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Information on EC 2.3.2.27 - RING-type E3 ubiquitin transferase and Organism(s) Rattus norvegicus

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.27 RING-type E3 ubiquitin transferase
IUBMB Comments
RING E3 ubiquitin transferases serve as mediators bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) and an acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. Unlike EC 2.3.2.26, HECT-type E3 ubiquitin transferase, the RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin. Many members of the RING-type E3 ubiquitin transferase family are not able to bind a substrate directly, and form a complex with a cullin scaffold protein and a substrate recognition module (the complexes are named CRL for Cullin-RING-Ligase). In these complexes, the RING-type E3 ubiquitin transferase provides an additional function, mediating the transfer of a NEDD8 protein from a dedicated E2 carrier to the cullin protein (see EC 2.3.2.32, cullin-RING-type E3 NEDD8 transferase). cf. EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
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This record set is specific for:
Rattus norvegicus
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Word Map
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E2 ubiquitin-conjugating enzyme]-L-cysteine
+
[acceptor protein]-N6-ubiquitinyl-L-lysine
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E2 ubiquitin-conjugating enzyme]-L-cysteine
+
[acceptor protein]-N6-ubiquitinyl-L-lysine
Synonyms
brca1, parkin, e3 ubiquitin ligase, e3 ligase, c-cbl, ciap2, trim5alpha, rnf43, trim25, trim5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
microtubule-associated E3 ubiquitin ligase isoform 1
-
RanBP-type and C3HC4-type zinc finger-containing protein 1
-
ring finger protein 103
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein] ubiquitin transferase (isopeptide bond-forming; RING-type)
RING E3 ubiquitin transferases serve as mediators bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) and an acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. Unlike EC 2.3.2.26, HECT-type E3 ubiquitin transferase, the RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin. Many members of the RING-type E3 ubiquitin transferase family are not able to bind a substrate directly, and form a complex with a cullin scaffold protein and a substrate recognition module (the complexes are named CRL for Cullin-RING-Ligase). In these complexes, the RING-type E3 ubiquitin transferase provides an additional function, mediating the transfer of a NEDD8 protein from a dedicated E2 carrier to the cullin protein (see EC 2.3.2.32, cullin-RING-type E3 NEDD8 transferase). cf. EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[Ube2B]-L-cysteine + [March10a]-L-lysine
[Ube2B]-L-cysteine + N6-ubiquitinyl-[March10a]-L-lysine
show the reaction diagram
-
-
-
?
[BRCK1-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [BRCK1]-L-lysine
[BRCK1-ubiquitin-carrier protein]-L-cysteine + [BRCK1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
