Information on EC 2.4.2.1 - purine-nucleoside phosphorylase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota, dsDNA viruses, no RNA stage

EC NUMBER
COMMENTARY
2.4.2.1
-
RECOMMENDED NAME
GeneOntology No.
purine-nucleoside phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purine deoxyribonucleoside + phosphate = purine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
(2)
-
-
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
(1)
-
-
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
sequential mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
rapid equilibrium bi bi reaction
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
Theorell-Chance mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
ordered bi-bi mechanism with nucleoside being the first substrate to add and base product to leave
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
rapid equilibrium random bi-bi mechanism with formation of abortive complexes
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
Theorell-Chance mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
sequential bireactant mechanism
-
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
mechanism: 1. random binding of substrates, 2. potent binding and slow release of some reaction products leading to the circumstances that the chamical step is not the slowest one and that rapid-equilibrium assumptions do not hold, 3. dual role of phosphate, as substrate and as reaction modifier
P81989
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
show the reaction diagram
steady-state ordered bi bi kinetic mechanism, in which phosphate binds first followed by 2'-deoxyguanosine, and ribose 1-phosphate dissociates first followed by guanine. A general acid is essential for both catalysis and 2'-deoxyguanosine binding, and deprotonation of a group abolishes phosphate binding. Product release contributes to the rate-limiting step
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
adenine and adenosine salvage I
-
adenine and adenosine salvage III
-
adenine and adenosine salvage V
-
adenosine nucleotides degradation II
-
arsenate detoxification I (glutaredoxin)
-
Biosynthesis of secondary metabolites
-
fluoroacetate and fluorothreonine biosynthesis
-
guanine and guanosine salvage I
-
guanosine nucleotides degradation III
-
Metabolic pathways
-
Nicotinate and nicotinamide metabolism
-
purine deoxyribonucleosides degradation
-
Purine metabolism
-
purine ribonucleosides degradation
-
Pyrimidine metabolism
-
salinosporamide A biosynthesis
-
urate biosynthesis/inosine 5'-phosphate degradation
-
xanthine and xanthosine salvage
-
SYSTEMATIC NAME
IUBMB Comments
purine-nucleoside:phosphate ribosyltransferase
Specificity not completely determined. Can also catalyse ribosyltransferase reactions of the type catalysed by EC 2.4.2.5, nucleoside ribosyltransferase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
adenosine phosphorylase
-
-
-
-
bovPNP
-
-
bPNO
P55859
-
DeoD
O34925
gene name
DeoD
Bacillus subtilis 168
O34925
gene name
-
DeoD
P0ABP8
gene name
hPNP
-
-
hPNP
P00491
-
HsPNO
P00491
-
HsPNP
-
-
HsPNP
P00491
-
inosine phosphorylase
-
-
-
-
inosine-guanosine phosphorylase
-
-
-
-
nucleotide phosphatase (2.4.2.1)
-
-
-
-
PfPNP
Q8T9Z7
-
PfPNP
-, Q8U2I1
-
phosphorylase, purine nucleoside
-
-
-
-
PNP
Q2HXR2
-
PNP
Q2HXR2
-
-
PNPase
-
-
-
-
Pu-NPase
-
-
-
-
PUNP
-
-
-
-
PUNPI
-
-
-
-
PUNPII
-
-
-
-
purine deoxynucleoside phosphorylase
-
-
-
-
purine deoxyribonucleoside phosphorylase
-
-
-
-
purine nucleoside phosphorylase
-
-
-
-
purine nucleoside phosphorylase
-
-
purine nucleoside phosphorylase
B0LFE7
-
purine nucleoside phosphorylase
-
-
purine nucleoside phosphorylase
-
-
purine nucleoside phosphorylase DeoD
-
-
purine nucleoside phosphorylase II
-
-
purine ribonucleoside phosphorylase
-
-
-
-
SalT
B0LFE7
gene name
TgPNP
Q2HXR2
-
CAS REGISTRY NUMBER
COMMENTARY
9030-21-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Alcaligenes faecalis KY3106
KY3106
-
-
Manually annotated by BRENDA team
Arthrobacter nucleogenes
KY3168
-
-
Manually annotated by BRENDA team
Arthrobacter nucleogenes KY3168
KY3168
-
-
Manually annotated by BRENDA team
strain Alk36
UniProt
Manually annotated by BRENDA team
Bacillus halodurans Alk36
strain Alk36
UniProt
Manually annotated by BRENDA team
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-
-
Manually annotated by BRENDA team
Bacillus subtilis 168
-
UniProt
Manually annotated by BRENDA team
Bacillus subtilis 168
-
-
-
Manually annotated by BRENDA team
Bacterium cadaveris
-
-
-
Manually annotated by BRENDA team
Bacterium cadaveris
KY3402
-
-
Manually annotated by BRENDA team
Bacterium cadaveris KY3402
KY3402
-
-
Manually annotated by BRENDA team
calf
SwissProt
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
Cellulomonas cellasea KY3491
KY3491
-
-
Manually annotated by BRENDA team
Corynebacterium vitaeruminis KY3459
KY3459
-
-
Manually annotated by BRENDA team
Enterobacter cloacae KY3074
KY3074
-
-
Manually annotated by BRENDA team
; mutant enzyme M64V
-
-
Manually annotated by BRENDA team
K-12, inosine-guanosine phosphorylase
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus JTS 859
JTS 859
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus TH 6-2
TH 6-2
-
-
Manually annotated by BRENDA team
recombinant
SwissProt
Manually annotated by BRENDA team
Leucisus rusticus
-
-
-
Manually annotated by BRENDA team
Micrococcus luteus KY 3760
KY 3760
-
-
Manually annotated by BRENDA team
Pectobacterium carotovorum AJ 2992
AJ 2992
-
-
Manually annotated by BRENDA team
Proteus mirabilis KY4057
KY4057
-
-
Manually annotated by BRENDA team
Bsi590
A3F8V1
Uniprot
Manually annotated by BRENDA team
Pseudoalteromonas sp. Bsi590
Bsi590
A3F8V1
Uniprot
Manually annotated by BRENDA team
adenine phosphorylase activity and a distinct inosine-guanosine phosphorylase activity
-
-
Manually annotated by BRENDA team
gene salT
UniProt
Manually annotated by BRENDA team
salmon
-
-
-
Manually annotated by BRENDA team
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
LT-2
-
-
Manually annotated by BRENDA team
-
Q2HXR2
SwissProt
Manually annotated by BRENDA team
strain ME49
Q2HXR2
SwissProt
Manually annotated by BRENDA team
strain ME49
Q2HXR2
SwissProt
Manually annotated by BRENDA team
Xanthomonas campestris KY4208
KY4208
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
B0LFE7
the salT-deficient mutant strain shows reduced salinosporamide A and salinosporamide B synthesis
metabolism
B0LFE7
the enzyme is involved in the chloroethylmalonyl-CoA biosynthetic pathway, overview
physiological function
-
part of 4-fluorothreonine biosynthesis
physiological function
Q8T9Z7
targeted gene disruption results in loss of enzyme activity in lysate, with normal activity of adenosine deaminase. Mutant parasites show a greater requirement for exogenous purines and a severe growth defect at physiological concentrations of hypoxanthine. The mutant parasites are more sensitive to purine nucleoside phosphorylase inhibitors that bind human purine nucelotide phosphorylase tighter and less sensitive to inhibitor 5'-methylthio-immucillin-H
physiological function
-
parasites lacking purine nucelotide phosphorylase due to gene disruption are attenuated and clear in mice. Although able to form gametocytes, purine nucelotide phosphorylase-deficient parasites do not form oocysts in mosquito midguts and are not transmitted from mosquitoes to mice. Mice given purine nucelotide phosphorylase-deficient parasites are immune to subsequent challenge to a lethal inoculum of Plasmodium yoelii YM and to challenge from strain Plasmodium yoelii 17XNL
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,6-dihydropurine riboside + phosphate
alpha-D-ribose 1-phosphate + 1,6-dihydropurine
show the reaction diagram
-
-
-
-
?
1-methylguanosine + phosphate
alpha-D-ribose 1-phosphate + 1-methylguanine
show the reaction diagram
-
-
-
-
?
1-methylinosine + phosphate
alpha-D-ribose 1-phosphate + 1-methylhypoxanthine
show the reaction diagram
-
-
-
-
?
2',3'-dideoxyinosine + phosphate
hypoxanthine + alpha-D-2,3-dideoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2',3'-dideoxyinosine + phosphate
alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
show the reaction diagram
-
22% of the activity with inosine
-
-
?
2',3'-dideoxyinosine + phosphate
alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
show the reaction diagram
-
12% of the activity with inosine
-
-
?
2',3'-dideoxyinosine + phosphate
hypoxanthine + 2',3'-dideoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxy-6-methylpurine nucleoside + phosphate
?
show the reaction diagram
A3F8V1, -
13.2% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
12% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
A3F8V1, -
13.4% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
54.7% of the activity with 2'-deoxyinosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus subtilis, Bacillus subtilis 168
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
2-deoxy-alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
10% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
A4Q998, -
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
Q72IR2
-
22.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
A3F8V1, -
3.7% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
20% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
83.2% of the activity with 2'-deoxyinosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus subtilis, Bacillus subtilis 168
-
isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + alpha-D-2-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
show the reaction diagram
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
show the reaction diagram
A4Q998, -
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
show the reaction diagram
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
show the reaction diagram
-
-
-
-
?
2'-deoxyinosine + phosphate
2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine
show the reaction diagram
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
Q72IR2
-
40.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
22% of the activity with inosine
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
isoform of 816 amino acids, 23% of the activity with inosine, isoform of 702 amino acids, 31% of the activity with inosine
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
show the reaction diagram
-
-
-
-
-
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
show the reaction diagram
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
show the reaction diagram
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
show the reaction diagram
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + ?
show the reaction diagram
-
-
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
the product 2-fluoroadenine is highly cytotoxic for Trichomonas vaginalis
-
?
2-fluoro-2'-deoxyadenosine + phosphate
?
show the reaction diagram
-
a toxic prodrug
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
2-fluoroadenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no substrate for wild-type, substrate of double mutant E201Q/N243D
-
-
?
2-fluoroadenosine + phosphate
2-fluoroadenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
3-(beta-D-ribofuranosyl)adenine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
-
3-(beta-D-ribofuranosyl)adenine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
3-(beta-D-ribofuranosyl)adenine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
-
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
3-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
3-deazainosine + phosphate
alpha-D-ribose 1-phosphate + 3-deazahypoxanthine
show the reaction diagram
-
-
-
-
?
5'-chloro-5'-deoxy-adenosine + phosphate
5-chloro-5-deoxy-ribose 1-phosphate + adenine
show the reaction diagram
B0LFE7
-
-
-
-
5'-deoxy-5'-fluoroadenosine + phosphate
5-deoxy-5-fluoro-D-ribose-1-phosphate + adenine
show the reaction diagram
-
5'-deoxy-5'-fluoroadenosine + phosphate i.e. 5'-FDA
5-deoxy-5-fluoro-D-ribose-1-phosphate i.e. 5-FDRP
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
Q2HXR2
very low activity
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
-
ver low activity
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
show the reaction diagram
Q8T9Z7
-
-
-
?
6-(2-thienyl)-9-(beta-D-ribofuranosyl)purine + phosphate
6-(2-thienyl)purine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
6-cyclopropyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-cyclopropylpurine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
6-ethyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-ethylpurine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
6-mercaptoguanosine + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptoguanine
show the reaction diagram
-
-
-
-
?
6-mercaptopurine riboside + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptopurine
show the reaction diagram
-
-
-
-
-
6-mercaptopurine riboside + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptopurine
show the reaction diagram
-
14% of the activity with inosine
-
-
?
6-phenyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-phenylpurine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
6-thioxanthine + alpha-D-ribose 1-phosphate
6-thioxanthosine + phosphate
show the reaction diagram
-
-
-
-
?
7,8-dimethylguanosine + phosphate
7,8-dimethylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-(beta-D-ribofuranosyl)guanine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
7-(beta-D-ribofuranosyl)guanine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
P00491
-
-
-
?
7-(beta-D-ribofuranosyl)guanine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
P00491
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
show the reaction diagram
-
-
-
-
?
7-butylguanosine + phosphate
7-butylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-ethylguanosine + phosphate
7-ethylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-isobutylguanosine + phosphate
7-isobutylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-isopropylguanosine + phosphate
7-isopropylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methyl-6-thio-guanosine + phosphate
7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methyl-8-aminoguanosine + phosphate
7-methyl-8-aminoguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
P81989
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
7-N-methyl-6-thiopurine riboside + phosphate
?
show the reaction diagram
-
-
-
-
?
7-prolylguanosine + phosphate
7-prolylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
show the reaction diagram
-
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
show the reaction diagram
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
show the reaction diagram
P00491
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
show the reaction diagram
-
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
show the reaction diagram
-
-
-
-
-
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
show the reaction diagram
-
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
show the reaction diagram
-
-
-
-
r
8-azahypoxanthine + alpha-D-ribose 1-phosphate
8-azainosine + phosphate
show the reaction diagram
-
-
-
?
8-methylguanosine + phosphate
8-methylguanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine + phosphate
6-methylthiopurine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
9-beta-D-ribofuranosyl-6-methylthiopurine + phosphate
6-methylthiopurine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate
?
show the reaction diagram
-
a toxic prodrug
-
-
?
9-[6-deoxy-alpha-L-talofuranosyl]-2-F-adenine + phosphate
?
show the reaction diagram
-
-
-
-
?
9-[6-deoxy-alpha-L-talofuranosyl]-6-methylpurine + phosphate
?
show the reaction diagram
-
-
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity, a toxic prodrug, substrate of enzyme mutant M64V
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
show the reaction diagram
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity, a toxic prodrug, substrate of enzyme mutant M64V, liberation of methylpurine
-
-
?
9-[alpha-L-lyxofuranosyl]-2-F-adenine + phosphate
?
show the reaction diagram
-
-
-
-
?
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity, a toxic prodrug, substrate of enzyme mutant M64V
-
-
?
