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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
guanosine phosphorylase,
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phosphorylase, guanosine
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guanosine + phosphate = guanine + alpha-D-ribose 1-phosphate
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pentosyl group transfer
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guanosine:phosphate alpha-D-ribosyltransferase
Also acts on deoxyguanosine.
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deoxyguanosine + phosphate
guanine + deoxyribose 1-phosphate
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guanosine + phosphate
guanine + D-ribose 1-phosphate
guanosine + phosphate
guanine + D-ribose 1-phosphate
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equilibrium constant is 0.019
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guanosine + phosphate
guanine + D-ribose 1-phosphate
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enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood
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guanosine + phosphate
guanine + D-ribose 1-phosphate
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guanosine + phosphate
guanine + D-ribose 1-phosphate
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enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood
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?
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PCMB
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0.2 mM, complete inhibition
Tris buffer
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0.1 M, pH 7.5, 22% inhibition
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2-mercaptoethanol
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required for optimal activity
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0.376
phosphate
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reaction with guanosine
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7
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phosphorolysis of deoxyguanosine
7 - 7.4
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phosphorolysis of guanosine
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5 - 8.5
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pH 5.0: about 35% of activity maximum, pH 8.5: about 45% of activity maximum
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brenda
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brenda
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brenda
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brenda
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brenda
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-10 C, stable for 1 month
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Yamada, E.W.
The phosphorolysis of nucleosides by rabbit bone marrow
J. Biol. Chem.
236
3043-3046
1961
Oryctolagus cuniculus
brenda
Heyworth, P.G.; Gutteridge, W.E.; Ginger, C.D.
Purine metabolism in Trichomonas vaginalis
FEBS Lett.
141
106-110
1982
Trichomonas vaginalis
brenda
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