the RING1 finger plays an important role in the self-ubiquitination of RBCK1, leading to mono- and slightly diubiquitinated products. Self-ubiquitinated RBCK1 is processed by the proteasomal degradation
-
-
?
[BRE1B-ubiquitin-carrier protein UbcH8]-S-ubiquitinyl-L-cysteine + [syntaxin1]-L-lysine
[BRE1B-ubiquitin-carrier protein UbcH8]-L-cysteine + [syntaxin1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
isoform BRE1B binds and recruits the brain-enriched E2 ubiquitin-conjugating enzyme UbcH8 to syntaxin 1 and facilitates the ubiquitination and proteasome-dependent degradation of syntaxin1
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
E3 ubiquitin ligase activity of isoform RBCK1 is inhibited by interaction with splice variant RBCK2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
abundantly expressed
Manually annotated by BRENDA team
abundantly expressed
Manually annotated by BRENDA team
abundantly expressed
Manually annotated by BRENDA team
abundantly expressed
Manually annotated by BRENDA team
March10 is specifically expressed in elongating and elongated spermatids
Manually annotated by BRENDA team
additional information
expression is absent in epididymal spermatozoa
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
membrane-associated
Manually annotated by BRENDA team
upon overexpression in COS7 cell
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
the RING-finger domain of March10a exhibits an E3 ubiquitin ligase activity along with the E2 ubiquitin-conjugating enzyme Ube2B. March10a undergoes proteasomal degradation by autoubiquitination in transfected COS7 cells, this activity is abolished upon microtubule disassembly
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MAEA_RAT
396
0
45336
Swiss-Prot
other Location (Reliability: 2)
PP1RB_RAT
127
0
13936
Swiss-Prot
other Location (Reliability: 1)
RN152_RAT
203
1
22356
Swiss-Prot
other Location (Reliability: 2)
RN157_RAT
682
0
74565
Swiss-Prot
other Location (Reliability: 2)
BRE1B_RAT
1002
0
113841
Swiss-Prot
other Location (Reliability: 2)
GOLI_RAT
419
2
46449
Swiss-Prot
Secretory Pathway (Reliability: 4)
LIN41_RAT
855
0
92125
Swiss-Prot
other Location (Reliability: 2)
MARHA_RAT
790
0
87886
Swiss-Prot
other Location (Reliability: 2)
MARHB_RAT
398
2
44085
Swiss-Prot
other Location (Reliability: 1)
NHLC1_RAT
396
0
42089
Swiss-Prot
other Location (Reliability: 2)
RN123_RAT
1318
0
149077
Swiss-Prot
Mitochondrion (Reliability: 5)
RN133_RAT
381
2
42872
Swiss-Prot
Secretory Pathway (Reliability: 2)
RNF5_RAT
180
2
19837
Swiss-Prot
other Location (Reliability: 1)
RNF8_RAT
487
0
55615
Swiss-Prot
other Location (Reliability: 5)
SH3R1_RAT
894
0
93879
Swiss-Prot
other Location (Reliability: 2)
SH3R2_RAT
735
0
79880
Swiss-Prot
other Location (Reliability: 5)
TIF1B_RAT
835
0
88956
Swiss-Prot
Mitochondrion (Reliability: 5)
UHRF1_RAT
774
0
87449
Swiss-Prot
other Location (Reliability: 3)
MARH2_RAT
246
2
27169
Swiss-Prot
other Location (Reliability: 2)
MARH3_RAT
253
2
28563
Swiss-Prot
other Location (Reliability: 2)
PJA2_RAT
707
0
77921
Swiss-Prot
other Location (Reliability: 1)
RN166_RAT
237
0
26064
Swiss-Prot
Mitochondrion (Reliability: 4)
RN167_RAT
349
1
38216
Swiss-Prot
Secretory Pathway (Reliability: 1)
RN168_RAT
564
0
64404
Swiss-Prot
other Location (Reliability: 4)
TRI63_RAT
351
0
39723
Swiss-Prot
other Location (Reliability: 2)
TRIM2_RAT
744
0
81427
Swiss-Prot
other Location (Reliability: 2)
PZRN3_RAT
1062
0
119289
Swiss-Prot
other Location (Reliability: 2)
RFFL_RAT
362
0
40409
Swiss-Prot
other Location (Reliability: 2)
RN135_RAT
415
0
46012
Swiss-Prot
other Location (Reliability: 4)
RN138_RAT
209
0
24072
Swiss-Prot
other Location (Reliability: 1)
RNF13_RAT
380
1
42585
Swiss-Prot
Secretory Pathway (Reliability: 1)
TRI17_RAT
477
0
54954
Swiss-Prot
other Location (Reliability: 1)
TRI18_RAT
667
0
75211
Swiss-Prot
other Location (Reliability: 3)
I2BPL_RAT
783
0
81496
Swiss-Prot
Mitochondrion (Reliability: 5)
MGRN1_RAT
533
0
58603
Swiss-Prot
other Location (Reliability: 2)
RN103_RAT
682
3
79019
Swiss-Prot
Secretory Pathway (Reliability: 1)
TRI40_RAT
247
0
28389
Swiss-Prot
other Location (Reliability: 1)
MYLIP_RAT
445
0
49890
Swiss-Prot
other Location (Reliability: 2)
RNF4_RAT
194
0
21897
Swiss-Prot
other Location (Reliability: 5)
TRI35_RAT
501
0
57304
Swiss-Prot
other Location (Reliability: 2)
TRI39_RAT
488
0
56394
Swiss-Prot
other Location (Reliability: 2)
UBR4_RAT
5194
0
573792
Swiss-Prot
other Location (Reliability: 1)
BARD1_RAT
768
0
84548
Swiss-Prot
Mitochondrion (Reliability: 4)
CBLB_RAT
938
0
104652
Swiss-Prot
other Location (Reliability: 2)
CBLC_RAT
497
0
55775
Swiss-Prot
other Location (Reliability: 5)
PRP19_RAT
504
0
55239
Swiss-Prot
other Location (Reliability: 2)
RING1_RAT
406
0
42661
Swiss-Prot
other Location (Reliability: 2)
RN146_RAT
352
0
38224
Swiss-Prot
other Location (Reliability: 2)
SIAH1_RAT
282
0
31137
Swiss-Prot
Mitochondrion (Reliability: 4)
SIAH2_RAT
325
0
34700
Swiss-Prot
Mitochondrion (Reliability: 4)
TRI13_RAT
407
1
46815
Swiss-Prot
other Location (Reliability: 2)
NEUL3_RAT
254
0
28014
Swiss-Prot
other Location (Reliability: 2)
RING2_RAT
308
0
34242
Swiss-Prot
other Location (Reliability: 2)
RN185_RAT
192
2
20493
Swiss-Prot
other Location (Reliability: 2)
RN187_RAT
236
0
26313
Swiss-Prot
other Location (Reliability: 3)
RNF34_RAT
381
0
42681
Swiss-Prot
other Location (Reliability: 5)
TRI50_RAT
483
0
54719
Swiss-Prot
other Location (Reliability: 2)
TRIM9_RAT
710
0
79206
Swiss-Prot
other Location (Reliability: 1)
TTC3_RAT
2000
0
225783
Swiss-Prot
other Location (Reliability: 4)
UBE4A_RAT
1066
0
122379
Swiss-Prot
other Location (Reliability: 2)
BRCA1_RAT
1817
0
199879
Swiss-Prot
other Location (Reliability: 2)
MARH7_RAT
692
0
76351
Swiss-Prot
Mitochondrion (Reliability: 3)
NSE1_RAT
266
0
30702
Swiss-Prot
Mitochondrion (Reliability: 2)
RN112_RAT
631
2
68646
Swiss-Prot
Mitochondrion (Reliability: 3)
RN114_RAT
229
0
25664
Swiss-Prot
other Location (Reliability: 3)
TRI23_RAT
573
0
63892
Swiss-Prot
other Location (Reliability: 5)
TRI26_RAT
542
0
62631
Swiss-Prot
other Location (Reliability: 2)
XIAP_RAT
496
0
56073
Swiss-Prot
other Location (Reliability: 1)
MIB2_RAT
971
0
105542
Swiss-Prot
other Location (Reliability: 1)
RN181_RAT
165
0
19288
Swiss-Prot
other Location (Reliability: 2)
RN182_RAT
247
2
27546
Swiss-Prot
other Location (Reliability: 2)
TRAF6_RAT
530
0
60253
Swiss-Prot
other Location (Reliability: 1)
TRI11_RAT
467
0
52554
Swiss-Prot
other Location (Reliability: 2)
TRI69_RAT
499
0
57215
Swiss-Prot
other Location (Reliability: 2)
D3ZSK3_RAT
419
0
46039
TrEMBL
other Location (Reliability: 2)
G3V930_RAT
317
0
35878
TrEMBL
other Location (Reliability: 2)
A0A0G2K048_RAT
619
0
67109
TrEMBL
Secretory Pathway (Reliability: 3)
Q5RJS4_RAT
266
0
30794
TrEMBL
other Location (Reliability: 3)
A0A8I5ZVF8_RAT
677
0
73893
TrEMBL
other Location (Reliability: 2)
A0A8I6AJL7_RAT
949
0
111418
TrEMBL
other Location (Reliability: 1)
D3ZBM2_RAT
1125
0
124823
TrEMBL
other Location (Reliability: 3)
A0A8I6GLE2_RAT
962
0
109211
TrEMBL
other Location (Reliability: 2)
D3ZNJ9_RAT
510
0
56945
TrEMBL
other Location (Reliability: 2)
M0R732_RAT
260
0
30094
TrEMBL
other Location (Reliability: 3)
D3ZV15_RAT
894
0
98193
TrEMBL
other Location (Reliability: 2)
A0A8I5ZKY1_RAT
286
3
33335
TrEMBL
Secretory Pathway (Reliability: 5)
F7FG68_RAT
608
6
66873
TrEMBL
Secretory Pathway (Reliability: 2)
Q566Q9_RAT
393
0
44469
TrEMBL
other Location (Reliability: 2)
B1WBX1_RAT
445
0
48310
TrEMBL
other Location (Reliability: 1)
A0A8I5ZNM0_RAT
239
0
26599
TrEMBL
other Location (Reliability: 5)
A0A8I6AD08_RAT
497
0
54548
TrEMBL
other Location (Reliability: 2)
A0A8I6GKG5_RAT
514
0
56260
TrEMBL
other Location (Reliability: 2)
A0A8I5ZLW1_RAT
353
1
39680
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8I6GK47_RAT
468
0
52026
TrEMBL
other Location (Reliability: 2)
A0A8I5XWV2_RAT
624
0
69581
TrEMBL
other Location (Reliability: 2)
D5MTG9_RAT
1757
0
200165
TrEMBL
other Location (Reliability: 1)
A0A8I6AQV5_RAT
279
0
30648
TrEMBL
other Location (Reliability: 4)
A0A140TAC8_RAT
970
0
105471
TrEMBL
other Location (Reliability: 1)
A0A858CAG1_RAT
244
0
27055
TrEMBL
other Location (Reliability: 2)
A0A0G2JYQ5_RAT
350
0
38149
TrEMBL
other Location (Reliability: 4)
A0A8I5ZWU2_RAT
802
0
88823
TrEMBL
other Location (Reliability: 2)
A0A0G2JX46_RAT
385
0
42436
TrEMBL
other Location (Reliability: 4)
A0A8I6GKR5_RAT
473
0
53667
TrEMBL
other Location (Reliability: 2)
A0JPN0_RAT
494
0
56009
TrEMBL
other Location (Reliability: 2)
A6P321_RAT
346
2
37555
TrEMBL
Secretory Pathway (Reliability: 5)
A0A0G2JT07_RAT
829
0
93223
TrEMBL
other Location (Reliability: 4)
A0A8I5ZN62_RAT
561
5
61375
TrEMBL
Secretory Pathway (Reliability: 2)
A0A8J8XDG1_RAT
664
0
74024
TrEMBL
other Location (Reliability: 2)
A0A8I6G5N6_RAT
902
0
102250
TrEMBL
other Location (Reliability: 2)
A0A8I6AG35_RAT
206
2
22800
TrEMBL
other Location (Reliability: 4)
A0A0G2K9L3_RAT
1149
0
127427
TrEMBL
other Location (Reliability: 3)
A0A8I6APD1_RAT
347
0
38168
TrEMBL
other Location (Reliability: 5)
A0A8I5ZS04_RAT
342
1
38552
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8I6AR04_RAT
1981
0
223940
TrEMBL
other Location (Reliability: 1)
B2RZ97_RAT
381
0
41805
TrEMBL
other Location (Reliability: 2)
D3Z9G0_RAT
567
0
64187
TrEMBL
other Location (Reliability: 2)
A0A8I6A649_RAT
714
0
79311
TrEMBL
other Location (Reliability: 1)
A0A0G2K7X2_RAT
610
0
69205
TrEMBL
other Location (Reliability: 2)
A0A8I6ABW2_RAT
1171
0
133447
TrEMBL
other Location (Reliability: 1)
A0A8I6AN80_RAT
110
0
12450
TrEMBL