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
-
-
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
-
r
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
-
-
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
-
r
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
show the reaction diagram
P00491
-
-
-
r
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
-
r
adenine arabinoside + phosphate
alpha-D-arabinose 1-phosphate + adenine
show the reaction diagram
-
49% of the activity with adenosine
-
-
-
adenosine + arsenate
adenine + alpha-D-ribose 1-arsenate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
P46354
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q9BMI9
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no activity
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
best substrate
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
78% of the activity with deoxyguanosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
61% of the activity with deoxyguanosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
wild-type enzyme and mutant enzyme K244Q show no activity with adenosine, mutant enzyme N243D and N243D/K244Q are active with adenosine
-
-
-
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
in the reverse reaction the catalytic activity with adenine is higher than that with either hypoxanthine or guanine
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
160% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
activity with OUNOII, no activity with PUNPI
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
A3F8V1, -
15.4% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
41% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
45% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
59.9% of the activity with 2'-deoxyinosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus subtilis, Bacillus subtilis 168
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
160% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Pectobacterium carotovorum AJ 2992
-
48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Enterobacter aerogenes AJ 11125
-
best substrate
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus TH 6-2
-
no activity
-
-
-
adenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
P45563
activity with mutant enzyme N239D, wild-type and mutant enzyme Y191L show no activity
-
-
?
arabinofuranosyladenine + phosphate
?
show the reaction diagram
A3F8V1, -
4.3% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
P46354
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
78% of the activity with deoxyguanosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
61% of the activity with deoxyguanosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
112.9% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
2'-deoxyadenosine, 57% of the activity with inosine. 3'-deoxyadenosine, 7% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
-
2'-deoxyadenosine, 160% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
Enterobacter cloacae KY3074
-
112.9% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
show the reaction diagram
Enterobacter aerogenes AJ 11125
-
2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
P46354
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Q05788
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
-
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
-
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
2'-deoxyguanosine, 469% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
reaction with ionosine-guanosine phosphorylase, very low activity with the adenosine-specific phosphorylase
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
11% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
85% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
2'-deoxyguanosine, 469% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Pectobacterium carotovorum AJ 2992
-
2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus TH 6-2
-
85% of the activity with inosine
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
-
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
P46354
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
P00491
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Q05788
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Q2HXR2
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
as active as inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
2'-deoxyinosine and 5'-deoxyinosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
88% of the activity with deoxyguanosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
2'-deoxyinosine, 43% of the activity with adenosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
136% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
2'-deoxyinosine, 87% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
-
2'-deoxyinosine, 691% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
2'-deoxyinosine, 691% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Pectobacterium carotovorum AJ 2992
-
136% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Enterobacter aerogenes AJ 11125
-
2'-deoxyinosine, 43% of the activity with adenosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus TH 6-2
-
as active as inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
14% of the activity with inosine, 19% of the activity with inosine
-
-
?
guanine + alpha-D-ribose 1-phosphate
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
guanine + ribose 1-phosphate
guanosine + phosphate
show the reaction diagram
-
-
-
r
guanosine + arsenate
guanine + alpha-D-ribose 1-arsenate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
P46354
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q05788
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
A4Q998, -
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-, Q8U2I1
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q2HXR2
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-, Q9KCN8
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q72IR2
-
8.7% phosphorolysis of substrate within 30 min
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no activity
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
53% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
81% of the activity with deoxyguanosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
84% of the activity with guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
21% of the activity with inosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
47.4% of the reaction with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
2.3% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
48% of the activity with deoxyguanosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
16% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
P45563
no activity with mutant enzyme N239D
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
insight into catalytically relevant correlated motions in human purine nucleoside phosphorylase
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
A3F8V1, -
55.8% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-, Q8U2I1
absolute specificity for inosine and guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
56% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
58.7% of the activity with 2'-deoxyinosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
66% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus subtilis, Bacillus subtilis 168
-
isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
53% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Pectobacterium carotovorum AJ 2992
-
2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine
-
-
-
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus halodurans Alk36
Q9KCN8
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Enterobacter cloacae KY3074
-
47.4% of the reaction with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus TH 6-2
-
84% of the activity with guanosine
-
-
?
hypoxanthine + alpha-D-deoxyribose 1-phosphate
deoxyinosine + phosphate
show the reaction diagram
-
-
-
-
r
hypoxanthine + alpha-D-ribose 1-phosphate
inosine + phosphate
show the reaction diagram
-
-
-
-
r
inosine + arsenate
hypoxanthine + ribose 1-arsenate
show the reaction diagram
-
-
-
-
?
inosine + arsenate
hypoxanthine + ribose 1-arsenate
show the reaction diagram
-
ribosyl group is nearly dissociated from the base prior to attack of the arsenate
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
P46354
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
P45563
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q05788
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
A4Q998, -
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
A3F8V1, -
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
P81989
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
P55859
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q8T9Z7
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-, Q8U2I1
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q2HXR2
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-, Q9KCN8
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q9BMI9
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
C8CPR9, -
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q8I3X4
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Q72IR2
-
19.1% phosphorolysis of substrate within 30 min
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
no activity
-
-
-
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
mechanism
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
34% of the activity with adenosine, equilibrium when 65% of inosine has been phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
82% of the activity with deoxyguanosine
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
reaction reaches an equilibrium when 21% of the inosine is phosphorolyzed
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
the equilibrium is reached when approximately 14% of inosine is phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
46% of the activity with deoxyguanosine
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
reaction with ionosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-, Q8U2I1
absolute specificity for inosine and guanosine
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
83% of the activity with 2'-deoxyinosine
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus subtilis 168
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
C8CPR9
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus JTS 859
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Pectobacterium carotovorum AJ 2992
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Bacillus halodurans Alk36
Q9KCN8
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Enterobacter cloacae KY3074
-
the equilibrium is reached when approximately 14% of inosine is phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Enterobacter aerogenes AJ 11125
-
34% of the activity with adenosine, equilibrium when 65% of inosine has been phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
Geobacillus stearothermophilus TH 6-2
-
-
-
-
-
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
-
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
more '
?
show the reaction diagram
-
catalysis involves protonation of the purine base at position N7 by residue Asp204, which is triggered by Arg217
-
-
-
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
show the reaction diagram
P00491
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-ribofuranoside + phosphate
?
show the reaction diagram
-
-
-
-
?
purine riboside + phosphate
alpha-D-ribose 1-phosphate + purine
show the reaction diagram
-
-
-
-
?
ribavirin + phosphate
alpha-D-ribose 1-phosphate + 1,2,4-triazole-3-carboxamide
show the reaction diagram
-
22% of the activity with inosine. Phosphorolysis reaches an equilibrium when 15% of ribavirin is phosphorolyzed
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
show the reaction diagram
Q8I3X4
-
-
-
?
uridine + phosphate
?
show the reaction diagram
-
-
-
-
?
xanthosine + arsenate
xanthine + alpha-D-ribose 1-arsenate
show the reaction diagram
-
-
-
-
-
xanthosine + arsenate
xanthine + alpha-D-ribose 1-arsenate
show the reaction diagram
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
-
-
-
r
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
-
-
-
r
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
reaction only at high concentrations
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
26% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
-
43.5% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
P45563
no activity with mutant enzymes Y191L and N239D
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
show the reaction diagram
Enterobacter cloacae KY3074
-
43.5% of the activity with inosine
-
-
?
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-
-
-
additional information
?
-
salmon, Leucisus rusticus
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
-
additional information
?
-
-
cellular function seems concerned primarily with nucleoside breakdown
-
-
-
additional information
?
-
-
children lacking PNP activity exhibit severe T cell immunodeficiency while maintaining normal or exaggerated B cell function. The PNP deficiency results in very low uric acid concentrations and high concentrations of the nucleoside substrate of PNP in plasma and urine
