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Information on EC 2.3.2.B13 - ubiquitin transferase RING E3 (cullin-dependent) and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC2.3.2.B13
preliminary BRENDA-supplied EC number
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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.B13 ubiquitin transferase RING E3 (cullin-dependent)
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Homo sapiens
Reaction Schemes
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine
+
[acceptor protein]-L-lysine
=
[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine
+
N6-ubiquitinyl-[acceptor protein]-L-lysine
Synonyms
cullin-ring e3 ubiquitin ligase, cul2 e3 ligase, cullin 2 e3 ubiquitin ligase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cul2 E3 ligase
-
cullin 2 E3 ubiquitin ligase
-
cullin-RING E3 ubiquitin ligase
SYSTEMATIC NAME
IUBMB Comments
[cullin-RING-E3-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming)
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine
[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine
[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
VHL_HUMAN
213
0
24153
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
N-terminal domain in complex with von Hippel-Lindau tumor suppressor protein, elonhin B, and elongin C, hanging drop vapor diffusion method, using 5 M sodium formate as precipitant
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, glutatione S-transferase column chromatography, maltose binding protein-Trap column chromatography, anion exchange chromatorgaphy, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhao, B.; Zhang, K.; Villhauer, E.; Bhuripanyo, K.; Kiyokawa, H.; Schindelin, H.; Yin, J.
Phage display to identify Nedd8-mimicking peptides as inhibitors of the Nedd8 transfer cascade
ChemBioChem
14
1323-1330
2013
Homo sapiens
Manually annotated by BRENDA team
Nguyen, H.; Yang, H.; Fribourgh, J.; Wolfe, L.; Xiong, Y.
Insights into cullin-RING E3 ubiquitin ligase recruitment: Structure of the VHL-EloBC-Cul2 complex
Structure
23
441-449
2015
Homo sapiens (P40337)
Manually annotated by BRENDA team