other Location (Reliability: 2)
A0A140TA93_RAT
566
0
64663
TrEMBL
other Location (Reliability: 3)
A0A8I6AQV0_RAT
318
0
34849
TrEMBL
other Location (Reliability: 3)
D4A4T0_RAT
304
0
34886
TrEMBL
other Location (Reliability: 1)
A0A8I6ABF8_RAT
1963
0
221891
TrEMBL
other Location (Reliability: 1)
A0A8I5ZR20_RAT
1130
0
125442
TrEMBL
other Location (Reliability: 3)
A0A8I6AML0_RAT
782
2
86166
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8I6GLZ9_RAT
384
0
43144
TrEMBL
other Location (Reliability: 4)
A0A8I6ALB9_RAT
435
0
47672
TrEMBL
other Location (Reliability: 3)
A0A8J8XH25_RAT
297
0
33575
TrEMBL
other Location (Reliability: 2)
A0A8I6A0P9_RAT
332
0
37496
TrEMBL
other Location (Reliability: 1)
A0A8I6GM09_RAT
292
0
32122
TrEMBL
other Location (Reliability: 2)
D3ZS02_RAT
501
0
55545
TrEMBL
other Location (Reliability: 1)
A0A8I6AFF7_RAT
825
0
90889
TrEMBL
other Location (Reliability: 2)
Q68FR1_RAT
329
0
35047
TrEMBL
other Location (Reliability: 3)
A0A8I6AKX7_RAT
592
0
66540
TrEMBL
other Location (Reliability: 2)
A0A0G2K8K2_RAT
982
0
109265
TrEMBL
other Location (Reliability: 2)
A0A8I5Y675_RAT
1992
0
225242
TrEMBL
other Location (Reliability: 3)
A0A8I6A5I2_RAT
507
0
57270
TrEMBL
other Location (Reliability: 3)
A0A8I6AR28_RAT
558
0
63944
TrEMBL
other Location (Reliability: 2)
A0A8I6ABB7_RAT
226
0
25949
TrEMBL
other Location (Reliability: 1)
Q499Q1_RAT
328
0
35775
TrEMBL
other Location (Reliability: 2)
A0A0G2JTP7_RAT
336
0
37623
TrEMBL
other Location (Reliability: 2)
A0A8I5YBD2_RAT
449
0
50792
TrEMBL
other Location (Reliability: 2)
A0A8I5Y218_RAT
521
0
59101
TrEMBL
other Location (Reliability: 3)
D5MTH0_RAT
1755
0
199032
TrEMBL
other Location (Reliability: 1)
A0A8I5ZKQ5_RAT
704
0
80272
TrEMBL
other Location (Reliability: 4)
D3ZUV2_RAT
1006
0
110118
TrEMBL
other Location (Reliability: 5)
A0A0G2JWX8_RAT
282
0
30758
TrEMBL
other Location (Reliability: 1)
A0A8I6B298_RAT
399
0
45038
TrEMBL
other Location (Reliability: 1)
A0A8I6ABS6_RAT
968
0
109869
TrEMBL
other Location (Reliability: 1)
D4A9U6_RAT
1734
0
196336
TrEMBL
other Location (Reliability: 1)
F7IXA2_RAT
377
0
39377
TrEMBL
other Location (Reliability: 1)
D4A7F0_RAT
1889
0
212895
TrEMBL
other Location (Reliability: 3)
A0A8I6G861_RAT
870
0
96127
TrEMBL
other Location (Reliability: 2)
A0A8I6GIE7_RAT
376
0
42170
TrEMBL
other Location (Reliability: 4)
A0A8I6A316_RAT
320
0
34770
TrEMBL
Secretory Pathway (Reliability: 1)
A0A0G2K5V5_RAT
383
0
40051
TrEMBL
other Location (Reliability: 2)
A0A0U1ZF16_RAT
802
0
89173
TrEMBL
other Location (Reliability: 2)
Q6WDI9_RAT
1000
0
114513
TrEMBL
other Location (Reliability: 1)
D3ZYQ9_RAT
973
0
113460
TrEMBL
other Location (Reliability: 2)
A0A8I5ZKZ0_RAT
913
1
99225
TrEMBL
other Location (Reliability: 4)
A0A8I6A434_RAT
553
0
62960
TrEMBL
other Location (Reliability: 3)
F1LSY5_RAT
398
2
43926
TrEMBL
other Location (Reliability: 1)
A0A8I6AHQ3_RAT
379
0
42667
TrEMBL
other Location (Reliability: 1)
A0A8I6G5V3_RAT
477
0
53523
TrEMBL
other Location (Reliability: 5)
A0A8I6A4T5_RAT
248
0
27829
TrEMBL
other Location (Reliability: 3)
A0A8I6A4D7_RAT
631
0
68541
TrEMBL
other Location (Reliability: 5)
D3ZVH5_RAT
458
0
51051
TrEMBL
other Location (Reliability: 2)