-
-
-
additional information
?
-
P00491
in intact cells, the enzyme functions in the direction of phosphorolysis, leading to degradation of purine nucleosides via coupling with guanase and xanthine oxidase. PNP deficiency is a rare disorder associated with an autosomal recessive form of cellular, but not humoral, immunodeficiency, and comprises about 4% of all cases of severe combined immunodeficiency. Mutations identified in cases of PNP deficiency
-
-
-
additional information
?
-
-
key enzyme in purine salvage pathway
-
-
-
additional information
?
-
P00491
enzyme plays a key role in the purine salvage pathway. PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function
-
-
-
additional information
?
-
A4Q998, -
no activity is detected for adenosine or 2'-deoxyadenosine
-
-
-
additional information
?
-
-
enzyme is able to reactivate scrambled RNaseA
-
-
-
additional information
?
-
-
enzyme is more specific to natural 6-oxopurine nucleosides and synthetic compounds, and does not catalyze the phosphorolysis of adenosine
-
-
-
additional information
?
-
-
enzyme shows a high tolerance to the steric and hydrophobic environment at the 6-position of the synthesized purine ribonucleosides
-
-
-
additional information
?
-
Q72IR2
no substrate: acyclovir, benzimidazole, 2,6-diaminopurine
-
-
-
additional information
?
-
-
no substrate: xanthosine, uridine, 2'-deoxythymidine, cytidine
-
-
-
additional information
?
-
C8CPR9, -
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
-
additional information
?
-
C8CPR9
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2-fluoro-2'-deoxyadenosine + phosphate
?
show the reaction diagram
-
a toxic prodrug
-
-
?
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate
?
show the reaction diagram
-
a toxic prodrug
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
show the reaction diagram
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
salmon, Leucisus rusticus
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
-
additional information
?
-
-
cellular function seems concerned primarily with nucleoside breakdown
-
-
-
additional information
?
-
-
children lacking PNP activity exhibit severe T cell immunodeficiency while maintaining normal or exaggerated B cell function. The PNP deficiency results in very low uric acid concentrations and high concentrations of the nucleoside substrate of PNP in plasma and urine
-
-
-
additional information
?
-
P00491
in intact cells, the enzyme functions in the direction of phosphorolysis, leading to degradation of purine nucleosides via coupling with guanase and xanthine oxidase. PNP deficiency is a rare disorder associated with an autosomal recessive form of cellular, but not humoral, immunodeficiency, and comprises about 4% of all cases of severe combined immunodeficiency. Mutations identified in cases of PNP deficiency
-
-
-
additional information
?
-
-
key enzyme in purine salvage pathway
-
-
-
additional information
?
-
P00491
enzyme plays a key role in the purine salvage pathway. PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
with (hypoxanthine-9-yl)methyl aglycone, i.e. Yokomatsu compound
-
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranyl)ethylphosphonic acid with (hypoxanthine-9-yl)methyl aglycone
-
multisubstrate analogue inhibitor
(3R,4R)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
-
(3S)-3-(2-amino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-(3-chlorophenyl)propanoic acid
-
IC50: 0.000036 mM
(3S,4R)-4-(guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylcarbonylphosphonic acid
-
competitive. For enzyme from cancer cell lines, Ki values are around 10 to 24 nM
(3S,4S)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
-
(S)-3-(guanin-9-yl)pyrrolidin-N-ylcarbonylphosphonic acid
-
competitive. For enzyme from cancer cell lines, Ki values widely vary from 16 to 100 nM
1,6-Dihydropurine riboside
-
-
1-((2-pyrrolidine-1-yl)ethyl)uracil
Q8I3X4
inhibits both enzymic activity and growth of Plasmodium falciparum
1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine
-
-
1-[4-(2,4-dimethylphenyl)-1,3-thiazol-2-yl]guanidine
-
0.1 mM , 79.5% inhibition
1-[4-(4-methylphenyl)-1,3-thiazol-2-yl]guanidine
-
0.1 mM , 70.6% inhibition
2'-deoxy-immucillin-G
Q8T9Z7
-
2'-deoxy-immucillin-H
Q8T9Z7
-
2,6-diamino-7-(5-mercapto-3,3-dimethylpentyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00005 mM
2,6-diamino-7-(cyclohexylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.0066 mM
2,8-diamino-1,7-dihydro-6H-purine-6-thione
-
IC50: 0.00849 mM
2,8-diamino-1,9-dihydro-6H-purin-6-one
-
IC50: 0.002299 mM
2,8-diamino-3-(2-thienylmethyl)-3,9-dihydro-6H-purin-6-one
-
IC50: 0.001758 mM
2,8-diamino-9-(1,3-thiazol-4-ylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.001043 mM
2,8-diamino-9-(2-furylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000092 mM; IC50: 0.00271 mM
2,8-diamino-9-(2-thienylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000497 mM
2,8-diamino-9-(4-fluorobenzyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.00417 mM
2,8-diamino-9-(pyridin-2-ylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.001971 mM
2,8-diamino-9-[(4-methyl-2-thienyl)methyl]-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000362 mM
2,8-diamino-9-[4-(1H-imidazol-1-yl)benzyl]-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000852 mM
2,8-diamino-9-[[1-(heptyloxy)-2-hydroxyethoxy]methyl]-1,9-dihydro-6H-purin-6-one
-
IC50: 0.00152 mM
2-(3,4-dichlorophenyl)-5-thioxo-5,6-dihydro-1H-pyrazolo[4,3-d]pyrimidine-3,7(2H,4H)-dione
-
IC50: 0.00703 mM
2-amino-1,5-dihydro-7-[[(2S)-2-(aminomethyl)-1-pyrrolidinyl]-methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 600 nM
2-amino-1,5-dihydro-7-[[(2S)-2-(hydroxymethyl)-1-pyrrolidinyl]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
acetic acid salt, IC50: 4.0 nM
2-amino-1,5-dihydro-7-[[[2-(hydroxy)ethyl]amino]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 20 nM
2-amino-6-chloro-7-deazapurine 2'-deoxyriboside
-
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
in absence of phosphate the enzyme catalyzes a slow hydrolysis, which is accompanied by inactivation of the enzyme
2-Amino-6-methylthiopurine
-
-
2-amino-6-oxo-9-[3-hydroxy-2-(phosphonomethoxy)propyl]-purine
P81989
-
2-amino-7-(1,2-dithian-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000018 mM
2-amino-7-(2,3,5-trichlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00024 mM
2-amino-7-(2-chlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00012 mM
2-amino-7-(2-furylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000083 mM
2-amino-7-(2-hydroxybenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00027 mM
2-amino-7-(2-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000021 mM
2-amino-7-(2-thienylmethyl)-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one
-
IC50: 0.000674 mM
2-amino-7-(3,4-dichlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000017 mM
2-amino-7-(3-chlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00002 mM
2-amino-7-(3-fluorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000024 mM
2-amino-7-(3-hydroxybenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00007 mM
2-amino-7-(3-methoxybenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000082 mM
2-amino-7-(3-methylbenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000057mM
2-amino-7-(3-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2-amino-7-(4-chlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2-amino-7-(4-iodobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000023 mM
2-amino-7-(biphenyl-4-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000546 mM
2-amino-7-(cycloheptylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00003 mM
2-amino-7-(cyclohexylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000047 mM
2-amino-7-(cyclopentylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000029 mM
2-amino-7-(piperidin-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.001 mM
2-amino-7-(pyridin-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000015 mM
2-amino-7-(pyridin-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
2-amino-7-(pyridin-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2-amino-7-(pyridin-4-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000064 mM
2-amino-7-(tetrahydro-2-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000011 mM
2-amino-7-(tetrahydrofuran-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00007 mM
2-amino-7-([[(2R,3S)-1,3,4-trihydroxybutan-2-yl]amino]methyl)-3,5-dihydro-4Hpyrrolo[3,2-d]pyrimidin-4-one
-
i.e. DATMe-immucillin-G
2-amino-7-benzyl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000051 mM
2-amino-7-cyclohex-1-en-1-yl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.0019 mM
2-amino-7-phenoxy-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000042 mM
2-amino-7-[(3-methylcyclohexyl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2-amino-7-[(3-tert-butylcyclohexyl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2-amino-7-[(4-chloropyridin-3-yl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000135 mM
2-amino-7-[3-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000124 mM; IC50: 0.000147 mM
2-amino-7-[3-(trifluoromethyl)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000036 mM
2-amino-7-[4-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
2-amino-7-[4-(propan-2-yl)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
1.8fold selectivity for Schistosoma enzyme over human enzyme
2-amino-7-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
i.e. SerMe-immucillin-G
2-amino-7-[[(1R,3S,5S,7S)-4-methyl-2-adamantyl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00009 mM
2-amino-7-[[(2-hydroxyethyl)(methyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 60 nM
2-amino-7-[[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 620 nM
2-amino-7-[[(2S)-2-(methoxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 600 nM
2-amino-7-[[(2S,4R)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 2 nM
2-amino-7-[[3-(hydroxymethyl)piperidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.004 mM
2-amino-7-[[bis(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 5 nM
2-amino-7-[[ethyl(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 220 nM
2-amino-9-(2-thienylmethyl)-8-thioxo-1,7,8,9-tetrahydro-6H-purin-6-one
-
IC50: 0.002866 mM
2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine
P81989
-
2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine
-
binding of multisubstrate analogue inhibitor to trimeric PNPs is a one-step process
2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine
-
kinetics of binding of multisubstrate analogue inhibitor; kinetics of binding of the multisubstrate analogue inhibitor 2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine with the trimeric purine nucleoside phosphorylase
2-chloro-6-(3-phenyl-1-propoxy)purine
-
-
2-chloroadenosine
-
inhibits ribosylation of adenine and N6-furfuryladenine
2-fluoro-2'-deoxyadenosine
-
the substrate releases highly cytotoxic 2-fluoroadenine. 2-Fluoro-2'-deoxyadenosine and 2-fluoroadenine exert strong inhibition of Trichomonas vaginalis growth with estimated IC50 values of 106 nM and 84 nM. 2-Fluoro-2'-deoxyadenosine might be useful as a potential chemotherapeutic agent against Trichomonas vaginalis
2-mercaptoethanol
-
-
2-phenyl-5,7-dithioxo-1,2,4,5,6,7-hexahydro-3H-pyrazolo[4,3-d]pyrimidin-3-one
-
IC50: 0.02284 mM
3,5-bis-(4-chlorobenzoyl)-alpha-D-ribose 1-phosphate
-
-
-
3,5-bis-(4-chlorobenzoyl)-alpha-D-ribose 1-phosphate
-
competitive inhibition with regard to inosine
-
3-((2-pyrrolidine-1-yl)ethyl)uracil
Q8I3X4
inhibits both enzymic activity and growth of Plasmodium falciparum
3-(2-amino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-phenylpropanoic acid
-
IC50: 0.000047 mM
3-(3-chlorophenyl)-3-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)propanenitrile
-
IC50: 0.000011 mM
3-(3-chlorophenyl)-3-(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)propanenitrile
-
IC50: 0.00001 mM
3-(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-pyridin-3-ylpropanenitrile
-
IC50: 0.000039 mM
3-[(2R,5S)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-3,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one
-
6.4fold selectivity for Schistosoma enzyme over human enzyme
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
weak binding inhibitor
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
dissociation constant of 30 pM, an inhibitor release half-time of 64 min. Tight binding with 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A is in part due to a 2.7 A ionic interaction between a PO4 oxygen and the N1' cation of the hydroxypyrrolidine. The interaction of the inhibitor with TvPNP is the tightest-binding enzymatic interaction known for an immucillin analogue of adenosine; tight binding inhibitor
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-G
-
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
-
4-imino-7-methyl-1,2,3,4-tetrahydropyrazolo [1,5-a] [1,3,5] triazin-2-one
-
-
5'-chloro-5'-deoxy-8-aminoguanosine
-
-
5'-deoxy-5'-iodo-9-deazainosine
-
-
5'-deoxy-5'-iodoinosine
-
-
5'-deoxy-immucillin-H
-
-
5'-methylthio-immucillin-H
-
