Information on EC 2.4.1.122 - glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase

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The expected taxonomic range for this enzyme is: Bilateria

EC NUMBER
COMMENTARY
2.4.1.122
-
RECOMMENDED NAME
GeneOntology No.
glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-alpha-D-galactose + glycoprotein N-acetyl-D-galactosamine = UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
; the non-reducing O-serine-linked N-acetylgalactosamine residues in mucin glycoproteins can act as acceptors
-
-
-
UDP-alpha-D-galactose + glycoprotein N-acetyl-D-galactosamine = UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
charge-charge interactions between enzyme and substrate
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
hexosyl group transfer
Q7K237
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
mucin core 1 and core 2 O-glycosylation
-
Mucin type O-Glycan biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:glycoprotein-N-acetyl-D-galactosamine 3-beta-D-galactosyltransferase
The non-reducing O-serine-linked N-acetylgalactosamine residues in mucin glycoproteins can act as acceptors.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3betaGal-T4
-, O75752
-
beta-galactosyltransferase
-
-
C1beta3GalT
Q7K237
-
C1GalT
Q9NS00
-
C1GalTA
Q7K237
-
Ce-T-synthase
Q18515
-
core 1 beta1,3-galactosyltransferase
Q7K237
-
core 1 beta1,3-galactosyltransferase
-
-
core 1 beta1,3-galactosyltransferase
-
-
core 1 beta3-Gal-T
Q18515
-
core 1 beta3-Gal-T
-
-
core 1 beta3-Gal-T
-
-
core 1 beta3-Gal-transferase
Q9NS00
-
core 1 beta3-Gal-transferase
-
-
core 1 galactosyltransferase A
Q7K237
-
core 1 GalT
Q7K237
-
core 1 synthase
-
-
core 1 transferase
-
-
core 1 UDP-alpha-galactose (UDP-Gal):GalNAca1-Ser/Thr beta1,3-galactosyltransferase
Q18515
-
core-1 beta3GalT
-
-
dC1beta3GalT1
Q7K237
-
galactosyltransferase
Q7JRF9, Q7K237, Q7K509
-
galactosyltransferase, uridine diphosphogalactose-mucin beta-(1-3)-
-
-
-
-
mucin-type core-1 beta1-3 galactosyltransferase
-
-
O-glycan core 1 UDPgalactose:N-acetyl-alpha-galactosaminyl-R beta3-galactosyltransferase
-
-
T-synthase
Q18515
-
T-synthase
Q7K237
-
T-synthase
-
-
UDP-Gal:GalNAc-alpha-Ser/Thr beta3-galactosyltransferase
-
-
UDP-Gal:glycoprotein-alpha-GalNAc beta3 galactosyltransferase
-
-
UDP-Gal:N-acetylgalactosaminide mucin:beta1,3-galactosyltransferase
-
-
UDP-Gal:N-acetylgalactosaminide mucin:beta1,3-galactosyltransferase
-
-
UDP-galactose:glycoprotein-alpha-GalNAc beta3-galactosyltransferase
-
-
uridine diphosphogalactose-mucin beta-(1-3)-galactosyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
97089-61-7
-
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
Q7JRF9, Q7K509
C1GalTA null mutant is lethal, mutant animals exhibit a striking morphogenetic defect in which the ventral nerve cord is greatly elongated and the brain hemispheres are misshapen
malfunction
-
oocyte-specific deletion at the primary follicle stage of T-synthase leads to a sustained increase in fertility mutant females ovulated 30-50% more eggs and has a sustained increase in litter size compared to controls, ovarian weights and follicle numbers are greater in mutants, but follicular apoptosis is not decreased, the number of follicles entering the growing pool is unaltered, but 3-week mutants ovulate fewer eggs, suggesting that increased fertility results from prolonged follicle development, T-synthase mutant ovaries also contain numerous multiple-oocyte follicles that appeared to form by adjacent, predominantly preantral, follicles joining
malfunction
-
mutant embryos show the loss of T antigens on the CNS and on a subset of hemocytes, 2 mutant lines, EY13370 and KG02976, have a P-element insertion in the CG9520 gene region, although the P-elements are located in the first intron of the gene in both lines, KG02976 homozygotes develop normally whereas some EY13370 homozygotes die during development and escaper homozygous EY13370 adult flies have abnormal legs, the levels of the enzyme transcripts in mutant third instar larvae homozygous for KG02976 or EY13370 are reduced to 60% and 15% of that of wild-type third instar larvae, terminal galactose of T antigen is defective in mutant embryos
malfunction
-
mutations in the chaperone Cosmc, which is encoded by the X-chromosome gene (Xq24) in humans, leads to loss of T-synthase activity and expression of the abnormal Tn (GalNAc1-Ser/Thr) and Sialyl Tn (NeuAc2-3GalNAc1-Ser/Thr) antigens, which are also known as tumor-associated carbohydrate antigens, Tn syndrome is a rare autoimmune disease in which subpopulations of blood cells in all lineages carry an incompletely glycosylated membrane
malfunction
-
abrogation of the enzyme promotes membrane protein internalization. Disruption of core 1 O-glycosyltransferase activity induces endocytosis and faster accumulation of cell surface proteins in cytoplasmic vesicles and induces clathrin-mediated endocytosis. Enzyme-deficient human corneal keratinocytes display plasma membrane invaginations on the apical surface
physiological function
Q7JRF9, Q7K509
C1GalTA is required for nervous system morphogenesis, C1GalTA is required for laminin O-glycosylation
physiological function
-
T-synthase generates core 1-derived O-glycans
physiological function
-
enzyme is responsible for the synthesis and function of T antigen (mucin-type O-glycan) in vivo
physiological function
-
mucin-type protein O-glycosylation
physiological function
-
T-synthase is the key beta3-galactosyltransferase essential for biosynthesis of core 1 O-glycans (Gal beta1-3GalNAc alpha1-Ser/Thr) in animal cell glycoproteins
physiological function
-
core 1 O-glycosylation is required to maintain transcellular barrier function. Core 1 O-glycans contribute to maintenance of apical barrier function on exposed mucosal surfaces by preventing clathrin-mediated endocytosis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + A-P-(N-acetyl-D-galactosaminyl)T-S-S-A
UDP + A-P-(beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl)T-S-S-A
show the reaction diagram
-
synthetic peptide
-
-
?
UDP-galactose + A-P-(N-acetyl-D-galactosaminyl)T-S-S-S
UDP + A-P-(beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl)T-S-S-S
show the reaction diagram
-
synthetic peptide
-
-
?
UDP-galactose + Ac-GHA-(GalNAcalpha1-)TSLPVTG-NH2
UDP + Ac-GHA-(Galbeta1-3GalNAcalpha1-)TSLPVTG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 750% activity in H292-cells, 550% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-SCSTGHA-(GalNAcalpha1-)TSG-NH2
UDP + Ac-SCSTGHA-(Galbeta-1-3GalNAcalpha1-)TSG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 50% activity in H292-cells, 40% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-STGHA-(GalNAcalpha1-)TSLPG-NH2
UDP + Ac-STGHA-(Galbeta1-3GalNAcalpha1-)TSLPG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 425% activity in H292-cells, 275% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-SVS-(GalNAcalpha1-)TGHATSG-NH2
UDP + Ac-SVS-(Galbeta1-3GalNAcalpha1-)TGHATSG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 300% activity in H292-cells, 450% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-TSSVS-(GalNAcalpha1-)TGHAG-NH2
UDP + Ac-TSSVS-(Galbeta1-3GalNAcalpha1-)TGHAG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 400% activity in H292-cells, 4000% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + asialo bovine submaxillary mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo bovine submaxillary mucin
show the reaction diagram
-
-
-
?
UDP-galactose + asialo ovine submaxillary mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo ovine submaxillary mucin
show the reaction diagram
-
-
-
?
UDP-galactose + asialo ovine submaxillary mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo ovine submaxillary mucin
show the reaction diagram
-
best substrate
-
?
UDP-galactose + asialo-agalacto-alpha1-acid glycoprotein
UDP + galactosyl-beta-1,3-asialo-agalacto-alpha1-acid glycoprotein
show the reaction diagram
-
-
-
-
?
UDP-galactose + asialo-agalacto-glycophorin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo-agalacto-glycophorin
show the reaction diagram
-
-
-
?
UDP-galactose + asialo-mucin of Cowper's gland
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo-mucin of Cowper's gland
show the reaction diagram
-
-
-
?
UDP-galactose + benzyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside
UDP + benzyl 2-(acetylamino)-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D-galactopyranoside
show the reaction diagram
-
pH 7.0, 2 mM, 100% activity in H292-cells, 100% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + GalNAcalpha-Ser-Cy5
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-galactose + GalNAcalpha1-O-phenyl
?
show the reaction diagram
Q18515
the enzyme is capable of utilizing UDP-Gal as the donor for the transfer of Gal residues to GalNAcalpha-1-O-phenyl and GalNAcalpha1-Ser/Thr for synthesizing core 1 structure Galbeta1-3GalNAcalpha1-R
-
-
?
UDP-galactose + ganglioside Gg3
UDP + galactosyl-beta-1,3-ganglioside Gg3
show the reaction diagram
-, O75752
i.e. GalNAcbeta1,4-Galbeta1,4-Glcbeta1-ceramide
-
?
UDP-galactose + ganglioside GM2
UDP + galactosyl-beta-1,3-ganglioside GM2
show the reaction diagram
-
-
-
-
?
UDP-galactose + ganglioside GM2
UDP + galactosyl-beta-1,3-ganglioside GM2
show the reaction diagram
-, O75752
i.e. GalNAcbeta1,4-(NeuAcalpha-2,3-)Galbeta1,4-Glcbeta1-ceramide
i.e. ganglioside GM1
?
UDP-galactose + glycophorin
?
show the reaction diagram
-
-
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
Q96EU7, Q9NS00
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-, O75752
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
Q96EU7, Q9NS00
-
i.e. core 1 structure
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
linked to serine or threonine of the peptide/protein
i.e. core 1 structure
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
threonine alpha-D-galactosamine is preferred to serine alpha-D-galactosamine
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
Q9NS00
threonine alpha-D-galactosamine is preferred to serine alpha-D-galactosamine
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
Galbeta1,3-GalNAcalpha residues adjacent to Thr(GalNAc) reduces the activity
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
high specificity for N-acetylgalactosaminyl residues linked to serine or threonine
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
Q9NS00
high specificity for N-acetylgalactosaminyl residues linked to serine or threonine
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
most active acceptor: macromolecular mucin glycopeptides with free N-acetylgalactosyl residues linked to polypeptide chain
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
substrates with negatively charged amino acids on the N-terminal side are highly efficient substrates
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycopeptides with less than 5 amino acids containing terminal N-acetylgalactosaminyl residues
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosyl acceptor specificity study
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosyl acceptor specificity study
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosyl acceptor specificity study
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosyl acceptor specificity study
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosyl acceptor specificity study
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
Q96EU7, Q9NS00
enzyme is important in O-glycan biosynthesis in digestive organs
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
major control factor in the biosynthesis of O-glycans
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
key enzyme in biosynthesis of core 1 O-glycans
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
involved in O-glycan biosynthesis
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
the enzyme can O-glycosylate the mucin-like domains of glycoproteins in a site-specific manner dependent on the sort of glycosylation/presence of glycan C-terminal from site of action, a position specific glycosylation pattern results, regulation, overview
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + immunglobulin A1
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
removal or substitution of the 3-OH group or removal of the 4-OH group abolishes enzyme activity, removal or substitution of the 6-OH reduces activity slightly leading to competititve substrates
-
?
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
?
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
Q9NS00
-
-
?
UDP-galactose + N-acetyl-D-galactosamine
UDP + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
?
UDP-galactose + N-acetyl-D-galactosamine
UDP + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + beta-D-galactosyl-1,3-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins
-
-
-
UDP-galactose + N-acetylgalactosaminitol
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminitol
show the reaction diagram
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-N-acetylgalactosamide
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-p-nitrophenyl
show the reaction diagram
-
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-N-acetylgalactosamide
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-p-nitrophenyl
show the reaction diagram
Q96EU7, Q9NS00
-
-
-
?
