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4-nitrophenyl beta-D-xylopyranoside + UDP-alpha-D-galactose
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
-
?
UDP-alpha-D-galactose + (1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
UDP + ?
-
-
-
?
UDP-alpha-D-galactose + (1S,2R,3S,4S)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
UDP + ?
-
-
-
?
UDP-alpha-D-galactose + 2-naphthyl 1,5-dithio-beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-1,5-dithio-beta-D-xylopyranoside
-
-
-
?
UDP-alpha-D-galactose + 2-naphthyl 5-thio-beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranoside
-
-
-
?
UDP-alpha-D-galactose + 2-naphthyl beta-D-xylopyranoside
UDP + 4-beta-D-galactosyl-2-naphthyl-beta-D-xylopyranoside
-
-
-
?
UDP-alpha-D-galactose + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl beta-D-carbaxylopyranoside
UDP + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-beta-D-carbaxylopyranoside
a 5a-carba-beta-xylopyranoside
-
-
?
UDP-alpha-D-galactose + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl beta-L-carbaxylopyranoside
UDP + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-beta-L-carbaxylopyranoside
a 5a-carba-beta-xylopyranoside
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl alpha-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
UDP + ?
the strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay. Molecular modeling of the enzyme's active site in the presence of UDP-alpha-D-galactose and 4-methylumbelliferyl-beta-D-xylopyranoside using the crystal structure of Drosophila melanogaster enzyme dbeta4GalT7, PDB code 4M4K, overview
-
-
?
UDP-alpha-D-galactose + beciparcil
UDP + 4-[(4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranosyl)thio]benzonitrile
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-alpha-D-galactose + iliparcil
UDP + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranoside
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-alpha-D-galactose + naphthalen-2-yl 1-thio-beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-1-thio-beta-D-xylopyranoside
-
-
-
?
UDP-alpha-D-galactose + naphthalen-2-yl beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
?
UDP-alpha-D-galactose + naroparcil
UDP + 4-[4-[(4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranosyl)thio]benzoyl]benzonitrile
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein]
UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
-
-
-
?
UDP-alpha-D-galactose + odiparcil
UDP + 4-methyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranoside
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-galactose + 2-(6-hydroxynaphthyl) beta-D-xylopyranoside
?
-
-
-
-
?
UDP-galactose + 3-acetamido-propyl beta-D-xylopyranoside
?
-
-
-
-
?
UDP-galactose + 3-benzamido-propyl beta-D-xylopyranoside
?
-
-
-
-
?
UDP-galactose + 3-hexanamido-propyl beta-D-xylopyranoside
?
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galacto-pyranosyl-beta-D-xylopyranoside
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
?
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranoyl-beta-D-xylopyranoside
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
-
-
-
?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
UDP + 4-nitrophenyl 4-O-beta-D-galacto-pyranosyl-beta-D-xylopyranoside
-
-
-
-
?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
UDP-galactose + N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
?
-
-
-
-
?
UDP-galactose + N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
?
-
-
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
UDP-galactose + O-gamma-2,3,4-tri-O-acetyl-(beta-D-xylopyranosyl)-N-Cbz-L-serine methyl ester
?
-
-
-
-
?
UDP-galactose + O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
?
-
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-D-xyloside
UDP + Galbeta(1-4)Xylbeta-p-nitrophenyl
-
-
-
-
?
UDP-glucose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
-
-
-
?
UDP-glucuronic acid + 4-methylumbelliferyl-beta-D-xylopyranoside
?
-
-
-
?
UDP-N-acetyl glucosamine + 4-methylumbelliferyl-beta-D-xylopyranoside
?
-
-
-
?
UDP-xylose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
-
-
-
?
UDPgalactose + 4-methylumbelliferyl-beta-D-xyloside
UDP + beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(4-methylumbelliferone)
UDPgalactose + p-nitrophenyl beta-D-xylopyranoside
UDP + beta-D-galactopyranosyl-1,4-xylopyranosyl-1-O-nitrophenol
-
-
-
-
?
additional information
?
-
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
-
?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
-
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
the enzyme is unable to catalyze the transfer of galactose from UDP-galactose onto xylose when this residue is phosphorylated at the C-2 position
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
in a progeroid patient carrying two point mutations in beta4galactosyltransferase I (ß4GalT-7) only 50% of the decorin core protein molecules are substituted with glycosaminoglycan chains. Tthe ß4GalT-7 mutations directly affect the molecular phenotype of decorin observed in a patient with the progeroid form of Ehlers-Danlos syndrome, which may be a major mechanistic cause for the skin and wound healing defects observed in this patient
-
-
?
UDPgalactose + 4-methylumbelliferyl-beta-D-xyloside
UDP + beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(4-methylumbelliferone)
-
-
-
-
?
UDPgalactose + 4-methylumbelliferyl-beta-D-xyloside
UDP + beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(4-methylumbelliferone)
-
assay method for measuring the galactosyltransferase I activity with D-galactal as inhibitor for endogenous beta-galactosidase
-
-
?
additional information
?
-
beta-1,4-galactosyltransferase is responsible for the biosynthesis of Gal-beta - 4GlcNAc units in N-glycans and core 2 O-glycans of glycoproteins, including IgG, gp120 and serum alpha-fetoprotein
-
-
?
additional information
?
-
enzyme is able to transfer glucose, xylose and, to a lesser extent glucuronic acid and N-acetylglucosamine, to acceptor sugars, whereas UDP-mannose and UDP-N-acetyl galactosamine are not substrates
-
-
?
additional information
?
-
-
enzyme is able to transfer glucose, xylose and, to a lesser extent glucuronic acid and N-acetylglucosamine, to acceptor sugars, whereas UDP-mannose and UDP-N-acetyl galactosamine are not substrates
-
-
?
additional information
?