G3V6K9_RAT
1040
0
119141
TrEMBL
other Location (Reliability: 4)
B5DFI5_RAT
513
0
58555
TrEMBL
other Location (Reliability: 3)
A0A8I6B5C4_RAT
913
1
98776
TrEMBL
other Location (Reliability: 4)
A0A0G2K2T3_RAT
1801
0
197964
TrEMBL
other Location (Reliability: 2)
A0A8I5ZYA3_RAT
578
6
63476
TrEMBL
Secretory Pathway (Reliability: 2)
F1M4M8_RAT
268
0
30766
TrEMBL
Secretory Pathway (Reliability: 5)
A0A0G2K1U4_RAT
1741
0
197130
TrEMBL
other Location (Reliability: 1)
A0A0U1ZEI3_RAT
196
0
21366
TrEMBL
other Location (Reliability: 1)
D3ZK36_RAT
803
0
90199
TrEMBL
other Location (Reliability: 3)
B1WCA3_RAT
233
0
26450
TrEMBL
other Location (Reliability: 5)
Q6TXG2_RAT
321
0
35085
TrEMBL
Secretory Pathway (Reliability: 1)
D3ZQC6_RAT
1249
0
141790
TrEMBL
other Location (Reliability: 2)
M0RDZ7_RAT
346
2
37556
TrEMBL
Secretory Pathway (Reliability: 5)
A0A0G2K4P8_RAT
298
0
33703
TrEMBL
other Location (Reliability: 2)
A0A8I5YBG5_RAT
1755
0
199017
TrEMBL
other Location (Reliability: 1)
F7FHL6_RAT
526
0
59523
TrEMBL
other Location (Reliability: 3)
M0R9Q8_RAT
653
0
70918
TrEMBL
other Location (Reliability: 2)
B2RYD5_RAT
619
0
67261
TrEMBL
other Location (Reliability: 4)
A0A8I5ZPA4_RAT
267
0
31059
TrEMBL
other Location (Reliability: 3)
D3ZNC9_RAT
409
2
45265
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8I6AFG2_RAT
411
0
44417
TrEMBL
other Location (Reliability: 2)
A0A8I6AQI2_RAT
264
0
29226
TrEMBL
other Location (Reliability: 2)
A0A8I6A812_RAT
931
0
108406
TrEMBL
other Location (Reliability: 2)
A0A8I6G786_RAT
925
0
105071
TrEMBL
other Location (Reliability: 1)
A0A8I5XVR7_RAT
1592
0
181081
TrEMBL
other Location (Reliability: 1)
A0A8I5Y6F4_RAT
239
2
28196
TrEMBL
other Location (Reliability: 4)
F7EMI5_RAT
412
0
46265
TrEMBL
other Location (Reliability: 3)
A0A8I6ARU4_RAT
542
0
61457
TrEMBL
other Location (Reliability: 2)
A0A8I5ZN06_RAT
238
0
25852
TrEMBL
other Location (Reliability: 4)
A0A8I5ZZN6_RAT
1757
0
200196
TrEMBL
other Location (Reliability: 1)
G3C8Z1_RAT
532
0
58532
TrEMBL
other Location (Reliability: 2)
G3V658_RAT
619
0
67245
TrEMBL
other Location (Reliability: 4)
A0A8I6A8T8_RAT
280
0
32004
TrEMBL
Mitochondrion (Reliability: 5)
M0R7W8_RAT
553
0
63233
TrEMBL
other Location (Reliability: 2)
A0A8I6ANE4_RAT
536
0
59727
TrEMBL
other Location (Reliability: 1)
A0A8I5YBW4_RAT
250
0
27664
TrEMBL
other Location (Reliability: 4)
A0A8I5ZTL8_RAT
982
0
106745
TrEMBL
other Location (Reliability: 1)
D3ZEH9_RAT
554
0
60969
TrEMBL
other Location (Reliability: 2)
A0A8I6ASX8_RAT
148
2
16488
TrEMBL
Mitochondrion (Reliability: 3)
Q99NN7_RAT
256
0
28885
TrEMBL
other Location (Reliability: 1)
M0RA58_RAT
627
0
68119
TrEMBL
other Location (Reliability: 5)
O70607_RAT
165
0
18942
TrEMBL
other Location (Reliability: 4)
A9UK92_RAT
282
0
31137
TrEMBL
Mitochondrion (Reliability: 4)
Q68AY0_RAT
333
0
38111
TrEMBL
other Location (Reliability: 1)
A0A0U1ZFN5_RAT
846
0
93387
TrEMBL
other Location (Reliability: 2)
A0A0G2JWJ3_RAT
735
0
83112
TrEMBL
other Location (Reliability: 3)
A0A8I5ZL10_RAT
729
0
81210
TrEMBL
other Location (Reliability: 1)
A0A0G2JW11_RAT
717
0
81473
TrEMBL
other Location (Reliability: 1)
A0A8I6G788_RAT
698
0
77654
TrEMBL