UDP-galactose + salivary gland mucin 81 N-acetyl-D-galactosamine
UDP + salivary gland mucin 81 D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
-, glycosylation pattern of the mucin, overview
-
-
?
UDP-galactose + TTTV-(GalNAcalpha1-)TPTPTG
UDP + TTTV-(Galbeta1-3GalNAcalpha1-)TPTPTG
show the reaction diagram
-
pH 7.0, 0.2 mM, 50% activity in H292-cells, 90% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + TTTVTP-(GalNAcalpha1-)TPTG
UDP + TTTVTP-(Galbeta1-3GalNAcalpha1-)TPTG
show the reaction diagram
-
pH 7.0, 0.2 mM, 110% activity in H292-cells, 60% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + TTTVTPTP-(GalNAcalpha1-)TG
UDP + TTTVTPTP-(Galbeta1-3GalNAcalpha1-)TG
show the reaction diagram
-
pH 7.0, 0.2 mM, 65% activity in H292-cells, 60% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
additional information
?
-
-, O75752
no acceptor substrates: ovalbumin, beta-N-acetyl-D-glucosaminyl-benzyl
-
-
-
additional information
?
-
-
poor substrates are low-molecular weight acceptors, e.g. N-acetylgalactosamine
-
-
-
additional information
?
-
-
poor substrates are low-molecular weight acceptors, e.g. N-acetylgalactosamine, no acceptor substrates: type A and type 0 blood group glycoprotein, ovomucoid, ovalbumin, and fetuin devoid of sialic acid and galactose, terminal alpha-2,6-linked sialic acid residues inhibit the enzyme
-
-
-
additional information
?
-
-
the presence of multiple core-1 beta3GalT genes in Drosophila melanogaster suggests an increased complexity of core-1 O-glycan expression, which is possibly related to multiple developmental and physiological functions attributable to this class of glycans
-
-
-
additional information
?
-
-
elevated preferences for Gly at the +1 position with moderately high preferences for Phe and Tyr in the +3 position relative to the acceptor Thr-O-GalNAc
-
-
-
additional information
?
-
Q7JRF9, Q7K237, Q7K509
enzyme elongates O-GalNAc by adding galactose in a beta1,3 linkage
-
-
-
additional information
?
-
-
core 1 synthase specific molecular chaperone is essential for the expression of functional enzyme
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + glycophorin
?
show the reaction diagram
-
-
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
Q96EU7, Q9NS00
enzyme is important in O-glycan biosynthesis in digestive organs
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
major control factor in the biosynthesis of O-glycans
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
key enzyme in biosynthesis of core 1 O-glycans
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
involved in O-glycan biosynthesis
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
the enzyme can O-glycosylate the mucin-like domains of glycoproteins in a site-specific manner dependent on the sort of glycosylation/presence of glycan C-terminal from site of action, a position specific glycosylation pattern results, regulation, overview
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins
-
-
-
UDP-galactose + salivary gland mucin 81 N-acetyl-D-galactosamine
UDP + salivary gland mucin 81 D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosylation pattern of the mucin, overview
-
-
?
additional information
?
-
-
the presence of multiple core-1 beta3GalT genes in Drosophila melanogaster suggests an increased complexity of core-1 O-glycan expression, which is possibly related to multiple developmental and physiological functions attributable to this class of glycans
-
-
-
additional information
?
-
-
elevated preferences for Gly at the +1 position with moderately high preferences for Phe and Tyr in the +3 position relative to the acceptor Thr-O-GalNAc
-
-
-
additional information
?
-
Q7JRF9, Q7K237, Q7K509
enzyme elongates O-GalNAc by adding galactose in a beta1,3 linkage
-
-
-
additional information
?
