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Ac-WAEW-NH2 peptide + GDP-mannose
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
?
dolichyl D-mannosyl phosphate + ADAMTS-like 1
dolichyl phosphate + C-mannosylated ADAMTS-like 1
-
purified recombinant tagged substrate. The substrate contains in thrombospondin type-1 repeats, two with predicted C-mannosylation sites, C-mannosylation of TSR1
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-
?
dolichyl D-mannosyl phosphate + human punctin-1
dolichyl phosphate + C-mannosylated human punctin-1
-
purified recombinant tagged substrate. C-mannosylation at Trp39 and Trp42 of a thrombospondin type-1 repeat containing the 36WDAWGPWSECSRTC49 sequence, identified by Tandem mass spectrometry (MS/MS) and MS/MS/MS analysis, TSR1 from punctin-1 carries C-mannosylation in close proximity to O-linked fucose
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-
?
dolichyl D-mannosyl phosphate + MIG-21 protein
dolichyl phosphate + C-mannosylated MIG-21 protein
C-mannosylation at a tryptophan residue
-
-
?
dolichyl D-mannosyl phosphate + UNC-5 protein
dolichyl phosphate + C-mannosylated UNC-5 protein
C-mannosylation at a tryptophan residue
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-
?
dolichyl D-mannosyl phosphate + WAKW
dolichyl phosphate + ?
in vitro assay using radiolabeled donor substrate and a synthetic acceptor peptide WAKW, which forms the minimal acceptor substrate
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-
?
UNC5A + GDP-mannose
C-mannosyl-UNC5A + GDP
additional information
?
-
Ac-WAEW-NH2 peptide + GDP-mannose
?
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-
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
?
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-
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
?
-
-
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
?
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-
-
?
UNC5A + GDP-mannose
C-mannosyl-UNC5A + GDP
C-mannosylation of murine UNC5A
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-
?
UNC5A + GDP-mannose
C-mannosyl-UNC5A + GDP
C-mannosylation of murine UNC5A, DPY19L1 can C-mannosylate the first two tryptophans in the WxxWxxWxxC sequence of UNC5A TSRs
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-
?
UNC5A + GDP-mannose
C-mannosyl-UNC5A + GDP
C-mannosylation of murine UNC5A, DPY19L3 mannosylates the third tryptophan of this sequence and might require a WxxC motif
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-
?
additional information
?
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cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19
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-
?
additional information
?
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cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19
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-
?
additional information
?
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C-mannosylation of tryptophan residues is an endoplasmic reticulum localized, DPY-19 enzyme-catalyzed reaction using the lipid-linked glycoside dolichol-phosphate-mannose as the donor substrate
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?
additional information
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C-mannosylation of tryptophan residues is an endoplasmic reticulum localized, DPY-19 enzyme-catalyzed reaction using the lipid-linked glycoside dolichol-phosphate-mannose as the donor substrate
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-
?
additional information
?
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C-mannosylation is initially described as a modification of the first tryptophan in the WxxW consensus sequence, but is also observed on tryptophans that are not part of this motif. In addition to WxxW, WxxC is also considered to be a consensus sequence for C-mannosylation
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additional information
?
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C-mannosylation is initially described as a modification of the first tryptophan in the WxxW consensus sequence, but is also observed on tryptophans that are not part of this motif. In addition to WxxW, WxxC is also considered to be a consensus sequence for C-mannosylation
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additional information
?
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the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide
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additional information
?
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the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide
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additional information
?
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the single Caenorhabditis elegans C-mannosyltransferase DPY-19 mannosylates the first two tryptophans of a WxxWxxW motif
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additional information
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the single Caenorhabditis elegans C-mannosyltransferase DPY-19 mannosylates the first two tryptophans of a WxxWxxW motif
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additional information
?
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protein C-mannosylation is the attachment of alpha-mannopyranose to tryptophan via a C-C linkage. This post-translational modification typically occurs within the sequence motif WXXW, which is frequently present in thrombospondin type-1 repeats (TSRs). TSRs are especially numerous in and a defining feature of the ADAMTS superfamily. Predicted C-mannosylation sites in the ADAMTS superfamily, overview
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?
additional information
?
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analysis of C-mannosylation sites an substrate recognition, mutational analysis, mass spectroscopy, and metabolic labeling, overview
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?
additional information
?
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DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156
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?
additional information
?
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DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156
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?
additional information
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C-mannosylation of protein Rspo1, Rspo1 has two predicted C-mannosylation sites in the thrombospondin type 1 repeat TSR1, substrate structure, overview. Enzyme DPY19L3 selectively modified Rspo1 at residue W156 but not W153 based on mass spectrometry. Purified recombinant Rspo1 protein from the conditioned medium of overexpressing HT1080-Rspo1-MH cells
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?
additional information
?
