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Information on EC 2.4.1.133 - xylosylprotein 4-beta-galactosyltransferase and Organism(s) Homo sapiens

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.133 xylosylprotein 4-beta-galactosyltransferase
IUBMB Comments
Involved in the biosynthesis of the linkage region of glycosaminoglycan chains as part of proteoglycan biosynthesis (chondroitin, dermatan and heparan sulfates). Requires Mn2+.
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
galt i, b4galt7, galactosyltransferase i, beta-1,4-galt i, beta1,4-galt, beta-1,4-galt v, beta1,4-galt-i, galt-i, beta-1,4-galactosyltransferase i, beta4galt7, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
B4GALT7
-
-
beta-1,4-galactosyltransferase
-
-
beta-1,4-galactosyltransferase 7
-
beta-1,4-galactosyltransferase I
-
beta-1,4-galactosyltransferase-T7
-
-
beta-1,4-GalT 7
-
-
beta1,4-galactosyltransferase 7
beta1,4-galactosyltransferase I
-
beta1,4-GalT
-
beta1,4-GalT-I
-
beta1,4-GalT7
-
-
beta4-galactosyltransferase 7
-
-
beta4Gal-T7
-
-
beta4galactosyltransferase I
-
-
beta4GalT-7
-
-
beta4GalT7
galactosyltransferase I
galactosyltransferase, uridine diphosphogalactose-xylose
-
-
-
-
GalT-I
-
-
hbeta4GalT7
-
UDP-D-galactose:D-xylose galactosyltransferase
-
-
-
-
UDP-D-galactose:xylose galactosyltransferase
-
-
-
-
UDP-galactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase
-
-
UDPgalactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase
-
-
-
-
xylosylprotein 4-beta-galactosyltransferase
-
-
xylosylprotein beta1,4-galactosyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + [protein]-3-O-(beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine
show the reaction diagram
catalytic mechanism, oveview
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase
Involved in the biosynthesis of the linkage region of glycosaminoglycan chains as part of proteoglycan biosynthesis (chondroitin, dermatan and heparan sulfates). Requires Mn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
52227-72-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl beta-D-xylopyranoside + UDP-alpha-D-galactose
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactose + (1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
UDP + ?
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + (1S,2R,3S,4S)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
UDP + ?
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 2-naphthyl 1,5-dithio-beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-1,5-dithio-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 2-naphthyl 5-thio-beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 2-naphthyl beta-D-xylopyranoside
UDP + 4-beta-D-galactosyl-2-naphthyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl beta-D-carbaxylopyranoside
UDP + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-beta-D-carbaxylopyranoside
show the reaction diagram
a 5a-carba-beta-xylopyranoside
-
-
?
UDP-alpha-D-galactose + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl beta-L-carbaxylopyranoside
UDP + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-beta-L-carbaxylopyranoside
show the reaction diagram
a 5a-carba-beta-xylopyranoside
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl alpha-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
UDP + ?
show the reaction diagram
the strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay. Molecular modeling of the enzyme's active site in the presence of UDP-alpha-D-galactose and 4-methylumbelliferyl-beta-D-xylopyranoside using the crystal structure of Drosophila melanogaster enzyme dbeta4GalT7, PDB code 4M4K, overview
-
-
?
UDP-alpha-D-galactose + beciparcil
UDP + 4-[(4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranosyl)thio]benzonitrile
show the reaction diagram
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-alpha-D-galactose + iliparcil
UDP + 4-ethyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranoside
show the reaction diagram
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-alpha-D-galactose + naphthalen-2-yl 1-thio-beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-1-thio-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + naphthalen-2-yl beta-D-xylopyranoside
UDP + naphthalen-2-yl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + naroparcil
UDP + 4-[4-[(4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranosyl)thio]benzoyl]benzonitrile
show the reaction diagram
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein]
UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + odiparcil
UDP + 4-methyl-2-oxo-2H-1-benzopyran-7-yl 4-O-beta-D-galactopyranosyl-5-thio-beta-D-xylopyranoside
show the reaction diagram
a 5-thio-beta-D-xylopyranoside
-
-
?
UDP-galactose + 2-(6-hydroxynaphthyl) beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + 3-acetamido-propyl beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + 3-benzamido-propyl beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + 3-hexanamido-propyl beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galacto-pyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranoyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
-
?
