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Information on EC 2.3.3.9 - malate synthase and Organism(s) Pseudomonas aeruginosa

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.9 malate synthase
IUBMB Comments
The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.
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This record set is specific for:
Pseudomonas aeruginosa
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The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
malate synthase, malate synthase g, glyoxylate cycle enzyme, malate synthetase, malate synthase a, malate synthase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glyoxylate transacetase
-
-
-
-
glyoxylic transacetase
-
-
-
-
L-malate glyoxylate-lyase (CoA-acetylating)
-
-
-
-
malate synthase 1
-
-
-
-
malate synthase G
malate synthetase
-
-
-
-
malic synthetase
-
-
-
-
malic-condensing enzyme
-
-
-
-
MSG
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldol condensation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:glyoxylate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.
CAS REGISTRY NUMBER
COMMENTARY hide
9013-48-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + glyoxylate + H2O
(S)-malate + CoA
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
acetyl-CoA
-
-
0.07
glyoxylate
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
pyruvate
Pseudomonas aeruginosa
-
1 mM pyruvate: 40% activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
85% activity
7
-
87% activity
additional information
-
below pH 6: no activity remains, outside pH 7-10 the activity decreases rapidly
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
47.1% activity
32.5 - 40
-
more than 90% of activity
5
-
15.5% activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8G5JBV4_PSEAI
725
0
78605
TrEMBL
-
A0A8F9K7G7_PSEAI
725
0
78703
TrEMBL
-
A0A8G6WP22_PSEAI
725
0
78690
TrEMBL
-
A0A8G4M425_PSEAI
725
0
78602
TrEMBL
-
A0A5F1BTQ6_PSEAI
725
0
78660
TrEMBL
-
A0A8G5G4J0_PSEAI
725
0
78659
TrEMBL
-
A0A367LYE3_PSEAI
165
0
18202
TrEMBL
-
A0A8G2RI33_PSEAI
725
0
78674
TrEMBL
-
A0A8G2PG07_PSEAI
725
0
79699
TrEMBL
-
A0A2R3J4N5_PSEAI
725
0
78556
TrEMBL
-
A0A0C6EL94_PSEAI
725
0
78646
TrEMBL
-
A0A8G7LBI6_PSEAI
725
0
78646
TrEMBL
-
A0A0C7D2Q3_PSEAI
725
0
78632
TrEMBL
-
A0A8G5U6E8_PSEAI
725
0
78658
TrEMBL
-
A0A8G6F498_PSEAI
725
0
78626
TrEMBL
-
A0A8G5MVT0_PSEAI
725
0
78631
TrEMBL
-
A0A8G6QZE4_PSEAI
725
0
78604
TrEMBL
-
A0A8F9KKL5_PSEAI
725
0
78641
TrEMBL
-
A0A8G7HT99_PSEAI
725
0
78662
TrEMBL
-
A0A431XHR2_PSEAI
725
0
78577
TrEMBL
-
A0A8G3DE99_PSEAI
725
0
78602
TrEMBL
-
A0A8G6ZY90_PSEAI
725
0
78647
TrEMBL
-
A0A8B4Z973_PSEAI
725
0
78660
TrEMBL
-
A0A8G3TA36_PSEAI
725
0
78631
TrEMBL
-
A0A8G3IT83_PSEAI
726
0
79052
TrEMBL
-
A0A4P0TLL6_PSEAI
41
0
4477
TrEMBL
-
A0A367M1C9_PSEAI
117
0
13244
TrEMBL
-
A0A0A8R9T0_PSEAI
725
0
78618
TrEMBL
-
A0A233T093_PSEAI
725
0
78644
TrEMBL
-
A0A643ES10_PSEAI
725
0
78646
TrEMBL
-
A0A7M3B4V3_PSEAI
726
0
79237
TrEMBL
-
A0A367ME64_PSEAI
313
0
33316
TrEMBL
-
A0A8G6DMU0_PSEAI
725
0
78644
TrEMBL
-
A0A8F9KI58_PSEAI
725
0
78731
TrEMBL
-
A0A4P0TLV2_PSEAI
684
0
74262
TrEMBL
-
A0A6A9K126_PSEAI
725
0
78601
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
79400
-
x * 79400, predicted and observed (SDS-PAGE)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 79400, predicted and observed (SDS-PAGE)
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 2
-
1 h preincubation: about 5% activity
702873
11
-
1 h preincubation: 90% activity
702873
5
-
1 h preincubation: about 5% activity
702873
6 - 7
-
1 h preincubation: 100% activity
702873
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
1 h preincubation: 100% activity
55
-
1 h preincubation: 47% activity
70
-
1 h preincubation: complete loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione affinity chromatography, proteolytic removal of the GST tag, anion exchange chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GST-tagged version expressed in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roucourt, B.; Minnebo, N.; Augustijns, P.; Hertveldt, K.; Volckaert, G.; Lavigne, R.
Biochemical characterization of malate synthase G of P. aeruginosa
BMC Biochem.
10
20
2009
Pseudomonas aeruginosa
Manually annotated by BRENDA team