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Information on EC 2.3.3.16 - citrate synthase (unknown stereospecificity) and Organism(s) Dictyostelium discoideum

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IUBMB Comments
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
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Dictyostelium discoideum
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Word Map
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The taxonomic range for the selected organisms is: Dictyostelium discoideum
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
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acetyl-CoA
+
H2O
+
oxaloacetate
=
citrate
+
CoA
+
+
=
+
Synonyms
citrate synthetase, mitochondrial citrate synthase, peroxisomal citrate synthase, si-citrate synthase, type ii citrate synthase, citrate synthase cit1, bifunctional citrate synthase/2-methylcitrate synthase, citrate condensing enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
citrate condensing enzyme
-
-
-
-
citrate oxaloacetate-lyase (CoA-acetylating)
-
-
-
-
citrate oxaloacetate-lyase, CoA-acetylating
-
-
-
-
citrate synthase
-
-
-
-
citrate synthetase
-
-
-
-
citric synthase
-
-
-
-
citric-condensing enzyme
-
-
-
-
citrogenase
-
-
-
-
oxalacetic transacetase
-
-
-
-
oxaloacetate transacetase
-
-
-
-
synthase, citrate
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + H2O + oxaloacetate = citrate + CoA
show the reaction diagram
ordered sequential mechanism
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
CAS REGISTRY NUMBER
COMMENTARY hide
9027-96-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
show the reaction diagram
-
key enzyme of tricarboxylic acid cycle
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
-
activates at at least 0.5 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
-
no inhibition
acetyl-CoA
-
no inhibition
ATP
-
no inhibition
CoA
-
competitive with acetyl-CoA, noncompetitive with oxaloacetate
NADH
-
no effect
oxaloacetate
-
no inhibition
p-chloromercuribenzoate
-
can be restored by dithiothreitol treatment
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ammonium sulfate
-
activates at 0.5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
acetyl-CoA
-
-
0.007
oxaloacetate
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
471.7
-
partially purified enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
enzyme assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CISYC_DICDI
512
0
58291
Swiss-Prot
other Location (Reliability: 1)
CISYM_DICDI
460
0
51209
Swiss-Prot
Mitochondrion (Reliability: 3)
CISYP_DICDI
492
0
55749
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
destabilization by all salts, exception is ammonium sulfate, which stabilizes at low concentration
-
dithiothreitol stabilizes for about 5 days
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, below, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol and 5 mg/ml bovine serum albumin, several months
-
4°C, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol, 1 day
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Porter, J.S.; Wright, B.E.
Partial purification and characterization of citrate synthase from Dictyostelium discoideum
Arch. Biochem. Biophys.
181
155-163
1977
Dictyostelium discoideum, Dictyostelium discoideum NC-4 / ATCC 24697
Manually annotated by BRENDA team