-
some beta-D-xylosides, such as 2-naphthyl beta-D-xylopyranoside, can induce glycosaminoglycan synthesis by serving as acceptor substrates for beta4GalT7 and by that also compete with the glycosaminoglycan synthesis on core proteins
-
-
?
additional information
?
-
donor and acceptor substrate binding, strutcure analysis, overview
-
-
?
additional information
?
-
structure-activity relationships for beta4GalT7 and xylosides with modifications of the aromatic aglycon, using enzymatic assays, cell studies, and molecular docking simulations, show that the aglycons reside on the outside of the active site of the enzyme and that quite bulky aglycons are accepted. Increased galactosylation with increased linker length is observed, substrate specificity with glycosides, detailed overview. The galactosylation is influenced by the identity and position of substituents in the aromatic framework, and generally, only xylosides with beta-glycosidic linkages function as good substrates for the enzyme. The galactosylation ability of a xyloside is increased by replacing the anomeric oxygen with sulfur, but decreased by replacing it with carbon. Enzyme reaction kinetics of galactosylation are dependent on subtle differences in orientation of the xylose moiety
-
-
?
additional information
?
-
the enzyme catalyzes the transfer of galactose from uridine diphosphogalactose to terminal N-acetylglucosamine, forming the Galbeta1-4GlcNAc structure
-
-
?
additional information
?
-
synthesis of xyloside analogues with substitution of the endocyclic oxygen atom by sulfur or carbon and investigation of them as substrates for beta-1,4-galactosyltransferase 7 (beta4GalT7), overview. The analogues with an endocyclic sulfur atom prove to be excellent substrates for b4GalT7, and are galactosylated approx. fifteen times more efficiently than the corresponding xyloside. The 5a-carba-beta-xylopyranoside in the D-configuration proves to be a good substrate for beta4GalT7, whereas the enantiomer in the L-configuration shows no activity. X-ray crystallography, NMR spectroscopy, and molecular modeling provide a rationale for the pronounced activity of the sulfur analogues. Favorable Pi-Pi interactions between the 2-naphthyl moiety and a tyrosine side chain of the enzyme are observed for the thio analogues (1S,2R,3S,4S)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol, 4-ethyl-6-([(1R,2R,3S,4R)-2,3,4-trihydroxycyclohexyl]oxy)-2H-1-benzopyran-2-one, and 4-ethyl-6-([(1S,2S,3R,4S)-2,3,4-trihydroxycyclohexyl]oxy)-2H-1-benzopyran-2-one. The carbaxyloside of iliparcil are synthesized as well as its L-xylo-enantiomers 4-ethyl-6-([(1R,2R,3S,4R)-2,3,4-trihydroxycyclohexyl]oxy)-2H-1-benzopyran-2-one and 4-ethyl-6-([(1S,2S,3R,4S)-2,3,4-trihydroxycyclohexyl]oxy)-2H-1-benzopyran-2-one. The L-xylo-derivative 4-ethyl-6-([(1S,2S,3R,4S)-2,3,4-trihydroxycyclohexyl]oxy)-2H-1-benzopyran-2-one shows a much higher antithrombotic activity compared to the D-xylo-enantiomer 4-ethyl-6-([(1R,2R,3S,4R)-2,3,4-trihydroxycyclohexyl]oxy)-2H-1-benzopyran-2-one and only the compound with L-xylo conformation functions as substrate for beta4GalT7 when assayed with enzyme extracts from chicken embryo. This is in stark contrast to other investigations where L-enantiomers of xylosides rarely act as substrates for beta4GalT7. Quantum mechanical calculations and docking simulations, overview
-
-
-
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Adenocarcinoma
Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells.
Arthritis, Rheumatoid
beta1,4-Galactosyltransferase-I contributes to the inflammatory processes in synovial tissue of patients with rheumatoid arthritis.
Astrocytoma
Over-expression of beta-1,4-galactosyltransferase I, II, and V in human astrocytoma.
Astrocytoma
Over-expression of beta-1,4-galactosyltransferase V increases the growth of astrocytoma cell line.
Breast Neoplasms
Arachidonic acid induces an increase of ?-1,4-galactosyltransferase I expression in MDA-MB-231 breast cancer cells.
Carcinoma, Hepatocellular
Identification of beta-1,4-galactosyltransferase I as a target gene of HBx-induced cell cycle progression of hepatoma cell.
Cataract
Corneal clouding, cataract, and colobomas with a novel missense mutation in B4GALT7-a review of eye anomalies in the linkeropathy syndromes.
Chlamydia Infections
Attachment of Chlamydia trachomatis L2 to host cells requires sulfation.
Chondrosarcoma
Kinetics of intracellular processing of chondroitin sulfate proteoglycan core protein and other matrix components.
Coloboma
Corneal clouding, cataract, and colobomas with a novel missense mutation in B4GALT7-a review of eye anomalies in the linkeropathy syndromes.
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId.
Dwarfism
Current insights into the molecular genetic basis of dwarfism in livestock.
Dwarfism
Dwarfism with joint laxity in Friesian horses is associated with a splice site mutation in B4GALT7.
Ehlers-Danlos Syndrome
Biochemical and thermodynamic characterization of mutated beta1,4-galactosyltransferases 7 involved in the progeroid form of the Ehlers-Danlos syndrome.
Ehlers-Danlos Syndrome
Broadening the phenotypic spectrum of Beta3GalT6-associated phenotypes.
Ehlers-Danlos Syndrome
Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (ss4GalT-7) deficient form of Ehlers-Danlos syndrome.
Ehlers-Danlos Syndrome
Cloning and expression of a proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I. A seventh member of the human beta4-galactosyltransferase gene family.
Ehlers-Danlos Syndrome
Defective glycosaminoglycan substitution of decorin in a patient with progeroid syndrome is a direct consequence of two point mutations in the galactosyltransferase I (beta4GalT-7) gene.
Ehlers-Danlos Syndrome
Defective glycosylation of decorin and biglycan, altered collagen structure, and abnormal phenotype of the skin fibroblasts of an Ehlers-Danlos syndrome patient carrying the novel Arg270Cys substitution in galactosyltransferase I (beta4GalT-7).
Ehlers-Danlos Syndrome
Dwarfism with joint laxity in Friesian horses is associated with a splice site mutation in B4GALT7.
Ehlers-Danlos Syndrome
Expanding the clinical spectrum of B4GALT7 deficiency: homozygous p.R270C mutation with founder effect causes Larsen of Reunion Island syndrome.
Ehlers-Danlos Syndrome
Further Defining the Phenotypic Spectrum of B3GAT3 Mutations and Literature Review on Linkeropathy Syndromes.
Ehlers-Danlos Syndrome
Further defining the phenotypic spectrum of B4GALT7 mutations.
Ehlers-Danlos Syndrome
Galactosyltransferase I is a gene responsible for progeroid variant of Ehlers-Danlos syndrome: molecular cloning and identification of mutations.
Ehlers-Danlos Syndrome
Molecular basis for the progeroid variant of Ehlers-Danlos syndrome. Identification and characterization of two mutations in galactosyltransferase I gene.
Ehlers-Danlos Syndrome
Phenotype and response to growth hormone therapy in siblings with B4GALT7 deficiency.
Ehlers-Danlos Syndrome
Redefining the progeroid form of ehlers-danlos syndrome: Report of the fourth patient with B4GALT7 deficiency and review of the literature.
Ehlers-Danlos Syndrome
Report of two siblings with spondylodysplastic Ehlers-Danlos syndrome and B4GALT7 deficiency.
Glaucoma
Corneal clouding, cataract, and colobomas with a novel missense mutation in B4GALT7-a review of eye anomalies in the linkeropathy syndromes.
Glioblastoma
Over-expression of beta-1,4-galactosyltransferase I, II, and V in human astrocytoma.
Herpes Zoster
Characterization of a novel ZP3-independent sperm-binding ligand that facilitates sperm adhesion to the egg coat.
Herpes Zoster
Molecular basis of mammalian gamete binding.
Hydrocephalus
Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId.
Joint Instability
Dwarfism with joint laxity in Friesian horses is associated with a splice site mutation in B4GALT7.
Joint Instability
Severe Peripheral Joint Laxity is a Distinctive Clinical Feature of Spondylodysplastic-Ehlers-Danlos Syndrome (EDS)-B4GALT7 and Spondylodysplastic-EDS-B3GALT6.
Lung Neoplasms
Elevated beta1,4-galactosyltransferase I in highly metastatic human lung cancer cells. Identification of E1AF as important transcription activator.
Melanoma
The level of cell surface beta1,4-galactosyltransferase I influences the invasive potential of murine melanoma cells.
Muscle Hypotonia
Severe Peripheral Joint Laxity is a Distinctive Clinical Feature of Spondylodysplastic-Ehlers-Danlos Syndrome (EDS)-B4GALT7 and Spondylodysplastic-EDS-B3GALT6.
Muscular Diseases
Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId.
Neoplasms
Correlated gene expression between beta-1,4-galactosyltransferase V and N-acetylglucosaminyltransferase V in human cancer cell lines.
Neoplasms
Galactosyltransferase associated with tumor in patients with ovarian cancer: factors involved in elevation of serum galactosyltransferase.
Neoplasms
Identification of beta-1,4-galactosyltransferase I as a target gene of HBx-induced cell cycle progression of hepatoma cell.
Neoplasms
Sequential action of Ets-1 and Sp1 in the activation of the human beta-1,4-galactosyltransferase V gene involved in abnormal glycosylation characteristic of cancer cells.
Neoplasms
The role of TNF-alpha and its receptors in the production of beta-1,4 galactosyltransferase I and V mRNAs by rat primary astrocytes.
Neoplasms
The role of TNF-alpha and its receptors in the production of beta-1,4-galactosyltransferase I mRNA by rat primary type-2 astrocytes.
Neoplasms
Transcriptional regulation of the human beta-1,4-galactosyltransferase V gene in cancer cells: essential role of transcription factor Sp1.
Neuroblastoma
Transcriptional regulation of the human beta-1,4-galactosyltransferase V gene in cancer cells: essential role of transcription factor Sp1.
Ovarian Neoplasms
Galactosyltransferase associated with tumor in patients with ovarian cancer: factors involved in elevation of serum galactosyltransferase.
Pectus Carinatum
Skeletal dysplasia, global developmental delay, and multiple congenital anomalies in a 5-year-old boy-Report of the second family with B3GAT3 mutation and expansion of the phenotype.
Refractive Errors
Corneal clouding, cataract, and colobomas with a novel missense mutation in B4GALT7-a review of eye anomalies in the linkeropathy syndromes.
Refractive Errors
Skeletal dysplasia, global developmental delay, and multiple congenital anomalies in a 5-year-old boy-Report of the second family with B3GAT3 mutation and expansion of the phenotype.
Retinal Detachment
Corneal clouding, cataract, and colobomas with a novel missense mutation in B4GALT7-a review of eye anomalies in the linkeropathy syndromes.
Skin Abnormalities
Skeletal dysplasia, global developmental delay, and multiple congenital anomalies in a 5-year-old boy-Report of the second family with B3GAT3 mutation and expansion of the phenotype.
Spinal Cord Injuries
Altered beta-1,4-galactosyltransferase I expression during early inflammation after spinal cord contusion injury.
