Information on EC 2.4.1.243 - 6G-fructosyltransferase

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The expected taxonomic range for this enzyme is: Liliopsida

EC NUMBER
COMMENTARY
2.4.1.243
-
RECOMMENDED NAME
GeneOntology No.
6G-fructosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n alpha-D-glucopyranoside = [1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >= 0)
show the reaction diagram
m > 0, n ? 0
-
-
-
SYSTEMATIC NAME
IUBMB Comments
1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase
Inulins are polysaccharides consisting of linear or branched D-fructofuranosyl chains attached to the fructosyl residue of sucrose by a beta(2->1) linkage. This enzyme catalyses the transfer of the terminal (21)-linked -D-fructosyl group of an inulin chain onto O-6 position of the glucose residue of another inulin molecule [1]. For example, if 1-kestose [1F-(beta-D-fructofuranosyl)sucrose] is both the donor and recipient in the reaction shown above, i.e., if m = 1 and n = 1, then the products will be sucrose and 6G-di-beta-D-fructofuranosylsucrose. In this notation, the superscripts F and G are used to specify whether the fructose or glucose residue of the sucrose carries the substituent. Alternatively, this may be indicated by the presence and/or absence of primes (see {iupac/2carb/36#362::http://www.chem.qmul.ac.uk/iupac/2carb/36.html#362}). Sucrose cannot be a donor substrate in the reaction (i.e. m cannot be zero) and inulin cannot act as an acceptor. Side reactions catalysed are transfer of a beta-D-fructosyl group between compounds of the structure 1F-(1-beta-D-fructofuranosyl)m-6G-(1-beta-D-fructofuranosyl)n sucrose, where m >= 0 and n = 1 for the donor, and m >= 0 and n >= 0 for the acceptor.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6(G)-fructosyltransferase/2,1-fructan:2,1-fructan 1-fructosyltransferase
P92916
bifunctional enzyme
6G-FFT
-
-
6G-FFT
Agave tequilana azul
-
-
-
6G-FFT
-
-
6G-FFT
P92916
-
6G-FFT
Q5FC15
-
6G-FFT
Q05613
-
6G-FFT
Lolium perenne Bravo
Q8S337
-
-
6G-FFT/1-FFT
Q8S337
-
AoFT1
Q5FC15
-
Atq6G-FFT-1
-
gene name
Atq6G-FFT-1
Agave tequilana azul
-
gene name
-
Atq6G-FFT-2
-
gene name
Atq6G-FFT-2
Agave tequilana azul
-
gene name
-
fructan-fructan 6G-fructosyltransferase
Q8S337
-
fructan: fructan 6G-fructosyltransferase
-
-
fructan: fructan 6G-fructosyltransferase
Agave tequilana azul
-
-
-
fructan:fructan 6G-fructosyltransferase
P92916
-
fructan:fructan 6G-fructosyltransferase
Q5FC15
-
fructan:fructan 6G-fructosyltransferase
Q05613
-
fructan:fructan 6G-fructosyltransferase
Q8S337
-
fructan:fructan 6G-fructosyltransferase
Lolium perenne Bravo
Q8S337
-
-
fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase
Q8S337
-
CAS REGISTRY NUMBER
COMMENTARY
79633-28-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
genes Atq6G-FFT-1 and -2
-
-
Manually annotated by BRENDA team
Agave tequilana azul
genes Atq6G-FFT-1 and -2
-
-
Manually annotated by BRENDA team
bifunctional 6(G)-fructosyltransferase/2,1-fructan:2,1-fructan 1-fructosyltransferase
Swissprot
Manually annotated by BRENDA team
cv. Tenshin, enzyme activity remains stable during 16 weeks of storage at 15C, but decreases thereafter and increases during week 23 and 24 to almost original level
-
-
Manually annotated by BRENDA team
enzyme activity increases progrossively at 10C and at 20C during the first four months of storage and remains stable during the following two months. Acitivity at 10C is slightly higher than at 20C
-
-
Manually annotated by BRENDA team
expression in Cichorium intybus and Nicotiana tabacum
Swissprot
Manually annotated by BRENDA team
expression in Nicotiana tabacum
-
-
Manually annotated by BRENDA team
cultivar HLA-7
Swissprot
Manually annotated by BRENDA team
expression in Pichia pastoris, enzyme has slight fructan:fructan 1-fructosyltransferase activity
Swissprot
Manually annotated by BRENDA team
; perennial ryegrass
SwissProt
Manually annotated by BRENDA team
cultivar Aberystwyth S23
SwissProt
Manually annotated by BRENDA team
cultivars Aurora and Perma
UniProt
Manually annotated by BRENDA team
cv. Bravo
SwissProt
Manually annotated by BRENDA team
Lolium perenne Bravo
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
Q8S337
the enzyme is required for fructan biosynthesis. In the second step, a 6G-FFT produces 6G-kestotriose, using 1-kestotriose as fructosyl donor and sucrose as fructosyl acceptor. In further steps, higher polymeric fructans are synthesized by a 1-FFT activity through beta(2-1)-linked chain elongation or by a 6-FT activity through beta(2-6)-linked chain elongation.The 6G-FFT from Lolium perenne also has 1-FFT activity
metabolism
-
the enzyme is required for fructan biosynthesis
metabolism
Agave tequilana azul
-
the enzyme is required for fructan biosynthesis
-
metabolism
Lolium perenne Bravo
-
the enzyme is required for fructan biosynthesis. In the second step, a 6G-FFT produces 6G-kestotriose, using 1-kestotriose as fructosyl donor and sucrose as fructosyl acceptor. In further steps, higher polymeric fructans are synthesized by a 1-FFT activity through beta(2-1)-linked chain elongation or by a 6-FT activity through beta(2-6)-linked chain elongation.The 6G-FFT from Lolium perenne also has 1-FFT activity
-
additional information
-
the enzyme activity varies slightly during storage of fresh spears in the dark at 10C for 14 days, while the sugar content is rapidly lost, overview
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
35
Q8S337
-
30
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
40
Q8S337
20C: about 80% of maximal activity, 40C: about 80% of maximal activity
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.11
-
-
Atq6G-FFT-2, sequence calculation
5.4
-
Q5FC15
calculated
5.57
-
-
Atq6G-FFT-1, sequence calculation
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
Atq6G-FFT-1 and Atq6G-FFT-2 contains each seven glycosylation sites
glycoprotein
Agave tequilana azul
-
Atq6G-FFT-1 and Atq6G-FFT-2 contains each seven glycosylation sites
-
glycoprotein
Q5FC15
sequence contains six potential N-glycosilation sites
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
several attempts to purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeasts expression medium
Q8S337