-
-
core 1 synthase specific molecular chaperone is essential for the expression of functional enzyme
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CaCl2
-
0.1 mM are included in assay medium
Mn2+
-
-
Mn2+
-
-
Mn2+
-
activation, 5-10 mM
Mn2+
-
required
MnCl2
-
20 mM are included in assay medium
MnCl2
-
20 mM are included in assay medium
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6-O-(4,4-azo)phenyl-GalNAc-alpha-benzyl
-
-
UDP
-
slight inhibition
alpha-Lactalbumin
-
-
-
additional information
-
proline on the C-terminal side of the peptide substrate adjacent to Thr(GalNAc) is inhibitory
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Cosmc
-
specific molecular chaperone
-
lactacystin
-
treatment with 0.01 mM lactacystin results in the increased expression of C1GalT protein
Triton X-100
-
activation and stabilization, unstable without
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.55
-
A-P-(N-acetyl-D-galactosaminyl)T-S-S-A
-
-
0.00035
-
asialo Cowper's gland mucin
-
-
-
0.09
-
asialo Cowper's gland mucin
-
calculated on the basis of terminal N-galactosaminyl residues
-
1.7
-
N-acetyl-alpha-D-galactosaminyl-benzyl
-
-
0.76
-
N-acetyl-alpha-D-galactosaminyl-phenyl
-
-
0.02
-
UDP-galactose
-
-
0.025
-
UDP-galactose
-
-
0.63
-
UDP-galactose
-
with N-acetyl-D-galactosaminyl-alpha-phenyl
5
-
asialo ovine submaxillary mucin
-
in terms of N-acetylgalactosamine equivalents
-
additional information
-
additional information
-
kinetic modeling, numerical simulation of in vivo Core 1 glycosylation pattern
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0000028
-
-, O75752
recombinant in Sf9 cells
0.000042
-
-
liver
0.000168
-
-
the extracts of EY13370 third instar larvae have a galactosyltransferase activity of 44% of that of wild-type using para-nitrophenyl-N-acetyl-alpha-galactosaminide as the acceptor
0.00088
-
-
microsomal membranes, substrate: N-acetyl-D-galactosaminyl-alpha-benzyl
0.00134
-
-
microsomal membranes, substrate: A-P-N-acetyl-D-galactosaminyl-T-S-S-S
0.00428
-
-
purified enzyme
0.0071
-
-
partially purified enzyme, substrate: N-acetyl-D-galactosaminyl-alpha-benzyl
0.0194
-
-
Golgi membranes
0.031
-
-
partially purified enzyme, substrate: A-P-N-acetyl-D-galactosaminyl-T-S-S-A
0.81
-
-
purified enzyme
3.43
-
-
purified enzyme
additional information
-
-
-
additional information
-
Q7JRF9, Q7K509
C1GalTA exhibits high activity on Tn and glycopeptide substrates, although it also exhibits weaker activity on glycolipids
additional information
-
-
T-synthase may utilize charge-charge interactions to modulate its activity, rejecting basic peptides over neutral and acidic peptide acceptors, T-synthase recognizes specific features of the peptide portion of the substrate, including overall charge and the presence of Gly, Phe, Tyr, and perhaps Pro at specific positions, up to four residues from the site of glycosylation, and indeed may use these features to modulate sites of Core 1 glycosylation
additional information
-
-
inactive HPC4-tagged soluble T-synthase regained activity within 5 min after the addition of chaprone 6His-sCosmc, at 37°C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
7
-
broad
6.5
-
-
assay at
6.8
-
-
assay at
6.9
-
-
-
7
-
-
assay at
7.2
-
-
assay at
additional information
-
-
pI: 6.40
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
7.9
-
-
6.5
7.4
Q96EU7, Q9NS00
assays at; assays at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
Q96EU7, Q9NS00
assay at; assay at; assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Q7JRF9, Q7K509
in embryo
Manually annotated by BRENDA team
-, O75752
low content; low content
Manually annotated by BRENDA team
-
during embryogenesis, in late embryos at stage 16
Manually annotated by BRENDA team
-
low content
Manually annotated by BRENDA team
-
human colon cancer cell line LSB
Manually annotated by BRENDA team
Q7JRF9, Q7K509
in larvae
Manually annotated by BRENDA team
-
high content
Manually annotated by BRENDA team
Q7JRF9, Q7K509
in larvae
Manually annotated by BRENDA team
-, O75752
low content
Manually annotated by BRENDA team
-
high content
Manually annotated by BRENDA team
Q96EU7, Q9NS00
;
Manually annotated by BRENDA team
-
high content
Manually annotated by BRENDA team
-
high content
Manually annotated by BRENDA team
