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IUBMB CommentsIn the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. cf. EC 2.3.2.27, RING-type E3 ubiquitin transferase and EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
Synonyms
nedd4, smurf1, smurf2, huwe1, nedd4l, nedd4-1, e6-ap, ube3c, trp120, trip12,
more
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malfunction
-
enzyme knockdown in C2C12 myotubes results in a concurrent increase in Sox6 protein levels and a decrease of Myh7 transcription
malfunction
-
enzyme knockdown inhibits thioredoxin-interacting protein degradation and results in a subsequent increase in cardiomyocyte apoptosis
physiological function
ubiquitin ligase UBR5 specifically enhances trans-activation of smooth muscle-specific promoters by the myocardin family of proteins. UBR5 significantly augments the ability of myocardin to induce expression of endogenous smooth muscle cell marker genes independent on its E3 ligase function. Depletion of endogenous UBR5 by small interfering RNA in fibroblast cells attenuates myocardin-induced smooth muscle-specific gene expression, and UBR5 knockdown in smooth muscle cells results in down-regulation of smooth muscle-specific genes. UBR5 can attenuate myocardin protein degradation resulting in increased myocardin protein expression without affecting myocardin mRNA expression. The effects of UBR5 on myocardin requires only the HECT and UBR1 domains of UBR5
physiological function
-
the enzyme targets thioredoxin-interacting protein for ubiquitin-proteasome degradation in cardiomyocytes and ameliorates reactive oxygen species-induced cardiotoxicity through the thioredoxin system. The enzyme protects cardiac remodeling, cardiac function, and survival rate in myocardial infarction
physiological function
-
the enzyme targets transcription factor Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells
physiological function
Smurf2 mediates [D-Ala2,NMePhe4,Gly5-ol]-enkephalin (DAMGO, an agonist of MOR1)-induced MOR1 ubiquitination and degradation. DAMGO decreases MOR1 levels in the ubiquitin-proteasome system. MOR1 is modified by a Lys48-linked polyubiquitin chain. Overexpression of Smurf2 induces MOR1 ubiquitination and accelerates DAMGO-induced MOR1 degradation, whereas downregulation of Smurf2 attenuates MOR1 degradation. DAMGO increases lung epithelial cell migration and proliferation, and the effect is attenuated by overexpressing Smurf2
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HERC4_MOUSE
1057
0
118412
Swiss-Prot
other Location (Reliability: 2)
HERC2_MOUSE
4836
0
527456
Swiss-Prot
other Location (Reliability: 2)
WWP1_MOUSE
918
0
104694
Swiss-Prot
other Location (Reliability: 4)
G2E3_MOUSE
716
0
81784
Swiss-Prot
other Location (Reliability: 1)
ITCH_MOUSE
864
0
98994
Swiss-Prot
other Location (Reliability: 2)