other Location (Reliability: 2)
A0A0G2JVC1_RAT
434
0
48699
TrEMBL
other Location (Reliability: 2)
A0A8I6A2Q1_RAT
808
1
88060
TrEMBL
other Location (Reliability: 1)
A0A8I6AI19_RAT
379
0
41555
TrEMBL
other Location (Reliability: 2)
A0A0G2K039_RAT
614
0
66745
TrEMBL
other Location (Reliability: 4)
A0A8I6A4G2_RAT
1626
0
182676
TrEMBL
other Location (Reliability: 1)
M0R6D9_RAT
878
0
93248
TrEMBL
Secretory Pathway (Reliability: 5)
Q5RKH8_RAT
521
0
59028
TrEMBL
other Location (Reliability: 3)
D3ZI70_RAT
247
0
28365
TrEMBL
other Location (Reliability: 1)
M5AJY0_RAT
308
0
34242
TrEMBL
other Location (Reliability: 2)
A0A8I5XWL7_RAT
700
0
77795
TrEMBL
other Location (Reliability: 1)
D3ZP81_RAT
741
2
81672
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8I6GB48_RAT
253
0
27143
TrEMBL
other Location (Reliability: 1)
A0A8I6AAA7_RAT
806
0
89840
TrEMBL
other Location (Reliability: 1)
A0A8I6AJZ8_RAT
446
0
49814
TrEMBL
other Location (Reliability: 2)
A0A8I5ZTG2_RAT
493
0
53915
TrEMBL
other Location (Reliability: 2)
D3ZY41_RAT
528
0
57808
TrEMBL
other Location (Reliability: 1)
D4A1H7_RAT
352
2
39781
TrEMBL
Secretory Pathway (Reliability: 4)
G3V8S5_RAT
1817
0
200090
TrEMBL
other Location (Reliability: 2)
F1M9Q3_RAT
1798
0
201938
TrEMBL
other Location (Reliability: 4)
A0A0G2JXJ2_RAT
782
0
88197
TrEMBL
other Location (Reliability: 3)
A0A8I5ZLH3_RAT
603
0
67184
TrEMBL
other Location (Reliability: 2)
A0A8I5ZYS0_RAT
392
0
42805
TrEMBL
other Location (Reliability: 4)
A0A140TAB2_RAT
355
0
38616
TrEMBL
other Location (Reliability: 2)
B2RYE1_RAT
418
0
46287
TrEMBL
other Location (Reliability: 2)
A0A8I5ZVP5_RAT
244
1
27463
TrEMBL
Secretory Pathway (Reliability: 1)
Q5XI23_RAT
417
0
46837
TrEMBL
other Location (Reliability: 5)
A0A0G2JSX1_RAT
290
0
33309
TrEMBL
Mitochondrion (Reliability: 1)
A0A8I6AJP0_RAT
409
0
45043
TrEMBL
Mitochondrion (Reliability: 5)
A0A8I5ZK69_RAT
1712
0
195609
TrEMBL
Secretory Pathway (Reliability: 5)
A0A8I6AH15_RAT
679
0
74112
TrEMBL
other Location (Reliability: 2)
D4ACF2_RAT
480
0
55101
TrEMBL
other Location (Reliability: 2)
Q32PY1_RAT
291
0
32328
TrEMBL
other Location (Reliability: 2)
F1LY63_RAT
729
0
82871
TrEMBL
other Location (Reliability: 1)
G3V689_RAT
938
0
104622
TrEMBL
other Location (Reliability: 2)
Q5PQJ8_RAT
663
0
73953
TrEMBL
other Location (Reliability: 2)
A0A8I5ZTQ2_RAT
573
0
62433
TrEMBL
other Location (Reliability: 4)
A0A0G2QC40_RAT
481
0
53088
TrEMBL
other Location (Reliability: 3)
D3ZWU9_RAT
391
0
43993
TrEMBL
other Location (Reliability: 1)
A0A8I5ZSU4_RAT
487
0
52537
TrEMBL
Mitochondrion (Reliability: 4)
D3ZR64_RAT
905
0
99301
TrEMBL
other Location (Reliability: 3)
D4ABZ6_RAT
222
0
25505
TrEMBL
other Location (Reliability: 1)
F1LRJ0_RAT
383
0
42042
TrEMBL
other Location (Reliability: 4)
D3Z8X6_RAT
750
0
83103
TrEMBL
other Location (Reliability: 3)
A0A8I6AG57_RAT
330
1
36652
TrEMBL
other Location (Reliability: 5)
A0A8I6AL90_RAT
453
0
50701
TrEMBL
other Location (Reliability: 5)
A0A0G2K0Z6_RAT
542
0
59488
TrEMBL
other Location (Reliability: 2)
A0A8I6GF45_RAT
725
0
81037
TrEMBL
other Location (Reliability: 5)
D4A1V8_RAT
1174
0
133946
TrEMBL
other Location (Reliability: 1)
A0A8I6AJF0_RAT
547
0
60850
TrEMBL