favors inhibition of Plasmodium falciparum purine nucleoside phosphorylase over human enzyme
-
5'-methylthio-immucillin-H
-
favours inhibition of Plasmodium falciparum purine nucleoside phosphorylase over human enzyme
-
5'-methylthio-immucillin-H
-
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3,3-dimethylpentane-1-sulfonamide
-
IC50: 0.0001 mM
5-amino-3-(2-thienylmethyl)-3,6-dihydro-7H-[1,2,3]triazolo[4,5-d]pyrimidin-7-one
-
IC50: 0.001839 mM
6-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-4,4-dimethylhexanamide
-
IC50: 0.2 mM
6-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-4,4-dimethylhexanenitrile
-
IC50: 0.00026 mM
6-amino-2-chloro-7-deazapurine 2'-deoxyriboside
-
-
6-amino-5-bromopyrimidine-2,4(1H,3H)-dione
-
formation of eight hydrogen bonds with key residues in the active site E203, N245 and T244
6-amino-7-(2-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00016 mM
6-amino-7-phenylethinyl-7-deazapurine 2'-deoxyriboside
-
-
6-benzyloxy-2-chloropurine
-
-
6-chloro-7-deazapurine 2'-deoxyriboside
-
-
6-hydroxy-9-p-aminobenzylpurine
-
-
6-Mercaptopurine
-
-
6-methylformycin
-
-
-
6-methylthiopurine
-
-
6-Thioxanthine
-
-
6-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
7-(3-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000028 mM
7-(cyclopentylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000029 mM
7-(pyridin-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00004 mM
7-benzyl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000035 mM
7-Deazaguanine
-
-
7-deazathioinosine
-
-
7-ketopyrazolo[4,3-d]pyrimidine
-
inhibits phosphorolysis of 7-methylguanosine uncompetitively, inhibits synthesis of 8-azaguanosine competitively
7-methylguanine
P81989
-
7-[(2S,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-2-yl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
7-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
i.e. forodesine
7-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidine-6-carboxylic acid
-
-
7-[3-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00019 mM
7-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
i.e. SerMe-immucillin-H. Kd-value 5.2 pM
7-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
i.e. SerMe-immucillin-H. Inhibitor is orally available with a long residence time on blood enzyme
7-[[(3R,4R)-4-hydroxy-3-(hydroxymethyl)pyrrolidin-2-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
i.e. BCX-4208, mimics the charged ribosyl oxocarbenium ion formed during the transition state
7KPP
-
non-substrate inhibitor of both the phosphorolytic and reverse synthetic pathways, inhibits synthesis of 8-azaguanosine from 8-azaguanine competitively
8-amino-3-(2-thienylmethyl)guanine
-
-
8-amino-5'-deoxy-5'-chloroguanosine
-
-
8-amino-5'-deoxy-5'-iodoguanosine
-
-
8-aminoguanine
-
-
8-aminoguanosine
-
-
8-aminoguanosine
-
-
8-aminoguanosine
-
-
8-aminoquinazolinone
-
-
8-aza-2,6-diamino-(S)-9-[2-(phosphonomethoxy)ethyl]-purine
P81989
-
8-aza-2,6-diaminopurine
-
noncompetitive
8-bromo-N(9)-acycloguanosine
-
-
9-(1,3-dihydroxy-2-propoxymethyl)guanine
-
-
9-(2'-benzyl-5',5'-difluoro-5'-phosphonopentyl)guanine
-
multisubstrate analogue inhibitor
9-(2-fluoro-3,4-dihydroxybutyl)-guanine
-
-
9-(2-hydroxyethoxymethyl)guanine
-
-
9-(2-phosphonylmethoxyethyl)-8-azaguanine
-
inhibits phosphorolytic and synthetic reaction, competitive
-
9-(2-phosphonylmethoxyethyl)-8-azaguanine
-
competitive versus 8-azaguanine and alpha-D-ribose 1-phosphate
-
9-(3,4-dihydroxybutyl)guanine
-
and analogues
9-(5',5'-difluoro-5'-phosphonobutyl)-9-deazaguanine
-
multisubstrate analogue inhibitor
9-(5',5'-difluoro-5'-phosphonoheptyl)-9-deazaguanine
-
multisubstrate analogue inhibitor
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
multisubstrate analogue inhibitor. Upon titration of the recombinant enzyme, endothermic as well as exothermic signals are obtained. Part of the recombinant enzyme forms a complex with its product, hypoxanthine, although hypoxanthine was not present at any purification stage except for its natural occurrence in Escherichia coli cells. Binding of hypoxanthine is accompanied with a large negative change of the free enthalpy, and therefore the replacement of hypoxanthine by 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine yields a positive heat signal
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
-
9-(5,5-difluoro-5-phosphonopentyl)guanine
-
-
9-(5,5-difluoro-5-phosphonopentyl)guanine
-
i.e. Danzin compound
9-(5,5-difluoro-5-phosphopentyl)guanine
-
-
9-(5,5-difluorophosphonopentyl)guanine
-
-
9-(5-phosphonopentyl)guanine
-
-
9-benzylguanine
-
-
9-benzylguanine
-
isothermal titration calorimetry study. At 25C, the tighter 9-benzylguanine binding reaction goes from an enthalpically-driven reaction in the absence of phosphate to an entropically-driven reaction at 10 mM phosphate, and the enthalpically-driven nature of the binding reaction is restored at 75 mM phosphate. Bound phosphate affects the interactions of the side-chains with the ribose catalytic site. 9-Benzylguanine interacts with Phe159 from an adjacent subunit
9-deazaguanine
-
multisubstrate analogue inhibitor
acyclovir
-
isothermal titration calorimetry study. At 25C and with an increase in the phosphate concentration from 0 to 50 mM, acyclovir binding becomes more entropically-driven
adenosine
-
binds to enzyme and behaves as a weak inhibitor of inosine phosphorolysis
adenosine
-
1 mM, about 20% inhibition
allopurinol
-
-
alpha-D-ribose 1-phosphate
-
product inhibition
alpha-D-ribose 1-phosphate
-
-
alpha-D-ribose 1-phosphate
-
product inhibition
alpha-D-ribose 1-phosphate
-
-
alpha-D-ribose 1-phosphate
-
-
alpha-D-ribose 1-phosphate
P81989
mixed inhibition of phosphorolysis of inosine
alpha-D-ribose 1-phosphate
-
mixed inhibition
aminopterin
-
noncompetitive
cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
with (hypoxanthine-9-yl)methyl aglycone
-
cis-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethylpyrrolidin-3-ol
-
-
D,L-6-methyl 5,6,7,8-tetrahydropterin
-
competitive
DADMe-immucillin-G
-
-
DADMe-immucillin-H
-
-
DATMe-immucillin-H
-
-
deoxyadenosine
-
-
deoxyimmucillin-H
-
-
deoxyimmucillin-H
Q2HXR2
-
Deoxyinosine
-
-
diphosphate
-
-
erythro-9-(2-hydroxy-3-nonyl)adenine
-
competitive
folate
-
0.05-0.1 mM, slight competitive inhibitor
folate
-
competitive
Formycin A
-
-
formycin B
-
-
formycin B
-
inhibits phosphorolysis of 7-methylguanosine uncompetitively; weak, uncompetitive inhibitor. Formycin B forms a weakly fluorescent complex with the enzyme
forodesine
-
a highly potent, orally active, rationally designed PNP inhibitor, that is active in preclinical studies with malignant cells and clinical utility against T-cell acute lymphoblastic leukemia and cutaneous T-cell lymphoma; a highly potent, orally active, rationally designed PNP inhibitor, use for the management of some B-cell malignancies
ganciclovir
-
-
ganciclovir
-
isothermal titration calorimetry study. At 25C and with an increase in the phosphate concentration from 0 to 50 mM, ganciclovir binding becomes more enthalpically-driven
Guanine
-
product inhibition
Guanine
-
product inhibition
Guanine
-
inhibits phosphorolysis of xanthosine
Guanine
P81989
competitive inhibition of hydrolysis of inosine
Guanine
-
competitive versus phosphate and inosine
guanosine
-
product inhibition
guanosine
-
strong competitive inhibitor with deoxyinosine as substrate
guanosine
-
-
guanosine
-
inhibits phosphorolysis of xynthosine
guanosine
P81989
uncompetitive with alpha-D-1-ribose 1-phosphate as varied substrate
guanosine
-
uncompetitive inhibition of alpha-D-ribose 1-phosphate
hypoxanthine
-
-
hypoxanthine
-
product inhibition
hypoxanthine
-
inhibits phosphorolysis of xanthosine
hypoxanthine arabinoside
-
-
immucillin-A
-
weak binding inhibitor
immucillin-A
-
slow-onset tight binding inhibition, inhibitor release half-time of 17.2 min; slow-onset tightbinding inhibition with TvPNP, to give an equilibrium dissociation constant of 87 pM and an inhibitor release half-time of 17.2 min
immucillin-G
-
dissociation constant of 42 pM
immucillin-G
-
dissociation constant of 900 pM
immucillin-G
Q2HXR2
-
immucillin-G
-
-
immucillin-H
-
dissociation constant: 23 pM
immucillin-H
-
dissociation constant: 56 pM
Inosine
-
strong competitive inhibitor with deoxyinosine as substrate
Inosine
-
inhibits ribosylation of hypoxanthine and guanine
L-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
-
N(1)-Methylformycin A
-
-
N(6)-methyl-formycin A
-
-
N(6)-Methylformycin A
-
competitive with respect to inosine, 7-methylguanosine and 7-methyladenosine
N(6)-Methylformycin A
-
-
N(6)-methylformycin B
-
-
N(7)-acycloguanosine
-
-
N(7)-acycloguanosine
-
-
N(7)-methylformycin A
-
-
N(9)-acycloguanosine
-
-
N(9)-acycloguanosine
-
-
Oxoallopurinol
-
-
oxoformycin A
-
weak
-
oxoformycin B
-
weak
PCMB
-
adenosine-specific phosphorylase
PCMB
-
no inhibition of PUNPII
PCMB
-
0.1 mM, 85% loss of activity within 15 min
PCMB
-
dithiothreitol restores activity
phosphate
-
-
phosphate
-
product inhibition
phosphate
P81989
-
phosphate
-
uncompetitive versus guanine
purine riboside
-
-
quinazolinone
-
-
SerMe-immucillin-H
-
-
xanthine
-
poor inhibitor of phosphorolysis of guanosine
Xanthosine
-
poor inhibitor of phosphorolysis of guanosine
L-immucillin-H
-
-
additional information
-
the enzyme inhibitors 8-amino-5'-deoxy-5'-chloroguanosine and 8-amino-9-benzylguanine may have some antimalarial potential by limiting hypoxanthine production in the parasite-infected erythrocyte
-
additional information
-
inhibition by 9-cycloaliphatic methyl and 9-saturated heterocyclic methyl-9-deazapurines
-
additional information
Q2HXR2
insensitive to methylthio-immucillin-H
-
additional information
-
thermodynamics for binding the immucillins to the first subunit of human purine nucleoside phosphorylase. Titrations of purine nucleoside phosphorylase using isothermal calorimetry indicate that binding of a structurally rigid first-generation immucillin-H is driven by large negative enthalpy values with a substantial entropic penalty. The tightest-binding inhibitors have increased conformational flexibility. Despite their conformational freedom in solution, flexible inhibitors bind with high affinity because of reduced entropic penalties. Entropic penalties are proposed to arise from conformational freezing of the purine nucleoside phosphorylase-inhibitor complex with the entropy term dominated by protein dynamics. The conformationally flexible immucillins reduce the system entropic penalty. Disrupting the ribosyl 5'-hydroxyl interaction of transition state analogues with purine nucleoside phosphorylase causes favorable entropy of binding. Tight binding of the immucillins is characterized by large enthalpic contributions, emphasizing their similarity to the transition state
-
additional information
-
multisubstrate analogue inhibitors with 9-deazaguanine aglycone have better anti-leukaemic and anti-lymphoma activities compared to the guanine and hypoxanthine analogues, and applied in the concentration of 100 microM, cause a statistically significant decrease in the cell viability in all human leukemia and lymphoma cells used. No differences are observed between the effects on the growth of tumour cells sensible to the inhibition of purine nucleoside phosphorylase, such as human adult T-cell leukemia and lymphoma cells, and other investigated cells
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Inosine
-
no substrate activation
Inosine
-
substrate activation
Inosine
-
activates the crystalline enzyme
staurosporine
-
stimulation
thapsigargin
-
stimulation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.022
-
1-Methylguanosine
-
-
0.05
-
1-methylinosine
-
-
0.391
-
2',3'-dideoxyinosine
-
mutant Y88L, pH 7.0, 25C
0.45
-
2',3'-dideoxyinosine
-
mutant Y88F, pH 7.0, 25C
0.645
-
2',3'-dideoxyinosine
-
mutant Y88M, pH 7.0, 25C
0.675
-
2',3'-dideoxyinosine
-
mutant Y88H, pH 7.0, 25C
0.81
-
2',3'-dideoxyinosine
-
mutant Y88C, pH 7.0, 25C
0.86
-
2',3'-dideoxyinosine
-
mutant Y88Q, pH 7.0, 25C
0.98
-
2',3'-dideoxyinosine
-
mutant Y88W, pH 7.0, 25C
1.03
-
2',3'-dideoxyinosine
-
wild-type, pH 7.0, 25C
1.67
-
2',3'-dideoxyinosine
-
mutant Y88A, pH 7.0, 25C
2.6
-
2',3'-dideoxyinosine
-
-
4.06
-
2',3'-dideoxyinosine
-
mutant Y88S, pH 7.0, 25C
4.41
-
2',3'-dideoxyinosine
-
mutant Y88V, pH 7.0, 25C
4.67
-
2',3'-dideoxyinosine
-
mutant Y88I, pH 7.0, 25C
8.16
-
2',3'-dideoxyinosine
-
mutant Y88T, pH 7.0, 25C
9.53
-
2',3'-dideoxyinosine
-
mutant Y88M, pH 7.0, 25C
12.7
-
2',3'-dideoxyinosine
-
mutant Y88D, pH 7.0, 25C
14.9
-
2',3'-dideoxyinosine
-
mutant Y88E, pH 7.0, 25C
0.19
-
2'-deoxyadenosine
-
-
0.015
-
2'-deoxyguanosine
-
pH 7.0, 25C
0.024
-
2'-deoxyguanosine
-
-
0.05
-
2'-deoxyguanosine
A4Q998, -
-
0.1
-
2'-deoxyguanosine
-
-
1.01
-
2'-deoxyguanosine
Q72IR2
pH 7.0, 80C
1.9
-
2'-deoxyguanosine
-
-
0.043
-
2'-deoxyinosine
-
-
0.062
-
2'-deoxyinosine
-
-
0.068
-
2'-deoxyinosine
-
pH 7.0, 25C
0.079
-
2'-deoxyinosine
-
pH 7.4
0.091
-
2'-deoxyinosine
-
pH 7.4
0.19
-
2'-deoxyinosine
A4Q998, -
-
0.92
-
2'-deoxyinosine
-
-
2.06
-
2'-deoxyinosine
Q72IR2
pH 7.0, 80C
0.013
-
2-amino-6-mercapto-7-methylpurine
-
-
0.029
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
-
0.1425
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
pH 7.6
0.023
-
2-fluoro-2'-deoxyadenosine
-
pH 7.4, 25C