HECD1_MOUSE
2618
0
290086
Swiss-Prot
other Location (Reliability: 4)
HECD2_MOUSE
774
0
87773
Swiss-Prot
other Location (Reliability: 2)
HECD3_MOUSE
861
0
97347
Swiss-Prot
Mitochondrion (Reliability: 2)
HECW1_MOUSE
1604
0
179467
Swiss-Prot
Mitochondrion (Reliability: 4)
HECW2_MOUSE
1578
0
176235
Swiss-Prot
Mitochondrion (Reliability: 2)
HUWE1_MOUSE
4377
0
482635
Swiss-Prot
other Location (Reliability: 3)
NEDD4_MOUSE
887
0
102706
Swiss-Prot
other Location (Reliability: 1)
AREL1_MOUSE
823
1
94199
Swiss-Prot
Secretory Pathway (Reliability: 1)
UBR5_MOUSE
2792
0
308352
Swiss-Prot
other Location (Reliability: 1)
WWP2_MOUSE
870
0
98761
Swiss-Prot
Mitochondrion (Reliability: 3)
SMUF1_MOUSE
731
0
83356
Swiss-Prot
Mitochondrion (Reliability: 4)
SMUF2_MOUSE
748
0
86175
Swiss-Prot
Mitochondrion (Reliability: 4)
UBE3A_MOUSE
870
0
99819
Swiss-Prot
other Location (Reliability: 3)
UBE3B_MOUSE
1070
0
122762
Swiss-Prot
Mitochondrion (Reliability: 3)
UBE3C_MOUSE
1083
0
123976
Swiss-Prot
other Location (Reliability: 4)
UBE3D_MOUSE
368
0
40753
Swiss-Prot
other Location (Reliability: 5)
HACE1_MOUSE
909
0
102114
Swiss-Prot
Mitochondrion (Reliability: 5)
NED4L_MOUSE
1004
0
115419
Swiss-Prot
other Location (Reliability: 1)
TRIPC_MOUSE
2025
0
224128
Swiss-Prot
other Location (Reliability: 5)
Q3TXI7_MOUSE
431
0
51060
TrEMBL
other Location (Reliability: 1)
Q5EBQ8_MOUSE
119
0
13283
TrEMBL
other Location (Reliability: 2)
Q3TEQ5_MOUSE
243
1
27696
TrEMBL
Secretory Pathway (Reliability: 1)
Q641N8_MOUSE
875
0
101627
TrEMBL
other Location (Reliability: 3)
Q3TQK9_MOUSE
896
0
103613
TrEMBL
other Location (Reliability: 4)
E9PXB7_MOUSE
835
0
96396
TrEMBL
other Location (Reliability: 5)
A0A0B4J1N9_MOUSE
982
0
106459
TrEMBL
other Location (Reliability: 5)
C6EQH2_MOUSE
4746
0
517328
TrEMBL
other Location (Reliability: 5)
Q3UV52_MOUSE
918
0
104621
TrEMBL
other Location (Reliability: 4)
A0A494BAX0_MOUSE
1004
0
115409
TrEMBL
other Location (Reliability: 1)
A0A0G2JDR9_MOUSE
644
0
74081
TrEMBL
other Location (Reliability: 4)
Q91Z48_MOUSE
149
0
16816
TrEMBL
other Location (Reliability: 2)
Q571M5_MOUSE
876
0
100246
TrEMBL
other Location (Reliability: 2)
Q4JG03_MOUSE
4378
0
482746
TrEMBL
other Location (Reliability: 3)
A0A494B9L5_MOUSE
751
0
87141
TrEMBL
other Location (Reliability: 5)
A0A494BA39_MOUSE
872
0
100906
TrEMBL
other Location (Reliability: 1)
B9EKB3_MOUSE
918
0
104708
TrEMBL
other Location (Reliability: 4)
A0A494B953_MOUSE
956
0
110161
TrEMBL
other Location (Reliability: 1)
S4R2P2_MOUSE
243
0
27783
TrEMBL
Mitochondrion (Reliability: 3)
E9PYU8_MOUSE
757
0
86114
TrEMBL
Mitochondrion (Reliability: 4)
Q3UJU3_MOUSE
824
0
94100
TrEMBL
Mitochondrion (Reliability: 3)
O88380_MOUSE
70
0
8254
TrEMBL
other Location (Reliability: 3)
Q05BY9_MOUSE
499
0
54309
TrEMBL
other Location (Reliability: 4)
Q3T9S4_MOUSE
642
0
70104
TrEMBL
other Location (Reliability: 4)