-
high content
Manually annotated by BRENDA team
Q96EU7, Q9NS00
low content; low content
Manually annotated by BRENDA team
-
low content
Manually annotated by BRENDA team
Q96EU7, Q9NS00
;
Manually annotated by BRENDA team
Q96EU7, Q9NS00
-
Manually annotated by BRENDA team
Q96EU7, Q9NS00
-
Manually annotated by BRENDA team
-
low content
Manually annotated by BRENDA team
Q96EU7, Q9NS00
;
Manually annotated by BRENDA team
additional information
Q96EU7, Q9NS00
no endogenous activity in Jurkat and LSC, a colorectal cell line, cells
Manually annotated by BRENDA team
additional information
-
LSC cells do not express C1GalT
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
inner surface
Manually annotated by BRENDA team
Q96EU7, Q9NS00
-
-
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
additional information
-
type II transmembrane protein
-
Manually annotated by BRENDA team
additional information
Q96EU7, Q9NS00
type II transmembrane protein; type II transmembrane protein
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
39000
-
-
HPC4-tagged soluble T-synthase, determined by SDS-PAGE
48000
-
-
gel filtration
50000
-
-
SDS-PAGE
84000
86000
-
gel filtration
90000
-
-
gel filtration in the presence of Triton X-100
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 68000, SDS-PAGE
?
-
x * 42000, SDS-PAGE
monomer
-
1 * 82000, SDS-PAGE
monomer
-
1 * 42000-43000, SDS-PAGE and amino acid sequence determination
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stabilization by Triton X-100 or Nonidet P-40 required, unstable without
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3°C, 0.1% Triton X-100 and 0.1 mg/ml albumin, at least 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
solubilized with 1% Triton X-100 in 0.1 M NaCl
-
partial, recombinant soluble epitope-tagged enzyme from 293T cell medium
-
partial, solubilized with 3% Triton X-100
-
affinity chromatography on immobilized asialo-bovine submaxillary gland mucin
-
affinity chromatography on Sepharose 4B containing covalently bound mucin substrate
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DNA and amino acid sequence determination
-, Q9QH18
cloning of the C-terminal catalytic domain (amino acids 44-388) of C2GalTA into the vector pMT(1B) and transfected into S2 cells
Q7JRF9, Q7K509
expression as FLAG-tagged proteins in Sf9 insect cells
-
DNA and amino acid sequence determination and analysis, transient functional expression in human 293T cells as wild-type and soluble, i.e. secreted into the medium, epitope-tagged protein, stable functional expression of the soluble epitope-tagged enzyme in CHO-K1 and Lec1-CHO cells
-
DNA sequence determination, functional expression in human LSC cells; functional expression in human LSC cells
Q96EU7, Q9NS00
expressed in HEK-293T cells
-
expressed in wheat germ cell-free translation system
-
functional expression in Spodoptera frugiperda Sf9cells via baculovirus infection
-, O75752
soluble T-synthase is made by co-expressing N-terminal HPC4 epitope-tagged soluble T-synthase with wild-type full-length Cosmc in Hi-5 insect cells using the Baculovirus system
-
DNA and amino acid sequence determination
-, Q9JJ06
generation of mutant mice, oocyte-specific deletion of T-synthase
-
DNA and amino acid sequence determination
Q9JJ05
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the chaperone Cosmc promotes renaturation of denatured T-synthase in vitro, HPC4-tagged soluble T-synthase is denatured in 1 ml of 6 M guanidinium hydrochloride, pH 7.2 for 90 min at room temperature, the sample is diluted 100times in reconstitution buffer (10 mM HEPES, 150 mM NaCl, 12 mM MgCl2, pH 7.8), reconstitution is initiated by the addition of 6His-sCosmc and ATP where the final concentration is 2.27 microM and 5 mM, reaction is incubated for 45 min at room temperature, 6His-sCosmc can cause up to 75% restoration of heat-denatured HPC4-tagged soluble T-synthase activity depending on how long the protein is heat-treated, and that it can restore 30% of activity to HPC4-tagged soluble T-synthase denatured by guanidinium hydrochloride treatment
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
polymorphisms of the C1GALT1 gene are associated with the genetic susceptibility to IgA nephropathy in Chinese population