UDP-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
UDP + 4-nitrophenyl 4-O-beta-D-galacto-pyranosyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
-
?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
show the reaction diagram
UDP-galactose + N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
show the reaction diagram
UDP-galactose + O-gamma-2,3,4-tri-O-acetyl-(beta-D-xylopyranosyl)-N-Cbz-L-serine methyl ester
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-D-xyloside
UDP + Galbeta(1-4)Xylbeta-p-nitrophenyl
show the reaction diagram
-
-
-
-
?
UDP-glucose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
?
UDP-glucuronic acid + 4-methylumbelliferyl-beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
?
UDP-N-acetyl glucosamine + 4-methylumbelliferyl-beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
?
UDP-xylose + 4-methylumbelliferyl-beta-D-xylopyranoside
?
show the reaction diagram
-
-
-
?
UDPgalactose + 4-methylumbelliferyl-beta-D-xyloside
UDP + beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(4-methylumbelliferone)
show the reaction diagram
UDPgalactose + p-nitrophenyl beta-D-xylopyranoside
UDP + beta-D-galactopyranosyl-1,4-xylopyranosyl-1-O-nitrophenol
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-galactose + 2-naphthyl beta-D-xylopyranoside
UDP + 4-beta-D-galactosyl-2-naphthyl-beta-D-xylopyranoside
show the reaction diagram
-
-
-
?
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein]
UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
show the reaction diagram
-
-
-
?
UDP-galactose + O-beta-D-xylosylprotein
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
-
3-acetamido-propyl beta-D-xylopyranoside
-
-
3-benzamido-propyl beta-D-xylopyranoside
-
-
3-hexanamido-propyl beta-D-xylopyranoside
-
-
4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose
-
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside
-
4-methylumbelliferyl 4-deoxy-beta-D-xyloside
-
4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside
50% inhibition at 2 mM. The strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
-
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
-
naphthyl 4-deoxy-beta-D-xylopyranoside
slight inhibition
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
-
UDP-mannose
-
UDP-N-acetylgalactosamine
-
additional information
not inhibited by naphthyl 4-deoxy-beta-D-xylopyranoside; structure-guided design of enzyme inhibitors, xyloside inhibitors design and in vitro and in cellulo competition assays, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
results suggest that a protein kinase in the 100000 x g supernatant activates galactosyltransferase I
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.39
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
0.58
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.025
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.034
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.75
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.53
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.75
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.35 - 1.06
4-methylumbelliferyl alpha-D-xylopyranoside
0.27 - 4.22
4-methylumbelliferyl O-beta-D-xylopyranoside
0.16 - 4.22
4-methylumbelliferyl-beta-D-xylopyranoside
0.33 - 0.5
4-methylumbelliferyl-beta-D-xyloside
1.27 - 7.93
4-nitrophenyl O-beta-D-xylopyranoside
0.85 - 7.93
4-nitrophenyl-beta-D-xylopyranoside
0.4
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.56
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.36
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.78
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
0.53
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.22 - 0.4
UDP-alpha-D-galactose
0.02 - 2.41
UDP-galactose
0.28
UDP-glucose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
0.13
UDP-xylose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
0.34 - 0.41
UDPgalactose
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
152
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.6
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
2
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
30.2
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
47.2
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
41
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.13 - 4080
4-methylumbelliferyl-beta-D-xylopyranoside
5460
4-nitrophenyl-beta-D-xylopyranoside
recombinant maltose-binding protein-beta1,4-GalT7 fusion protein
78.7
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
64.3
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
2.7
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
3.1
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
106.3
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.5 - 1.93
UDP-alpha-D-galactose
0.028 - 5086
UDP-galactose
72
UDP-glucose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
3 - 6
UDP-xylose
in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7
additional information
