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Information on EC 2.4.1.149 - N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase and Organism(s) Homo sapiens

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EC Tree
IUBMB Comments
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
b3gnt3, b3gnt1, beta3gnt7, beta3gnt1, beta3gn-t7, beta-1,3-n-acetylglucosaminyltransferase-3, beta1,3-n-acetylglucosaminyltransferase-7, beta1-3 n-acetylglucosaminyltransferase-1, poly-n-acetyllactosamine extension enzyme, udp-glcnac:betagal beta-1,3-n-acetylglucosaminyltransferase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-
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B3gnt1
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beta-1,3-N-acetylglucosaminyltransferase 1
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beta-1,3-N-acetylglucosaminyltransferase-3
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beta1,3-N-acetylglucosaminyltransferase-7
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beta3-N-acetylglucosaminyltransferase 1
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beta3Gn-T7
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beta3GnT1
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Galbeta1-->4GlcNAc-R beta1-->3 N-acetylglucosaminyltransferase
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GnT-I
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i-GlcNAc transferase
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i-GlcNAcT
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-
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IGnT
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N-acetyllactosamine beta(1-3)N-acetylglucosaminyltransferase
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poly-N-acetyllactosamine extension enzyme
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UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
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UDP-GlcNAc:Galbeta1-->4GlcNAcbeta-Rbeta1-->3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:Galbeta1-4Glc(NAc) beta-1,3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:GalR, beta-D-3-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-acetylglucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R 3-beta N-acetylglucosaminyltransferase (configuration-inverting)
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
CAS REGISTRY NUMBER
COMMENTARY hide
85638-39-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-6)(Galbeta(1-4)GlcNAcbeta(1-2))Manalpha(1-6)Manbeta-octyl
?
show the reaction diagram
-
regioselectivity of enzyme, favored site of GlcNAc addition is the lower beta1,2-branch over the beta1,6-branch by a 3:1 ratio resulting in a mixture of heptasaccharides
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta-p-nitrophenol
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-p-nitrophenol
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
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i.e. L2L4, 72% activity compared to L2L2
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
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i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3Galbeta1,4(SO3-,6)-GlcNAc
show the reaction diagram
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i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate
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-
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
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mixture of the pentasaccharide product, 27 mol%, and the unreacted tetrasaccharide, 73 mol%
?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta(1-3)Galbeta1-OMe
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OMe
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OMe
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + GalNAcbeta(1-4)GlcNAcbeta1-OR
UDP + GlcNAcbeta(1-3)GalNAcbeta(1-4)GlcNAcbeta1-OR
show the reaction diagram
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i.e. N,N’-diacetyllactosediamine-OR
only 12.5% of the substrate is converted into the product, which is unusually resistant towards jackbean beta-N-acetylhexosaminidase
?
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
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polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
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?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
poly-N-acetyllactosamine is biosynthesized by the alternating addition of N-acetyl-D-glucosamine by UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase and Gal by UDP-Gal:betaGlcNAc beta-1,4-galactosyltransferase
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?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
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?
UDP-N-acetylglucosamine + lactoneotetraosylceramide
?
show the reaction diagram
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lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length
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?
UDP-N-acetylglucosamine + lactonorhexaosylceramide
?
show the reaction diagram
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lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length
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?
UDP-N-acetylglucosamine + lactose
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
UDP-N-acetylglucosamine + lactosylceramide
?
show the reaction diagram
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lactosylceramide from human erythrocytes
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?
UDP-N-acetylglucosamine + lactotetraosylceramide
?
show the reaction diagram
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lactotetraosylceramide from human meconium, poor substrate
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?
UDP-N-acetylglucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-glycan
?
show the reaction diagram
-
polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans
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?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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about one third of the activity compared to Mn2+, recombinant enzyme
Mg2+
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about one third of the activity compared to Mn2+, recombinant enzyme
additional information
-
not activated by Mg2+ or Ca2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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omitting ATP from reaction mixture decreases activity by 87%
CHAPSO
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activates solubilized enzyme
deoxycholate
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activates solubilized enzyme
G-3634-A
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activates solubilized enzyme
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Triton CF-54
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activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3%
Triton X-100
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activates solubilized enzyme
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.36
Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
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recombinant enzyme, pH 7.2, 37°C
0.19
lactoneotetraosylceramide
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0.19
lactonorhexaosylceramide
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29.8
lactose
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7
N-acetyllactosamine
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0.133 - 0.17
UDP-N-acetyl-D-glucosamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 7.5
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broad
6.5 - 7
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6.8 - 7.8
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broad
7 - 7.2
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highest activity, with Hepes buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highly expressed in fetal and adult brain
Manually annotated by BRENDA team
highly expressed in fetal kidney
Manually annotated by BRENDA team
NSCLC, quantitative real-time PCR enzyme expression analysis
Manually annotated by BRENDA team
colonic carcinoma cell line
Manually annotated by BRENDA team
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colonic adenocarcinoma cell line
Manually annotated by BRENDA team
human melanoma cell line
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
B3GN2_HUMAN
397
1
46022
Swiss-Prot
Secretory Pathway (Reliability: 3)
B3GN3_HUMAN
372
1
42534
Swiss-Prot
Secretory Pathway (Reliability: 1)
B3GN4_HUMAN
378
0
42310
Swiss-Prot
other Location (Reliability: 5)
B4GA1_HUMAN
415
0
47119
Swiss-Prot
-
B3GN7_HUMAN
401
0
45987
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47125
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
2 potential N-glycosylation sites
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells
expression in COS-7 cells, localization in membranes
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gene B3GNT3, real-time PCR enzyme expression analysis
human ortholog to mouse enzyme beta3GnT7 has the chromosomal locus 2q37.1
plasmid cDNA encoding the catalytic domain of i-GlcNAcT, amino acids 53-415, is expressed in COS-1 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
forced expression of beta3GnT1 in aggressive cancer cells restores the laminin-binding glycans and decreased tumor formation
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the decrease of laminin-binding glycans and consequent increased cell migration are associated with the decreased expression of beta3-N-acetylglucosaminyltransferase-1
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
high expression of B3GNT3 is associated with unfavourable disease-free survival and overall survival in NSCLC patients, suggesting that B3GNT3 might be a potential prognostic biomarker for non-small cell lung cancer (NSCLC). B3GNT3 expression is an independent prognostic factor
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zielenski, J.; Koscielak, J.
