme form DELTA15Pd2,6ST(N)
0.3
CMP-Neu5Ac
-
alpha2,6-sialyltransferase activity of the truncated recombinant enzyme
0.12
cytidine-5'-monophospho-sialic acid
-
pH 5.0, 37C
0.14
cytidine-5'-monophospho-sialic acid
-
pH 5.0, 37C
3.3
Formic acid (2R,3R,4S,5R,6R)-2-((2R,3R,4R,5R,6R)-3-acetylamino-5-hydroxy-2-methoxy-6-methylaminomethyl-tetrahydro-pyran-4-yloxy)-4,5-dihydroxy-6-hydroxymethyl-tetrahydro-pyran-3-yl ester
-
pH 6.5, 37C
13.6
fucosyl-alpha-1,2-D-galactosyl-beta-1,4-D-glucose
-
-
2.04
Galbeta(1,4)GlcNAc-octyl
A5D7T4
soluble protein, at 37C
2.12
Galbeta(1,4)GlcNAc-octyl
A5D7T4
membrane-bound protein, at 37C
4.3
Galbeta(1-3)(GlcNAc-6-O-Me)beta-O-Me
-
-
1.7
Galbeta(1-3)GlcNAcbeta-O-Me
-
pH 6.5, 37C
1.7
Galbeta(1-3)GlcNAcbeta-O-Me
-
-
1.4
Galbeta(1-4)GlcNAc
-
non-reducing terminus of asialofetuin glycans
0.97
GalNAcbeta(1,4)GlcNAc-octyl
A5D7T4
soluble protein, at 37C
1.09
GalNAcbeta(1,4)GlcNAc-octyl
A5D7T4
membrane-bound protein, at 37C
1.67
lacto-N-neotetraose
-
-
1.8
lactose
-
pH 5.0, 37C, cosubstrate: CMP-9''-benzamido-Neu5Ac
2.5
lactose
-
pH 5.0, 37C, cosubstrate: CMP-9''-phenylacetamido-Neu5Ac; pH 5.0, 37C, cosubstrate: CMP-phenylhexanamido-Neu5Ac
2.6
lactose
-
pH 5.0, 37C, cosubstrate: CMP-phenylpropionamido-Neu5Ac
3.6
lactose
-
pH 5.0, 37C, cosubstrate: cytidine-5'-monophospho-sialic acid
6.82
lactose
-
-
17.2
lactose
-
alpha2,6-sialyltransferase activity of the truncated recombinant enzyme
31
lactose
-
-
129
lactose
-
-
0.7
Methanesulfonic acid (2R,3S,4R,5R,6R)-5-acetylamino-4-hydroxy-6-methoxy-3-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-2-ylmethyl ester
-
-
99
methyl-beta-D-galactopyranoside
-
-
11.6
N-((1R,3R,5R,6R,7S,8R,13R,15R,16R,17S)-6,7,17-Trihydroxy-5-hydroxymethyl-15-methoxy-2,4,9,11,14-pentaoxa-tricyclo[11.3.1.03,8]heptadec-16-yl)-acetamide
-
pH 6.5, 37C
1
N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18R)-6,7,18-trihydroxy-5-hydroxymethyl-16-methoxy-11-oxo-2,4,9,15-tetraoxa-12-aza-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
-
pH 6.5, 37C
1.1
N-((1R,3R,5R,6R,7S,8R,14R,16R,17R,18S)-6,7,18-Trihydroxy-5-hydroxymethyl-16-methoxy-2,4,9,12,15-pentaoxa-tricyclo[12.3.1.03,8]octadec-17-yl)-acetamide
-
pH 6.5, 37C
1.62
N-acetyllactosamine
-
-
2
N-acetyllactosamine
-
pH 5.0, 37C, cosubstrate: CMP-9''-phenylacetamido-Neu5Ac
2.3
N-acetyllactosamine
-
recombinant enzyme
4.3
N-acetyllactosamine
-
pH 5.0, 37C, cosubstrate: cytidine-5'-monophospho-sialic acid
5.2
N-acetyllactosamine
-
pH 5.0, 37C, cosubstrate: CMP-9''-phenylhexanamido-Neu5Ac
6.8
N-acetyllactosamine
-
pH 5.0, 37C, cosubstrate: CMP-9''-benzamido-Neu5Ac
10
N-acetyllactosamine
-
pH 5.0, 37C, cosubstrate: CMP-9''-phenylpropionamido-Neu5Ac
12
N-acetyllactosamine
-
-
15.4
N-acetyllactosamine
-
alpha2,6-sialyltransferase activity of the truncated recombinant enzyme
8.95
N-acetyllactosaminide
-
-
0.8
N-[(2R,3R,4R,5S,6R)-4-Hydroxy-6-(methanesulfonylamino-methyl)-2-methoxy-5-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-3-yl]-acetamide
-
-
3.1
N-[(2R,3R,4R,5S,6R)-5-((2S,3R,4S,5R,6R)-4,5-Dihydroxy-6-hydroxymethyl-3-methoxy-tetrahydro-pyran-2-yloxy)-6-formylaminomethyl-4-hydroxy-2-methoxy-tetrahydro-pyran-3-yl]-acetamide
-
-
1.4
N-[(2R,3R,4R,5S,6R)-6-(Acetylamino-methyl)-4-hydroxy-2-methoxy-5-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-3-yl]-acetamide
-
-
5.8
N-[(2R,3R,4R,5S,6R)-6-Aminomethyl-4-hydroxy-2-methoxy-5-((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-pyran-3-yl]-acetamide
-
-
8.1
Neu5Acalpha-2,3-Galbeta-1,4-Glc
-
-
53
Neu5Acalpha-4-nitrophenyl
-
alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST
0.91
nLc4Cer
-
recombinant enzyme
3.5
pyridylamine-type 2H
-
recombinant enzyme, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37C
102
pyridylamino-lactose
-
recombinant enzyme
1.3
pyridylamino-N-acetyllactosamine
-
recombinant enzyme
1.5
pyridylamino-nLc4Cer
-
recombinant enzyme
0.9
pyridylaminyl-lacto-N-neotetraose
-
recombinant enzyme, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37C
174
methyl-beta-D-galactopyranoside
-
-
additional information
additional information
-
kinetic data
-
additional information
additional information
-
kinetic data
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic mechanism
-
additional information
additional information
-
kinetics, several synthetic trisaccharides used as substrates
-
additional information
additional information
-
-
-
additional information
additional information
-
Km-values for synthetic CMP-N-acetylneuraminate analogues