UDP-glucuronic acid
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.92
(1R,2S,3R,4R)-4-(2-naphthyloxy)cyclohexan-1,2,3-triol
pH 6.2, 37°C, recombinant enzyme
52.1
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
64
2-naphthyl 1,5-dithio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
58.8
2-naphthyl 5-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
54.7
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
148.5
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
85.7
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.47 - 4.17
4-methylumbelliferyl alpha-D-xylopyranoside
0.35 - 4.12
4-methylumbelliferyl O-beta-D-xylopyranoside
0.17 - 5.42
4-methylumbelliferyl-beta-D-xylopyranoside
0.13 - 1.32
4-nitrophenyl O-beta-D-xylopyranoside
265.7
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
84.3
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
7.5
naphthalen-2-yl 1-thio-beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
3.97
naphthalen-2-yl beta-D-xylopyranoside
pH 6.2, 37°C, recombinant enzyme
286.8
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.47 - 7.08
UDP-alpha-D-galactose
0.48 - 5.43
UDP-galactose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
3.69
3-acetamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.04
3-benzamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
2.46
3-hexanamido-propyl beta-D-xylopyranoside
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
0.03
4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose
at pH 7.0 and 37°C
0.53
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
0.53
4-methylumbelliferyl 4-deoxy-beta-D-xyloside
at pH 7.0 and 37°C
0.03
4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside
pH 7.0, 37°C, recombinant wild-type enzyme
0.61
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
3.96
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
2.39
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C
1.93
TDP
UDP-galactose 0.1-16 mM, TDP 0-4 mM
0.61
UDP
UDP-galactose 0.1-16 mM, UDP 0-2 mM
1.65
UTP
UDP-galactose 0.1-16 mM, UTP 0-4 mM
additional information
CDP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose
Homo sapiens
at pH 7.0 and 37°C
1.28
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside
Homo sapiens
pH 7.0, 37°C, recombinant wild-type enzyme
1.28
4-methylumbelliferyl 4-deoxy-beta-D-xyloside
Homo sapiens
at pH 7.0 and 37°C
0.06
4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside
Homo sapiens
pH 7.0, 37°C, recombinant wild-type enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.058
maltose-binding protein-fusion enzyme, supernatant, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0
0.97
maltose-binding protein-fusion enzyme, amylose elution, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0
1.9
-
the protA-tagged beta4GalT
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
human uterine epithelial cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
beta-1,4-galactosyltransferase 7 (beta4GalT7) is a key enzyme in the biosynthesis of glycosaminoglycan chains
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
B4GT7_HUMAN
327
1
37406
Swiss-Prot
Mitochondrion (Reliability: 3)
B4GT1_HUMAN
398
1
43920
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
SDS-PAGE
39000
70000
recombinant fusion protein, by SDS-PAGE
71000
-
x * 71000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
the human enzyme presents only one potential glycosylation site at Asn154
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant detagged wild-type and truncated enzymes, hanging drop vapor diffusion method, mixing of 15 mg/ml protein solution with reservoir solution containing 100 mM imidazole, pH 6.5, 5 mM MnCl2, and 750 mM sodium acetate, 18°C, X-ray diffraction structure determination and analysis, molecular replacement using the Drosophila melanogaster beta4GalT7 crystal structure, PDB ID 3LW6, as a search model. For crystal complexes, tetragonal crystals are soaked in the same pH 6.5 reservoir solution containing 15% 2-methyl-2,4-pentanediol at 18 °C for 20 h followed by a brief soak in 100 mM Tris, pH 8.0, 750 mM sodium acetate, 15% 2-methyl-2,4-pentanediol, 5 mM MnCl2, and 5 mM UDP-Gal at room temperature. The monoclinic crystals are obtained within 3 days using 100 mM Tris, pH 8.5, and 8% PEG 8000 as a reservoir solution by mixing it with 20 mg/ml beta4GalT7DELTA81 in 10 mM MES (pH 6.5), 150 mM NaCl, 5 mM MnCl2, and 5 mM UDP-galactose at 18 °C
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A186D
D163A
inactive
D165A
inactive
D165E
the apparent Km values of the D165E mutant toward UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside are about 3.8 and 5.5fold, respectively, higher than the wild type enzyme
D211N
D228A
inactive
D228E
the mutant expressed in HeLa cells retains about 40% activity of the wild type enzyme
D230A
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
DELTA1-81
-
deletion construct is enzymatically active with Km values similar to wild-type towards donor and acceptor substrate, kcat higher compared to full-length wild-type