The occurrence of two novel N-acetylglucosaminyltransferase activities in human serum
FEBS Lett.
158
164-168
1983
Homo sapiens
Manually annotated by BRENDA team
Hosomi, O.; Takeya, A.; Kogure, T.
Human serum contains N-acetyllactosamine: beta 1-3 N-acetylglucosaminyltransferase activity
J. Biochem.
95
1655-1659
1984
Homo sapiens
Manually annotated by BRENDA team
Holmes, E.H.
Characterization of a beta 1-->3-N-acetylglucosaminyltransferase associated with synthesis of type 1 and type 2 lacto-series tumor-associated antigens from the human colonic adenocarcinoma cell line SW403
Arch. Biochem. Biophys.
260
461-468
1988
Homo sapiens
Manually annotated by BRENDA team
McAuliffe, J.C.; Ujita, M.; Fukuda, M.; Hindsgaul, O.
Synthesis of selectively radiolabeled hexasaccharides for the determination of enzymatic regioselectivity
Glycoconjugate J.
16
767-772
1999
Homo sapiens
Manually annotated by BRENDA team
Kataoka, K.; Huh, N.h.
A novel beta1,3-N-acetylglucosaminyltransferase involved in invasion of cancer cells as assayed in vitro
Biochem. Biophys. Res. Commun.
294
843-848
2002
Homo sapiens (Q8NFL0), Homo sapiens, Mus musculus (Q8K0J2), Mus musculus, Mus musculus beta3GnT7 (Q8K0J2)
Manually annotated by BRENDA team
Sasaki, K.; Kurata-Miura, K.; Ujita, M.; Angata, K.; Nakagawa, S.; Sekine, S.; Nishi, T.; Fukuda, M.
Expression cloning of cDNA encoding a human beta-1,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis
Proc. Natl. Acad. Sci. USA
94
14294-14299
1997
Homo sapiens (O43505)
Manually annotated by BRENDA team
Salo, H.; Aitio, O.; Ilves, K.; Bencomo, E.; Toivonen, S.; Penttil, L.; Niemel, R.; Salminen, H.; Grabenhorst, E.; Renkonen, R.; Renkonen, O.
Several polylactosamine-modifying glycosyltransferases also use internal GalNAcbeta1-4GlcNAc units of synthetic saccharides as acceptors
Glycobiology
12
217-228
2002
Homo sapiens
Manually annotated by BRENDA team
Seko, A.; Yamashita, K.
beta1,3-N-Acetylglucosaminyltransferase-7 (beta3Gn-T7) acts efficiently on keratan sulfate-related glycans
FEBS Lett.
556
216-220
2004
Homo sapiens
Manually annotated by BRENDA team
Lee, P.L.; Kohler, J.J.; Pfeffer, S.R.
Association of beta-1,3-N-acetylglucosaminyltransferase 1 and beta-1,4-galactosyltransferase 1, trans-Golgi enzymes involved in coupled poly-N-acetyllactosamine synthesis
Glycobiology
19
655-664
2009
Homo sapiens
Manually annotated by BRENDA team
Bao, X.; Kobayashi, M.; Hatakeyama, S.; Angata, K.; Gullberg, D.; Nakayama, J.; Fukuda, M.; Fukuda, M.
Tumor suppressor function of laminin-binding alpha-dystroglycan requires a distinct beta3-N-acetylglucosaminyltransferase
Proc. Natl. Acad. Sci. USA
106
12109-12114
2009
Homo sapiens
Manually annotated by BRENDA team
Gao, L.; Zhang, H.; Zhang, B.; Zhu, J.; Chen, C.; Liu, W.
B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer
J. Clin. Pathol.
71
642-647
2018
Homo sapiens (Q9Y2A9), Homo sapiens
Manually annotated by BRENDA team