-
additional information
additional information
-
several recombinant truncated mutants
-
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.1
4-methylumbelliferyl beta-D-lactoside
-
pH 8.5, 37C, enzyme form DELTA112Pd2,6ST(N)
2.3
4-methylumbelliferyl beta-D-lactoside
-
pH 8.5, 37C, enzyme form DELTA15Pd2,6ST(N)
0.087
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
-
1.13
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
-
1.4
CMP-N-acetylneuraminate
-
pH 8.5, 37C, enzyme form DELTA112Pd2,6ST(N)
2.7
CMP-N-acetylneuraminate
-
pH 8.5, 37C, enzyme form DELTA15Pd2,6ST(N)
0.883
Neu5Acalpha-4-nitrophenyl
-
alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.031
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
-
40078
1.23
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
-
40077
0.016
Neu5Acalpha-4-nitrophenyl
-
alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST
40079
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0047
CDP
-
wild-type enzyme
0.016
CDP
-
mutant V220A
0.18
CMP
-
-
4.07
CMP-4-O-[2,2,6,6-tetramethylpiperidine-1-oxyl]
-
pH 6.5, 37C
0.79
CMP-4-O-[4-carboxy-2,2,6,6-tetramethylpiperidinine-1-oxyl]
-
pH 6.5, 37C
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8.7
CMP-4-O-[2,2,6,6-tetramethylpiperidine-1-oxyl]
-
pH 6.5, 37C
1.7
CMP-4-O-[4-carboxy-2,2,6,6-tetramethylpiperidinine-1-oxyl]
-
pH 6.5, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00000022
-
hepatocyte
0.0000008
-
Ehrlich ascite carcinoma cells, adherent or from tissue culture
0.00083
-
partially purified enzyme
0.0009
-
partially purified enzyme
0.0035
-
partially purified enzyme
0.016
-
crude enzyme
0.103
-
neuraminidase activity of the truncated recombinant enzyme
1.6
-
purified, recombinant from Sf9 insect cells
2.6
-
159fold purified enzyme
5.5
-
purified enzyme
5.5
-
crude extract, alpha2,6-sialyltransferase activity of the truncated recombinant enzyme
8.2
-
purified enzyme
26 - 28
-
purified enzyme
82.9
-
alpha2,6-sialyltransferase activity of the 15.2fold purified truncated recombinant enzyme
additional information
-
kinetics
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
activity of stimulated and unstimulated enzyme with different substrates
additional information
-
assay method development, biosensor-based method using immobilized Sambucus nigra agglutinin
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 6
-
pH 5: alpha2,6-sialyltransferase activity of the truncated recombinant enzyme, pH 6: neuraminidase activity of the truncated recombinant enzyme
5.8
-
alpha2,6-sialidase and alpha2,6-trans-sialidase activities of DELTA15Pd2,6ST in MES buffer
6 - 6.5
-
2-(N-morpholino)ethane sulfonic acid buffer
6 - 6.5
-
-
6
-
broad
6
-
assay at
6
-
purified enzyme
6.4 - 7.2
-
broad, optimal buffer concentration: 0.05-0.15 M, decreasing activity above 0.15 M
6.5
-
assay at
6.5
-
assay at
6.8
-
assay at
7.5
-
Tris-HCl buffer
additional information
-
pI: 4.6
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 7.5
-
about 40% of maximal activity at pH 5.0 and at pH 7.5
5 - 9.2
-
about half-maximal activity at pH 5 and 9.2
5 - 9.7
-
about 65% of maximal activity at pH 5 and about half-maximal activity at pH 9.7
5.2 - 7.4
-
about half-maximal activity at pH 5.2 and 7.4
5.4 - 8
-
about half-maximal activity at pH 5.4 and 8, goat
7.5 - 10
-
pH-profile of truncated enzyme forms DELTA15Pd2,6ST(N) and DELTA112Pd2,6ST(N). Similar to the DELTA15Pd2,6ST(N), the shorter DELTA112Pd2,6ST(N) is active in a wide pH range of 7.5-10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
-
alpha2,6-sialyltransferase activity and neuraminidase activity of the truncated recombinant enzyme
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
purified enzyme
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 37
-
activity at 25C is about 75% of the activity at 100C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.3
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
level of serum ST6Gal I in patients with chronic active hepatitis is significantly increased compared to that in patients with chronic persistent hepatitis. Serum level of ST6Gal I in hepatitis C patients is correlated with the activity of hepatic inflammation, but not with liver fibrosis
Manually annotated by BRENDA team
-
of 11-, 15- and 17-day embryos
Manually annotated by BRENDA team
-
expressed during embryonic development in a tissue- and stage-specific fashion, with elevated expression in a subset of cells within the central nervous system
Manually annotated by BRENDA team
-
high activity in cells grown in tissue culture or adherent to extracellular matrices, low activity in cells not adherent nor grown in tissue culture
Manually annotated by BRENDA team
-
11-day, 15-day or 17-day, brain of 11-, 15- and 17-day old embryos
Manually annotated by BRENDA team
-
BACE1 affects the alpha2,6-sialylation of proteins secreted from Hep-3B cells. Following BACE1 overexpression in Hep3B cells, the alpha2,6-sialylation of soluble secreted glycoproteins is significantly increased, whereas that of cell-surface glycoproteins remains unchanged
Manually annotated by BRENDA team
-
cultured cells isolated from the tumor behave as normal hepatocytes, no enzyme activity in Zaidela ascitic hepatoma, but 3.6fold reduced mRNA content of a larger transcript size compared to normal liver cells
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
ST6GalI protein level is negligibly decreased in moderate drinkers but decreases by 30% in heavy alcohol drinkers compared to non-drinkers. The appearance of asialoconjugates in alcoholics is possibly due to the down-regulation of ST6GalI gene expression
Manually annotated by BRENDA team
-
from liver-injured model mice
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
from liver-injured model mice
-
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
A5D7T4
high level
Manually annotated by BRENDA team
A5D7T4
low level
Manually annotated by BRENDA team
-
of pregnant mouse
Manually annotated by BRENDA team
-
from heart and abdominal aorta
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
from heart and abdominal aorta
-
Manually annotated by BRENDA team
-
both mRNA expression and enzyme activity of ST6Gal I do not show a significant difference in the placental tissues of the women with preeclampsia and without preeclampsia. ST6Gal I is not involved in the pathogenesis of the preeclampsia
Manually annotated by BRENDA team
A5D7T4
low level
Manually annotated by BRENDA team
A5D7T4
very low level
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
-
very low expression
Manually annotated by BRENDA team
A5D7T4
very low level
Manually annotated by BRENDA team
-
myeloma cell line
Manually annotated by BRENDA team
additional information
-
ubiquitously expressed
Manually annotated by BRENDA team
additional information
A5D7T4
is absent from thymus and the adipose tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
A5D7T4
has a short cytosolic region of 11 amino acids (M1-G11)
Manually annotated by BRENDA team
-
isoform Stcys is found in both the endoplasmic reticulum and in Golgi
Manually annotated by BRENDA team
-
membrane-bound at the luminal side, trans compartment
Manually annotated by BRENDA team
-
the STtyr isoform is transiently localized in the Golgi and is ultimately cleaved and secreted, whereas the STcys isoform is stably localized in the Golgi and is not cleaved and secreted. Cytosolic tail and transmembrane region of the ST6Gal I both play roles in Golgi localization
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
exogenous and endogenous ST6Gal I in the perinuclear Golgi of Hep3B-ST6Gal I and control Hep3B cells
-
Manually annotated by BRENDA team
Q64685
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
-
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
Q701R1
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
-
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
Q683P0
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
-
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
Q9GU23
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
Q683P1
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
Q683N9
the enzyme is a Golgi type II transmembrane glycosyltransferase
Manually annotated by BRENDA team
-
in medial-trans regions. Isoform Stcys is found in both the endoplasmic reticulum and in Golgi, in medial-trans regions. Isoform STtyr is restricted to Golgi in most cells
-
Manually annotated by BRENDA team
A5D7T4
is a type II membrane-bound protein with a short cytosolic region of 11 amino acids (M1-G11), a single transmembrane region of 19 amino acids (M12-F30) and a luminal domain of 465 amino acids (T31-A495)
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
-
Manually annotated by BRENDA team
additional information
-
enzyme is not catalytically active in the endoplasmic reticulum and only achieves full activity when it locates itself to the trans compartment of the Golgi system