0.264
-
2-fluoro-2'-deoxyadenosine
-
mutant E201Q/N243D
0.012
-
2-Fluoroadenosine
-
pH 7.4, 25C
0.15
-
3-(beta-D-ribofuranosyl)adenine
-
-
0.8
-
3-(beta-D-ribofuranosyl)adenine
-
-
0.22
-
3-(beta-D-ribofuranosyl)hypoxanthine
-
-
0.26
-
3-(beta-D-ribofuranosyl)hypoxanthine
-
-
0.6
-
5'-deoxyinosine
-
-
0.0106
-
5'-methylthioadenosine
-
pH 7.4
0.0319
-
5'-methylthioadenosine
Q2HXR2
pH 7.4
0.167
-
6-mercaptoguanosine
-
-
0.07
-
6-mercaptopurine riboside
-
-
0.065
-
7,8-dimethylguanosine
-
-
0.027
-
7-(beta-D-ribofuranosyl)guanine
-
-
0.108
-
7-(beta-D-ribofuranosyl)guanine
-
-
0.64
-
7-(beta-D-ribofuranosyl)hypoxanthine
-
-
1.26
-
7-(beta-D-ribofuranosyl)hypoxanthine
-
-
1.52
-
7-(beta-D-ribofuranosyl)hypoxanthine
-
-
0.034
-
7-butylguanosine
-
-
0.013
-
7-ethylguanosine
-
-
0.069
-
7-isopropylguanine
-
-
0.358
-
7-methyl-6-thio-guanosine
-
pH 7.6
0.0147
-
7-methylguanosine
-
-
0.015
-
7-methylguanosine
-
-
0.016
-
7-methylguanosine
-
-
0.0168
-
7-methylguanosine
-
pH 7.0, 25C, recombinant enzyme
0.0168
-
7-methylguanosine
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0264
-
7-methylguanosine
P81989
25C, pH 7.0
0.05
-
7-methylguanosine
-
mutant F159W, pH not specified in the publication, temperature not specified in the publication
0.07
-
7-methylguanosine
-
mutant F200W, pH not specified in the publication, temperature not specified in the publication
0.6
-
7-methylguanosine
-
-
9.2
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
12.5
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
13
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
13.9
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
16.3
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
39.3
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
0.033
-
7-Methylinosine
-
pH 7.0, 25C
0.169
-
7-Methylinosine
-
-
0.247
-
7-Methylinosine
-
-
0.27
-
7-Methylinosine
-
-
0.37
-
7-Methylinosine
-
-
0.6
-
7-Methylinosine
-
-
0.012
-
7-N-methyl-6-thiopurine riboside
-
enzyme purified from mantle cell lymphoma, pH 7.5, temperature not specified in the publication
-
0.04
-
7-N-methyl-6-thiopurine riboside
-
enzyme purified from diffuse large B-cell lymphoma progressed from follicular lymphoma, pH 7.5, temperature not specified in the publication
-
0.06
-
7-N-methyl-6-thiopurine riboside
-
recombinant enzyme, pH 7.5, temperature not specified in the publication
-
0.08
-
7-N-methyl-6-thiopurine riboside
-
enzyme purified from peripheral blood mononuclear cell, pH 7.5, temperature not specified in the publication
-
0.025
-
7-prolylguanosine
-
-
0.09
-
8-Azaguanine
-
pH 5.9
0.1
-
8-Azaguanine
-
-
0.7
-
8-Azaguanine
-
pH 8
0.661
-
8-Azahypoxanthine
-
-
0.1
-
8-methylguanosine
-
-
0.126
-
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine
-
pH 7.4, 25C
0.009
-
9-beta-D-ribofuranosyl-6-methylthiopurine
-
pH 7.4, 25C
0.34
-
adenine
-
-
0.41
-
adenine
-
-
0.0061
-
adenosine
-
; 25C, pH 7.4
0.012
-
adenosine
-
-
0.028
-
adenosine
-
mutant enzyme N243D/K244Q, pH 6.0
0.042
-
adenosine
-
mutant enzyme N243D/K244Q, pH 7.0
0.045
-
adenosine
-
mutant enzyme N243D, pH 6.0 ot pH 7.0
0.046
-
adenosine
-
pH 7.4, 25C
0.13
-
adenosine
-
-
0.262
-
adenosine
P45563
pH 7.1, 25C, mutant enzyme N239D
6.1
-
adenosine
-
-
0.1
-
alpha-D-deoxyribose 1-phosphate
-
reaction with adenine
0.129
-
alpha-D-deoxyribose 1-phosphate
-
reaction with hypoxanthine
0.133
-
alpha-D-deoxyribose 1-phosphate
-
reaction with guanine
0.04
-
alpha-D-ribose 1-phosphate
-
reaction with adenine
0.041
-
alpha-D-ribose 1-phosphate
-
reaction with guanine
0.042
-
alpha-D-ribose 1-phosphate
-
reaction with hypoxanthine
0.059
-
alpha-D-ribose 1-phosphate
-
-
0.134
-
alpha-D-ribose 1-phosphate
-
-
0.2
-
alpha-D-ribose 1-phosphate
-
-
0.228
-
alpha-D-ribose 1-phosphate
-
reaction with guanine
0.8
-
arsenate
-
reaction with inosine
1.8
-
arsenate
-
-
0.145
-
deoxyadenosine
-
-
0.02
-
deoxyguanosine
-
-
0.027
-
deoxyguanosine
-
-
0.038
-
deoxyguanosine
-
-
0.044
-
deoxyguanosine
-
-
0.0443
-
deoxyguanosine
-
-
0.057
-
deoxyguanosine
-
-
0.1
-
deoxyguanosine
-
-
0.18
-
deoxyguanosine
-
-
0.33
-
deoxyguanosine
-
-
0.35
-
deoxyguanosine
-
-
0.027
-
Deoxyinosine
-
-
0.066
-
Deoxyinosine
-
-
0.091
-
Deoxyinosine
-
pH 7.4
0.18
-
Deoxyinosine
-
-
0.259
-
Deoxyinosine
Q2HXR2
pH 7.4
0.67
-
Deoxyinosine
-
-
0.1
-
deoxyribose 1-phosphate
-
reaction with adenine
0.006
-
Guanine
-
pH 7.0
0.006
-
Guanine
-
recombinant erythrocyte enzyme
0.009
-
Guanine
-
pH 7.5
0.011
-
Guanine
-
pH 6.0
0.0138
-
Guanine
-
pH 7.0
0.014
-
Guanine
-
reaction with ribose 1-phosphate
0.017
-
Guanine
-
reaction with deoxyribose 1-phosphate
0.0182
-
Guanine
-
-
0.022
-
Guanine
-
pH 7.5
0.029
-
Guanine
-
pH 6.0
0.03
-
Guanine
-
-
0.0308
-
Guanine
-
-
0.8
-
Guanine
-
-
0.0041
-
guanosine
-
-
0.0089
-
guanosine
-
pH 7.4
0.0094
-
guanosine
-
pH 7.4
0.0094
-
guanosine
Q2HXR2
pH 7.4
0.0094
-
guanosine
-
pH 7.4
0.01
-
guanosine
-
-
0.011
-
guanosine
-
pH 7.0
0.012
-
guanosine
-
pH 7.0
0.012
-
guanosine
-
recombinant erythrocyte enzyme
0.012
-
guanosine
-
pH 7.0, 25C
0.014
-
guanosine
-
pH 6.5
0.0159
-
guanosine
-
pH 6.5
0.018
-
guanosine
-
-
0.02
-
guanosine
-
-
0.022
-
guanosine
-
-
0.029
-
guanosine
-
-
0.029
-
guanosine
-
-
0.035
-
guanosine
-
-
0.042
-
guanosine
-
wild-tpe, pH 7.4, 25C
0.0435
-
guanosine
-
-
0.0443
-
guanosine
-
-
0.046
-
guanosine
-
pH 5.7
0.047
-
guanosine
-
-
0.047
-
guanosine
-
mutant L22E/H104R, pH 7.4, 25C
0.049
-
guanosine
-
pH 7.4, 25C
0.05
-
guanosine
-
-
0.059
-
guanosine
-
mutant E201Q/N243D
0.06
-
guanosine
-
-
0.06
-
guanosine
-
; 25C, pH 7.4
0.091
-
guanosine
-
pH 5.7
0.11
-
guanosine
-
-
0.11
-
guanosine
-
-
0.12
-
guanosine
-
-
0.122
-
guanosine
-, Q8U2I1
80C; 80C
0.155
-
guanosine
P45563
pH 7.1, 25C, wild-type enzyme
0.187
-
guanosine
A4Q998, -
-
0.19
-
guanosine
-
-
0.206
-
guanosine
-, Q9KCN8
pH 7.5
0.216
-
guanosine
-
-
0.249
-
guanosine
P45563
pH 7.1, 25C, mutant enzyme Y191L
0.53
-
guanosine
Q72IR2
pH 7.0, 80C
1.85
-
guanosine
-
at 40C
59.7
-
guanosine
-
-
0.0033
-
hypoxanthine
-
-
0.01
-
hypoxanthine
-
mutant enzyme Y88F
0.013
-
hypoxanthine
-
mutant enzyme S33A
0.019
-
hypoxanthine
-
-
0.019
-
hypoxanthine
-
mutant enzyme H86A
0.02
-
hypoxanthine
-
-
0.02
-
hypoxanthine
-
with ribose 1-phosphate
0.02
-
hypoxanthine
-
-
0.02
-
hypoxanthine
-
mutant enzyme R84A and H257A
0.021
-
hypoxanthine
-
reaction with deoxyribose 1-phosphate
0.024
-
hypoxanthine
-
mutant enzyme T242A
0.025
-
hypoxanthine
-
mutant enzyme F159A
0.03
-
hypoxanthine
-
wild-type enzyme
0.03
-
hypoxanthine
-
recombinant erythrocyte enzyme
0.039
-
hypoxanthine
-
-
0.04
-
hypoxanthine
-
mutant enzyme K244A
0.082
-
hypoxanthine
-
-
0.18
-
hypoxanthine
-
mutant enzyme M219A
0.27
-
hypoxanthine
-
-
0.4
-
hypoxanthine
-
-
0.57
-
hypoxanthine
-
mutant enzyme F200A
1
-
hypoxanthine
-
mutant enzyme E201A
2.3
-
hypoxanthine
-
mutant enzyme N243A
45.6
-
hypoxanthine
-
-
1e-05
-
Inosine
-
mutant T90R/T156A, pH 7.6, 37C
2e-05
-
Inosine
-
mutant T90R, pH 7.6, 37C
3e-05
-
Inosine
-
mutant T156S, pH 7.6, 37C
4e-05
-
Inosine
-
mutant V206I, pH 7.6, 37C
0.00031
-
Inosine
-
isoform of 816 amino acids, pH 7.0, 37C
0.00039
-
Inosine
-
wild-type, pH 7.6, 37C
0.00045
-
Inosine
-
pH 7.0, 37C
0.00085
-
Inosine
-
pH 7.0, 37C
0.00092
-
Inosine
-
mutant T90S, pH 7.6, 37C
0.00135
-
Inosine
-
mutant T156A, pH 7.6, 37C
0.0047
-
Inosine
-
pH 7.5, 25C
0.0047
-
Inosine
-
pH 7.4
0.005
-
Inosine
Q8I3X4
pH 7.5, 25C
0.0078
-
Inosine
-
-
0.0086
-
Inosine
-
pH 7.4
0.012
-
Inosine
-
isoform of 702 amino acids, pH 7.0, 37C
0.013
-
Inosine
-
-
0.0131
-
Inosine
Q2HXR2
pH 7.4
0.0134
-
Inosine
-
pH 7.0, 25C, recombinant enzyme
0.0134
-
Inosine
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.016
-
Inosine
-
pH 7.4, 25C
0.022
-
Inosine
-
mutant enzymes N243A and T242A
0.025
-
Inosine
-
; 25C, pH 7.4
0.027
-
Inosine
-
mutant L22E/H104R, pH 7.4, 25C
0.028
-
Inosine
-
-
0.03
-
Inosine
-
wild-tpe, pH 7.4, 25C
0.032
-
Inosine
-
-
0.032
-
Inosine
-
-
0.032
-
Inosine
-
wild-type enzyme, pH 6.0
0.032
-
Inosine
-
wild-type, pH 7.5, 25C
0.036
-
Inosine
-
-
0.037
-
Inosine
-
mutant enzyme K244A
0.038
-
Inosine
-
pH 7.5, 25C
0.038
-
Inosine
-
mutant V39T/N123L/R210Q, pH 7.5, 25C
0.039
-
Inosine
-
-
0.039
-
Inosine
-
mutant N123L/R210Q, pH 7.5, 25C
0.04
-
Inosine
-
wild-type enzyme
0.04
-
Inosine
-
recombinant erythrocyte enzyme
0.04
-
Inosine
-
pH 7.0, 25C
0.04
-
Inosine
-
pH 7.5, 25C
0.041
-
Inosine
-
-
0.043
-
Inosine
-
-
0.047
-
Inosine
-
-
0.048
-
Inosine
-
-
0.048
-
Inosine
-
wild-type, pH 7.0, 25C
0.05
-
Inosine
-
-
0.05
-
Inosine
-
-
0.0556
-
Inosine
-
-
0.058
-
Inosine
-
mutant enzyme Y88F
0.058
-
Inosine
-
wild-type enzyme, pH 7.0
0.07
-
Inosine
-
-
0.073
-
Inosine
-
mutant Y88F, pH 7.0, 25C
0.074
-
Inosine
-
mutant Y88H, pH 7.0, 25C
0.08
-
Inosine
-
mutant Y88A, pH 7.0, 25C
0.0823
-
Inosine
-
mutant enzyme N243D/K244Q, pH 7.0
0.09
-
Inosine
-
-
0.094
-
Inosine
-
mutant Y88L, pH 7.0, 25C
0.096
-
Inosine
-
pH 7.4, 25C
0.1
-
Inosine
-
-
0.104
-
Inosine
-
mutant T90A/T156A, pH 7.6, 37C
0.11
-
Inosine
-
enzyme from vegetative cells
0.13
-
Inosine
-
mutant enzyme R84A
0.14
-
Inosine
-
enzyme from spores
0.14
-
Inosine
-
mutant enzyme S33A
0.17
-
Inosine
-
-
0.17
-
Inosine
-
mutant Y88C, pH 7.0, 25C
0.2
-
Inosine
-
inosine-guanosine phosphorylase
0.21
-
Inosine
-
mutant enzyme H257A
0.22
-
Inosine
-
mutant enzyme E89A
0.231
-
Inosine
P45563
pH 7.1, 25C, mutant enzyme Y191L
0.236
-
Inosine
-, Q9KCN8
pH 7.5
0.245
-
Inosine
-
mutant F159W, pH not specified in the publication, temperature not specified in the publication
0.28
-
Inosine
-
mutant enzyme H86A
0.31
-
Inosine
-
-
0.31
-
Inosine
-
mutant enzyme F159A
0.32
-
Inosine
-
25C, pH 7.4
0.32
-
Inosine
-
37C
0.322
-
Inosine
-, Q8U2I1
80C; 80C, pH 7.4
0.326
-
Inosine
-
mutant F200W, pH not specified in the publication, temperature not specified in the publication
0.34
-
Inosine
-
-
0.345
-
Inosine
-
mutant Y88M, pH 7.0, 25C
0.37
-
Inosine
-
mutant Y88R, pH 7.0, 25C
0.39
-
Inosine
-
pH 7.6, 37C
0.43
-
Inosine
-
mutant Y88S, pH 7.0, 25C
0.484
-
Inosine
-
mutant enzyme N243D, pH 7.0
0.491
-
Inosine
-
pH 7.6, 37C
0.5
-
Inosine
-
mutant Y88W, pH 7.0, 25C
0.585
-
Inosine
A4Q998, -
-
0.67
-
Inosine
A3F8V1, -
-
0.691
-
Inosine
-
mutant enzyme N243D, pH 6.0
0.729
-
Inosine
-
mutant enzyme N243D/K244Q, pH 6.0
0.75
-
Inosine
-
25C, pH 7.4
0.8
-
Inosine
-
40C
0.83
-
Inosine
-
50C
0.845
-
Inosine
-
mutant Y88M, pH 7.0, 25C
0.855
-
Inosine
-
mutant Y88Q, pH 7.0, 25C
0.963
-
Inosine
P45563
pH 7.1, 25C, wild-type enzyme
1.2
-
Inosine
-
mutant Y88T, pH 7.0, 25C; mutant Y88V, pH 7.0, 25C
1.35
-
Inosine
-
25C, pH 7.4
1.42
-
Inosine
-
mutant Y88I, pH 7.0, 25C
1.796
-
Inosine
-
pH 7.6, 37C
1.92
-
Inosine
-
at 40C
2.08
-
Inosine
Q72IR2
pH 7.0, 80C
2.2
-
Inosine
-
60C
2.6
-
Inosine
-
mutant Y88D, pH 7.0, 25C
4.86
-
Inosine
-
mutant Y88E, pH 7.0, 25C
8
-
Inosine
-
mutant enzyme M219A
8.4
-
Inosine
-
mutant enzyme E201A
19
-
Inosine
-
mutant enzyme F200A
143.1
-
Inosine
-
mutant T90A, pH 7.6, 37C
0.09
-
isobutylguanosine
-
-
0.005
-
Methylthioinosine
-
pH 7.4
0.0108
-
Methylthioinosine
-
pH 7.4
0.012
-
Methylthioinosine
-
pH 7.5, 25C
0.62
-
Nicotinamide riboside
-
-
1.48
-
Nicotinamide riboside
-
-
0.09
-
p-nitrophenyl beta-D-ribofuranoside
-
; 25C, pH 7.4
0.013
-
phosphate
-
reaction with inosine
0.015
-
phosphate
-
reaction with guanosine
0.028
-
phosphate
-
reaction with inosine
0.032
-
phosphate
-
-
0.0343
-
phosphate
P81989
25C, pH 7.0, cosubstrate: 7-methylguanosine
0.05
-
phosphate
-
enzyme purified from peripheral blood mononuclear cell, pH 7.5, temperature not specified in the publication
0.053
-
phosphate
-
-
0.0578
-
phosphate
-
-
0.094
-
phosphate
-
pH 7.6
0.1
-
phosphate
-
recombinant enzyme, pH 7.5, temperature not specified in the publication
0.12
-
phosphate
-
reaction with inosine
0.132
-
phosphate
P81989
25C, pH 7.0, cosubstrate: inosine
0.135
-
phosphate
-
reaction with 7-methylinosine
0.167
-
phosphate
-
reaction with 7-methylinosine
0.1868
-
phosphate
-
pH 7.6
0.21
-
phosphate
A3F8V1, -
-
0.22
-
phosphate
-
reaction with inosine, mutant enzyme T242A
0.34
-
phosphate
-
37C
0.376
-
phosphate
-
-
0.427
-
phosphate
-
cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25C; cosubstrate 7-methylinosine, pH 7.0, 25C
0.43
-
phosphate
-
-
0.52
-
phosphate
-
-
0.583
-
phosphate
-
cosubstrate inosine, pH 7.0, 25C
0.68
-
phosphate
-
-
0.74
-
phosphate
-
-
0.76
-
phosphate
-
-
1.1
-
phosphate
-
-
1.131
-
phosphate
-
cosubstrate 2'-deoxyinosine, pH 7.0, 25C
1.345
-
phosphate
-
cosubstrate guanosine, pH 7.0, 25C
2.1
-
phosphate
-
mutant enzyme Y88F
2.349
-
phosphate
-
cosubstrate 2'-deoxyguanosine, pH 7.0, 25C
2.5
-
phosphate
-
mutant enzymes H257A and F159A
2.7
-
phosphate
-
reaction with inosine, mutant enzyme K244A
3.5
-
phosphate
-
reaction with inosine, mutant enzyme N243A
3.6
-
phosphate
-
reaction with inosine, mutant enzyme S33A
3.9
-
phosphate
-
reaction with inosine, inosine-guanosine phosphorylase
4
-
phosphate
-
reaction with inosine, wild-type enzyme
4
-
phosphate
-
recombinant erythrocyte enzyme
4.3
-
phosphate
-
mutant enzyme M219A
4.5
-
phosphate
-
reaction with inosine, mutant enzyme F200A; reaction with inosine, mutant enzyme R84A
5.1
-
phosphate
-
enzyme from vegetative cells
6.1
-
phosphate
-
reaction with inosine, mutant enzyme E201A
7.2
-
phosphate
-
enzyme from spores
9.2
-
phosphate
-
reaction with inosine, mutant enzyme H86A
13.1
-
phosphate