Strabismus
Corneal clouding, cataract, and colobomas with a novel missense mutation in B4GALT7-a review of eye anomalies in the linkeropathy syndromes.
Tauopathies
Altered protein glycosylation predicts Alzheimer's disease and modulates its pathology in disease model Drosophila.
xylosylprotein 4-beta-galactosyltransferase deficiency
A genetic defect in the biosynthesis of dermatan sulfate proteoglycan: galactosyltransferase I deficiency in fibroblasts from a patient with a progeroid syndrome.
xylosylprotein 4-beta-galactosyltransferase deficiency
Expanding the clinical spectrum of B4GALT7 deficiency: homozygous p.R270C mutation with founder effect causes Larsen of Reunion Island syndrome.
xylosylprotein 4-beta-galactosyltransferase deficiency
Hypomorphic zebrafish models mimic the musculoskeletal phenotype of ?4GalT7-deficient Ehlers-Danlos syndrome.
xylosylprotein 4-beta-galactosyltransferase deficiency
Phenotype and response to growth hormone therapy in siblings with B4GALT7 deficiency.
xylosylprotein 4-beta-galactosyltransferase deficiency
Redefining the progeroid form of ehlers-danlos syndrome: Report of the fourth patient with B4GALT7 deficiency and review of the literature.
xylosylprotein 4-beta-galactosyltransferase deficiency
Report of two siblings with spondylodysplastic Ehlers-Danlos syndrome and B4GALT7 deficiency.
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0.39
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
0.58
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.025
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.034
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.75
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.53
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.75
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.35 - 1.06
4-methylumbelliferyl alpha-D-xylopyranoside
0.27 - 4.22
4-methylumbelliferyl O-beta-D-xylopyranoside
0.16 - 4.22
4-methylumbelliferyl-beta-D-xylopyranoside
0.33 - 0.5
4-methylumbelliferyl-beta-D-xyloside
1.27 - 7.93
4-nitrophenyl O-beta-D-xylopyranoside
0.85 - 7.93
4-nitrophenyl-beta-D-xylopyranoside
0.4
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.56
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.36
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.78
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.53
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.22 - 0.4
UDP-alpha-D-galactose
0.02 - 2.41
UDP-galactose
0.28
UDP-glucose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
0.13
UDP-xylose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
additional information
-
0.35
4-methylumbelliferyl alpha-D-xylopyranoside
wild type enzyme, at pH 7.0 and 37°C
0.35
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195R, at pH 7.0 and 37°C
0.44
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R226A, at pH 7.0 and 37°C
0.46
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R226K, at pH 7.0 and 37°C
0.54
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R270K, at pH 7.0 and 37°C
0.55
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195Q, at pH 7.0 and 37°C
0.59
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme Y199F, at pH 7.0 and 37°C
0.6
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R270A, at pH 7.0 and 37°C
0.64
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195A, at pH 7.0 and 37°C
1.06
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme Y196F, at pH 7.0 and 37°C
0.27
4-methylumbelliferyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.32
4-methylumbelliferyl O-beta-D-xylopyranoside
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.38
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.53
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.6
4-methylumbelliferyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C
1.76
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
2.26
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
2.99
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
3.26
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
4.22
4-methylumbelliferyl O-beta-D-xylopyranoside
mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.16
4-methylumbelliferyl-beta-D-xylopyranoside
membrane beta1,4-GalT7 in HeLa cells
0.16
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells
0.27
4-methylumbelliferyl-beta-D-xylopyranoside
maltose-binding protein-beta1,4-GalT7 fusion protein
0.27
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
0.32
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in HeLa cells
0.33
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
0.35
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
0.35
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195R
0.38
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells
0.44
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226A
0.46
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226K
0.52
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells
0.53
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells
0.54
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270K
0.55
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195Q
0.57
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells
0.59
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y199F
0.6
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270A
0.64
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195A
0.73
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells
0.74
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
1.06
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y196F
1.76
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells
2.26
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
2.57
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells
2.99
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
3.26
4-methylumbelliferyl-beta-D-xylopyranoside
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
4
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant W224H, value above 4, protein expressed in HeLa cells
4.22
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells
0.33
4-methylumbelliferyl-beta-D-xyloside
-
pH 5.5, 37°C after preincubation with ATP
0.5
4-methylumbelliferyl-beta-D-xyloside
-
pH 5.5, 37°C before preincubation with ATP
1.27
4-nitrophenyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
4.25
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
5.54
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
7.93
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.85
4-nitrophenyl-beta-D-xylopyranoside
membrane beta1,4-GalT7 in HeLa cells
0.85
4-nitrophenyl-beta-D-xylopyranoside
-
wild-type, pH and temperature not specified in the publication
1.27
4-nitrophenyl-beta-D-xylopyranoside
maltose-binding protein-beta1,4-GalT7 fusion protein
1.27
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
2.29
4-nitrophenyl-beta-D-xylopyranoside
-
mutant L206A, pH and temperature not specified in the publication
3.76
4-nitrophenyl-beta-D-xylopyranoside
-
mutant A186D, pH and temperature not specified in the publication
4.25
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
5.01
4-nitrophenyl-beta-D-xylopyranoside
-
mutant R270C, pH and temperature not specified in the publication
5.54
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
7.93
4-nitrophenyl-beta-D-xylopyranoside
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
0.22
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant wild-type enzyme
0.22
UDP-alpha-D-galactose
wild type enzyme, at pH 7.0 and 37°C