-
Manually annotated by BRENDA team
additional information
-
deletion of the transmembrane fragment results in loss of acceptor preference
-
Manually annotated by BRENDA team
additional information
-
oxidative stress changes the intracellular localizations of ST6Gal I and BACE1, more disperse localizations
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
42900
-
low molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one
637274
45000
-
low molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one
637274
57900
-
high molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one
637274
59000
-
gel filtration
684211
64000
-
gel filtration
637289
80000
-
high molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one
637274
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 50000, SDS-PAGE
?
F1SWB0
x * 45000, SDS-PAGE
?
-
x * 55000, SDS-PAGE
?
-
x * 60122, calculation from nucleotide sequence
?
-
x * 56732, MALDI-TOF
?
-
x * 55000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE, x * 57000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE
?
-
x * 55000, SDS-PAGE, x * 56732, MALDI-TOF
-
monomer
-
1 * 40000, SDS-PAGE
monomer
-
1 * 61000, SDS-PAGE
monomer
-
1 * 70000, SDS-PAGE
monomer
-
1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions
monomer
-
1 * 40500, SDS-PAGE under reducing conditions
monomer
-
1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions, 1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions, 1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions
oligomer
-
STcys oligomerization is a secondary event resulting from its concentration in the Golgi via mechanisms independently mediated by its cytosolic tail and transmembrane region
monomer
Photobacterium damselae JT0160
-
1 * 61000, SDS-PAGE
-
additional information
Q64685
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
-
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
Q701R1
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
-
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
Q683P0
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
-
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
Q9GU23
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
Q683P1
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
additional information
Q683N9
the enzymes contain four conserved peptide motifs: L/large, S/small, motif III, and motif VS/very small
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sialoprotein
-
recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward beta-linked GalNAc termini of its own N-linked glycans
glycoprotein
-
2times N-glycosylated
glycoprotein
-
the enzyme has two N-glycosylation sites
glycoprotein
-
contains at least 2 N-linked carbohydate chains of the complex type, catalytic activity of the enzyme depends on the presence of oligosaccharide chains
additional information
-
deglycosylation of the enzyme is much more efficient in presence of methanol or ethanol
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method at 20C. The crystal belongs to space group P3(1)2(1) or P3(2)2(1), with unit-cell parameters a = b = 90.29, c = 204.33 A. X-ray diffraction data are collected to 2.5 A resolution
-
single crystals are grown by the hanging-drop vapor diffusion method at 20C. Crystal structure of DELTA16psp26ST, the N-terminal truncated form of alpha2,6-sialyltransferase from Photobacterium sp. JT-ISH-224, complexed with a donor productCMPand an acceptor substrate lactose
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
-
purified enzyme is relatively stable over a wide range of pH
702879
5.2 - 5.5
-
most stable
637274
5.3
-
and below, storage stability decreases with t1/2: about 3 months
637270
6
-
full stability for at least 1 year
637270
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
-
purified enzyme is stable below 20C but is rapidly inactivated at temperatures above 20C
702879
40
-
5 min, 80% remaining activity
637289
55
-
5 min, 5% remaining activity
637289
67
-
30 s, 90% loss of activity
637269
100
-
5 min, complete inactivation
637268
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 50%, stabilizes during storage
-
high protein concentrations stabilize
-
stable to lyophilization
-
use of plastic containers instead of glass stabilizes
-
fractionation on Ultrogel AcA34 decreases activity, bovine serum albumin restores
-
freeze-thawing, crude preparation, stable to