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C-mannosylation of protein Rspo1, Rspo1 has two predicted C-mannosylation sites in the thrombospondin type 1 repeat TSR1, substrate structure, overview. Enzyme DPY19L3 selectively modified Rspo1 at residue W156 but not W153 based on mass spectrometry. Purified recombinant Rspo1 protein from the conditioned medium of overexpressing HT1080-Rspo1-MH cells
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?
additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
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additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
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additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
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additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
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additional information
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no activity by DPY19L4 isozyme on murine UNC5A
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additional information
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no activity by DPY19L4 isozyme on murine UNC5A
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additional information
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no activity by DPY19L4 isozyme on murine UNC5A
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additional information
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no activity by DPY19L4 isozyme on murine UNC5A
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additional information
?
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the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide
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additional information
?
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the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide
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additional information
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several thrombospondin type 1 repeat (TSR) domain-containing proteins of Plasmodium falciparum can be C-mannosylated in vivo
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additional information
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several thrombospondin type 1 repeat (TSR) domain-containing proteins of Plasmodium falciparum can be C-mannosylated in vivo
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additional information
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the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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additional information
?
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the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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additional information
?
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the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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additional information
?
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the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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dolichyl D-mannosyl phosphate + MIG-21 protein
dolichyl phosphate + C-mannosylated MIG-21 protein
C-mannosylation at a tryptophan residue
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?
dolichyl D-mannosyl phosphate + UNC-5 protein
dolichyl phosphate + C-mannosylated UNC-5 protein
C-mannosylation at a tryptophan residue
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?
additional information
?
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additional information
?
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cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19
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?
additional information
?
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cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19
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?
additional information
?
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C-mannosylation is initially described as a modification of the first tryptophan in the WxxW consensus sequence, but is also observed on tryptophans that are not part of this motif. In addition to WxxW, WxxC is also considered to be a consensus sequence for C-mannosylation
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additional information
?
-
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C-mannosylation is initially described as a modification of the first tryptophan in the WxxW consensus sequence, but is also observed on tryptophans that are not part of this motif. In addition to WxxW, WxxC is also considered to be a consensus sequence for C-mannosylation
-
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additional information
?
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the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide
-
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additional information
?
-
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the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide
-
-
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additional information
?
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protein C-mannosylation is the attachment of alpha-mannopyranose to tryptophan via a C-C linkage. This post-translational modification typically occurs within the sequence motif WXXW, which is frequently present in thrombospondin type-1 repeats (TSRs). TSRs are especially numerous in and a defining feature of the ADAMTS superfamily. Predicted C-mannosylation sites in the ADAMTS superfamily, overview
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-
?
additional information
?
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DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156
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?
additional information
?
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DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156
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?
additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