-
reaction with guanosine
21.5
-
phosphate
-
-
91
-
phosphate
-
reaction with inosine, mutant enzyme E89A
0.5
-
ribose 1-phosphate
-
-
0.085
-
uridine
Q8I3X4
pH 7.5, 25C
0.115
-
uridine
-
pH 7.4
0.28
-
xanthine
-
pH 6.0
0.306
-
xanthine
-
pH 7.5
0.38
-
xanthine
-
pH 6.0
0.425
-
xanthine
-
pH 7.0
0.051
-
Xanthosine
-
-
0.072
-
Xanthosine
P45563
pH 7.1, 25C, wild-type enzyme
0.095
-
Xanthosine
-
-
0.4
-
Xanthosine
-
pH 5.7
0.45
-
Xanthosine
-
-
0.58
-
Xanthosine
-
pH 5.7
1.1
-
Xanthosine
-
pH 6.5
1.6
-
Xanthosine
-
pH 6.5
0.016
-
Methylthioinosine
-
pH 7.5, 25C
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
2',3'-dideoxyinosine
-
mutant Y88E, pH 7.0, 25C
0.02
-
2',3'-dideoxyinosine
-
mutant Y88D, pH 7.0, 25C
0.09
-
2',3'-dideoxyinosine
-
mutant Y88W, pH 7.0, 25C
0.15
-
2',3'-dideoxyinosine
-
mutant Y88Q, pH 7.0, 25C
0.4
-
2',3'-dideoxyinosine
-
mutant Y88T, pH 7.0, 25C
0.5
-
2',3'-dideoxyinosine
-
mutant Y88I, pH 7.0, 25C
0.6
-
2',3'-dideoxyinosine
-
mutant Y88V, pH 7.0, 25C
0.9
-
2',3'-dideoxyinosine
-
wild-type, pH 7.0, 25C
1.5
-
2',3'-dideoxyinosine
-
mutant Y88M, pH 7.0, 25C
2.5
-
2',3'-dideoxyinosine
-
mutant Y88C, pH 7.0, 25C; mutant Y88H, pH 7.0, 25C
2.7
-
2',3'-dideoxyinosine
-
mutant Y88S, pH 7.0, 25C
2.8
-
2',3'-dideoxyinosine
-
mutant Y88A, pH 7.0, 25C
5.2
-
2',3'-dideoxyinosine
-
mutant Y88M, pH 7.0, 25C
6.1
-
2',3'-dideoxyinosine
-
mutant Y88L, pH 7.0, 25C
10.5
-
2',3'-dideoxyinosine
-
mutant Y88F, pH 7.0, 25C
5.1
-
2'-deoxyguanosine
-
pH 7.0, 25C
5.4
-
2'-deoxyguanosine
A4Q998, -
-
45
-
2'-deoxyguanosine
-
-
830
-
2'-deoxyguanosine
Q72IR2
pH 7.0, 80C
0.9
-
2'-deoxyinosine
-
pH 7.4
1.2
-
2'-deoxyinosine
-
pH 7.4
7.5
-
2'-deoxyinosine
-
pH 7.0, 25C
54
-
2'-deoxyinosine
A4Q998, -
-
85
-
2'-deoxyinosine
-
-
627
-
2'-deoxyinosine
Q72IR2
pH 7.0, 80C
17.3
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
-
0.155
-
2-fluoro-2'-deoxyadenosine
-
mutant E201Q/N243D
2.77
-
2-fluoro-2'-deoxyadenosine
-
pH 7.4, 25C
2.05
-
2-Fluoroadenosine
-
pH 7.4, 25C
0.027
-
5'-methylthioadenosine
Q2HXR2
pH 7.4
2.6
-
5'-methylthioadenosine
-
pH 7.4
6.23
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
9.2
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
20.4
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
23
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
24.7
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
73.1
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
2.57
-
7-Methylinosine
-
-
18
-
7-Methylinosine
-
pH 7.0, 25C
27.5
-
7-Methylinosine
-
-
9.7
-
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine
-
pH 7.4, 25C
1.1
-
9-beta-D-ribofuranosyl-6-methylthiopurine
-
pH 7.4, 25C
0.282
-
adenosine
-
mutant enzyme N243D, pH 7.0
0.579
-
adenosine
-
mutant enzyme N243D/K244Q, pH 7.0
0.71
-
adenosine
-
mutant enzyme N243D, pH 6.0
1.14
-
adenosine
-
mutant enzyme N243D/K244Q, pH 6.0
1.41
-
adenosine
-
mutant enzyme N243D/K244Q, pH 6.0
1.7
-
adenosine
-
; 25C, pH 7.4
3.3
-
adenosine
-
pH 7.4, 25C
115
-
alpha-D-deoxyribose 1-phosphate
-
reaction with hypoxanthine
145
-
alpha-D-deoxyribose 1-phosphate
-
reaction with guanine
100
-
alpha-D-ribose 1-phosphate
-
reaction with hypoxanthine
120
-
alpha-D-ribose 1-phosphate
-
reaction with guanine
0.48
-
Deoxyinosine
Q2HXR2
pH 7.4
0.89
-
Deoxyinosine
-
pH 7.4
48
-
Guanine
-
recombinant erythrocyte enzyme
127
-
Guanine
-
reaction with ribose 1-phosphate
158
-
Guanine
-
reaction with deoxyribose 1-phosphate
1
-
guanosine
A4Q998, -
-
1.1
-
guanosine
-
; 25C, pH 7.4
2
8
guanosine
-
recombinant erythrocyte enzyme
2.6
-
guanosine
-
pH 7.4
3.6
-
guanosine
Q2HXR2
pH 7.4
3.8
-
guanosine
-
pH 7.4
4
-
guanosine
-
pH 7.0, 25C
12
-
guanosine
-, Q9KCN8
pH 7.5
27
-
guanosine
-
pH 7.4, 25C
28.05
-
guanosine
-, Q8U2I1
80C
30
-
guanosine
-
mutant L22E/H104R, pH 7.4, 25C
31
-
guanosine
-
wild-tpe, pH 7.4, 25C
59
-
guanosine
-
-
60
-
guanosine
-
mutant E201Q/N243D
296
-
guanosine
Q72IR2
pH 7.0, 80C
0.5
-
hypoxanthine
-
mutant enzyme R84A
0.97
-
hypoxanthine
-
mutant enzyme H86A
1.4
-
hypoxanthine
-
mutant enzyme E201A
2.1
-
hypoxanthine
-
mutant enzyme S33A
9
-
hypoxanthine
-
mutant enzyme Y88F
10
-
hypoxanthine
-
mutant enzyme H257A
13
-
hypoxanthine
-
mutant enzyme N243A
14
-
hypoxanthine
-
mutant enzyme F200A
24
-
hypoxanthine
-
mutant enzyme F159A
58
-
hypoxanthine
-
mutant enzyme T242A
98
-
hypoxanthine
-
mutant enzyme K244A
103
-
hypoxanthine
-
reaction with ribose 1-phosphate
110
-
hypoxanthine
-
wild-type enzyme
110
-
hypoxanthine
-
recombinant erythrocyte enzyme
127
-
hypoxanthine
-
reaction with deoxyribose 1-phosphate
130
-
hypoxanthine
-
mutant enzyme M219A
0.01
-
Inosine
-
mutant Y88R, pH 7.0, 25C
0.06
-
Inosine
-
mutant enzyme N243A
0.386
-
Inosine
-
mutant enzyme N243D, pH 7.0
0.7
-
Inosine
-
mutant Y88E, pH 7.0, 25C
0.8
-
Inosine
-
mutant Y88D, pH 7.0, 25C
0.9
-
Inosine
-
mutant T90A/T156A, pH 7.6, 37C
1.1
-
Inosine
-
mutant enzyme S33A
1.1
-
Inosine
-
pH 7.4
1.2
-
Inosine
-
mutant T90A, pH 7.6, 37C
1.3
-
Inosine
-
25C, pH 7.4
1.3
-
Inosine
-
mutant Y88I, pH 7.0, 25C
1.4
-
Inosine
-
mutant Y88Q, pH 7.0, 25C
1.7
-
Inosine
-
pH 7.5, 25C
1.75
-
Inosine
-
25C, pH 7.4
1.76
-
Inosine
-
mutant enzyme N243D, pH 6.0
1.8
-
Inosine
-
; 25C, pH 7.4
2.1
-
Inosine
-
mutant enzyme R84A
2.3
-
Inosine
-
mutant Y88T, pH 7.0, 25C
2.4
-
Inosine
-
mutant Y88V, pH 7.0, 25C
2.6
-
Inosine
Q2HXR2
pH 7.4
3.5
-
Inosine
-
pH 7.4
4
-
Inosine
-
pH 7.4, 25C
4.2
-
Inosine
-
mutant enzyme E201A
5.4
-
Inosine
-
pH 7.0, 25C
5.5
-
Inosine
-
mutant Y88C, pH 7.0, 25C
5.6
-
Inosine
-
mutant Y88W, pH 7.0, 25C
6.55
-
Inosine
-
25C, pH 7.4
8.6
-
Inosine
-
mutant Y88H, pH 7.0, 25C
8.8
-
Inosine
-
mutant Y88S, pH 7.0, 25C
9.1
-
Inosine
-
mutant enzyme N243D/K244Q, pH 7.0
9.4
-
Inosine
-
mutant enzyme F200A
9.7
-
Inosine
-
mutant Y88M, pH 7.0, 25C
12.8
-
Inosine
-
mutant Y88M, pH 7.0, 25C
14
-
Inosine
-
mutant enzymes T242A and H86A
15.9
-
Inosine
-
mutant Y88L, pH 7.0, 25C
16
-
Inosine
-
mutant enzyme H257A
17.6
-
Inosine
-
mutant Y88A, pH 7.0, 25C
20
-
Inosine
-
-
20
-
Inosine
-
pH 7.4, 25C
25
-
Inosine
-
mutant N123L/R210Q, pH 7.5, 25C
28.3
-
Inosine
-
mutant Y88F, pH 7.0, 25C
31
-
Inosine
-
pH 7.5, 25C
31.5
-
Inosine
-
mutant L22E/H104R, pH 7.4, 25C
32
-
Inosine
-
mutant enzyme F159A
36.4
-
Inosine
-
mutant enzyme N243D/K244Q, pH 6.0
38
-
Inosine
-
mutant V39T/N123L/R210Q, pH 7.5, 25C
38.9
-
Inosine
-
wild-type enzyme, pH 6.0
40
-
Inosine
-
wild-tpe, pH 7.4, 25C
41
-
Inosine
A4Q998, -
-
43.9
-
Inosine
-
wild-type, pH 7.0, 25C
44
-
Inosine
-
wild-type, pH 7.5, 25C
44.93
-
Inosine
-
37C
46
-
Inosine
-
mutant enzyme E89A
47.3
-
Inosine
-
wild-type enzyme, pH 7.0
49
-
Inosine
-
mutant enzyme K244A
52
-
Inosine
-
mutant enzyme M219A
54
-
Inosine
-
mutant enzyme Y88F
56
-
Inosine
-
wild-type enzyme
56
-
Inosine
-
recombinant erythrocyte enzyme
56
-
Inosine
-
pH 7.5, 25C
56.4
-
Inosine
-
mutant T156A, pH 7.6, 37C
62.3
-
Inosine
-
mutant T90S, pH 7.6, 37C
82.4
-
Inosine
-
mutant V206I, pH 7.6, 37C
84.1
-
Inosine
-
wild-type, pH 7.6, 37C
84.1
-
Inosine
-
pH 7.6, 37C
84.19
-
Inosine
-, Q8U2I1
80C
98.4
-
Inosine
-
mutant T156S, pH 7.6, 37C
104.9
-
Inosine
-
50C
113.6
-
Inosine
A3F8V1, -
-
119
-
Inosine
-
-
164.4
-
Inosine
-
mutant T90R, pH 7.6, 37C
173
-
Inosine
Q72IR2
pH 7.0, 80C
324.3
-
Inosine
-
mutant T90R/T156A, pH 7.6, 37C
3900
-
Inosine
-
isoform of 702 amino acids, pH 7.0, 37C
6200
-
Inosine
-
pH 7.0, 37C
7000
-
Inosine
-
pH 7.0, 37C
12000
-
Inosine
-
isoform of 816 amino acids, pH 7.0, 37C
28000
-
Inosine
-, Q9KCN8
pH 7.5
0.2
-
Methylthioinosine
-
pH 7.5, 25C
1.5
-
Methylthioinosine
-
pH 7.5, 25C
2.6
-
Methylthioinosine
-
pH 7.4
0.001
-
p-nitrophenyl beta-D-ribofuranoside
-
25C, pH 7.4
1
-
p-nitrophenyl beta-D-ribofuranoside
-
-
0.09
-
phosphate
-
reaction with inosine, mutant enzyme N243A
1.1
-
phosphate
-
reaction with inosine, mutant enzyme K244A
1.5
-
phosphate
-
reaction with inosine, mutant enzyme R84A
1.8
-
phosphate
-
reaction with inosine, mutant enzyme E201A
2.01
-
phosphate
-
reaction with 7-methylinosine
3.5
-
phosphate
-
cosubstrate guanosine, pH 7.0, 25C
4.9
-
phosphate
-
cosubstrate 2'-deoxyguanosine, pH 7.0, 25C; cosubstrate inosine, pH 7.0, 25C
6.2
-
phosphate
-
reaction with inosine, mutant enzyme F200A
6.9
-
phosphate
-
cosubstrate 2'-deoxyinosine, pH 7.0, 25C
9.4
-
phosphate
-
reaction with inosine, mutant enzyme H86A
12
-
phosphate
-
reaction with inosine, mutant enzyme T242A
13
-
phosphate
-
reaction with inosine, mutant enzyme H257A
13.3
-
phosphate
-
cosubstrate 7-methylinosine, pH 7.0, 25C
17.9
-
phosphate
-
cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25C
27.8
-
phosphate
-
reaction with 7-methylinosine
30
-
phosphate
-
reaction with inosine, mutant enzyme F159A
41
-
phosphate
-
reaction with inosine, mutant enzyme M219A
44.93
-
phosphate
-
37C
52
-
phosphate
-
reaction with inosine, mutant enzyme K244A
53
-
phosphate
-
reaction with inosine, mutant enzyme E89A
56
-
phosphate
-
reaction with inosine, mutant enzyme Y88F; wild-type enzyme
56
-
phosphate
-
recombinant erythrocyte enzyme
104.9
-
phosphate
-
50C
113.6
-
phosphate
A3F8V1, -
-
0.09
-
uridine
-
pH 7.4
4
-
Xanthosine
-
-
3.4
-
Methylthioinosine
-
pH 7.4
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0009
-
2',3'-dideoxyinosine
-
mutant Y88E, pH 7.0, 25C
1604
0.0018
-
2',3'-dideoxyinosine
-
mutant Y88D, pH 7.0, 25C
1604
0.044
-
2',3'-dideoxyinosine
-
mutant Y88T, pH 7.0, 25C
1604
0.091
-
2',3'-dideoxyinosine
-
mutant Y88W, pH 7.0, 25C
1604
0.115
-
2',3'-dideoxyinosine
-
mutant Y88I, pH 7.0, 25C
1604
0.144
-
2',3'-dideoxyinosine
-
mutant Y88V, pH 7.0, 25C
1604
0.153
-
2',3'-dideoxyinosine
-
mutant Y88M, pH 7.0, 25C
1604
0.177
-
2',3'-dideoxyinosine
-
mutant Y88Q, pH 7.0, 25C
1604
0.652
-
2',3'-dideoxyinosine
-
mutant Y88S, pH 7.0, 25C
1604
0.875
-
2',3'-dideoxyinosine
-
wild-type, pH 7.0, 25C
1604
1.67
-
2',3'-dideoxyinosine
-
mutant Y88A, pH 7.0, 25C
1604
3.07
-
2',3'-dideoxyinosine
-
mutant Y88C, pH 7.0, 25C
1604
3.67
-
2',3'-dideoxyinosine
-
mutant Y88H, pH 7.0, 25C
1604
8.09
-
2',3'-dideoxyinosine
-
mutant Y88M, pH 7.0, 25C
1604
15.67
-
2',3'-dideoxyinosine
-
mutant Y88L, pH 7.0, 25C
1604
23.14
-
2',3'-dideoxyinosine
-
mutant Y88F, pH 7.0, 25C
1604
340
-
2'-deoxyguanosine
-
pH 7.0, 25C
1647
822.5
-
2'-deoxyguanosine
Q72IR2
pH 7.0, 80C
1647
9.9
-
2'-deoxyinosine
-
pH 7.4
1648
15.2
-
2'-deoxyinosine
-
pH 7.4
1648
110
-
2'-deoxyinosine
-
pH 7.0, 25C
1648
304.3
-
2'-deoxyinosine
Q72IR2
pH 7.0, 80C
1648
600
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
-
138014
568
-
2-fluoro-2'-deoxyadenosine
-
mutant E201Q/N243D
221829
240
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
5878
488
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
5878
1548
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
5878
1674
-
7-methylguanosine
-
presence of 100 mM phosphate, pH 7.0
5878
2256
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
5878
5860
-
7-methylguanosine
-
presence of 50 mM phosphate, pH 7.0
5878
550
-
7-Methylinosine
-
pH 7.0, 25C
5881
58.7
-
guanosine
-, Q9KCN8
pH 7.5
11207
289
-
guanosine
-
pH 7.4
11207
330
-
guanosine
-
pH 7.0, 25C
11207
550
-
guanosine
-
pH 7.4, 25C
11207
558.8
-
guanosine
Q72IR2
pH 7.0, 80C
11207
630
-
guanosine
-
mutant L22E/H104R, pH 7.4, 25C
11207
730
-
guanosine
-
wild-tpe, pH 7.4, 25C
11207
1020
-
guanosine
-
mutant E201Q/N243D
11207
4269
-
guanosine
-
pH 7.4
11207
0.00014
-
Inosine
-
mutant Y88E, pH 7.0, 25C
11674
0.00029
-
Inosine
-
mutant Y88D, pH 7.0, 25C
11674
0.0009
-
Inosine
-
mutant Y88I, pH 7.0, 25C
11674
0.0017
-
Inosine
-
mutant Y88Q, pH 7.0, 25C
11674
0.0019
-
Inosine
-
mutant Y88T, pH 7.0, 25C; mutant Y88V, pH 7.0, 25C
11674
0.0114
-
Inosine
-
mutant Y88M, pH 7.0, 25C
11674
0.013
-
Inosine
-
mutant Y88W, pH 7.0, 25C
11674
0.0203
-
Inosine
-
mutant Y88S, pH 7.0, 25C
11674
0.0321
-
Inosine
-
mutant Y88C, pH 7.0, 25C
11674
0.0371
-
Inosine
-
mutant Y88M, pH 7.0, 25C
11674
0.082
-
Inosine
-
mutant T90A/T156A, pH 7.6, 37C
11674
0.087
-
Inosine
-
mutant T90A, pH 7.6, 37C
11674
0.116
-
Inosine
-
mutant Y88H, pH 7.0, 25C
11674
0.169
-
Inosine
-
mutant Y88L, pH 7.0, 25C
11674
0.219
-
Inosine
-
mutant Y88A, pH 7.0, 25C
11674
0.389
-
Inosine
-
mutant Y88F, pH 7.0, 25C
11674
0.921
-
Inosine
-
wild-type, pH 7.0, 25C
11674
41.8
-
Inosine
-
mutant T156A, pH 7.6, 37C
11674
67.8
-
Inosine
-
mutant T90S, pH 7.6, 37C
11674
74
-
Inosine
Q8I3X4
pH 7.5, 25C
11674
83.2
-
Inosine
Q72IR2
pH 7.0, 80C
11674
140
-
Inosine
-
pH 7.0, 25C
11674
189
-
Inosine
-
mutant V206I, pH 7.6, 37C
11674
215
-
Inosine
-
wild-type, pH 7.6, 37C
11674
215
-
Inosine
-
pH 7.6, 37C
11674
234
-
Inosine
-
pH 7.4
11674
600
-
Inosine
-
mutant N123L/R210Q, pH 7.5, 25C
11674
1000
-
Inosine
-
mutant V39T/N123L/R210Q, pH 7.5, 25C
11674
1200
-
Inosine
-
mutant L22E/H104R, pH 7.4, 25C
11674
1250
-
Inosine
-
pH 7.4, 25C
11674
1300
-
Inosine
-
wild-tpe, pH 7.4, 25C
11674
1400
-
Inosine
-
wild-type, pH 7.5, 25C
11674
1400
-
Inosine
-
pH 7.5, 25C
11674
3540
-
Inosine
-
mutant T156S, pH 7.6, 37C
11674
4069
-
Inosine
-
pH 7.4
11674
11500
-
Inosine
-
mutant T90R, pH 7.6, 37C
11674
43500
-
Inosine
-
mutant T90R/T156A, pH 7.6, 37C
11674
74000
-
Inosine
-
pH 7.0, 37C
11674
120000
-
Inosine
-, Q9KCN8
pH 7.5
11674
160000
-
Inosine
-
pH 7.0, 37C
11674
320000
-
Inosine
-
isoform of 702 amino acids, pH 7.0, 37C
11674
3900000
-
Inosine
-
isoform of 816 amino acids, pH 7.0, 37C
11674
240.7
-
Methylthioinosine
-
pH 7.4
13157
6800