0.27
UDP-alpha-D-galactose
mutant enzyme R270A, at pH 7.0 and 37°C
0.27
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270A
0.29
UDP-alpha-D-galactose
mutant enzyme R226K, at pH 7.0 and 37°C
0.29
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226K
0.32
UDP-alpha-D-galactose
mutant enzyme H195R, at pH 7.0 and 37°C
0.32
UDP-alpha-D-galactose
mutant enzyme Y199F, at pH 7.0 and 37°C
0.32
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195R
0.32
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y199F
0.33
UDP-alpha-D-galactose
mutant enzyme H195Q, at pH 7.0 and 37°C
0.33
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195Q
0.34
UDP-alpha-D-galactose
mutant enzyme R226A, at pH 7.0 and 37°C
0.34
UDP-alpha-D-galactose
mutant enzyme Y196F, at pH 7.0 and 37°C
0.34
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226A
0.34
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y196F
0.37
UDP-alpha-D-galactose
mutant enzyme R270K, at pH 7.0 and 37°C
0.37
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270K
0.4
UDP-alpha-D-galactose
mutant enzyme H195A, at pH 7.0 and 37°C
0.4
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195A
0.02
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli
0.07
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in HeLa cells
0.07
UDP-galactose
mutant enzyme W224H, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.1
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
0.21
UDP-galactose
pH 7.0, 37°C, mutant W222A, protein expressed in HeLa cells
0.21
UDP-galactose
mutant enzyme W222A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.23
UDP-galactose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
0.23
UDP-galactose
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
0.23
UDP-galactose
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.28
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
0.29
UDP-galactose
-
wild-type, pH and temperature not specified in the publication
0.31
UDP-galactose
membrane beta1,4-GalT7 in HeLa cells
0.31
UDP-galactose
-
mutant L206A, pH and temperature not specified in the publication
0.31
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in HeLa cells
0.31
UDP-galactose
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.33
UDP-galactose
pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells
0.33
UDP-galactose
mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.36
UDP-galactose
pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells
0.36
UDP-galactose
mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.43
UDP-galactose
pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells
0.43
UDP-galactose
mutant enzyme D230A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.45
UDP-galactose
pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells
0.45
UDP-galactose
pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells
0.45
UDP-galactose
mutant enzyme G223A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.45
UDP-galactose
mutant enzyme W222F, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.45
UDP-galactose
-
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C
0.47
UDP-galactose
pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells
0.47
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells
0.47
UDP-galactose
mutant enzyme D228E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.47
UDP-galactose
mutant enzyme E227D, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.48
UDP-galactose
pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells
0.48
UDP-galactose
mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
0.49
UDP-galactose
-
mutant R270C, pH and temperature not specified in the publication
0.63
UDP-galactose
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
0.63
UDP-galactose
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.65
UDP-galactose
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
0.65
UDP-galactose
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.17
UDP-galactose
pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells
1.17
UDP-galactose
mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C
2.07
UDP-galactose
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
2.07
UDP-galactose
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
2.41
UDP-galactose
-
mutant A186D, pH and temperature not specified in the publication
0.34
UDPgalactose
-
pH 5.5, 37°C after preincubation with ATP
0.41
UDPgalactose
-
pH 5.5, 37°C before preincubation with ATP
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics, molecular docking simulations of the Michaelis complex
-
additional information
UDP-glucuronic acid
KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
-
KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
-
KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
-
KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
-
KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.7
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
152
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.6
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
2
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
30.2
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
47.2
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
41
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.13 - 4080
4-methylumbelliferyl-beta-D-xylopyranoside
5460
4-nitrophenyl-beta-D-xylopyranoside
recombinant maltose-binding protein-beta1,4-GalT7 fusion protein
78.7
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
64.3
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
2.7
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
3.1
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
106.3
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.5 - 1.93
UDP-alpha-D-galactose
0.028 - 5086
UDP-galactose
72
UDP-glucose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
3 - 6
UDP-xylose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
0.13
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
0.5
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y196F
0.77
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270A
0.81
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270K
0.89
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226A
1.21
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y199F
1.35
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226K
1.49
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195R
1.5
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
1.62
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195Q
1.79
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
1.93
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195A
4080
4-methylumbelliferyl-beta-D-xylopyranoside
recombinant maltose-binding protein-beta1,4-GalT7 fusion protein
0.5
UDP-alpha-D-galactose
mutant enzyme Y196F, at pH 7.0 and 37°C
0.5
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y196F
0.77
UDP-alpha-D-galactose
mutant enzyme R270A, at pH 7.0 and 37°C
0.77
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270A
0.81
UDP-alpha-D-galactose
mutant enzyme R270K, at pH 7.0 and 37°C
0.81
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270K
0.89
UDP-alpha-D-galactose
mutant enzyme R226A, at pH 7.0 and 37°C
0.89
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226A
1.21
UDP-alpha-D-galactose
mutant enzyme Y199F, at pH 7.0 and 37°C
1.21
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y199F
1.35
UDP-alpha-D-galactose
mutant enzyme R226K, at pH 7.0 and 37°C