-
-
-
additional information
?
-
mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
-
-
-
additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
-
-
-
additional information
?
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mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities
-
-
-
additional information
?
-
the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide
-
-
-
additional information
?
-
-
the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide
-
-
-
additional information
?
-
the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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additional information
?
-
-
the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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-
additional information
?
-
the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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-
additional information
?
-
the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated
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evolution
DPY-19 exhibits topological and sequential homology to the N-glycan oligosaccharyltransferase, highlighting an evolutionary link between N- and C-glycosylation. The enzyme is related to the OST family involved in N-glycosylation
evolution
isozyme DPY19L1 activity is conserved in mammals and Caenorhabditis elegans, whereas DPY19L3 has acquired another activity, mannosylation of W3 of the WxxWxxWxxC motif in TSRs. DPY19L1 and DPY19L3 act on the first two and the third tryptophan, respectively
evolution
Plasmodium falciparum DPY19 exhibits a different acceptor specificity than the mammalian enzymes
malfunction
identical Q neuroblast migration phenotypes of dpy-19 and mig-21 mutants
malfunction
knockdown of DPY19L3 inhibits the secretion of Rspo1. Lec15.2 cells lack dolichol-phosphate-mannose synthesis activity
malfunction
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no activity in CHO cell mutant cells Lec35.1
malfunction
a DPY-19 mutant shows a dumpy phenotype and a defect in Q neuroblast migration
malfunction
inactivation of DPY19L1 but not DPY19L3 strongly reduces the secretion of UNC5A TSRs and leads to the accumulation of the membrane bound protein in the endoplasmmic reticulum
malfunction
Pf DPY19 gene disruption is not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding
physiological function
Caenorhabditis elegans surface receptors MIG-21 and UNC-5 are acceptor substrates of DPY-19, and C-mannosylation is essential for the secretion of soluble MIG-21
physiological function
DPY19L3-mediated C-mannosylation of Rspo1 at W156 is required for its secretion. R-spondin1 (Rspo1) is a secreted protein that enhances Wnt signaling, which has crucial functions in embryonic development and several cancers. C-mannosylation is a posttranslational modification of the first tryptophan residue in the consensus sequence W-X-X-W/C (in which X represents any amino acid) by an endoplasmic reticulum-localized enzyme. C-mannosylation is important for proteinprotein interactions. Enhancement of canonical Wnt signaling by Rspo1 is regulated by C-mannosylation
physiological function
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the enzyme catalyzes post-translational modification of thrombospondin type-1 repeats in ADAMTS-like and punctin-1 by C-mannosylation of tryptophan
physiological function
apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family
physiological function
apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Plasmodium falciparum Dpy19 is a C-mannosyltransferase
physiological function
apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Toxoplasma gondii Dpy19 is a C-mannosyltransferase
physiological function
enzyme DPY19L3 is a C-mannosyltransferase of R-spondin1 in human cells. DPY19L3 is predicted to be a multipass transmembrane protein that localizes to the endoplasmic reticulum (ER)
physiological function
mannosylation by DPY19L1 but not DPY19L3 is required for transport of UNC5A from the endoplasmic reticulum to the cell surface. The importance of C-mannosylation for protein secretion might reflect a function in protein folding
physiological function
Plasmodium falciparum C-mannosyltransferase (Pf DPY-19) is demonstrated to modify thrombospondin type 1 repeat (TSR) domains in vitro, exhibiting a different acceptor specificity than their mammalian counterparts. The Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development
physiological function
the importance of C-mannosylation for protein secretion might reflect a function in protein folding
physiological function
the importance of C-mannosylation for protein secretion might reflect a function in protein folding
physiological function
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apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Toxoplasma gondii Dpy19 is a C-mannosyltransferase
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physiological function
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apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Toxoplasma gondii Dpy19 is a C-mannosyltransferase
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additional information
among the different types of protein glycosylation, C-mannosylation of tryptophan residues stands out because of the unique linkage formed between sugar and protein. Instead of the typical O- or N-glycosidic linkage, a C-C bond is used for attachment of a single mannose. C-mannose is characteristically found in thrombospondin type 1 repeats and in the WSXWS motif of type I cytokine receptors
additional information
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among the different types of protein glycosylation, C-mannosylation of tryptophan residues stands out because of the unique linkage formed between sugar and protein. Instead of the typical O- or N-glycosidic linkage, a C-C bond is used for attachment of a single mannose. C-mannose is characteristically found in thrombospondin type 1 repeats and in the WSXWS motif of type I cytokine receptors
additional information
the C-terminal luminal region of DPY19L3 is important for the C-mannosyltransferase activity, while N-glycosylations are not
additional information
