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Information on EC 2.4.1.201 - alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC2.4.1.201

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2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.201 alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Requires a high concentration of Mn2+ for maximal activity. The enzyme, characterized from hen oviduct membranes, participates in the processing of N-glycans in the Golgi apparatus. It transfers GlcNAc in beta1-4 linkage to a D-mannose residue that already has GlcNAc residues attached at positions 2 and 6 by beta linkages. No homologous enzyme appears to exist in mammals.

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Word Map

2.4.1.201
5-phosphate
1-deoxy-d-xylulose
fosmidomycin
isoprenoids
valine
falciparum
methylerythritol
reductoisomerase
isoleucine
n-glycans
1-deoxyxylulose
mtdna

The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms

Synonyms

gnt vi, mgat6, n-acetylglucosaminyltransferase vi, show all synonyms

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acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta-1,4-, VI

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acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-glycopeptide beta-1-4-, VI

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GCNT6

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GnT VI

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GnT-VI

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mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase

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Mgat6

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N-acetylglucosaminyltransferase VI

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N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase VI

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UDP-GlcNAc: GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man] beta1-4N-acetylglucosaminyltransferase VI

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uridine diphosphoacetylglucosamine-glycopeptide beta-1-4-acetylglucosaminyltransferase VI

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Go to Reaction Type Search

hexosyl group transfer

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Go to Pathway Search

KEGG

N-Glycan biosynthesis

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Go to Systematic Name Search

UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->2)]-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)

Requires a high concentration of Mn2+ for maximal activity. The enzyme, characterized from hen oviduct membranes, participates in the processing of N-glycans in the Golgi apparatus. It transfers GlcNAc in beta1-4 linkage to a D-mannose residue that already has GlcNAc residues attached at positions 2 and 6 by beta linkages. No homologous enzyme appears to exist in mammals.

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119699-68-2

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Go to Substrate/Product Search

UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R

UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R

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evaluation of a highly sensitive method for determination of the activity of GnT VI using a fl uorescently labeled tetraantennary oligosaccharide as an acceptor substrate. GnT VI stringently requires beta1,6-N-acetylglucosaminylation on the Manalpha1-6 arm (GnT V product, EC 2.4.1.155) as an acceptor substrate. Agalacto-triantennary oligosaccharide (GnT V product) can also be used as an alternate substrate

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UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R

UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R

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Go to Metals and Ions Search

Co2+

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activates

Mg2+

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activates

Mn2+

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required, best divalent cation

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divalent cation-dependent enzyme activity, maximal activity with Mn2+. Co2+ and Mg 2+ can partially substitute for Mn2+

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Go to pH Optimum Search

8

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assay at

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37

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assay at

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Go to General Information Search

metabolism

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physiological function

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the enzyme contributes to the surface expression of CD133 through the processing of multiantennary N -linked glycans on CD133

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additional information

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GlcNAc and tet-induced Mgat6 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions. The Mgat5, EC 2.4.1.155, product is the preferred acceptor for Mgat6, which adds a GlcNAcbeta1,4 branch that is not present in mammals, Mgat6 enhances functionality of N-glycan branching in mammalian cells

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gene MGAT6, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat6 HEK293 cells produce increased oligosaccharide levels also observed in HeLa cells, predicted to be the 2,2,4,6 tetra-, 4,2,2,4 tetra- and 4,2,2,4,6 penta-N-glycans, while native 4,2,2,6 tetra are reduced, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview

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Abdel Rahman, A.; Ryczko, M.; Nakano, M.; Pawling, J.; Rodrigues, T.; Johswich, A.; Taniguchi, N.; Dennis, J.

Golgi N-glycan branching N-acetylglucosaminyltransferases I, V and VI promote nutrient uptake and metabolism

Glycobiology

25

225-240

2015

Homo sapiens

Manually annotated by BRENDA team

Taguchi, T.; Taniguchi, N.

A method for determination of UDP-GlcNAc: GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man] beta1-4N-acetylglucosaminyltransferase VI activity

Methods Mol. Biol.

1022

299-305

2013

Homo sapiens

Manually annotated by BRENDA team

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Release 2023.1 (January 2023)