1.35
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226K
1.49
UDP-alpha-D-galactose
mutant enzyme H195R, at pH 7.0 and 37°C
1.49
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195R
1.5
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant wild-type enzyme
1.51
UDP-alpha-D-galactose
wild type enzyme, at pH 7.0 and 37°C
1.62
UDP-alpha-D-galactose
mutant enzyme H195Q, at pH 7.0 and 37°C
1.62
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195Q
1.93
UDP-alpha-D-galactose
mutant enzyme H195A, at pH 7.0 and 37°C
1.93
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195A
0.028
UDP-galactose
-
wild-type, pH and temperature not specified in the publication
0.1
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli
0.46
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
1
UDP-galactose
-
DELTA1-81, mutant A186D, pH and temperature not specified in the publication
1
UDP-galactose
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.05
UDP-galactose
-
DELTA1-81, mutant R270C, pH and temperature not specified in the publication
1.05
UDP-galactose
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.06
UDP-galactose
-
DELTA1-81, mutant L206P, pH and temperature not specified in the publication
1.06
UDP-galactose
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.11
UDP-galactose
-
DELTA1-81, wild-type, pH and temperature not specified in the publication
1.11
UDP-galactose
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.14
UDP-galactose
-
wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C
1.52
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
5086
UDP-galactose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
-
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-mannose
-
kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl galactosamine
-
kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-N-acetyl glucosamine
-
kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
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6.92
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
52.1
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
64
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
58.8
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
54.7
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
148.5
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
85.7
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.47 - 4.17
4-methylumbelliferyl alpha-D-xylopyranoside
0.35 - 4.12
4-methylumbelliferyl O-beta-D-xylopyranoside
0.17 - 5.42
4-methylumbelliferyl-beta-D-xylopyranoside
0.13 - 1.32
4-nitrophenyl O-beta-D-xylopyranoside
265.7
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
84.3
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
7.5
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
3.97
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
286.8
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.47 - 7.08
UDP-alpha-D-galactose
0.48 - 5.43
UDP-galactose
0.47
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme Y196F, at pH 7.0 and 37°C
1.2
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R270A, at pH 7.0 and 37°C
1.42
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R270K, at pH 7.0 and 37°C
1.87
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R226A, at pH 7.0 and 37°C
1.88
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme Y199F, at pH 7.0 and 37°C
2.92
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme R226K, at pH 7.0 and 37°C
2.95
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195Q, at pH 7.0 and 37°C
3
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195A, at pH 7.0 and 37°C
4.03
4-methylumbelliferyl alpha-D-xylopyranoside
mutant enzyme H195R, at pH 7.0 and 37°C
4.17
4-methylumbelliferyl alpha-D-xylopyranoside
wild type enzyme, at pH 7.0 and 37°C
0.35
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.43
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
3.12
4-methylumbelliferyl O-beta-D-xylopyranoside
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
4.12
4-methylumbelliferyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.17
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
0.47
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y196F
1.2
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270A
1.42
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R270K
1.87
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226A
1.88
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant Y199F
2.92
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant R226K
2.95
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195Q
3
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195A
4.03
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant mutant H195R
4.17
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
5.42
4-methylumbelliferyl-beta-D-xylopyranoside
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
0.13
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.22
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
0.3
4-nitrophenyl O-beta-D-xylopyranoside
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.32
4-nitrophenyl O-beta-D-xylopyranoside
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.47
UDP-alpha-D-galactose
mutant enzyme Y196F, at pH 7.0 and 37°C
1.47
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y196F
2.32
UDP-alpha-D-galactose
mutant enzyme R270K, at pH 7.0 and 37°C
2.32
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270K
2.85
UDP-alpha-D-galactose
mutant enzyme R226A, at pH 7.0 and 37°C
2.85
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226A
3.07
UDP-alpha-D-galactose
mutant enzyme R270A, at pH 7.0 and 37°C
3.07
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R270A
4.05
UDP-alpha-D-galactose
mutant enzyme Y199F, at pH 7.0 and 37°C
4.05
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant Y199F
4.7
UDP-alpha-D-galactose
mutant enzyme R226K, at pH 7.0 and 37°C
4.7
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant R226K
4.85
UDP-alpha-D-galactose
mutant enzyme H195A, at pH 7.0 and 37°C
4.85
UDP-alpha-D-galactose
mutant enzyme H195R, at pH 7.0 and 37°C
4.85
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195A
4.92
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195Q
4.92
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant mutant H195R
7.08
UDP-alpha-D-galactose
wild type enzyme, at pH 7.0 and 37°C
7.08
UDP-alpha-D-galactose
pH 7.0, 37°C, recombinant wild-type enzyme
0.48
UDP-galactose
-
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.63
UDP-galactose
-
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
1.67
UDP-galactose
-
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
4.6
UDP-galactose
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli
4.82
UDP-galactose
-
wild type enzyme, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication
5
UDP-galactose
pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli
5.43
UDP-galactose
pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli
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D165E
the apparent Km values of the D165E mutant toward UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside are about 3.8 and 5.5fold, respectively, higher than the wild type enzyme
D228E
the mutant expressed in HeLa cells retains about 40% activity of the wild type enzyme
D230A
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
DELTA1-81
-
deletion construct is enzymatically active with Km values similar to wild-type towards donor and acceptor substrate, kcat higher compared to full-length wild-type