-
glycerol, 20%, v/v, stabilizes
-
high NaCl concentrations stabilize during storage
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Methanol
-
stable at 10%, 37C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 50% glycerol, 12 mM cacodylate, pH 5.3, 2 months
-
0-4C, partially purified enzyme preparation, 2-4 weeks
-
frozen, crude, solubilized enzyme preparation, several months
-
0-4C, partially purified enzyme preparation, 2-4 weeks
-
-20C, crude enzyme preparation, at least 3 months
-
4C, partially purified enzyme preparation in 20% glycerol, v/v, at least 1 week
-
-80C, 20 mM sodium cacodylate, pH 5.0, stable at least 3 months
-
-20C, in 50% glycerol, 35 mM cacodylate, pH 6, 0.45 M NaCl, 0.08% Triton CF-54, at least 1 year
-
3C, microsomal fraction, 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CDP-hexanolamine-agarose affinity chromatography
-
partial
-
partial; solubilized by ultrasonic treatment
-
partial
-
Ni-Sepharose column chromatography
F1SWB0
amylose column chromatography
Q17WF8
partial, solubilized with Triton X-100
-
protein G Sepharose column chromatography
-
recombinant from cell culture medium after expression in insect cells
-
recombinant from COS-1 cells
-
IgG Sepharose column chromatography
-
truncated enzyme forms
-
N-terminal truncated form, by gel filtration, 15.2fold, with a yield of 53.2%
-
CDP-hexanolamine-agarose affinity chromatography
-
FLAG-tagged recombinant ST6Gal1 purified by precipitation with ammonium sulphate followed by affinity chromatography, with a recovery yield of 64%, 159fold
-
further purification of commercial rat liver enzyme
-
isoform STcys; isoform STtyr
-
soluble ST6Gal I-FLAG purified from conditioned media on affinity gel
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q683N9
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
A5D7T4, O18974
two pFlag-bST6Gal II vector constructions separately transfected in COS-1 cells for expression of either membrane-bound or soluble active forms of ST6Gal II
A5D7T4
expressed in Escherichia coli Rosetta (DE3)pLysS cells
F1SWB0
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q6KB61, Q701R2
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q9GU23
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q683P0
a full-length Drosophila SiaT protein with a C-terminal HA tag is transiently expressed in Drosophila S2 cells. A fraction of SiaT-HA protein expressed in S2 cell culture is secreted into the cell medium in soluble form
-
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q683P1
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q701R0, Q92182
expressed in Escherichia coli strain AD202
Q17WF8
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
P15907, Q96JF0
DNA sequence determination, expression of various truncated forms lacking the transmembrane fragment as FLAG-tagged proteins, the chimeric mutant, and the membrane-bound enzyme form in CHO-K1 cells; expression in Pichia pastoris, entry in the secretory pathway, i.e. excretion of the soluble enzyme to the medium, by usage of the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor, no hyperglycosylation
-
DNA sequence determination, functional overexpression of full length cDNA and fragment of residues 113-1227, the latter fused to staphylococcal protein A, in COS-1 cells
-
expressed in COS-1 cells
-
expressed in Nicotiana tabacum suspension-cultured BY-2 cells
-
expressed in U-373MG cells
-
expressed in Vero cells
-
expression in Pichia pastoris, entry in the secretory pathway, i.e. excretion of the soluble enzyme to the medium, by usage of the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor, no hyperglycosylation
-
functional expression in Saccharomyces cerevisiae
-
Hep3B cells stably expressing human BACE1-myc or ST6Gal I
-
high level functional expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection using a vector that fuses the enzyme to the mouse IgM signal peptide and the IgG binding domain of the Staphylococcus aureus protein A at the N-terminus for secretion of the recombinant protein into the culture medium
-
overexpression of ST6Gal I in SW480 colon cancer cells that initially show a low level of ST6Gal I expression increases the sialylation of integrin beta1 and also increases the stability of the protein
-
ST6Gal I FLAG-pcDNA construct transiently transfected into COS-7 cells
-
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q64685
expressed in COS-7 cells
-
