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Information on EC 2.3.2.26 - HECT-type E3 ubiquitin transferase and Organism(s) Rattus norvegicus

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.26 HECT-type E3 ubiquitin transferase
IUBMB Comments
In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. cf. EC 2.3.2.27, RING-type E3 ubiquitin transferase and EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
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This record set is specific for:
Rattus norvegicus
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Word Map
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
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[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E2 ubiquitin-conjugating enzyme]-L-cysteine
+
[acceptor protein]-N6-ubiquitinyl-L-lysine
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E2 ubiquitin-conjugating enzyme]-L-cysteine
+
[acceptor protein]-N6-ubiquitinyl-L-lysine
Synonyms
nedd4, smurf1, smurf2, huwe1, nedd4l, nedd4-1, e6-ap, ube3c, trp120, trip12, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
HECT-type E3 ligase
-
-
HECT-type ubiquitin E3 ligase
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein] ubiquitin transferase (isopeptide bond-forming)
In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. cf. EC 2.3.2.27, RING-type E3 ubiquitin transferase and EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Glis3]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[Glis3]-L-lysine
show the reaction diagram
-
Glis i.e. transcription factor Gli-similar 3
isoform Itch significantly contributes to Glis3 polyubiquitination and reduces Glis3 stability by enhancing its proteasomal degradation. Itch-mediated degradation of Glis3 requires the PPxY motif-dependent interaction between Glis3 and the WW-domains of Itch as well as the presence of the Glis3 zinc finger domains. Itch dramatically inhibits Glis3-mediated transactivation and endogenous Ins2 expression by increasing Glis3 protein turnover
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme knockdown inhibits thioredoxin-interacting protein degradation and results in a subsequent increase in cardiomyocyte apoptosis
physiological function
-
the enzyme targets thioredoxin-interacting protein for ubiquitin-proteasome degradation in cardiomyocytes and ameliorates reactive oxygen species-induced cardiotoxicity through the thioredoxin system. The enzyme protects cardiac remodeling, cardiac function, and survival rate in myocardial infarction
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HERC4_RAT
1057
0
118540
Swiss-Prot
other Location (Reliability: 2)
HUWE1_RAT
322
0
37361
Swiss-Prot
other Location (Reliability: 2)
NEDD4_RAT
887
0
102395
Swiss-Prot
other Location (Reliability: 1)
UBR5_RAT
2788
0
308027
Swiss-Prot
other Location (Reliability: 4)
UBE3D_RAT
370
0
41056
Swiss-Prot
other Location (Reliability: 2)
HACE1_RAT
909
0
102159
Swiss-Prot
Mitochondrion (Reliability: 5)
TRIPC_RAT
2025
0
223928
Swiss-Prot
other Location (Reliability: 5)
D4ADD3_RAT
1578
0
176517
TrEMBL
Mitochondrion (Reliability: 2)
A0A8I5ZKY8_RAT
873
0
100196
TrEMBL
Mitochondrion (Reliability: 5)
Q3KR60_RAT
500
0
57413
TrEMBL
other Location (Reliability: 2)
F1M3F2_RAT
748
0
86094
TrEMBL
Mitochondrion (Reliability: 4)
A0A0G2JUY1_RAT
921
0
105569
TrEMBL
Secretory Pathway (Reliability: 4)
A0A8I6A1F1_RAT
735
0
84488
TrEMBL
Mitochondrion (Reliability: 5)
A0A8I5ZK23_RAT
706
0
80384
TrEMBL
other Location (Reliability: 1)
A0A8I6ANY7_RAT
888
0
102488
TrEMBL
other Location (Reliability: 1)
Q4V8H7_RAT
918
0
104600
TrEMBL
other Location (Reliability: 4)
A0A8I5ZM14_RAT
867
0
100092
TrEMBL
Mitochondrion (Reliability: 5)
A0A8I5ZZU1_RAT
882
0
100831
TrEMBL
Secretory Pathway (Reliability: 4)
M0R8Z5_RAT
1059
0
121395
TrEMBL
Mitochondrion (Reliability: 5)
D4ACN3_RAT
4530
0
493616
TrEMBL
Secretory Pathway (Reliability: 2)
A0A0G2JZS6_RAT
404
1
44888
TrEMBL
other Location (Reliability: 2)
A0A8I6GLU0_RAT
956
0
110141
TrEMBL
other Location (Reliability: 1)
A0A8I6AJU5_RAT