DELTA1-81/A186D
-
kcat values similar to wild-type DELTA1-81 construct, Km values towards donor and acceptor substrates increased compared to wild-type
DELTA1-81/L206P
-
in contrast to full-length mutant L206P this deletion mutant is enzymatically active, kcat values similar to wild-type DELTA1-81 construct, Km values towards donor and acceptor substrates increased compared to wild-type
DELTA1-81/R270C
-
kcat values similar to wild-type DELTA1-81 construct, Km values towards donor and acceptor substrates increased compared to wild-type
E227D
the mutant displays similar apparent Km values toward both donor and acceptor substrates compared with the wild type enzyme
F221A
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
F221Y
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
G223a
the mutant is about 40% less active than the wild type enzyme
G225A
the mutant does not display any in vitro activity
L206A
-
mutant shows similar enzymatic activity compared to wild-type, Km towards donor substrate similar to wild-type, increased towards acceptor substrate
V164A
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
W222A
the mutant shows decreased Km value towards UDP-galactose compared to the wild type enzyme
W222F
the mutation has no significant effect on the Km value toward donor or acceptor substrate
W224H
the mutant is able to sustain decorin glycosaminoglycan chain substitution but not glycosaminoglycan synthesis from exogenously added xyloside
Y306G/W307G
double-mutant deprives the galactosyltransferase activity of GalT I, the point mutation abolishes the ability of GalT I to promote cell cycle progression of hepatoma cells
A186D
-
mutant shows decreased enzymatic activity compared to wild-type, Km towards donor and acceptor substrate increased compared to wild-type
A186D
-
the mutant exhibits 9times reduced activity compared to the wild type enzyme
A186D
-
the mutation is associated with the progeroid form of the Ehlers-Danlos syndrome and with the reduction of enzyme activity. The mutation weakly impairs the binding of the donor substrate. Glycosaminoglycan biosynthesis is slightly affected by the mutation
D211N
inactive
D211N
site-directed mutagenesis, inactive mutant
H195A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H195A
the mutant shows reduced activity compared to the wild type enzyme
H195Q
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H195Q
the mutant shows reduced activity compared to the wild type enzyme
H195R
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H195R
the mutant shows reduced activity compared to the wild type enzyme
L206P
-
mutant is inactive
L206P
-
loss of activity upon L206P substitution due to altered protein folding is the primary cause for the glycosaminoglycan synthesis defect in patients with the progeroid form of Ehlers-Danlos syndrome carrying the compound
L206P
-
the mutation is associated with the progeroid form of the Ehlers-Danlos syndrome and with the reduction of enzyme activity. Glycosaminoglycan biosynthesis is fully inhibited by the mutation
R226A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
R226A
the mutant shows reduced activity compared to the wild type enzyme
R226K
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
R226K
the mutant shows reduced activity compared to the wild type enzyme
R270A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
R270A
the mutant shows reduced activity compared to the wild type enzyme
R270C
-
compared to control fibroblasts, galactosyltransferase activity in beta4GalT-7Arg270Cys cells is approximately three times reduced over a temperature range of 25-41°C. The reduced beta4GalT-7 activity results in abnormal extracellular matrix structure due to altered decorin and biglycan glycosylation and defective collagen
R270C
-
mutant shows similar enzymatic activity compared to wild-type, Km towards donor substrate weakly increased compared to wild-type and strongly increased towards acceptor substrate
R270C
-
the mutation is associated with the progeroid form of the Ehlers-Danlos syndrome and with the reduction of enzyme activity. The mutation strongly impairs the binding of the acceptor substrate. Glycosaminoglycan biosynthesis is decreased by the mutation
R270C
-
the substitution strongly reduces enzyme affinity towards xyloside acceptor to about 60% compared to the wild type enzyme, thus affecting glycosaminoglycan chains formation
R270K
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
R270K
the mutant shows reduced activity compared to the wild type enzyme
Y194A
inactive
Y194A
site-directed mutagenesis, inactive mutant
Y194F
inactive
Y194F
site-directed mutagenesis, inactive mutant
Y196A
inactive
Y196A
site-directed mutagenesis, inactive mutant
Y196F
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y196F
the mutant shows reduced activity compared to the wild type enzyme
Y199A
inactive
Y199A
site-directed mutagenesis, inactive mutant
Y199F
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y199F
the mutant shows reduced activity compared to the wild type enzyme
additional information
-
decorin is less efficiently substituted with glycosaminoglycan chains upon expression of beta4GalT-7(186D) compared to beta4GalT-7-expressing cells. Cells expressing beta4GalT-7206P do not synthesize the proteoglycan form of decorin
additional information
construction of a soluble enzyme mutant by truncation of the first 60 N-terminal amino acids
additional information
construction of truncated mutant beta4GalT7DELTA81
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Higuchi, T.; Tamura, S.; Takagaki, K.; Nakamura, T.; Morikawa, A.; Tanaka, K.; Tanaka, A.; Saito, Y.; Endo, M.
A method for determination of galactosyltransferase I activity synthesizing the proteoglycan linkage region
J. Biochem. Biophys. Methods
29
135-142
1994
Homo sapiens
brenda
Higuchi, T.; Tamura, S.; Tanaka, K.; Takagaki, K.; Saito, Y.; Endo, M.
Effects of ATP on regulation of galactosyltransferase-I activity responsible for synthesis of the linkage region between the core protein and glycosaminoglycan chains of proteoglycans
Biochem. Cell Biol.
79
159-164
2001
Homo sapiens
brenda
Okajima, T.; Yoshida, K.; Kondo, T.; Furukawa, K.
Human homolog of Caenorhabditis elegans sqv-3 gene is galactosyltransferase I involved in the biosynthesis of the glucosaminoglycan-protein linkage region of proteoglycans
J. Biol. Chem.
274
22915-22918
1999
Homo sapiens
brenda
Zhou, D.; Malissard, M.; Berger, E.G.; Hennet, T.
Secretion and purification of recombinant beta1-4 galactosyltransferase from insect cells using pFmel-protA, a novel transposition-based baculovirus transfer vector
Arch. Biochem. Biophys.
374
3-7
2000
Homo sapiens
brenda
Gulberti, S.; Lattard, V.; Fondeur, M.; Jacquinet, J.C.; Mulliert, G.; Netter, P.; Magdalou, J.; Ouzzine, M.; Fournel-Gigleux, S.
Phosphorylation and sulfation of oligosaccharide substrates critically influence the activity of human beta1,4-galactosyltransferase 7 (GalT-I) and beta1,3-glucuronosyltransferase I (GlcAT-I) involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans
J. Biol. Chem.
280
1417-1425
2005
Homo sapiens
brenda
Goette, M.; Kresse, H.
Defective glycosaminoglycan substitution of decorin in a patient with progeroid syndrome is a direct consequence of two point mutations in the galactosyltransferase I (beta4GalT-7) gene
Biochem. Genet.
43
65-77
2005
Homo sapiens
brenda
Seidler, D.G.; Faiyaz-Ul-Haque, M.; Hansen, U.; Yip, G.W.; Zaidi, S.H.; Teebi, A.S.; Kiesel, L.; Goette, M.