expression in COS-7 cells
-
ST6Gal I FLAG-pcDNA construct transiently transfected into COS-7 cells
-
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q5K027, Q5K028
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q701R4, Q701R5
cloned as N- and C- His-tagged fusion proteins with different lengths (16-497 aa or 113-497 aa). Expression and activity assays indicate that the N-terminal 112 amino acid residues of the protein are not required for its alpha2,6-sialyltransferase activity but affect the solubility of the recombinant protein expressed in Escherichia coli
-
expressed in Escherichia coli
-
expression cassettes of the full-length form in pLST-FL and of the truncated form (N-terminal Lys2 to Ala15 deletion) in pLST-DELTAN expressed in Escherichia coli
-
expression in Escherichia coli
-
the putative mature form of JT-ISH-224 alpha2,6-sialyltransferase is overproduced in Escherichia coli
-
a CHO clone displaying a high rat alpha2,6-ST activity co-transfected with dicistronic mRNA expression vectors pEDdc-alpha-hTSH and pEAdc-beta-hTSH in a ratio of 1:5
-
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
P13721, Q701R3
enzyme expression analysis in Zaidela ascitic hepatoma cell lines and normal liver cells
-
expression in COS cells
-
expression of the ST6Gal I enzyme in a galectin-1-sensitive murine T cell line
-
expression of wild-type and mutants in COS-1 cells
-
isoform STcys expressed in CHO cells or in HeLa cells; isoform STtyr expressed in CHO cells or in HeLa cells
-
ST6Gal I FLAG-pcDNA construct transiently transfected into COS-7 cells
-
ST6Gal1 cloned as N-terminal His-, Strep- and FLAG-tagged fusion proteins. FLAG-tagged recombinant ST6Gal1 expressed in fifth instar silkworm larval hemolymph using recombinant both cysteine protease- and chitinase-deficient Bombyx mori nucleopolyhedrovirus (BmNPV-CP--Chi-) bacmid
-
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview; DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q5QQ37, Q6KB62
DNA and amino acid sequence analysis, detailed phylogenetic analysis of the ST6GalNAc family and the four subfamilies, sialyltransferases show a common genetic origin, by successive duplications of an ancestral gene, followed by divergent evolution, overview
Q701R1
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ST6Gal II gene is upregulated in cells stimulated by interleukin-6, whereas interleukin-1 and TNFalpha treatments have no effect on the expression of ST6Gal II. Inflammatory response induced by interleukin-6 treatment provokes an overexpression of ST6Gal II at 24 h and 48 h of treatment, its expression level in the treated cells is 2.5- and 5.6fold higher than in the control, respectively
A5D7T4
enzyme activity has little variation in dogs with metastatic tumors
-
expression of ST6GALNAC2 in B peripheral lymphocytes is significantly lower in patients with IgA nephropathy than that in normal controls
-
alpha2,6-ST activity in B lymphocytes is correlated positively with the level of alpha2,6-sialic acid in serum IgA1 in patients and controls
-
the enzyme is up-regulated in human colon cancer
-
carbon tetrachloride administration significantly increases the level of plasma ST6Gal I as evidenced from significant increases in plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as hepatitis markers
-
carbon tetrachloride administration significantly increases the level of plasma ST6Gal I as evidenced from significant increases in plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as hepatitis markers
Mus musculus C57BL/6
-
-
level of plasma ST6Gal I is increased in two different types of liver injury models: in zone 1 hepatocyte-injured rats, the level of plasma ST6Gal I is increased together with acute phase reactions. Meanwhile, in zone 3 hepatocyte-injured rats, ST6Gal I secretion is most likely triggered by oxidative stress
P13721
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C139A
-
the mutant has almost the same activity (98%) and specificity as those of native enzyme
C139S
-
the mutant has almost the same activity (94%) and specificity as those of native enzyme
C181S
-
no activity
C350A
-
no activity
D219A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 10.8fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
G221A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 1.6fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
K223A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 6.6fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
