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Information on EC 2.4.1.275 - neolactotriaosylceramide beta-1,4-galactosyltransferase and Organism(s) Homo sapiens

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Homo sapiens
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta4gal-t4, beta-1,4-galt iv, lactotriaosylceramide:galactosyltransferase, udp-galactose:beta-n-acetylglucosamine beta1,4-galactosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-Gal:glucosylceramide beta1->4galactosyltransferase
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-
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beta-1,4-GalT IV
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beta4Gal-T4
lactosylceramide synthase
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UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase
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UDPGal:betaGlcNAc beta1,4Gal-transferase
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uridine diphosphate-galactose:glucosyl ceramide, beta 1-4 galactosyltransferase
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactose:N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1beta4)-beta-D-glucosyl-(1<->1)-ceramide 4-beta-D-galactosyltransferase
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + 1-O-benzyl 6-O-beta-D-GlcpNAC-beta-D-GlypNAc
UDP + 1-O-benzyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-beta-D-GlcpNAc
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 1-O-benzyl beta-D-GlcpNAc
UDP + 1-O-benzyl 4-O-beta-D-Galp-beta-D-GlcpNAc
show the reaction diagram
no activity with alpha-D-GlcNAc-1-benzyl
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-
?
UDP-alpha-D-galactose + 1-O-methyl 3-O-beta-D-GlcpNAc-beta-D-Galp
UDP + 1-O-methyl 4-O-beta-D-Galp-3-O-beta-D-GlcpNAc-beta-D-Galp
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 4-nitrophenyl N-acetyl-1-thio-beta-D-glucosaminide
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosiy-N-acetyl-1-thio-beta-D-glucosaminide
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
UDP-galactose + 1-O-(1-methylumbellifer-7-yl) beta-D-GlcpNAc
UDP + 1-O-(1-methylumbellifer-7-yl) 4-O-beta-D-Galp-beta-D-GlcpNAc
show the reaction diagram
-
-
-
?
UDP-galactose + 1-O-(4-nitrophenyl) beta-D-GlcpNAc
UDP + 1-O-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-GlcpNAc
show the reaction diagram
-
-
-
?
UDP-galactose + 1-O-methyl 6-O-beta-D-GlcpNAC-alpha-D-Manp
UDP + 1-O-methyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-alpha-D-Manp
show the reaction diagram
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-
-
?
UDP-galactose + 1-S-(4-nitrophenyl) beta-D-1-thio-GlcpNAc
UDP + 1-S-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-1-thio-GlcpNAc
show the reaction diagram
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-
-
?
UDP-galactose + beta-D-GlcpNAc-(1->6)-D-GlcpNAc
UDP + beta-D-Galp-(1->4)-[beta-D-GlcpNAc-(1->6)]-D-GlcpNAc
show the reaction diagram
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-
-
?
UDP-galactose + GlcNAc-beta-(1->6)-(GlcNAc-beta-(1->2))-Man-alpha-(1->3)-Man-beta-(1->)O(CH2)8COOCH2-m-nitrophenol
UDP + Gal-alpha-(1->4)-GlcNAc-beta-(1->6)-(GlcNAc-beta-(1->2))-Man-alpha-(1->3)-Man-beta-(1->)O(CH2)8COOCH2-m-nitrophenol
show the reaction diagram
preferential galactosylation of the GlcNAc-beta-(1->6)-Man branch
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-
?
UDP-galactose + N,N',N''-triacetylchitotriose
UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
show the reaction diagram
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-
-
?
UDP-galactose + N,N'-diacetylchitobiose
UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-lactalbumin
the enzyme shows a marked increase in N-acetyllactosamine synthase activity in the presence of alpha-lactalbumin. Two-fold activation is achieved at 0.25 mg/ml and almost 8fold at 20 mg/ml
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.238
4-nitrophenyl N-acetyl-1-thio-beta-D-glucosaminide
pH 7.0, temperature not specified in the publication
0.105
UDP-galactose
pH 7.0, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
beta-1,4-GalTs II, III, IV, V, and VI are involved in the N-linked oligosaccharide biosynthesis cooperatively but not in a redundant manner with beta-1,4-GalT I within cells
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
B4GT1_HUMAN
398
1
43920
Swiss-Prot
Mitochondrion (Reliability: 4)
B4GT3_HUMAN
393
1
43928
Swiss-Prot
Secretory Pathway (Reliability: 3)
B4GT4_HUMAN
344
1
40041
Swiss-Prot
Secretory Pathway (Reliability: 1)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in insect cells
expression in Sf-9 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Guo, S.; Sato, T.; Shirane, K.; Furukawa, K.
Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells
Glycobiology
11
813-820
2001
Homo sapiens (O60513)
Manually annotated by BRENDA team
Schwientek, T.; Almeida, R.M., Levery, S.B.; Holmes, E.H.; Bennett, E.; Clausen, H.
Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis
J. Biol. Chem.
273
29331-29340
1998
Homo sapiens (O60513), Homo sapiens
Manually annotated by BRENDA team