DELTA1-81/A186D
-
kcat values similar to wild-type DELTA1-81 construct, Km values towards donor and acceptor substrates increased compared to wild-type
DELTA1-81/L206P
-
in contrast to full-length mutant L206P this deletion mutant is enzymatically active, kcat values similar to wild-type DELTA1-81 construct, Km values towards donor and acceptor substrates increased compared to wild-type
DELTA1-81/R270C
-
kcat values similar to wild-type DELTA1-81 construct, Km values towards donor and acceptor substrates increased compared to wild-type
E227D
the mutant displays similar apparent Km values toward both donor and acceptor substrates compared with the wild type enzyme
F221A
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
F221Y
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
G223a
the mutant is about 40% less active than the wild type enzyme
G225A
the mutant does not display any in vitro activity
H195A
H195Q
H195R
L206A
-
mutant shows similar enzymatic activity compared to wild-type, Km towards donor substrate similar to wild-type, increased towards acceptor substrate
L206P
R226A
R226K
R270A
R270C
R270K
V164A
the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme
W222A
the mutant shows decreased Km value towards UDP-galactose compared to the wild type enzyme
W222F
the mutation has no significant effect on the Km value toward donor or acceptor substrate
W224H
the mutant is able to sustain decorin glycosaminoglycan chain substitution but not glycosaminoglycan synthesis from exogenously added xyloside
Y194A
Y194F
Y196A
Y196F
Y199A
Y199F
Y306G/W307G
double-mutant deprives the galactosyltransferase activity of GalT I, the point mutation abolishes the ability of GalT I to promote cell cycle progression of hepatoma cells
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography
glutathione-Sepharose column chromatography
lactose affinity column chromatography, UDP-agarose column chromatography, and amylose column chromatography
-
nickel-iminodiacetic acid column chromatography
-
purification of maltose-binding protein-beta1,4-GalT7 fusion protein with an amylose column
recombinant His6-GST-tagged enzyme by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme by affinity chromatography, and cleavage of the tag by TEV protease
recombinant soluble GST-tagged mutant enzyme from Escherichia coli strain BL21(DE3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression of human membrane beta1,4-GalT7 in HeLa cells, expression of soluble form as a fusion protein with maltose-binding protein in Escherichia coli
expressed as a GST-fusion protein in Escherichia coli and as Myc-tagged fusion proteins in HeLa cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells, HeLa cells and CHO pgsB-618 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in in CHO618 cells as membrane protein and in Escherichia coli as soluble protein fused to maltose-binding protein
-
expression in galactosyltransferase-deficient CHO618 cells
-
expression in Spodoptera frugiperda SF9 cells as prot-A-beta4-GalT fusion protein
-
expression of a hybrid enzyme (called xylGalT), consisting of the N-terminal domain of Arabidopsis thaliana xylosyltransferase and the catalytic domain of human beta-1,4-galactosyltransferase I (GalT) in tobacco causes a sharp reduction of N-glycans with potentially immunogenic corebound xylose (Xyl) and fucose (Fuc) residues
gene B4GALT7, recombinant expression of GST-tagged wild-type and mutant enzymes in CHOpgsB-618 cells, recombinant expression of the soluble GST-tagged mutant enzyme in Escherichia coli strain BL21(DE3)
gene B4GALT7, recombinant expression of His6-GST-tagged enzyme
gene B4GALT7, recombinant expression of N-terminally His6-tagged enzyme
mutated beta1,4-GalT7s are expressed as soluble maltose-binding protein fusions in which the N-terminal cytoplasmic domain, the transmembrane segment and the stem region, which precede the catalytic domain and correspond to amino acids 1-81, are deleted
-
quantitative enzyme expression analysis by RT-PCR
transient expression in mouse fibroblast L cells and chinese hamster ovary K-1 cells
-
truncated enzyme form is expressed in Escherichia coli BL21(DE3) cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
decreasing of the expression of GalT I in hepatoma cells reduce the ability of tumor formation in vivo and inhibits hepatitis B virus-encoded protein HBx-induced cell cycle progression
GalT I is highly expressed in hepatocellular carcinoma and transcriptionally up-regulated by hepatitis B virus-encoded HBx, and functions as a positive growth regulator in hepatoma cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
the enzyme is a target for the development of enzyme inhibitors as regulators of glycosaminoglycan synthesis in therapeutics
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Higuchi, T.; Tamura, S.; Takagaki, K.; Nakamura, T.; Morikawa, A.; Tanaka, K.; Tanaka, A.; Saito, Y.; Endo, M.
