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Information on EC 2.3.2.26 - HECT-type E3 ubiquitin transferase and Organism(s) Drosophila melanogaster

for references in articles please use BRENDA:EC2.3.2.26
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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.26 HECT-type E3 ubiquitin transferase
IUBMB Comments
In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. cf. EC 2.3.2.27, RING-type E3 ubiquitin transferase and EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
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This record set is specific for:
Drosophila melanogaster
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
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[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E2 ubiquitin-conjugating enzyme]-L-cysteine
+
[acceptor protein]-N6-ubiquitinyl-L-lysine
Synonyms
nedd4, smurf1, smurf2, huwe1, nedd4l, nedd4-1, e6-ap, ube3c, trp120, trip12, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein] ubiquitin transferase (isopeptide bond-forming)
In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. cf. EC 2.3.2.27, RING-type E3 ubiquitin transferase and EC 2.3.2.31, RBR-type E3 ubiquitin transferase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Sav]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[Sav]-L-lysine
show the reaction diagram
-
Sav i.e. scaffold protein Salvador, believed to promote Hpo/Wts association
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
isoform Herc4 expression promotes scaffold protein Sav ubiquitylation and degradation, while Herc4 depletion stabilises Sav. Sav-binding protein Hpo reduces Sav/Herc4 interaction in a kinase-dependent manner
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HERC2_DROME
4912
0
529997
Swiss-Prot
Mitochondrion (Reliability: 4)
HYD_DROME
2885
0
318881
Swiss-Prot
other Location (Reliability: 2)
NEDD4_DROME
1007
0
114875
Swiss-Prot
Mitochondrion (Reliability: 3)
UFD4_DROME
2727
0
302150
Swiss-Prot
other Location (Reliability: 4)
SMUF1_DROME
1061
0
115676
Swiss-Prot
Mitochondrion (Reliability: 5)
SUDX_DROME
949
0
107966
Swiss-Prot
other Location (Reliability: 2)
A0A0B4JCV6_DROME
2930
0
314167
TrEMBL
other Location (Reliability: 1)
M9MS90_DROME
1423
0
156664
TrEMBL
other Location (Reliability: 4)
A0A0B4KFU5_DROME
1061
0
115676
TrEMBL
Mitochondrion (Reliability: 5)
A0A0B4JCW5_DROME
2929
0
314096
TrEMBL
other Location (Reliability: 1)
C0PUW9_DROME
488
0
57188
TrEMBL
other Location (Reliability: 2)
X2JC16_DROME
5150
0
557318
TrEMBL
other Location (Reliability: 2)
Q9VSS9_DROME
1078
0
123777
TrEMBL
other Location (Reliability: 3)
M9PEV2_DROME
1077
0
123706
TrEMBL
other Location (Reliability: 3)
F6M9W1_DROME
2708
0
289615
TrEMBL
other Location (Reliability: 1)
Q6NNZ7_DROME
561
0
62366
TrEMBL
other Location (Reliability: 2)
Q9W326_DROME
1426
0
156919
TrEMBL
other Location (Reliability: 4)
B7Z067_DROME
983
0
111895
TrEMBL
Mitochondrion (Reliability: 3)
A0A0B4JDB5_DROME
2737
0
292503
TrEMBL
other Location (Reliability: 1)
Q9VN58_DROME
3140
0
336576
TrEMBL
other Location (Reliability: 1)
M9PFY9_DROME
999
0
113643
TrEMBL
Mitochondrion (Reliability: 3)
Q8IQR5_DROME
931
0
106238
TrEMBL
Mitochondrion (Reliability: 3)
X2JFK0_DROME
5145
0
556773
TrEMBL
other Location (Reliability: 3)
A0A0B4LGR2_DROME
3111
0
333702
TrEMBL
other Location (Reliability: 1)
Q9W196_DROME
1122
0
128944
TrEMBL
other Location (Reliability: 3)
X2JKH9_DROME
5127
0
555282
TrEMBL
other Location (Reliability: 3)
A0A0B4LGQ5_DROME
2901
0
311292
TrEMBL
other Location (Reliability: 1)
X2JF83_DROME
5101
0
553063
TrEMBL
other Location (Reliability: 3)
A0A0B4JDA1_DROME
3139
0
336505
TrEMBL
other Location (Reliability: 1)
Q9VXR3_DROME
5146
0
556860
TrEMBL
other Location (Reliability: 3)
F6M9W0_DROME
3140
0
336571
TrEMBL
other Location (Reliability: 1)
X2JDY2_DROME
5116
0
554350
TrEMBL
other Location (Reliability: 3)
A0A0B4LHQ4_DROME
2708
0
289629
TrEMBL
other Location (Reliability: 1)
M9PFK2_DROME
1005
0
114648
TrEMBL
Mitochondrion (Reliability: 3)
B7Z068_DROME
994
0
113366
TrEMBL
Mitochondrion (Reliability: 3)
F6M9W2_DROME
2930
0
314162
TrEMBL
other Location (Reliability: 1)
X2JF73_DROME
5151
0
557628
TrEMBL
other Location (Reliability: 3)
A0A0B4JD62_DROME
2736
0
292432
TrEMBL
other Location (Reliability: 1)
B7YZY9_DROME
949
0
107966
TrEMBL
other Location (Reliability: 2)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Aerne, B.L.; Gailite, I.; Sims, D.; Tapon, N.
Hippo stabilises its adaptor Salvador by antagonising the HECT ubiquitin ligase Herc4
PLoS ONE
10
e0131113
2015
Drosophila melanogaster, Drosophila melanogaster S2
Manually annotated by BRENDA team