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the C-terminal luminal region of DPY19L3 is important for the C-mannosyltransferase activity, while N-glycosylations are not
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E206N
site-directed mutatgenesis, the mutation of DPY19L3, resulting in the N-glycosylation consensus sequence (206NFT208), does not induce N-glycosylation, confirming the region is certainly a cytoplasmic region
N118Q
site-directed mutagenesis of the N-glycosylation site
N118Q/N704Q
site-directed mutagenesis of N-glycosylation sites
N319Q
site-directed mutagenesis of a potential N-glycosylation site
N439Q
site-directed mutagenesis of a potential N-glycosylation site
N704Q
site-directed mutagenesis of the N-glycosylation site
additional information
enzyme knockdown by siRNA
additional information
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enzyme knockdown by siRNA
additional information
DPY19L3 has a splice variant, called isoform2, which lacks exons 16-19 and retains part of the intron sequence, resulting in a 542-amino-acid protein. Based on our topological model, isoform2 lacks the C-terminal luminal region of DPY19L3. Topological prediction of isoform2 is almost similar to wild-type DPY19L3, but the last transmembrane (TM) region of wild-type DPY19L3 is changed to re-entrant loop (RL), resulting in the C-terminus of isoform2 is predicted to localize cytoplasm. By redox-sensitive luciferase assay, the C-terminus of isoform2 has high Gaussia luciferase activity, as does this segment of DPY19L3, demonstrating the C-terminus of isoform2 is localized to ER lumen. Furthermore, isoform2 is localized in the ER similar to wild-type DPY19L3
additional information
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DPY19L3 has a splice variant, called isoform2, which lacks exons 16-19 and retains part of the intron sequence, resulting in a 542-amino-acid protein. Based on our topological model, isoform2 lacks the C-terminal luminal region of DPY19L3. Topological prediction of isoform2 is almost similar to wild-type DPY19L3, but the last transmembrane (TM) region of wild-type DPY19L3 is changed to re-entrant loop (RL), resulting in the C-terminus of isoform2 is predicted to localize cytoplasm. By redox-sensitive luciferase assay, the C-terminus of isoform2 has high Gaussia luciferase activity, as does this segment of DPY19L3, demonstrating the C-terminus of isoform2 is localized to ER lumen. Furthermore, isoform2 is localized in the ER similar to wild-type DPY19L3
additional information
CRISPR/Cas9 technology is used to generate a Pf DPY19 null mutant. Pf DPY19 gene disruption is not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding
additional information
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CRISPR/Cas9 technology is used to generate a Pf DPY19 null mutant. Pf DPY19 gene disruption is not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding
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gene dpy-19, recombinant expression in Drosophila melanogaster S2 cells, coexpression with receptor proteins UNC-5 or MIG-21
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells
gene dpy19, recombinant enzyme expression in Drosophila melanogaster S2 cells
gene Dpy19l1, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4. Overexpression of DPY19L1 results in detection of TSR1 peptides with a maximum of two C-mannoses
gene Dpy19l3, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4. The ratio of this C-mannosylated peptide to the nonmannosylated peptide is higher in cells overexpressing DPY19L3 than in wild-type cells
gene Dpy19l4, using CRISPR-Cas9 mutagenesis, Chinese hamster ovary (CHO) single knockout cells are generated as well as a triple knockout lacking DPY19L1, -L3, and -L4, and the respective mouse enzymes are reintroduced in the cells. Transient expression of C-terminal V5-tagged UNC5A in CHO cell single knockouts of DPY19L1, -L3, and -L4
recombinant expression in S2 cells, coexpression with substrate Rspo1
transient recombinant expression of wild-type and mutant enzymes in Drosophila melanogaster S2 cells or HEK-293T cells fused to Gaussia luciferase or tagged with myc or myc-His6 tags, quantitative RT-PCR expression analysis
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells
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Wang, L.W.; Leonhard-Melief, C.; Haltiwanger, R.S.; Apte, S.S.
Post-translational modification of thrombospondin type-1 repeats in ADAMTS-like 1/punctin-1 by C-mannosylation of tryptophan
J. Biol. Chem.
284
30004-30015
2009
Cricetulus griseus
brenda
Niwa, Y.; Suzuki, T.; Dohmae, N.; Simizu, S.
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells
Mol. Biol. Cell
27
744-756
2016
Homo sapiens (Q6ZPD9), Homo sapiens
brenda
Buettner, F.F.; Ashikov, A.; Tiemann, B.; Lehle, L.; Bakker, H.
C. elegans DPY-19 is a C-mannosyltransferase glycosylating thrombospondin repeats
Mol. Cell
50
295-302
2013
Caenorhabditis elegans (P34413), Caenorhabditis elegans
brenda
Niwa, Y.; Nakano, Y.; Suzuki, T.; Yamagishi, M.; Otani, K.; Dohmae, N.; Simizu, S.
Topological analysis of DPY19L3, a human C-mannosyltransferase
FEBS J.
285
1162-1174
2018
Homo sapiens (Q6ZPD9), Homo sapiens
brenda
Hoppe, C.M.; Albuquerque-Wendt, A.; Bandini, G.; Leon, D.R.; Shcherbakova, A.; Buettner, F.F.R.; Izquierdo, L.; Costello, C.E.; Bakker, H.; Routier, F.H.
Apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family
Glycobiology
28
333-343
2018
Toxoplasma gondii (B9QPM1), Toxoplasma gondii, Plasmodium falciparum (C0H4S1), Plasmodium falciparum, Caenorhabditis elegans (P34413), Caenorhabditis elegans, Toxoplasma gondii VEG (B9QPM1), Toxoplasma gondii ATCC 50861 (B9QPM1)
brenda
Lopez-Gutierrez, B.; Cova, M.; Izquierdo, L.
A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development
Parasitology
146
1767-1772
2019
Plasmodium falciparum (C0H4S1), Plasmodium falciparum
brenda
Shcherbakova, A.; Tiemann, B.; Buettner, F.F.; Bakker, H.
Distinct C-mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian DPY19L1 and DPY19L3
Proc. Natl. Acad. Sci. USA
114
2574-2579
2017
Mus musculus (A2AJQ3), Mus musculus (A6X919), Mus musculus (Q71B07), Mus musculus, Caenorhabditis elegans (P34413), Caenorhabditis elegans
brenda