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Information on EC 2.4.1.145 - alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase and Organism(s) Mus musculus

for references in articles please use BRENDA:EC2.4.1.145
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EC Tree
IUBMB Comments
Requires Mn2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. By adding a glucosaminyl residue to biantennary N-linked glycans, it enables the synthesis of tri- and tetra-antennary complexes.
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This record set is specific for:
Mus musculus
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
gnt-iv, gnt-iva, mgat4a, gnt-ivb, n-acetylglucosaminyltransferase iv, gnt iv, mgat4b, n-acetylglucosaminyltransferase-iv, n-acetylglucosaminyltransferase iva, glcnactase-iv, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase
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beta-acetylglucosaminyltransferase IV
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GnT-IVa
GnT-IVb
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isozyme
Mgat4a
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N-acetylglucosaminyltransferase IV
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N-acetylglucosaminyltransferase-IVa
N-acetylglucosaminyltransferase-IVb
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isozyme
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IV
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uridine diphosphoacetylglucosamine-glycopeptide beta4-acetylglucosaminyltransferase IV
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Requires Mn2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. By adding a glucosaminyl residue to biantennary N-linked glycans, it enables the synthesis of tri- and tetra-antennary complexes.
CAS REGISTRY NUMBER
COMMENTARY hide
86498-16-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
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show the reaction diagram
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isozyme GnT-IVb has about 30% activity of isozyme GnT-IVa
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
additional information
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in wild type mice, GnT-IVa expression is restricted to gastrointestinal tissues, whereas GnT-IVb is broadly expressed among organs
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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isozyme GnT-IVb deficiency shows mild phenotypic alterations in hematopoietic cell populations and hemostasis, GnT-IVa/-IVb double deficiency completely abolishes GnT-IV activity that results in the disappearance of the GlcNAcbeta1-4 branch on the Manalpha1-3 arm
physiological function
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the enzyme acts in migration and metastasis of mouse hepatocarcinoma cells through altering the glycosylation of CD147
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MGT4A_MOUSE
535
1
61480
Swiss-Prot
Secretory Pathway (Reliability: 2)
MGT4B_MOUSE
548
1
63302
Swiss-Prot
Secretory Pathway (Reliability: 1)
MGT4C_MOUSE
478
1
56250
Swiss-Prot
Mitochondrion (Reliability: 5)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takamatsu, S.; Antonopoulos, A.; Ohtsubo, K.; Ditto, D.; Chiba, Y.; Le, D.T.; Morris, H.R.; Haslam, S.M.; Dell, A.; Marth, J.D.; Taniguchi, N.
Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice
Glycobiology
20
485-497
2010
Mus musculus
Manually annotated by BRENDA team
Fan, J.; Wang, S.; Yu, S.; He, J.; Zheng, W.; Zhang, J.
N-acetylglucosaminyltransferase IVa regulates metastatic potential of mouse hepatocarcinoma cells through glycosylation of CD147
Glycoconj. J.
29
323-334
2012
Mus musculus
Manually annotated by BRENDA team