other Location (Reliability: 1)
A0A8I6G9W9_RAT
380
0
42817
TrEMBL
other Location (Reliability: 4)
A0A8I5Y9P4_RAT
577
0
63379
TrEMBL
other Location (Reliability: 2)
A0A8I5ZL62_RAT
1592
0
181124
TrEMBL
other Location (Reliability: 1)
A0A8I5ZUW9_RAT
230
1
26003
TrEMBL
other Location (Reliability: 5)
A0A8I6AKW4_RAT
552
0
60719
TrEMBL
other Location (Reliability: 2)
A0A8I6G3R2_RAT
914
0
100691
TrEMBL
other Location (Reliability: 2)
A0A8I5ZWP9_RAT
223
0
24360
TrEMBL
other Location (Reliability: 2)
G3XDV3_RAT
790
0
87886
TrEMBL
-
HOIL1_RAT
508
0
57685
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
113800
x * 113800, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 113800, calculated
additional information
isoform Kf-1 interacts with components of the ER-associated degradation pathway, including Derlin-1 and VCP
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
isoform RBCK1 undergoes efficient phosphorylation by protein kinase Cbeta. The phosphorylated RBCK1 shows no self-ubiquitination activity in vitro. Overexpression of protein kinase Cbeta leads to significant increases in the amounts of intracellular RBCK1, presumably suppressing the proteasomal degradation of RBCK1 through self-ubiquitination
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
isoform Rnf4 trimeric complex with UbcH5a and ubiquitin. E2 enzyme UbcH5a is linked by an isopeptide bond to ubiquitin. UbcH5a contacts a single protomer of the RING, and ubiquitin is folded back onto the UbcH5a by contacts from both RING protomers. The C-terminal tail of ubiquitin is locked into an active site groove on UbcH5a by an intricate network of interactions, resulting in changes at the UbcH5a active site
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
a RING finger domain deletion mutant does not show ubiquitin ligase activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in HEK-293 cell
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chin, L.S.; Vavalle, J.P.; Li, L.
Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation
J. Biol. Chem.
277
35071-35079
2002
Rattus norvegicus (Q8CJB9)
Manually annotated by BRENDA team
Maruyama, Y.; Yamada, M.; Takahashi, K.; Yamada, M.
Ubiquitin ligase Kf-1 is involved in the endoplasmic reticulum-associated degradation pathway
Biochem. Biophys. Res. Commun.
374
737-741
2008
Rattus norvegicus (Q9EPZ8)
Manually annotated by BRENDA team
Tatematsu, K.; Yoshimoto, N.; Okajima, T.; Tanizawa, K.; Kuroda, S.
Identification of ubiquitin ligase activity of RBCK1 and its inhibition by splice variant RBCK2 and protein kinase Cbeta
J. Biol. Chem.
283
11575-11585
2008
Rattus norvegicus (Q62921)
Manually annotated by BRENDA team
Iyengar, P.V.; Hirota, T.; Hirose, S.; Nakamura, N.
Membrane-associated RING-CH 10 (MARCH10 protein) is a microtubule-associated E3 ubiquitin ligase of the spermatid flagella
J. Biol. Chem.
286
39082-39090
2011
Rattus norvegicus (G3XDV3)
Manually annotated by BRENDA team
Plechanovova, A.; Jaffray, E.G.; Tatham, M.H.; Naismith, J.H.; Hay, R.T.
Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis
Nature
489
115-120
2012
Rattus norvegicus (O88846)
Manually annotated by BRENDA team