A method for determination of galactosyltransferase I activity synthesizing the proteoglycan linkage region
J. Biochem. Biophys. Methods
29
135-142
1994
Homo sapiens
Manually annotated by BRENDA team
Higuchi, T.; Tamura, S.; Tanaka, K.; Takagaki, K.; Saito, Y.; Endo, M.
Effects of ATP on regulation of galactosyltransferase-I activity responsible for synthesis of the linkage region between the core protein and glycosaminoglycan chains of proteoglycans
Biochem. Cell Biol.
79
159-164
2001
Homo sapiens
Manually annotated by BRENDA team
Okajima, T.; Yoshida, K.; Kondo, T.; Furukawa, K.
Human homolog of Caenorhabditis elegans sqv-3 gene is galactosyltransferase I involved in the biosynthesis of the glucosaminoglycan-protein linkage region of proteoglycans
J. Biol. Chem.
274
22915-22918
1999
Homo sapiens
Manually annotated by BRENDA team
Zhou, D.; Malissard, M.; Berger, E.G.; Hennet, T.
Secretion and purification of recombinant beta1-4 galactosyltransferase from insect cells using pFmel-protA, a novel transposition-based baculovirus transfer vector
Arch. Biochem. Biophys.
374
3-7
2000
Homo sapiens
Manually annotated by BRENDA team
Gulberti, S.; Lattard, V.; Fondeur, M.; Jacquinet, J.C.; Mulliert, G.; Netter, P.; Magdalou, J.; Ouzzine, M.; Fournel-Gigleux, S.
Phosphorylation and sulfation of oligosaccharide substrates critically influence the activity of human beta1,4-galactosyltransferase 7 (GalT-I) and beta1,3-glucuronosyltransferase I (GlcAT-I) involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans
J. Biol. Chem.
280
1417-1425
2005
Homo sapiens
Manually annotated by BRENDA team
Goette, M.; Kresse, H.
Defective glycosaminoglycan substitution of decorin in a patient with progeroid syndrome is a direct consequence of two point mutations in the galactosyltransferase I (beta4GalT-7) gene
Biochem. Genet.
43
65-77
2005
Homo sapiens
Manually annotated by BRENDA team
Seidler, D.G.; Faiyaz-Ul-Haque, M.; Hansen, U.; Yip, G.W.; Zaidi, S.H.; Teebi, A.S.; Kiesel, L.; Goette, M.
Defective glycosylation of decorin and biglycan, altered collagen structure, and abnormal phenotype of the skin fibroblasts of an Ehlers-Danlos syndrome patient carrying the novel Arg270Cys substitution in galactosyltransferase I (beta4GalT-7)
J. Mol. Med.
84
583-594
2006
Homo sapiens
Manually annotated by BRENDA team
Bakker, H.; Rouwendal, G.J.; Karnoup, A.S.; Florack, D.E.; Stoopen, G.M.; Helsper, J.P.; van Ree, R.; van Die, I.; Bosch, D.
An antibody produced in tobacco expressing a hybrid beta-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes
Proc. Natl. Acad. Sci. USA
103
7577-7582
2006
Homo sapiens (P15291), Homo sapiens
Manually annotated by BRENDA team
Goette, M.; Spillmann, D.; Yip, G.W.; Versteeg, E.; Echtermeyer, F.G.; van Kuppevelt, T.H.; Kiesel, L.
Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (beta4GalT-7) deficient form of Ehlers-Danlos syndrome
Hum. Mol. Genet.
17
996-1009
2008
Homo sapiens
Manually annotated by BRENDA team
Daligault, F.; Rahuel-Clermont, S.; Gulberti, S.; Cung, M.T.; Branlant, G.; Netter, P.; Magdalou, J.; Lattard, V.
Thermodynamic insights into the structural basis governing the donor substrate recognition by human beta1,4-galactosyltransferase 7
Biochem. J.
418
605-614
2009
Homo sapiens (Q9UBV7), Homo sapiens
Manually annotated by BRENDA team
Wei, Y.; Liu, D.; Zhou, F.; Ge, Y.; Xu, J.; Yun, X.; Gu, J.; Jiang, J.