L190A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 11.9fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
T224A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 1.3fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
T225A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 3.2fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
V184A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 6.8fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
V220A
-
site-directed mutagenesis, mutant of the conserved sialyl-motif contained by all members of the sialyltransferase family, 6.0fold increased Km for CMP-NeuAc, only slightly altered Km for the acceptor substrate
additional information
-
construction of a chimeric enzyme with the N-terminal membrane portion of the rat core 2 beta1,6-GlcNAc-transferase, amino acid residues 1-52, and the residues 1-70 of the human alpha2,6-sialyltransferase, also FLAG-tagged, functional expression in CHO-K1 cells, broader acceptor substrate specificity than the wild-type
C350S
-
no activity
additional information
-
cloned as N- and C-His-tagged fusion proteins with different lengths (16-497 aa or 113-497 aa). Expression and activity assays indicate that the N-terminal 112 amino acid residues of the protein are not required for its alpha2,6-sialyltransferase activity. Among four truncated forms tested, N-His-tagged ELTA15Pd2,6ST(N) containing 16-497 amino acid residues has the highest expression level. Similar to the DELTA15Pd2,6ST(N), the shorter DELTA112Pd2,6ST(N) is active in a wide pH range of 7.5-10.0
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
-
recombinant enzyme may be used for in vitro synthesis of oligosaccharides
medicine
-
protein sialylation might be a target to overcome radioresistance in radiation therapie
medicine
-
two kinds of sandwich enzyme-linked immunosorbent assay (ELISA) systems are very useful tools for measuring plasma ST6Gal I, which represents a potential biomarker for diagnosing hepatic diseases
synthesis
-
recombinant enzyme may be used for in vitro synthesis of oligosaccharides
synthesis
-
enzyme can be exploited as biocatalyst in the synthesis of interesting non-natural compounds, especially in view of chemical, regiospecific sialylation, chemo-enzymatic synthesis of modified carbohydrate ligands, and their suitability as probes for studying molecular recognition phenomena
medicine
-
two kinds of sandwich enzyme-linked immunosorbent assay (ELISA) systems are very useful tools for measuring plasma ST6Gal I, which represents a potential biomarker for diagnosing hepatic diseases
medicine
Mus musculus C57BL/6
-
two kinds of sandwich enzyme-linked immunosorbent assay (ELISA) systems are very useful tools for measuring plasma ST6Gal I, which represents a potential biomarker for diagnosing hepatic diseases
-
synthesis
-
application of the alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST in the synthesis of sialosides
synthesis
-
because this enzyme is most active at basic pH, sialyltransferase obtained from Photobacterium leiognathi JT-SHIZ-145 is a promising tool for the efficient production of sialosides and the modification of glycoconjugates
synthesis
-
the enzyme may be a powerful tool for the synthesis of sialosides and the modification of sialyl-glycoconjugates
synthesis
Photobacterium leiognathi JT-SHIZ-119
-
the enzyme may be a powerful tool for the synthesis of sialosides and the modification of sialyl-glycoconjugates
-
synthesis
Photobacterium leiognathi JT-SHIZ-145
-
because this enzyme is most active at basic pH, sialyltransferase obtained from Photobacterium leiognathi JT-SHIZ-145 is a promising tool for the efficient production of sialosides and the modification of glycoconjugates
-
synthesis
-
the Photobacterium sialyltransferase can be used in the synthesis of sialoside analogues having a large substituent at the 9-position of Neu5Ac
medicine
-
two kinds of sandwich enzyme-linked immunosorbent assay (ELISA) systems are very useful tools for measuring plasma ST6Gal I, which represents a potential biomarker for diagnosing hepatic diseases
synthesis
-
enzyme can be exploited as biocatalyst in the synthesis of interesting non-natural compounds, especially in view of chemical, regiospecific sialylation, chemo-enzymatic synthesis of modified carbohydrate ligands, and their suitability as probes for studying molecular recognition phenomena