pathogen-inducible enzyme. Over-expression leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance
transgenic plants over-expressing the salicylate- and pathogen-inducible glucosyltransferase. Activity of TOGT and the subsequent accumulation of glucosylated coumarins represent an important step in the cascade of events resulting in confinement of viral pathogens
the enzyme shows activity towards a number of coumarins including umbelliferone, scopoletin, isoscopoletin and esculetin, and flavonoids including a flavone, a flavanone and chalcones. No activity is detected with compounds characterized by a single aromatic ring, i.e. simple and acidic phenols
pathogen-inducible enzyme. Over-expression leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance
transgenic plants over-expressing the salicylate- and pathogen-inducible glucosyltransferase. Activity of TOGT and the subsequent accumulation of glucosylated coumarins represent an important step in the cascade of events resulting in confinement of viral pathogens
the addition of Na+ to buffered standard assays at concentration 1 and 10 mM has a stimulating effect on the glucosylation of scopoletin to 110% and 128%, respectively
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TITLE OF PUBLICATION
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Infections
Ectopic expression of a UDP-glucose:phenylpropanoid glucosyltransferase leads to increased resistance of transgenic tobacco plants against infection with Potato Virus Y.
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C
while in Nicotiana tabacum, TOGT (tobacco UGT) glucosylates scopoletin after treatment with salicylate, fungal elicitors and the tobacco mosaic virus, enzymes NbUGT73A24 and NbUGT73A25 produce glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana
glycosyltransferases of family 1 are capable of transferring sugar moieties from activated sugar donors to a wide range of small lipophilic acceptors leading to an increase in water solubility, improved chemical stability and altered biological activity of the sugar conjugates, in plants, family 1 glycosyltransferases play an important role in metabolic homeostasis, regulation of phytohormone activities and detoxification of xenobiotics such as herbicides and pesticides
glycosyltransferases of family 1 are capable of transferring sugar moieties from activated sugar donors to a wide range of small lipophilic acceptors leading to an increase in water solubility, improved chemical stability and altered biological activity of the sugar conjugates, in plants, family l glycosyltransferases play an important role in metabolic homeostasis, regulation of phytohormone activities and detoxification of xenobiotics such as herbicides and pesticides
NbUGT73A24 produces glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana
NbUGT73A25 produces glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana
overexpression of UGT73A24 and UGT73A25 leads to a significant reduction in the level of the pathogen-responsive metabolites, including phenylalanine and tryptophan although the latter are not substrates of the encoded enzymes
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LITERATURE
DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A24 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates
DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A25 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates
generation of plants overexpressing the enzyme. Over-expression leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance
recombinant and mutant glycosyltransferase 71C1 is expressed as fusion protein tagged with a 26 kDa GST protein at the N-terminus in Escherichia coli BL21, production of a chimeric mutant derived from 2 Arabidopsis glucosyltransferases, 71C1 and 71C3, the chimera contains the N-terminal domain of 71C1 and the C-terminal domain of 71C3
recombinant and mutant glycosyltransferase 71C3 is expressed as fusion protein tagged with a 26 kDa GST protein at the N-terminus in Escherichia coli BL21, production of a chimeric mutant derived from 2 Arabidopsis glucosyltransferases, 71C1 and 71C3, the chimera contains the N-terminal domain of 71C1 and the C-terminal domain of 71C3
Ectopic expression of a UDP-glucose:phenylpropanoid glucosyltransferase leads to increased resistance of transgenic tobacco plants against infection with Potato Virus Y
Over-expression of a scopoletin glucosyltransferase in Nicotiana tabacum leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance