Information on EC 2.4.1.81 - flavone 7-O-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Gunneridae

EC NUMBER
COMMENTARY
2.4.1.81
-
RECOMMENDED NAME
GeneOntology No.
flavone 7-O-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone = UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
apigenin glycosides biosynthesis
-
Flavone and flavonol biosynthesis
-
luteolin glycosides biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:5,7,3',4'-tetrahydroxyflavone 7-O-beta-D-glucosyltransferase
A number of flavones, flavanones and flavonols can function as acceptors. Different from EC 2.4.1.91 (flavonol 3-O-glucosyltransferase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AtGT-2
Q8VZE9
-
glucosyltransferase, uridine diphosphoglucose-apigenin 7-O-
-
-
-
-
glucosyltransferase, uridine diphosphoglucose-luteolin
-
-
-
-
UDP-glucose-apigenin beta-glucosyltransferase
-
-
-
-
UDP-glucosyltransferase
-
-
-
-
UDPglucose-luteolin beta-D-glucosyltransferase
-
-
-
-
uridine diphosphoglucose-apigenin 7-O-glucosyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37332-50-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
TDP-glucose + luteolin
TDP + 7-O-beta-D-glucosylluteolin
show the reaction diagram
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
show the reaction diagram
Q8VZE9
78.9% of the activity with eriodictyol
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
show the reaction diagram
Q8VZE9
64.1% of the activity with eriodictyol
-
-
?
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
show the reaction diagram
-
involved in biosynthesis of flavone glucosides
-
-
?
UDP-glucose + eriodictyol
UDP + 7-O-beta-D-glucosyl-eriodictyol
show the reaction diagram
Q8VZE9
-
-
-
?
UDP-glucose + luteolin
UDP + 7-O-beta-D-glucosyl-luteolin
show the reaction diagram
Q8VZE9
92.1% of the activity with eriodictyol
-
-
?
UDP-glucose + naringenin
UDP + 7-O-beta-D-glucosyl-naringenin
show the reaction diagram
Q8VZE9
71.6% of the activity with eriodictyol
-
-
?
UDPglucose + 3,7,4'-trihydroxyflavone
UDP + 7-O-beta-D-glucosyl-3,7,4'trihydroxyflavone
show the reaction diagram
-
9% of the activity with apigenin
-
-
?
UDPglucose + 5,7-dihydroxyflavanone
UDP + 7-O-beta-D-glucosyl-5,7-dihydroxyflavanone
show the reaction diagram
-
15% of the activity with apigenin
-
-
?
UDPglucose + 7,4'-dihydroxyflavone
UDP + 7-O-beta-D-glucosyl-7,4'-dihydroxyflavone
show the reaction diagram
-
20% of the activity with apigenin
-
-
?
UDPglucose + acacetin
UDP + 7-O-beta-D-glucosylacacetin
show the reaction diagram
-
24% of the activity with apigenin
-
-
?
UDPglucose + apigenin
UDP + 7-O-beta-D-glucosylluteolin
show the reaction diagram
-
-
-
?
UDPglucose + apigenin
UDP + 7-O-beta-D-glucosyl-apigenin
show the reaction diagram
Q8VZE9
29.6% of the activity with eriodictyol
-
-
?
UDPglucose + chrysoeriol
UDP + 7-O-beta-D-glucosylchrysoeriol
show the reaction diagram
-
63% of the activity with apigenin
-
-
?
UDPglucose + datiscetin
UDP + 7-O-beta-D-glucosyldatiscetin
show the reaction diagram
-
9% of the activity with apigenin
-
-
?
UDPglucose + fisetin
UDP + 7-O-beta-D-glucosylfisetin
show the reaction diagram
-
14% of the activity with apigenin
-
-
?
UDPglucose + galangin
UDP + 7-O-beta-D-glucosylgalangin
show the reaction diagram
-
9% of the activity with apigenin
-
-
?
UDPglucose + kaempferid
UDP + 7-O-beta-D-glucosylkaempferid
show the reaction diagram
-
16% of the activity with apigenin
-
-
?
UDPglucose + kaempferol
UDP + 7-O-beta-D-glucosylkaempferol
show the reaction diagram
-
11% of the activity with apigenin
-
-
?
UDPglucose + luteolin
UDP + 7-O-beta-D-glucosylluteolin
show the reaction diagram
-
210% of the activity with apigenin
-
?
UDPglucose + naringenin
UDP + 7-O-beta-D-glucosylnaringenin
show the reaction diagram
-
45% of the activity with apigenin
-
-
?
UDPglucose + quercetin
UDP + 7-O-beta-D-glucosylquercetin
show the reaction diagram
-
16% of the activity with apigenin
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
show the reaction diagram
-
involved in biosynthesis of flavone glucosides
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ethylene monomethylether
-
-
Tris-HCl
-
in 1 M Tris-HCl buffer the reaction rate is 70% of that observed in 0.1 M buffer
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Bovine serum albumin
-
stimulates
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0027
-
apigenin
-
-
0.0018
-
eriodictyol
Q8VZE9
pH 7.0
0.0263
-
kaempferol
Q8VZE9
pH 7.0
0.0015
-
luteolin
-
-
0.01
-
Naringenin
-
-
0.0353
-
quercetin
Q8VZE9
pH 7.0
0.26
-
TDPglucose
-
reaction with apigenin
0.12
-
UDPglucose
-
reaction with apigenin
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
9
-
pH 6.0: about 35% of maximal activity, pH 9.0: about 50% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
extractable activity is drastically increased by illuminating the cells with white light and the maximum activity is reached 24 h after the onset of illumination
Manually annotated by BRENDA team
Q8VZE9
high expression
Manually annotated by BRENDA team
Q8VZE9
high expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q8VZE9
x * 54800, calculated from sequence
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
rapid loss of activity below
7.5
8.5
-
most stable
9.5
-
-
above, rapid loss of activity above
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3°C, t1/2: 1 month in 0.05 M Tris-HCl buffer, pH 7.5, 7 mM 2-mercaptoethanol
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli as a glutathione S-transferase fusion protein
Q8VZE9
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
Q8VZE9
biotransformation of eriodictyol using Escherichi coli expressing AtGT-2