-
Methylthioinosine
-
pH 7.4
13157
2.1
-
phosphate
-
cosubstrate 2'-deoxyguanosine, pH 7.0, 25C
27500
2.6
-
phosphate
-
cosubstrate guanosine, pH 7.0, 25C
27500
6.1
-
phosphate
-
cosubstrate 2'-deoxyinosine, pH 7.0, 25C
27500
31
-
phosphate
-
cosubstrate 7-methylinosine, pH 7.0, 25C
27500
42
-
phosphate
-
cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25C
27500
8400
-
phosphate
-
cosubstrate inosine, pH 7.0, 25C
27500
0.306
-
uridine
Q8I3X4
pH 7.5, 25C
17761
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1e-06
-
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
approximate value, with (hypoxanthine-9-yl)methyl aglycone, i.e. Yokomatsu compound
-
7.2e-06
-
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranyl)ethylphosphonic acid with (hypoxanthine-9-yl)methyl aglycone
-
erythrocyte enzyme, pH 7.0, 25C
1.12e-05
-
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranyl)ethylphosphonic acid with (hypoxanthine-9-yl)methyl aglycone
-
pH 7.0, 25C
1.82e-06
-
(3R,4R)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
overall dissociation constant
0.01
-
(3S,4R)-4-(guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylcarbonylphosphonic acid
-
recombinant enzyme, pH 7.5, temperature not specified in the publication
3.2e-08
-
(3S,4S)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
overall dissociation constant
0.02
-
(S)-3-(guanin-9-yl)pyrrolidin-N-ylcarbonylphosphonic acid
-
recombinant enzyme, pH 7.5, temperature not specified in the publication
0.025
-
1,6-Dihydropurine riboside
-
-
0.027
-
1-((2-pyrrolidine-1-yl)ethyl)uracil
Q8I3X4
pH 7.5, 25C
0.0021
-
1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine
-
reaction with 7-methylguanosine
0.0062
-
1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine
-
reaction with inosine
0.02
-
1-methylguanine
-
-
0.000306
-
2-amino-1,5-dihydro-7-[[(2S)-2-(aminomethyl)-1-pyrrolidinyl]-methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
37C, pH 7.0
2e-06
-
2-amino-1,5-dihydro-7-[[(2S)-2-(hydroxymethyl)-1-pyrrolidinyl]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
37C, pH 7.0, acetic acid salt
1.02e-05
-
2-amino-1,5-dihydro-7-[[[2-(hydroxy)ethyl]amino]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
37C, pH 7.0
0.0024
-
2-amino-6-chloro-7-deazapurine 2'-deoxyriboside
-
-
0.038
-
2-amino-6-methyl-5,6,7,8-tetrahydropteridin-4(1H)-one
-
-
-
0.3
-
2-Amino-6-methylthiopurine
-
-
3.4e-09
-
2-amino-7-([[(2R,3S)-1,3,4-trihydroxybutan-2-yl]amino]methyl)-3,5-dihydro-4Hpyrrolo[3,2-d]pyrimidin-4-one
-
-
2.1e-09
-
2-amino-7-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
3.06e-05
-
2-amino-7-[[(2-hydroxyethyl)(methyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0003163
-
2-amino-7-[[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0003061
-
2-amino-7-[[(2S)-2-(methoxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
1e-06
-
2-amino-7-[[(2S,4R)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
1.8e-06
-
2-amino-7-[[3-(hydroxymethyl)piperidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
2.5e-06
-
2-amino-7-[[bis(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0001122
-
2-amino-7-[[ethyl(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0014
-
2-chloro-6-(3-phenyl-1-propoxy)purine
-
-
0.006
-
3-((2-pyrrolidine-1-yl)ethyl)uracil
Q8I3X4
pH 7.5, 25C
1.5e-06
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
; 25C, pH 7.4
1.8e-05
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-G
-
-
1.07e-08
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
25C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase
1.1e-08
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
overall dissociation constant
2.7e-07
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
25C, pH 7.4, mutant enzyme H257G, measured in slow-onset tight-binding phase
9e-07
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
25C, pH 7.4, mutant enzyme H257D, measured in slow-onset tight-binding phase
9.5e-07
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
25C, pH 7.4, mutant enzyme H257F, measured in slow-onset tight-binding phase
3.8e-06
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
; 25C, pH 7.4
1.8e-05
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
25C, pH 7.4
0.0004
-
5'-chloro-5'-deoxy-8-aminoguanosine
-
-
0.012
-
5'-chloro-5'-deoxy-8-aminoguanosine
-
-
0.00018
-
5'-deoxy-5'-iodo-9-deazainosine
-
-
0.018
-
5'-deoxy-5'-iodoinosine
-
-
7.7e-09
-
5'-deoxy-immucillin-H
-
25C, pH 7.4, mutant enzyme H257G
2e-08
-
5'-deoxy-immucillin-H
-
25C, pH 7.4, wild-type enzyme
5.4e-08
-
5'-deoxy-immucillin-H
-
25C, pH 7.4, mutant enzyme H257D
1.98e-07
-
5'-deoxy-immucillin-H
-
25C, pH 7.4, mutant enzyme H257F
2.7e-06
-
5'-methylthio-immucillin-H
-
pH 7.4, equilibrium inhibition constant
-
0.008
-
6-amino-2-chloro-7-deazapurine 2'-deoxyriboside
-
-
0.005
-
6-amino-7-phenylethinyl-7-deazapurine 2'-deoxyriboside
-
-
0.0006
-
6-benzyloxy-2-chloropurine
-
-
0.0023
-
6-chloro-7-deaza-purine 2'-deoxyriboside
-
-
0.203
-
6-hydroxy-9-p-aminobenzylpurine
-
-
0.073
-
6-Mercaptopurine
-
-
0.005
-
6-methylformycin A
-
pH 6.0
-
0.0252
-
6-methylformycin A
-
pH 5.5
-
0.13
-
6-methylthiopurine
-
-
1.1
-
6-Thioxanthine
-
-
0.2
-
7-Deazaguanine
-
pH 7.6, 25C
0.06
-
7-Deazainosine
-
-
0.085
-
7-Deazainosine
-
-
0.33
-
7-Deazainosine
-
-
0.002
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 7.3, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM
0.0028
0.0035
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 6.5, concentration of the substrate 8-azaguanosine is 0.05-0.25 mM
0.0036
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 7.9, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM
0.0037
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 8.2, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM
0.0042
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 8.2, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM
0.038
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 6.0, concentration of the substrate 7-methylguanosine is 0.032 mM
0.054
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 7.0, concentration of the substrate 7-methylguanosine is 0.03 mM
0.073
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 7.0, concentration of the substrate 7-methylguanosine is 0.122 mM
0.093
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 8.0, concentration of the substrate 7-methylguanosine is 0.032 mM
0.082
-
7-methylguanine
P81989
25C, pH 7.0, with 7-methylguanosine as varied substrate
1.6e-08
-
7-[[(3R,4R)-4-hydroxy-3-(hydroxymethyl)pyrrolidin-2-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0033
-
8-amino-5'-deoxy-5'-iodoguanosine
-
-
0.0002
-
8-Amino-9-benzylguanine
-
-
0.008
-
8-Amino-9-benzylguanine
-
-
0.0002
-
8-aminoguanine
-
-
0.007
-
8-aminoguanosine
-
-
0.013
-
8-aminoguanosine
-
-
0.017
-
8-aminoguanosine
-
-
0.195
-
8-aza-2,6-diaminopurine
-
-
0.0041
-
8-bromo-N(9)-acycloguanosine
-
-
0.024
-
9-(1,3-dihydroxy-2-propoxymethyl)guanine
-
reaction with 7-methylguanosine
0.027
-
9-(1,3-dihydroxy-2-propoxymethyl)guanine
-
reaction with inosine
7.5e-06
-
9-(2'-benzyl-5',5'-difluoro-5'-phosphonopentyl)guanine
-
pH 7.0, 25C
7.6e-06
-
9-(2'-benzyl-5',5'-difluoro-5'-phosphonopentyl)guanine
-
erythrocyte enzyme, pH 7.0, 25C
0.058
-
9-(2-hydroxyethoxymethyl)guanine
-
reaction with 7-methylguanosine
0.068
-
9-(2-hydroxyethoxymethyl)guanine
-
reaction with inosine
4.4e-06
-
9-(5',5'-difluoro-5'-phosphonobutyl)-9-deazaguanine
-
pH 7.0, 25C
8.1e-06
-
9-(5',5'-difluoro-5'-phosphonobutyl)-9-deazaguanine
-
erythrocyte enzyme, pH 7.0, 25C
5.3e-06
-
9-(5',5'-difluoro-5'-phosphonoheptyl)-9-deazaguanine
-
erythrocyte enzyme, pH 7.0, 25C
5.7e-06
-
9-(5',5'-difluoro-5'-phosphonoheptyl)-9-deazaguanine
-
pH 7.0, 25C
3.3e-06
-
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
pH 7.0, 25C, in the presence of 1 mM phosphate
0.0069
-
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
wild-type, pH not specified in the publication, temperature not specified in the publication
2e-06
-
9-(5,5-difluoro-5-phosphonopentyl)guanine
-
approximate value
5.6e-06
-
9-deazaguanine
-
erythrocyte enzyme, pH 7.0, 25C
6.1e-06
-
9-deazaguanine
-
pH 7.0, 25C
0.09
-
acyclovir
-
-
0.11
-
acyclovir
-
-
6.6
-
adenine
-
-
0.049
-
adenosine
-
-
0.16
-
adenosine
-
-
0.97
-
allopurinol
-
-
0.0085
-
alpha-D-ribose 1-phosphate
-
with guanosine as variable substrate
0.035
-
alpha-D-ribose 1-phosphate
-
with phosphate as variable substrate
0.32
-
alpha-D-ribose 1-phosphate
-
with phosphate as variable substrate and 0.2 mM guanosine
0.36
-
alpha-D-ribose 1-phosphate
-
with guanosine as variable substrate and 20 mM phosphate
0.361
-
alpha-D-ribose 1-phosphate
-
-
0.15
-
aminopterin
-
-
0.063
-
cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
6.6e-08
-
cis-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethylpyrrolidin-3-ol
-
overall dissociation constant
3.4e-08
-
DADMe-immucillin-G
-
wild-tpe, pH 7.4, 25C
1.21e-07
-
DADMe-immucillin-G
-
mutant L22E/H104R, pH 7.4, 25C
2.4e-07
-
DADMe-immucillin-H
-
mutant L22E/H104R, pH 7.4, 25C
3.5e-07
-
DADMe-immucillin-H
-
wild-tpe, pH 7.4, 25C
7.4e-06
-
deoxyimmucillin-H
-
pH 7.4, equilibrium inhibition constant
1e-05
-
deoxyimmucillin-H
Q2HXR2
pH 7.4, equilibrium inhibition constant
0.00013
-
deoxyimmucillin-H
-
pH 7.4, inhibition constant during slow-onset phase
0.000682
-
deoxyimmucillin-H
Q2HXR2
pH 7.4, inhibition constant during slow-onset phase
1.5
-
Deoxyinosine
-
-
0.33
-
diphosphate
-
-
0.19
-
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
0.0337
-
folic acid
-
-
0.005
-
formycin
-
-
0.0053
-
Formycin A
-
-
0.0053
-
Formycin A
-
pH 7.0
0.0055
-
Formycin A
-
-
0.0138
-
Formycin A
-
pH 5.5
0.062
-
Formycin A
-
-
0.0685
-
Formycin A
-
mutant enzyme N243D/K244Q, with adenosine as substrate
0.114
-
Formycin A
-
mutant enzyme N243D/K244Q, with inosine as substrate
0.143
-
Formycin A
-
mutant enzyme N243D, with adenosine as substrate
0.667
-
Formycin A
-
wild-type enzyme, with inosine as substrate
0.00039
-
formycin B
-
-
0.0011
-
formycin B
-
-
0.0045
-
formycin B
-
-
0.0046
-
formycin B
-
pH 7
0.0046
-
formycin B
-
-
0.019
-
formycin B
-
-
0.1
-
formycin B
-
-
0.1
-
formycin B
-
inhibition of phosphorolysis of 7-methylguanosine; phosphorolysis of 7-methylguanosine
0.13
-
formycin B
-
-
0.279
-
formycin B
-
wild-type enzyme, with inosine as substrate
0.339
-
formycin B
-
mutant enzyme N243D/K244Q, with inosine as substrate
0.5
-
formycin B
-
-
0.67
-
formycin B
-
mutant enzyme N243D, with inosine as substrate
0.705
-
formycin B
-
mutant enzyme N243D/K244Q, with inosine as substrate
0.0025
-
Guanine
P81989
25C, pH 7.0, with phosphate as varied substrate
0.0025
-
Guanine
-
versus phosphate
0.0025
-
Guanine
-
pH 7.0, 25C
0.0036
-
Guanine
-
-
0.0036
-
Guanine
P81989
25C, pH 7.0, with inosine as varied substrate
0.0036
-
Guanine
-
versus inosine
0.004
-
Guanine
-
inhibition of phosphorolysis of xanthosine
0.005
-
Guanine
-
-
0.0065
-
Guanine
-
with guanosine as variable substrate
0.012
-
Guanine
-
with guanosine as variable substrate and 200 mM phosphate
0.0125
-
Guanine
-
with guanosine as variable substrate and phosphate as fixed substrate
0.0125
-
Guanine
-
-
0.0145
-
Guanine
-
with guanosine as variable substrate and 40 mM phosphate
0.025
-
Guanine
-
with phosphate as variable substrate
0.065
-
Guanine
-
with phosphate as variable substrate and 0.2 mM guanosine
0.01
-
guanosine
-
with guanine as variable substrate and ribose 1-phosphate as fixed substrate
0.01
-
guanosine
-
inhibition of phosphorolysis of xanthosine
0.0313
-
guanosine
-
with guanine as variable substrate and 0.4 mM ribose 1-phosphate
0.8
-
guanosine
P81989
25C, pH 7.0, about, with alpha-D-ribose 1-phosphate as varied substrate
0.8
-
guanosine
-
versus alpha-D-ribose 1-phosphate
0.0016
-
hypoxanthine
-
pH 7.0, 25C
0.007
-
hypoxanthine
-
inhibition of phosphorolysis of xanthosine
0.01
-
hypoxanthine
-
-
0.025
-
hypoxanthine
-
-
0.05
-
hypoxanthine
-
with guanosine as variable substrate and 200 mM phosphate
1e-06
-
immucillin-A
-
; 25C, pH 7.4
1.33e-07
-
immucillin-G
-
wild-tpe, pH 7.4, 25C
1.75e-07
-
immucillin-G
-
mutant L22E/H104R, pH 7.4, 25C
9e-07
-
immucillin-G
-
pH 7.4, equilibrium inhibition constant
1.89e-06
-
immucillin-G
Q2HXR2
pH 7.4, equilibrium inhibition constant
1.6e-05
-
immucillin-G
-
; 25C, pH 7.4
0.000346
-
immucillin-G
Q2HXR2
pH 7.4, inhibition constant during slow-onset phase
1.1e-08
-
immucillin-H
-
25C, pH 7.4, mutant enzyme H257G
5.79e-08
-
immucillin-H
-
25C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase
7.2e-08
-
immucillin-H
-
-
8.6e-08
-
immucillin-H
-
25C, pH 7.4, mutant enzyme H257D
1.72e-07
-
immucillin-H
-
25C, pH 7.4, mutant enzyme H257F
6e-07
-
immucillin-H
-
pH 7.4, equilibrium inhibition constant
6.7e-07
-
immucillin-H
-
mutant L22E/H104R, pH 7.4, 25C
1e-06
-
immucillin-H
-
wild-tpe, pH 7.4, 25C
2.03e-06
-
immucillin-H
Q2HXR2
pH 7.4, equilibrium inhibition constant
1.2e-05
-
immucillin-H
-
; 25C, pH 7.4
2.9e-05
-
immucillin-H
-
pH 7.4, inhibition constant during slow-onset phase
0.00045
-
immucillin-H
Q2HXR2
pH 7.4, inhibition constant during slow-onset phase
0.26
-
Inosine
-
-
0.027
-
N(1)-Methylformycin A
-
pH 7
0.00027
-
N(6)-methyl-formycin A
-
pH 8.0
0.0003
-
N(6)-methyl-formycin A
-
pH 7
0.0003
-
N(6)-methyl-formycin A
-
-
0.018
-
N(6)-methylformycin B
-
pH 7
0.005
-
N(7)-acycloguanosine
-
-