Identification of beta-1,4-galactosyltransferase I as a target gene of HBx-induced cell cycle progression of hepatoma cell
J. Hepatol.
49
1029-1037
2008
Homo sapiens (P15291)
Manually annotated by BRENDA team
Rahuel-Clermont, S.; Daligault, F.; Piet, M.H.; Gulberti, S.; Netter, P.; Branlant, G.; Magdalou, J.; Lattard, V.
Biochemical and thermodynamic characterization of mutated beta1,4-galactosyltransferase 7 involved in the progeroid form of the Ehlers-Danlos syndrome
Biochem. J.
432
303-311
2010
Homo sapiens
Manually annotated by BRENDA team
Talhaoui, I.; Bui, C.; Oriol, R.; Mulliert, G.; Gulberti, S.; Netter, P.; Coughtrie, M.W.; Ouzzine, M.; Fournel-Gigleux, S.
Identification of key functional residues in the active site of human {beta}1,4-galactosyltransferase 7: a major enzyme in the glycosaminoglycan synthesis pathway
J. Biol. Chem.
285
37342-37358
2010
Homo sapiens (Q9UBV7)
Manually annotated by BRENDA team
Pasek, M.; Boeggeman, E.; Ramakrishnan, B.; Qasba, P.K.
Galectin-1 as a fusion partner for the production of soluble and folded human beta-1,4-galactosyltransferase-T7 in E. coli
Biochem. Biophys. Res. Commun.
394
679-684
2010
Homo sapiens
Manually annotated by BRENDA team
Bui, C.; Talhaoui, I.; Chabel, M.; Mulliert, G.; Coughtrie, M.W.; Ouzzine, M.; Fournel-Gigleux, S.
Molecular characterization of beta1,4-galactosyltransferase 7 genetic mutations linked to the progeroid form of Ehlers-Danlos syndrome (EDS)
FEBS Lett.
584
3962-3968
2010
Homo sapiens
Manually annotated by BRENDA team
Garcia-Garcia, J.F.; Corrales, G.; Casas, J.; Fernandez-Mayoralas, A.; Garcia-Junceda, E.
Synthesis and evaluation of xylopyranoside derivatives as decoy acceptors of human beta-1,4-galactosyltransferase 7
Mol. Biosyst.
7
1312-1321
2011
Homo sapiens
Manually annotated by BRENDA team
Gu, J.; Fan, J.; Xu, Y.; Xie, Y.; Gong, T.; Kong, Y.
Regulatory function of beta1,4-galactosyltransferase I expression on Lewis-Y glycan and embryo implantation
Gene
562
220-225
2015
Homo sapiens (P15291)
Manually annotated by BRENDA team
Tsutsui, Y.; Ramakrishnan, B.; Qasba, P.K.
Crystal structures of beta-1,4-galactosyltransferase 7 enzyme reveal conformational changes and substrate binding
J. Biol. Chem.
288
31963-31970
2013
Drosophila melanogaster (Q9VBZ9), Homo sapiens (Q9UBV7)
Manually annotated by BRENDA team
Saliba, M.; Ramalanjaona, N.; Gulberti, S.; Bertin-Jung, I.; Thomas, A.; Dahbi, S.; Lopin-Bon, C.; Jacquinet, J.C.; Breton, C.; Ouzzine, M.; Fournel-Gigleux, S.
Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors
J. Biol. Chem.
290
7658-7670
2015
Homo sapiens (Q9UBV7)
Manually annotated by BRENDA team
Siegbahn, A.; Thorsheim, K.; Stahle, J.; Manner, S.; Hamark, C.; Persson, A.; Tykesson, E.; Mani, K.; Westergren-Thorsson, G.; Widmalm, G.; Ellervik, U.
Exploration of the active site of beta4GalT7: modifications of the aglycon of aromatic xylosides
Org. Biomol. Chem.
13
3351-3362
2015
Homo sapiens (Q9UBV7)
Manually annotated by BRENDA team
Thorsheim, K.; Willen, D.; Tykesson, E.; Stahle, J.; Praly, J.P.; Vidal, S.; Johnson, M.T.; Widmalm, G.; Manner, S.; Ellervik, U.
Naphthyl thio- and carba-xylopyranosides for exploration of the active site of beta-1,4-galactosyltransferase 7 (beta4GalT7)
Chemistry
23
18057-18065
2017
Homo sapiens (Q9UBV7)
Manually annotated by BRENDA team