Defective glycosylation of decorin and biglycan, altered collagen structure, and abnormal phenotype of the skin fibroblasts of an Ehlers-Danlos syndrome patient carrying the novel Arg270Cys substitution in galactosyltransferase I (beta4GalT-7)
J. Mol. Med.
84
583-594
2006
Homo sapiens
brenda
Bakker, H.; Rouwendal, G.J.; Karnoup, A.S.; Florack, D.E.; Stoopen, G.M.; Helsper, J.P.; van Ree, R.; van Die, I.; Bosch, D.
An antibody produced in tobacco expressing a hybrid beta-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes
Proc. Natl. Acad. Sci. USA
103
7577-7582
2006
Homo sapiens (P15291), Homo sapiens
brenda
Goette, M.; Spillmann, D.; Yip, G.W.; Versteeg, E.; Echtermeyer, F.G.; van Kuppevelt, T.H.; Kiesel, L.
Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (beta4GalT-7) deficient form of Ehlers-Danlos syndrome
Hum. Mol. Genet.
17
996-1009
2008
Homo sapiens
brenda
Daligault, F.; Rahuel-Clermont, S.; Gulberti, S.; Cung, M.T.; Branlant, G.; Netter, P.; Magdalou, J.; Lattard, V.
Thermodynamic insights into the structural basis governing the donor substrate recognition by human beta1,4-galactosyltransferase 7
Biochem. J.
418
605-614
2009
Homo sapiens (Q9UBV7), Homo sapiens
brenda
Wei, Y.; Liu, D.; Zhou, F.; Ge, Y.; Xu, J.; Yun, X.; Gu, J.; Jiang, J.
Identification of beta-1,4-galactosyltransferase I as a target gene of HBx-induced cell cycle progression of hepatoma cell
J. Hepatol.
49
1029-1037
2008
Homo sapiens (P15291)
brenda
Rahuel-Clermont, S.; Daligault, F.; Piet, M.H.; Gulberti, S.; Netter, P.; Branlant, G.; Magdalou, J.; Lattard, V.
Biochemical and thermodynamic characterization of mutated beta1,4-galactosyltransferase 7 involved in the progeroid form of the Ehlers-Danlos syndrome
Biochem. J.
432
303-311
2010
Homo sapiens
brenda
Talhaoui, I.; Bui, C.; Oriol, R.; Mulliert, G.; Gulberti, S.; Netter, P.; Coughtrie, M.W.; Ouzzine, M.; Fournel-Gigleux, S.
Identification of key functional residues in the active site of human {beta}1,4-galactosyltransferase 7: a major enzyme in the glycosaminoglycan synthesis pathway
J. Biol. Chem.
285
37342-37358
2010
Homo sapiens (Q9UBV7)
brenda
Pasek, M.; Boeggeman, E.; Ramakrishnan, B.; Qasba, P.K.
Galectin-1 as a fusion partner for the production of soluble and folded human beta-1,4-galactosyltransferase-T7 in E. coli
Biochem. Biophys. Res. Commun.
394
679-684
2010
Homo sapiens
brenda
Bui, C.; Talhaoui, I.; Chabel, M.; Mulliert, G.; Coughtrie, M.W.; Ouzzine, M.; Fournel-Gigleux, S.
Molecular characterization of beta1,4-galactosyltransferase 7 genetic mutations linked to the progeroid form of Ehlers-Danlos syndrome (EDS)
FEBS Lett.
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3962-3968
2010
Homo sapiens
brenda
Garcia-Garcia, J.F.; Corrales, G.; Casas, J.; Fernandez-Mayoralas, A.; Garcia-Junceda, E.
Synthesis and evaluation of xylopyranoside derivatives as decoy acceptors of human beta-1,4-galactosyltransferase 7
Mol. Biosyst.
7
1312-1321
2011
Homo sapiens
brenda
Gu, J.; Fan, J.; Xu, Y.; Xie, Y.; Gong, T.; Kong, Y.
Regulatory function of beta1,4-galactosyltransferase I expression on Lewis-Y glycan and embryo implantation
Gene
562
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2015
Homo sapiens (P15291)
brenda
Tsutsui, Y.; Ramakrishnan, B.; Qasba, P.K.
Crystal structures of beta-1,4-galactosyltransferase 7 enzyme reveal conformational changes and substrate binding
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288
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2013
Drosophila melanogaster (Q9VBZ9), Homo sapiens (Q9UBV7)
brenda
Saliba, M.; Ramalanjaona, N.; Gulberti, S.; Bertin-Jung, I.; Thomas, A.; Dahbi, S.; Lopin-Bon, C.; Jacquinet, J.C.; Breton, C.; Ouzzine, M.; Fournel-Gigleux, S.
Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors
J. Biol. Chem.
290
7658-7670
2015
Homo sapiens (Q9UBV7)
brenda
Siegbahn, A.; Thorsheim, K.; Stahle, J.; Manner, S.; Hamark, C.; Persson, A.; Tykesson, E.; Mani, K.; Westergren-Thorsson, G.; Widmalm, G.; Ellervik, U.
Exploration of the active site of beta4GalT7: modifications of the aglycon of aromatic xylosides
Org. Biomol. Chem.
13
3351-3362
2015
Homo sapiens (Q9UBV7)
brenda
Thorsheim, K.; Willen, D.; Tykesson, E.; Stahle, J.; Praly, J.P.; Vidal, S.; Johnson, M.T.; Widmalm, G.; Manner, S.; Ellervik, U.
Naphthyl thio- and carba-xylopyranosides for exploration of the active site of beta-1,4-galactosyltransferase 7 (beta4GalT7)
Chemistry
23
18057-18065
2017
Homo sapiens (Q9UBV7)
brenda