G3X9H8_MOUSE
855
0
98456
TrEMBL
other Location (Reliability: 5)
D3Z587_MOUSE
138
0
14535
TrEMBL
other Location (Reliability: 4)
Q9D0H0_MOUSE
368
0
40750
TrEMBL
other Location (Reliability: 5)
A0A0G2JG76_MOUSE
178
0
20265
TrEMBL
other Location (Reliability: 3)
Q3TEJ5_MOUSE
494
0
53610
TrEMBL
other Location (Reliability: 4)
I3RSH5_MOUSE
870
0
98761
TrEMBL
Mitochondrion (Reliability: 3)
A0A087WSG4_MOUSE
213
0
22804
TrEMBL
Mitochondrion (Reliability: 3)
E9Q4K9_MOUSE
728
0
83110
TrEMBL
Mitochondrion (Reliability: 4)
Q3UQY6_MOUSE
639
0
68620
TrEMBL
other Location (Reliability: 4)
A0A494BAG2_MOUSE
976
0
112220
TrEMBL
other Location (Reliability: 1)
Q3U5E1_MOUSE
763
0
83552
TrEMBL
other Location (Reliability: 4)
Q8R0U4_MOUSE
399
0
45401
TrEMBL
other Location (Reliability: 5)
A0A494B9F0_MOUSE
912
0
105061
TrEMBL
other Location (Reliability: 1)
B2RSC8_MOUSE
887
0
102706
TrEMBL
other Location (Reliability: 1)
A0A1Y7VJ05_MOUSE
1177
0
131935
TrEMBL
other Location (Reliability: 1)
Q6P289_MOUSE
806
0
92438
TrEMBL
other Location (Reliability: 2)
Q8C5S2_MOUSE
308
0
34794
TrEMBL
other Location (Reliability: 1)
F8WIE5_MOUSE
2610
0
289229
TrEMBL
other Location (Reliability: 4)
Q05CE4_MOUSE
787
0
90083
TrEMBL
other Location (Reliability: 2)
A0A087WNZ7_MOUSE
1992
0
220661
TrEMBL
other Location (Reliability: 5)
A0A494BAE4_MOUSE
527
0
61660
TrEMBL
other Location (Reliability: 5)
A0A087WRV6_MOUSE
830
0
94901
TrEMBL
other Location (Reliability: 3)
A0A1D5RM92_MOUSE
824
0
94158
TrEMBL
Mitochondrion (Reliability: 3)
A2AFQ0_MOUSE
4378
0
482692
TrEMBL
other Location (Reliability: 3)
A0A0G2JEN5_MOUSE
309
0
35353
TrEMBL
other Location (Reliability: 1)
A0A571BDN7_MOUSE
1136
0
127651
TrEMBL
Mitochondrion (Reliability: 5)
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Hu, G.; Wang, X.; Saunders, D.; Henderson, M.; Russell, A.; Herring, B.; Zhou, J.
Modulation of myocardin function by the ubiquitin E3 ligase UBR5
J. Biol. Chem.
286
11800-11809
2011
Mus musculus (Q80TP3)
brenda
Otaki, Y.; Takahashi, H.; Watanabe, T.; Funayama, A.; Netsu, S.; Honda, Y.; Narumi, T.; Kadowaki, S.; Hasegawa, H.; Honda, S.; Arimoto, T.; Shishido, T.; Miyamoto, T.; Kamata, H.; Nakajima, O.; Kubota, I.
HECT-type ubiquitin E3 ligase ITCH interacts with thioredoxin-interacting protein and ameliorates reactive oxygen species-induced cardiotoxicity
J. Am. Heart Assoc.
5
e002485
2016
Mus musculus, Rattus norvegicus
brenda
An, C.I.; Ganio, E.; Hagiwara, N.
Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells
Skeletal Muscle
3
11
2013
Homo sapiens (Q14669), Mus musculus
brenda
Dong, S.; Liu, J.; Li, L.; Wang, H.; Ma, H.; Zhao, Y.; Zhao, J.
The HECT ubiquitin E3 ligase Smurf2 degrades my-opioid receptor 1 in the ubiquitin-proteasome system in lung epithelial cells
Am. J. Physiol. Cell Physiol.
316
C632-C640
2019
Mus musculus (A2A5Z6)
brenda