0.097
-
N(7)-acycloguanosine
-
-
0.0022
-
N(7)-methylformycin A
-
pH 5.5
0.0034
-
N(9)-acycloguanosine
-
-
0.014
-
N(9)-acycloguanosine
-
-
0.81
-
Oxoallopurinol
-
-
0.2
-
oxoformycin A
-
pH 7
-
0.18
-
oxoformycin B
-
pH 7
2.5
-
phosphate
P81989
25C, pH 7.0, with alpha-D-ribose 1-phosphate as varied substrate
4.4
-
phosphate
-
with ribose 1-phosphate as variable substrate and guanine as fixed substrate
4.7
-
phosphate
P81989
25C, pH 7.0, with guanine as varied substrate
4.7
-
phosphate
-
versus guanine
26
-
phosphate
-
with guanine as variable substrate and ribose 1-phosphate as fixed substrate
0.49
-
purine riboside
-
-
0.323
-
quinazolinone
-
-
3.8e-07
-
L-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
slow-onset binding constant
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.6e-05
-
(3S)-3-(2-amino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-(3-chlorophenyl)propanoic acid
-
IC50: 0.000036 mM
6.4e-06
-
2'-deoxy-immucillin-G
Q8T9Z7
gene disruption mutant, pH 7.4
0.00053
-
2'-deoxy-immucillin-G
Q8T9Z7
wild-type, pH 7.4
1.6e-05
-
2'-deoxy-immucillin-H
Q8T9Z7
gene disruption mutant, pH 7.4
0.00044
-
2'-deoxy-immucillin-H
Q8T9Z7
wild-type, pH 7.4
5e-05
-
2,6-diamino-7-(5-mercapto-3,3-dimethylpentyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00005 mM
0.0066
-
2,6-diamino-7-(cyclohexylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.0066 mM
0.00849
-
2,8-diamino-1,7-dihydro-6H-purine-6-thione
-
IC50: 0.00849 mM
0.002299
-
2,8-diamino-1,9-dihydro-6H-purin-6-one
-
IC50: 0.002299 mM
0.001758
-
2,8-diamino-3-(2-thienylmethyl)-3,9-dihydro-6H-purin-6-one
-
IC50: 0.001758 mM
0.001043
-
2,8-diamino-9-(1,3-thiazol-4-ylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.001043 mM
9.2e-05
-
2,8-diamino-9-(2-furylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000092 mM
0.00271
-
2,8-diamino-9-(2-furylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.00271 mM
0.000497
-
2,8-diamino-9-(2-thienylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000497 mM
0.00417
-
2,8-diamino-9-(4-fluorobenzyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.00417 mM
0.001971
-
2,8-diamino-9-(pyridin-2-ylmethyl)-1,9-dihydro-6H-purin-6-one
-
IC50: 0.001971 mM
0.000362
-
2,8-diamino-9-[(4-methyl-2-thienyl)methyl]-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000362 mM
0.000852
-
2,8-diamino-9-[4-(1H-imidazol-1-yl)benzyl]-1,9-dihydro-6H-purin-6-one
-
IC50: 0.000852 mM
0.00152
-
2,8-diamino-9-[[1-(heptyloxy)-2-hydroxyethoxy]methyl]-1,9-dihydro-6H-purin-6-one
-
IC50: 0.00152 mM
0.00703
-
2-(3,4-dichlorophenyl)-5-thioxo-5,6-dihydro-1H-pyrazolo[4,3-d]pyrimidine-3,7(2H,4H)-dione
-
IC50: 0.00703 mM
0.0006
-
2-amino-1,5-dihydro-7-[[(2S)-2-(aminomethyl)-1-pyrrolidinyl]-methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 600 nM
4e-06
-
2-amino-1,5-dihydro-7-[[(2S)-2-(hydroxymethyl)-1-pyrrolidinyl]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
acetic acid salt, IC50: 4.0 nM
2e-05
-
2-amino-1,5-dihydro-7-[[[2-(hydroxy)ethyl]amino]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 20 nM
1.8e-05
-
2-amino-7-(1,2-dithian-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000018 mM
0.00024
-
2-amino-7-(2,3,5-trichlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00024 mM
0.00012
-
2-amino-7-(2-chlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00012 mM
8.3e-05
-
2-amino-7-(2-furylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000083 mM
0.00027
-
2-amino-7-(2-hydroxybenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00027 mM
2.1e-05
-
2-amino-7-(2-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000021 mM
0.000674
-
2-amino-7-(2-thienylmethyl)-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one
-
IC50: 0.000674 mM
1.7e-05
-
2-amino-7-(3,4-dichlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000017 mM
2e-05
-
2-amino-7-(3-chlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00002 mM
2.4e-05
-
2-amino-7-(3-fluorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000024 mM
7e-05
-
2-amino-7-(3-hydroxybenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00007 mM
8.2e-05
-
2-amino-7-(3-methoxybenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000082 mM
5.7e-05
-
2-amino-7-(3-methylbenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000057mM
2.5e-05
-
2-amino-7-(3-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2.5e-05
-
2-amino-7-(4-chlorobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2.3e-05
-
2-amino-7-(4-iodobenzyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000023 mM
0.000546
-
2-amino-7-(biphenyl-4-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000546 mM
3e-05
-
2-amino-7-(cycloheptylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00003 mM
4.7e-05
-
2-amino-7-(cyclohexylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000047 mM
2.9e-05
-
2-amino-7-(cyclopentylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000029 mM
0.001
-
2-amino-7-(piperidin-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.001 mM
1.5e-05
-
2-amino-7-(pyridin-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000015 mM
2.3e-05
-
2-amino-7-(pyridin-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
pH 7.4, 25C
0.007
-
2-amino-7-(pyridin-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
pH 7.4, 25C
2.5e-05
-
2-amino-7-(pyridin-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
6.4e-05
-
2-amino-7-(pyridin-4-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000064 mM
1.1e-05
-
2-amino-7-(tetrahydro-2-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000011 mM
7e-05
-
2-amino-7-(tetrahydrofuran-2-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00007 mM
5.1e-05
-
2-amino-7-benzyl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000051 mM
0.0019
-
2-amino-7-cyclohex-1-en-1-yl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.0019 mM
4.2e-05
-
2-amino-7-phenoxy-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000042 mM
2.5e-05
-
2-amino-7-[(3-methylcyclohexyl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
2.5e-05
-
2-amino-7-[(3-tert-butylcyclohexyl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000025 mM
0.000135
-
2-amino-7-[(4-chloropyridin-3-yl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000135 mM
0.000124
-
2-amino-7-[3-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000124 mM
0.000147
-
2-amino-7-[3-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000147 mM
3.6e-05
-
2-amino-7-[3-(trifluoromethyl)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000036 mM
0.0003
-
2-amino-7-[4-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
pH 7.4, 25C
0.0005
-
2-amino-7-[4-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
pH 7.4, 25C
0.00015
-
2-amino-7-[4-(propan-2-yl)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
pH 7.4, 25C
0.00027
-
2-amino-7-[4-(propan-2-yl)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
pH 7.4, 25C
9e-05
-
2-amino-7-[[(1R,3S,5S,7S)-4-methyl-2-adamantyl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00009 mM
6e-05
-
2-amino-7-[[(2-hydroxyethyl)(methyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 60 nM
0.00062
-
2-amino-7-[[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 620 nM
0.0006
-
2-amino-7-[[(2S)-2-(methoxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 600 nM
2e-06
-
2-amino-7-[[(2S,4R)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 2 nM
0.004
-
2-amino-7-[[3-(hydroxymethyl)piperidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.004 mM
5e-06
-
2-amino-7-[[bis(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 5 nM
0.00022
-
2-amino-7-[[ethyl(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 220 nM
0.002866
-
2-amino-9-(2-thienylmethyl)-8-thioxo-1,7,8,9-tetrahydro-6H-purin-6-one
-
IC50: 0.002866 mM
0.000106
8.4e-05
2-fluoro-2'-deoxyadenosine
-
the substrate releases highly cytotoxic 2-fluoroadenine. 2-Fluoro-2'-deoxyadenosine and 2-fluoroadenine exert strong inhibition of Trichomonas vaginalis growth with estimated IC50 values of 106 nM and 84 nM. 2-Fluoro-2'-deoxyadenosine might be useful as a
0.02284
-
2-phenyl-5,7-dithioxo-1,2,4,5,6,7-hexahydro-3H-pyrazolo[4,3-d]pyrimidin-3-one
-
IC50: 0.02284 mM
0.27
-
3,5-bis-(4-chlorobenzoyl)-alpha-D-ribose 1-phosphate
-
pH 7.6, 37C
-
0.372
-
3,5-bis-(4-chlorobenzoyl)-alpha-D-ribose 1-phosphate
-
pH 7.6, 37C
-
4.7e-05
-
3-(2-amino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-phenylpropanoic acid
-
IC50: 0.000047 mM
1.1e-05
-
3-(3-chlorophenyl)-3-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)propanenitrile
-
IC50: 0.000011 mM
1e-05
-
3-(3-chlorophenyl)-3-(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)propanenitrile
-
IC50: 0.00001 mM
3.9e-05
-
3-(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-pyridin-3-ylpropanenitrile
-
IC50: 0.000039 mM
0.047
-
3-[(2R,5S)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-3,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one
-
pH 7.4, 25C
0.3
-
3-[(2R,5S)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-3,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one
-
pH 7.4, 25C
8.6e-05
-
5'-methylthio-immucillin-H
Q8T9Z7
wild-type, pH 7.4
-
0.0009
-
5'-methylthio-immucillin-H
Q8T9Z7
gene disruption mutant, pH 7.4
-
0.0001
-
5-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3,3-dimethylpentane-1-sulfonamide
-
IC50: 0.0001 mM
0.001839
-
5-amino-3-(2-thienylmethyl)-3,6-dihydro-7H-[1,2,3]triazolo[4,5-d]pyrimidin-7-one
-
IC50: 0.001839 mM
0.2
-
6-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-4,4-dimethylhexanamide
-
IC50: 0.2 mM
0.00026
-
6-(2,6-diamino-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-4,4-dimethylhexanenitrile
-
IC50: 0.00026 mM
0.0013
-
6-amino-5-bromopyrimidine-2,4(1H,3H)-dione
-
pH 7.0
0.00016
-
6-amino-7-(2-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00016 mM
2.8e-05
-
7-(3-thienylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000028 mM
2.9e-05
-
7-(cyclopentylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000029 mM
4e-05
-
7-(pyridin-3-ylmethyl)-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00004 mM
3.5e-05
-
7-benzyl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.000035 mM
0.098
-
7-ketopyrazolo[4,3-d]pyrimidine
-
pH 7.0, concentration of the substrate 7-methylguanosine is 0.03 mM; pH 7.0, concentration of the substrate 7-methylguanosine is 0.122 mM
0.00019
-
7-[3-(benzyloxy)benzyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
IC50: 0.00019 mM
0.002
0.004
7KPP
-
synthesis of 8-azaguanosine from 8-azaguanine
0.035
0.1
7KPP
-
phosphorolysis of 7-methylguanosine
0.082
-
adenosine
-
pH 7.0
8.5e-06
-
immucillin-H
Q8T9Z7
gene disruption mutant, pH 7.4
4.3e-05
-
immucillin-H
Q8T9Z7
wild-type, pH 7.4
additional information
-
additional information
-
IC50 values of compounds 6-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one, 7-[(2S,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-2-yl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one, and 7-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]-4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidine-6-carboxylic acid are in the range of 100-300 nM
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00075
-
-
substrate adenosine, pH 7.4
0.0141
-
-
-
0.015
-
-
wild-type enzyme, substrate 9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine
0.016
-
Q8T9Z7
substrate 5'-methylthioinosine, pH 7.4
0.0167
-
-
mutant M64V, substrate 9-[beta-D-arabinofuranosyl]-2-fluoro-adenine
0.0179
-
-, Q8U2I1
; 80C, production of hypoxanthine
0.02
-
-
wild-type enzyme, substrate 9-[beta-D-arabinofuranosyl]-2-fluoro-adenine
0.023
-
Q8T9Z7
substrate inosine, pH 7.4
0.0307
-
-
inosine as a variable substrate
0.041
-
-
substrate inosine, mutant R217A, pH 7.0, 25C
0.05
-
-
substrate adenosine, mutant D204A, pH 7.0, 25C
0.06
-
-
substrate guanosine, mutant D204A, pH 7.0, 25C
0.07
-
-
substrate adenosine, mutant D204N, pH 7.0, 25C; substrate guanosine, mutant D204A/R217A, pH 7.0, 25C
0.09
-
-
substrate guanosine, mutant R24A, pH 7.0, 25C; substrate inosine, mutant D204A, pH 7.0, 25C
0.13
-
-
wild-type enzyme, substrate 9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine
0.13
-
-
substrate adenosine, mutant D204A/R217A, pH 7.0, 25C
0.14
-
-
substrate inosine, mutant D204A/R217A, pH 7.0, 25C
0.18
-
-
substrate inosine, mutant R24A, pH 7.0, 25C
0.36
-
-
substrate adenosine, mutant R24A, pH 7.0, 25C; substrate guanosine, mutant R217A, pH 7.0, 25C
0.567
-
-
wild-type enzyme, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
0.95
-
-
substrate 2'-deoxyinosine, pH 7.4
1.17
-
-
substrate 2'-deoxyguanosine, pH 7.4
1.4
-
-
substrate inosine, mutant D204N, pH 7.0, 25C
1.43
-
-
mutant M64V, substrate 9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine
2.1
-
-
substrate guanosine, mutant D204N, pH 7.0, 25C
2.2
-
-
substrate guanosine, pH 7.4
3.47
-
-
substrate inosine, pH 7.4
4
-
-
mutant M64V, substrate 9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine
4.55
-
-
-
6.3
-
-
substrate adenosine, mutant R217A, pH 7.0, 25C
6.4
-
-
substrate 7-methylguanosine, mutant R24A, pH 7.0, 25C
6.9
-
-
substrate 7-methylguanosine, mutant D204N, pH 7.0, 25C
7.3
-
-
wild-type enzyme, substrate 2-fluoro-2'-deoxyadenosine
8.83
-
-
wild-type enzyme, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
9.83
-
-
mutant M64V, substrate 9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine
<