1138
0
128343
TrEMBL
Mitochondrion (Reliability: 5)
A0A8I6AG92_RAT
1603
0
179655
TrEMBL
other Location (Reliability: 5)
A0A0G2K9T1_RAT
864
0
98793
TrEMBL
other Location (Reliability: 1)
A0A8I5ZLT5_RAT
869
0
100363
TrEMBL
other Location (Reliability: 1)
A0A0G2K105_RAT
709
0
80907
TrEMBL
Mitochondrion (Reliability: 4)
A0A0G2K612_RAT
757
0
86104
TrEMBL
Mitochondrion (Reliability: 4)
A0A8I6GFW6_RAT
1223
0
137936
TrEMBL
Mitochondrion (Reliability: 5)
A0A0G2K8P3_RAT
889
0
102754
TrEMBL
other Location (Reliability: 1)
A0A8I6A6J7_RAT
921
0
105487
TrEMBL
other Location (Reliability: 1)
A0A8I6ADN0_RAT
824
0
94175
TrEMBL
Mitochondrion (Reliability: 2)
D3ZLS5_RAT
2610
0
289034
TrEMBL
other Location (Reliability: 4)
A0A8I5ZVG1_RAT
4800
0
523409
TrEMBL
other Location (Reliability: 2)
D3ZHB7_RAT
1083
0
123969
TrEMBL
other Location (Reliability: 4)
F1LRN8_RAT
856
0
98106
TrEMBL
other Location (Reliability: 5)
F7FIK1_RAT
968
0
110488
TrEMBL
other Location (Reliability: 2)
B4F767_RAT
870
0
98778
TrEMBL
Mitochondrion (Reliability: 2)
D7NIW0_RAT
968
0
111495
TrEMBL
other Location (Reliability: 3)
A0A1B0GWW0_RAT
1065
0
121691
TrEMBL
other Location (Reliability: 2)
A0A0G2JVW5_RAT
3941
0
434080
TrEMBL
other Location (Reliability: 4)
A0A8I6GKS9_RAT
942
0
107879
TrEMBL
other Location (Reliability: 3)
A0A0A0MXY4_RAT
1950
0
215446
TrEMBL
other Location (Reliability: 5)
A0A8I6AEH7_RAT
1071
0
121654
TrEMBL
other Location (Reliability: 5)
A0A0G2JUC9_RAT
4836
0
527477
TrEMBL
other Location (Reliability: 2)
A0A8I6AVL7_RAT
855
0
98045
TrEMBL
other Location (Reliability: 5)
A0A8I5ZNU0_RAT
2549
0
281986
TrEMBL
other Location (Reliability: 4)
F1M7B8_RAT
868
0
99753
TrEMBL
other Location (Reliability: 3)
Q5YB86_RAT
854
0
97746
TrEMBL
other Location (Reliability: 3)
A0A8I6A634_RAT
4378
0
482724
TrEMBL
other Location (Reliability: 3)
Q5U1Z5_RAT
440
0
51737
TrEMBL
other Location (Reliability: 2)
A0A8I5ZXL3_RAT
999
0
114593
TrEMBL
Mitochondrion (Reliability: 5)
A0A8I5ZUV2_RAT
695
0
80022
TrEMBL
Mitochondrion (Reliability: 4)
A0A8I6AIV5_RAT
872
0
100886
TrEMBL
other Location (Reliability: 1)
A0A8I6GL04_RAT
912
0
105041
TrEMBL
other Location (Reliability: 1)
A0A1W2Q676_RAT
1966
0
217154
TrEMBL
other Location (Reliability: 5)
A0A8I6A475_RAT
1588
0
177938
TrEMBL
Mitochondrion (Reliability: 2)
F1M4Q8_RAT
1594
0
178726
TrEMBL
Mitochondrion (Reliability: 2)
A0A0G2K6U6_RAT
1321
0
146772
TrEMBL
Mitochondrion (Reliability: 2)
D3ZCF5_RAT
718
0
81768
TrEMBL
other Location (Reliability: 5)
A0A8I6AHN4_RAT
2067
0
228617
TrEMBL
other Location (Reliability: 4)
A0A0G2JU73_RAT
2038
0
225676
TrEMBL
other Location (Reliability: 4)
A0A8I5ZQP0_RAT
1602
0
179621
TrEMBL
Mitochondrion (Reliability: 4)
A0A8I6A4L5_RAT
976
0
112200
TrEMBL
other Location (Reliability: 1)
A0A0G2K0B4_RAT
867
0
100398
TrEMBL
other Location (Reliability: 1)
A0A8I6APS3_RAT
4454
0
489705
TrEMBL
other Location (Reliability: 3)
A0A0G2K0E7_RAT
1070
0
122617
TrEMBL
Mitochondrion (Reliability: 5)
A0A8I5Y0P5_RAT
756
1
87314
TrEMBL
Secretory Pathway (Reliability: 1)
E9PSZ5_RAT
4320
0
474331
TrEMBL
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
113000
-
x * 113000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 113000, SDS-PAGE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is increased 3 h after Dox stimulation (0.0005 mM)
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
ZeRuth, G.T.; Williams, J.G.; Cole, Y.C.; Jetten, A.M.
HECT E3 ubiquitin ligase Itch functions as a novel negative regulator of Gli-Similar 3 (Glis3) transcriptional activity
PLoS ONE
10
e0131303
2015
Rattus norvegicus
Manually annotated by BRENDA team
Otaki, Y.; Takahashi, H.; Watanabe, T.; Funayama, A.; Netsu, S.; Honda, Y.; Narumi, T.; Kadowaki, S.; Hasegawa, H.; Honda, S.; Arimoto, T.; Shishido, T.; Miyamoto, T.; Kamata, H.; Nakajima, O.; Kubota, I.
HECT-type ubiquitin E3 ligase ITCH interacts with thioredoxin-interacting protein and ameliorates reactive oxygen species-induced cardiotoxicity
J. Am. Heart Assoc.
5
e002485
2016
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team