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Information on EC 2.4.1.206 - lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase and Organism(s) Homo sapiens
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2.4.1.206 lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferaseSpecify your search results
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
glcnac transferase, beta3gn-t2, beta3gn-t5, lc3 synthase, lactosylceramide n-acetylglucosaminyltransferase, beta3gnt5, la2 synthase, lc3 synthase/bgn-t5, beta1-3-n-acetylglucosaminyltransferase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-lactosylceramide beta-
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-
-
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beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase
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beta1-3-N-acetylglucosaminyltransferase
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LA2 synthase
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-
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lactosylceramide beta-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-lactosylceramide beta-acetylglucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3-beta-N-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
83682-80-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1-4)-beta-D-glucosyl-(1-1)-ceramide
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
?
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
additional information
?
-
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beta3Gn-T2 associates with beta3Gn-T8 in vivo
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
best substrate
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
acceptor substrate specificity, overview
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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i.e. lactosylceramide
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
i.e. lactosylceramide
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
i.e. lactosylceramide
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
ganglioside metabolism and pattern
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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biosynthetic pathway of lactoseries and ganglioseries
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-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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may play a key role in regulating the level of various types of lactoseries tumor-associated antigens with the lacto type 1 or 2 chain
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
additional information
?
-
-
beta3Gn-T2 associates with beta3Gn-T8 in vivo
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
ganglioside metabolism and pattern
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
biosynthetic pathway of lactoseries and ganglioseries
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
may play a key role in regulating the level of various types of lactoseries tumor-associated antigens with the lacto type 1 or 2 chain
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
down-regulation of enzyme expression
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.42
Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
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betaGn3-T2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00123
recombinant enzyme, substrate lD-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
0.00174
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.0021
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.00254
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
additional information
additional information
-
activity in different cell lines of normal colonic mucosa and colonic adenocarcinoma
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
Daoy and D341 Med, cultured both in vitro and as xenografts in nude mice
additional information
additional information
transcript content and activity in diverse tumour cell lines, overview, nearly undetectable content in leukemic cell lines
additional information
-
transcript content and activity in diverse tumour cell lines, overview, nearly undetectable content in leukemic cell lines
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). B3GN5_HUMAN
378
1
44053
Swiss-Prot
Secretory Pathway (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
expression in Escherichia coli, overexpression of beta3GnT5 in MNK-45 cells and AGS cells, transfection with lipofectamine
-
soluble expression as FLAG-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
adhesion of Helicobacter pylori to epithelial cells is mediated by bacterial adhesins that bind carbohydrate ligands expressed by the epithelial gastric cells, bea3GnT5 transferase induction is specific to infection with highly pathogenic strains
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gottfries, J.; Percy, A.K.; Mansson, J.E.; Fredman, P.; Wikstrand, C.J.; Friedman, H.S.; Bigner, D.D.; Svennerholm, L.
Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas
Biochim. Biophys. Acta
1081
253-261
1991
Homo sapiens
Holmes, E.H.; Hakomori, S.i.; Ostrander, G.K.
Synthesis of type 1 and 2 lacto series glycolipid antigens in human colonic adenocarcinoma and derived cell lines is due to activation of a normally unexpressed beta 1-3N-acetylglucosaminyltransferase
J. Biol. Chem.
262
15649-15658
1987
Homo sapiens
Togayachi, A.; Akashima, T.; Ookubo, R.; Kudo, T.; Nishihara, S.; Iwasaki, H.; Natsume, A.; Mio, H.; Inokuchi, J.i.; Irimura, T.; Sasaki, K.; Narimatsu, H.
Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T5), an essential enzyme for the expression of HNK-1 and lewis X epitopes on glycolipids
J. Biol. Chem.
276
22032-22040
2001
Homo sapiens (Q9BYG0), Homo sapiens, Rattus norvegicus, Rattus norvegicus (Q99NB2)
Henion, T.R.; Zhou, D.; Wolfer, D.P.; Jungalwala, F.B.; Hennet, T.
Cloning of a mouse beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis
J. Biol. Chem.
276
30261-30269
2001
Homo sapiens (Q9BYG0), Mus musculus (Q8BGY6), Mus musculus, no activity in Spodoptera frugiperda
Marcos, N.T.; Magalhaes, A.; Ferreira, B.; Oliveira, M.J.; Carvalho, A.S.; Mendes, N.; Gilmartin, T.; Head, S.R.; Figueiredo, C.; David, L.; Santos-Silva, F.; Reis, C.A.
Helicobacter pylori induces beta 3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl-Lewis x
J. Clin. Invest.
118
2325-2336
2008
Homo sapiens
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